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The map label for this gene is kefB [H]

Identifier: 119946263

GI number: 119946263

Start: 3220514

End: 3222112

Strand: Direct

Name: kefB [H]

Synonym: Ping_2626

Alternate gene names: 119946263

Gene position: 3220514-3222112 (Clockwise)

Preceding gene: 119946262

Following gene: 119946266

Centisome position: 70.63

GC content: 35.27

Gene sequence:

>1599_bases
ATGGACTCTATTCTTATTATCATCTTCATTTCATTAGCAATTGCTAGCATATTAAATATTGTGCTAAAAAAATTCTCCGT
ATCACACATTATTGGTTACATTATCACCGGTACTATTATCAGTAATATTTTTGACTTTAATGGCAGTGAAGACCTTAACT
CATTAGAGCTTATTGGTGAATTTGGCATCGTCTTTTTAATGTTCACCATTGGCTTAGAGTTGTCCTTTTCTAAATTAAAT
AAAATGAAAGAGATCATTTTCTTTAATGGTTTTATACAAGTCGCTTTAAGCTCAGTGATTATTTTTATGCTTTCATACTA
CCTGTTTAATATTGACAGCACTTCCTCTATTATTATTGCACTGGCTTTTAGCTTGTCCTCCACGGCAATAGTCTTAACTT
ATTTAAAAAAATCAAAAGATATTATCACCCCTTATGGCACCAAATCCGTTGCTATCTTAGTCTTTCAGGATTTGGCTGTT
ATTCCTATTTTATTGCTGATTAGTTTTTTAGCCAATGATACTTTATCAATAACCGATGTGTTATTCAAAACCGCAATATC
AGCGACTTTAGTCATTGTTTTCATGTTTACCTTAGGTAAAAAAATAATCGAATGGCTGCTGCATTTTTCCAGCACCGCCA
GGTTGGATGAATTATTTTTAGCCTCAGTATTAACCATTGTGGTGGGCGCCTCGCTTTTAGCACACGAAATGGGATTTACC
TACTCGTTAGGCTCCTTTATTGCAGGTATGATTATTGCCGGAACCACTTATCATGTAAAAGTGGAGTCAGATATCGCCTC
TTATAAAGACCTTTTATTAGGCGCATTTTTCTTTTCTATCGGCACCAAAATTGACATCTTTTATTTCGCAAGCCATTTAC
ATTTAGTGTTTGCGGTATTAGCTTTTACCATGTTAATAAAAGCGCTGATTATTTATTTTTTAATTCGTAAAAAATCTAAT
AAAAGTGATTCACTTAAATCGGCCATTGCACTTTGCCAAGTCGGTGAGTTTTCTTTTGCTATTTTCTCGATTGCCATTAG
CCAAGAAATCATCACGCCTGAACTGGGCAGTTTCTTAATTTTAGTGGCGGTTTCCTCTATGATTGTTACGCCCTTTATGG
TCAATAATATTTATAAAATAGCCTCTTTATTTGTAGTGGAGTTTTTTGAATCCGATAAAATTACCCCGATCAAGGAGACT
AATCATACCGTTATTTGTGGCTTTGCGATCCTCGGGCGACTGGTAGCAAAAGAGTTAGAAAAAGAAGGACGGCAGTTTTT
AATTATCTCAGATAATTTACAACATGTATTGGAGGCAAGAAAACGTGGATATATGGCTTATTTTGGTCATTTAGAGAAAC
ACCCGGTATTAGAATCACTAAAAATAGAGCAGTCTTCCAGTATTATTATTACCGTCAATACCGCCAGAACCAAAAAAATT
ATCTGCGAAGCGGTACTCGATTATTATCCTCAAGCAAACTTAGTGGTCAGAATTAACAGTCTCGAGGAAAAAAAGACCTT
GGCGAATCTGAATGTTCACTATTTTGTTCATGCTCAGCATGAAACCGCACTCCTGCTGGTTCAAAAAAGTCTCCGTTAA

Upstream 100 bases:

>100_bases
AAAAATTAAATTTATATTAACACCTACATTTTCCGGTATGGTTTTTGCTTATTCCTAAAGAGCCACCACAACTTATTATT
TGAAGCTAAGAGAGAAAAAA

Downstream 100 bases:

>100_bases
TACACTAAATAAAGTATTTTTACCTCTCCATTAATGCGTTAGAGACTAAAAAACCAGAGTGAATTATTCACCCTGGTTTT
TTATCTCTGTTCAGATTAAA

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MDSILIIIFISLAIASILNIVLKKFSVSHIIGYIITGTIISNIFDFNGSEDLNSLELIGEFGIVFLMFTIGLELSFSKLN
KMKEIIFFNGFIQVALSSVIIFMLSYYLFNIDSTSSIIIALAFSLSSTAIVLTYLKKSKDIITPYGTKSVAILVFQDLAV
IPILLLISFLANDTLSITDVLFKTAISATLVIVFMFTLGKKIIEWLLHFSSTARLDELFLASVLTIVVGASLLAHEMGFT
YSLGSFIAGMIIAGTTYHVKVESDIASYKDLLLGAFFFSIGTKIDIFYFASHLHLVFAVLAFTMLIKALIIYFLIRKKSN
KSDSLKSAIALCQVGEFSFAIFSIAISQEIITPELGSFLILVAVSSMIVTPFMVNNIYKIASLFVVEFFESDKITPIKET
NHTVICGFAILGRLVAKELEKEGRQFLIISDNLQHVLEARKRGYMAYFGHLEKHPVLESLKIEQSSSIIITVNTARTKKI
ICEAVLDYYPQANLVVRINSLEEKKTLANLNVHYFVHAQHETALLLVQKSLR

Sequences:

>Translated_532_residues
MDSILIIIFISLAIASILNIVLKKFSVSHIIGYIITGTIISNIFDFNGSEDLNSLELIGEFGIVFLMFTIGLELSFSKLN
KMKEIIFFNGFIQVALSSVIIFMLSYYLFNIDSTSSIIIALAFSLSSTAIVLTYLKKSKDIITPYGTKSVAILVFQDLAV
IPILLLISFLANDTLSITDVLFKTAISATLVIVFMFTLGKKIIEWLLHFSSTARLDELFLASVLTIVVGASLLAHEMGFT
YSLGSFIAGMIIAGTTYHVKVESDIASYKDLLLGAFFFSIGTKIDIFYFASHLHLVFAVLAFTMLIKALIIYFLIRKKSN
KSDSLKSAIALCQVGEFSFAIFSIAISQEIITPELGSFLILVAVSSMIVTPFMVNNIYKIASLFVVEFFESDKITPIKET
NHTVICGFAILGRLVAKELEKEGRQFLIISDNLQHVLEARKRGYMAYFGHLEKHPVLESLKIEQSSSIIITVNTARTKKI
ICEAVLDYYPQANLVVRINSLEEKKTLANLNVHYFVHAQHETALLLVQKSLR
>Mature_532_residues
MDSILIIIFISLAIASILNIVLKKFSVSHIIGYIITGTIISNIFDFNGSEDLNSLELIGEFGIVFLMFTIGLELSFSKLN
KMKEIIFFNGFIQVALSSVIIFMLSYYLFNIDSTSSIIIALAFSLSSTAIVLTYLKKSKDIITPYGTKSVAILVFQDLAV
IPILLLISFLANDTLSITDVLFKTAISATLVIVFMFTLGKKIIEWLLHFSSTARLDELFLASVLTIVVGASLLAHEMGFT
YSLGSFIAGMIIAGTTYHVKVESDIASYKDLLLGAFFFSIGTKIDIFYFASHLHLVFAVLAFTMLIKALIIYFLIRKKSN
KSDSLKSAIALCQVGEFSFAIFSIAISQEIITPELGSFLILVAVSSMIVTPFMVNNIYKIASLFVVEFFESDKITPIKET
NHTVICGFAILGRLVAKELEKEGRQFLIISDNLQHVLEARKRGYMAYFGHLEKHPVLESLKIEQSSSIIITVNTARTKKI
ICEAVLDYYPQANLVVRINSLEEKKTLANLNVHYFVHAQHETALLLVQKSLR

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=359, Percent_Identity=28.4122562674095, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786232, Length=528, Percent_Identity=27.2727272727273, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789749, Length=527, Percent_Identity=27.5142314990512, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786685, Length=531, Percent_Identity=24.2937853107345, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 59224; Mature: 59224

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSILIIIFISLAIASILNIVLKKFSVSHIIGYIITGTIISNIFDFNGSEDLNSLELIGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
FGIVFLMFTIGLELSFSKLNKMKEIIFFNGFIQVALSSVIIFMLSYYLFNIDSTSSIIIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHH
LAFSLSSTAIVLTYLKKSKDIITPYGTKSVAILVFQDLAVIPILLLISFLANDTLSITDV
EEHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LFKTAISATLVIVFMFTLGKKIIEWLLHFSSTARLDELFLASVLTIVVGASLLAHEMGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YSLGSFIAGMIIAGTTYHVKVESDIASYKDLLLGAFFFSIGTKIDIFYFASHLHLVFAVL
EEHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHH
AFTMLIKALIIYFLIRKKSNKSDSLKSAIALCQVGEFSFAIFSIAISQEIITPELGSFLI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHH
LVAVSSMIVTPFMVNNIYKIASLFVVEFFESDKITPIKETNHTVICGFAILGRLVAKELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
KEGRQFLIISDNLQHVLEARKRGYMAYFGHLEKHPVLESLKIEQSSSIIITVNTARTKKI
HCCCEEEEEECCHHHHHHHHHCCCCHHHCCCCCCCCHHHHEECCCCCEEEEEECCHHHHH
ICEAVLDYYPQANLVVRINSLEEKKTLANLNVHYFVHAQHETALLLVQKSLR
HHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEEEEEEECCCCEEEHEEHHCC
>Mature Secondary Structure
MDSILIIIFISLAIASILNIVLKKFSVSHIIGYIITGTIISNIFDFNGSEDLNSLELIGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
FGIVFLMFTIGLELSFSKLNKMKEIIFFNGFIQVALSSVIIFMLSYYLFNIDSTSSIIIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHH
LAFSLSSTAIVLTYLKKSKDIITPYGTKSVAILVFQDLAVIPILLLISFLANDTLSITDV
EEHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LFKTAISATLVIVFMFTLGKKIIEWLLHFSSTARLDELFLASVLTIVVGASLLAHEMGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YSLGSFIAGMIIAGTTYHVKVESDIASYKDLLLGAFFFSIGTKIDIFYFASHLHLVFAVL
EEHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHH
AFTMLIKALIIYFLIRKKSNKSDSLKSAIALCQVGEFSFAIFSIAISQEIITPELGSFLI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHH
LVAVSSMIVTPFMVNNIYKIASLFVVEFFESDKITPIKETNHTVICGFAILGRLVAKELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
KEGRQFLIISDNLQHVLEARKRGYMAYFGHLEKHPVLESLKIEQSSSIIITVNTARTKKI
HCCCEEEEEECCHHHHHHHHHCCCCHHHCCCCCCCCHHHHEECCCCCEEEEEECCHHHHH
ICEAVLDYYPQANLVVRINSLEEKKTLANLNVHYFVHAQHETALLLVQKSLR
HHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEEEEEEECCCCEEEHEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA