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The map label for this gene is topB [H]

Identifier: 119946111

GI number: 119946111

Start: 3010993

End: 3012924

Strand: Reverse

Name: topB [H]

Synonym: Ping_2461

Alternate gene names: 119946111

Gene position: 3012924-3010993 (Counterclockwise)

Preceding gene: 119946114

Following gene: 119946109

Centisome position: 66.08

GC content: 42.91

Gene sequence:

>1932_bases
ATGAAACTTTATATTGCCGAAAAACCAAGCCTGGCACGTGCGATTGCTGATGCGCTACCAAAACCGCATCGTAAAGCAGA
CGGTTGTATTTATGTTGGTAATGGAGATGTTGTAAGCTGGTGCATCGGGCATCTGTTAACTCAGGTTGATCCCGAGGCTT
ATGATCCCGAATATAAAAAATGGAAATTCGAACACCTGCCGATTATTCCCGAAAACTGGAAATTAAAATCAACACCGCGC
ACCAGTAAACAACTGAGTATATTAAAAAAACTGATCAAACAGGCTGATAACCTTGTGCATGTTGGCGACCCCGACCGCGA
AGGCCAGTTGCTTGTGGATGAGGTGATTCATTTCAGCGGTGTTAAGGGCAATAAATTAAAACAGGTTGAGCGCTGTTTGG
TCAGTGATTTAACCACCAATGCGGTAAAACGTTCGCTTCAGCAATTACGCTCTAATCAGGAGTTTATTGCATTATCAACC
TCGGCGCTTGCGAGAACGCGTGCTGACTGGTTATATGGGCTAAATTTGACACGAGTTTATACCTTACAGGCACAAAAGTC
AGGTTATCAGGGGGTTATCTCGGTGGGGCGAGTGCAAACACCTTTATTAGGCTTGGTGGCAAGGCGCGATAAAGAGATTG
AAGTTTTTGTGAGTAAACCCTTTTATGAAGTGGAAGCACACTTAACGACACTCAAACCTGAAAATGTTGCTGCCTTTACG
GCCAAATGGAAGCCCAGTGAAGCCTGCGAAAAATATCAGGATCACGAGGGGCGTGTACTGGTTAAAAAGTTGGCAGAGAA
TGTCGCGCAGCGTATTTCTCAGCAACACGCTGTGGTAAAAAGCTTTACGGCTAAAAATAAAAAGCAGGTTGCGCCTTTAC
CCTATAACTTATCGGCGTTACAGATTGATGCTGCCAAGCGTTTTAGCTACACGGCGCTGGAAGTATTGGATATTTGTCAG
GCATTGTATGAAAAATATAAGCTGATTACTTACCCGCGTTCTGATTCCCGTTATCTGCCTAAAAATCATCTTTCTCAAGC
CGGATCAGTGACCCGGGCGATTGCTAATAACCAGCCTGCTTTAAAAAAGGCGGTCAGTAATGCTGATATTTCTTTGCAGA
GCCGTGCCTGGAACGATAAGAAGGTTGATGCCCATCATGCGATAATTCCAACGGATAAACATCAGTCTTTAACGAGTTTA
GATACTGCCTCACAAAATATTTACGCTTTAATTGCCCGTCAGTATTTATGCCAGTTTTATGCCGATTATCATTACGGTGA
ATGCGTGGCTGAAATTGAAATAAATAACGGCTTATTTATCAGTAAAGCAAAAGCTAAAATCAGCGACGGGTGGAAAGTAT
TATTTGATAAGCAGGAGAAAGACAGTTATTTACCTGCGCTTAGCAAAGGGCAAAAGTTGTTTTGTGATAAAGGTGAGGTG
ATTGAAAAAAACACTCAGCCGCCAGCCTATTTTACGGATGCTACTCTGTTAGCCGCAATGAGTAATATTGCCCGTTATGT
GAAAGACGGAGAATTGAAAGCTGTGTTGAAAGAAACCGATGGTCTGGGCACGGAAGCGACCCGAGCGGGGATTATTGAGC
TGCTGTTTAAACGTTTGTTTCTGCAGCGCACGGGTAAACTTATTAAAGCAACTGAGACCGGCAGAAAGTTAATTGAAAGT
CTGCCGGAAATGTTGACCCTGCCTGATATGACCGCGCAATGGGAGATGGAATTAAATGCTATCAGTCAAAAAGAGCAAAG
TTATCAGGGATTTGTTGTGCCATTACAAAATCAGCTTAAAAGCATTATAGAAAAGGCAATTGCAGACGGGCCGCGGGCAT
TGGCGAATTTACCAAGCAGTGGCAATGTTTTTAAGAAAACCTACAGAAAAAAATCAACAAAAAAAGGCACTTTTAAAAAG
AAAGGCAGTTAA

Upstream 100 bases:

>100_bases
GTCAGTGATAGAGCCTAATAGTTCCATTAATGATGCTTTAAACTTGAATTTTTTATATCAGTAGACTACTGTGTAAAAAA
ACAGTTTAAATGAAGGTGCC

Downstream 100 bases:

>100_bases
TAATGCCCAAAGAAATTAAACTGTGATTTTCCAGCTTCGGGGTTTGGGCTGCGAGTTCCGAGCTTCGGGCTTGCGAGCTT
CGAGCTGAATAGTTATCGGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MKLYIAEKPSLARAIADALPKPHRKADGCIYVGNGDVVSWCIGHLLTQVDPEAYDPEYKKWKFEHLPIIPENWKLKSTPR
TSKQLSILKKLIKQADNLVHVGDPDREGQLLVDEVIHFSGVKGNKLKQVERCLVSDLTTNAVKRSLQQLRSNQEFIALST
SALARTRADWLYGLNLTRVYTLQAQKSGYQGVISVGRVQTPLLGLVARRDKEIEVFVSKPFYEVEAHLTTLKPENVAAFT
AKWKPSEACEKYQDHEGRVLVKKLAENVAQRISQQHAVVKSFTAKNKKQVAPLPYNLSALQIDAAKRFSYTALEVLDICQ
ALYEKYKLITYPRSDSRYLPKNHLSQAGSVTRAIANNQPALKKAVSNADISLQSRAWNDKKVDAHHAIIPTDKHQSLTSL
DTASQNIYALIARQYLCQFYADYHYGECVAEIEINNGLFISKAKAKISDGWKVLFDKQEKDSYLPALSKGQKLFCDKGEV
IEKNTQPPAYFTDATLLAAMSNIARYVKDGELKAVLKETDGLGTEATRAGIIELLFKRLFLQRTGKLIKATETGRKLIES
LPEMLTLPDMTAQWEMELNAISQKEQSYQGFVVPLQNQLKSIIEKAIADGPRALANLPSSGNVFKKTYRKKSTKKGTFKK
KGS

Sequences:

>Translated_643_residues
MKLYIAEKPSLARAIADALPKPHRKADGCIYVGNGDVVSWCIGHLLTQVDPEAYDPEYKKWKFEHLPIIPENWKLKSTPR
TSKQLSILKKLIKQADNLVHVGDPDREGQLLVDEVIHFSGVKGNKLKQVERCLVSDLTTNAVKRSLQQLRSNQEFIALST
SALARTRADWLYGLNLTRVYTLQAQKSGYQGVISVGRVQTPLLGLVARRDKEIEVFVSKPFYEVEAHLTTLKPENVAAFT
AKWKPSEACEKYQDHEGRVLVKKLAENVAQRISQQHAVVKSFTAKNKKQVAPLPYNLSALQIDAAKRFSYTALEVLDICQ
ALYEKYKLITYPRSDSRYLPKNHLSQAGSVTRAIANNQPALKKAVSNADISLQSRAWNDKKVDAHHAIIPTDKHQSLTSL
DTASQNIYALIARQYLCQFYADYHYGECVAEIEINNGLFISKAKAKISDGWKVLFDKQEKDSYLPALSKGQKLFCDKGEV
IEKNTQPPAYFTDATLLAAMSNIARYVKDGELKAVLKETDGLGTEATRAGIIELLFKRLFLQRTGKLIKATETGRKLIES
LPEMLTLPDMTAQWEMELNAISQKEQSYQGFVVPLQNQLKSIIEKAIADGPRALANLPSSGNVFKKTYRKKSTKKGTFKK
KGS
>Mature_643_residues
MKLYIAEKPSLARAIADALPKPHRKADGCIYVGNGDVVSWCIGHLLTQVDPEAYDPEYKKWKFEHLPIIPENWKLKSTPR
TSKQLSILKKLIKQADNLVHVGDPDREGQLLVDEVIHFSGVKGNKLKQVERCLVSDLTTNAVKRSLQQLRSNQEFIALST
SALARTRADWLYGLNLTRVYTLQAQKSGYQGVISVGRVQTPLLGLVARRDKEIEVFVSKPFYEVEAHLTTLKPENVAAFT
AKWKPSEACEKYQDHEGRVLVKKLAENVAQRISQQHAVVKSFTAKNKKQVAPLPYNLSALQIDAAKRFSYTALEVLDICQ
ALYEKYKLITYPRSDSRYLPKNHLSQAGSVTRAIANNQPALKKAVSNADISLQSRAWNDKKVDAHHAIIPTDKHQSLTSL
DTASQNIYALIARQYLCQFYADYHYGECVAEIEINNGLFISKAKAKISDGWKVLFDKQEKDSYLPALSKGQKLFCDKGEV
IEKNTQPPAYFTDATLLAAMSNIARYVKDGELKAVLKETDGLGTEATRAGIIELLFKRLFLQRTGKLIKATETGRKLIES
LPEMLTLPDMTAQWEMELNAISQKEQSYQGFVVPLQNQLKSIIEKAIADGPRALANLPSSGNVFKKTYRKKSTKKGTFKK
KGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=640, Percent_Identity=25, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI4507635, Length=618, Percent_Identity=23.6245954692557, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1788061, Length=646, Percent_Identity=54.4891640866873, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=556, Percent_Identity=26.4388489208633, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=628, Percent_Identity=23.8853503184713, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585251, Length=521, Percent_Identity=27.6391554702495, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640096, Length=620, Percent_Identity=23.7096774193548, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72154; Mature: 72154

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYIAEKPSLARAIADALPKPHRKADGCIYVGNGDVVSWCIGHLLTQVDPEAYDPEYKK
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHC
WKFEHLPIIPENWKLKSTPRTSKQLSILKKLIKQADNLVHVGDPDREGQLLVDEVIHFSG
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCC
VKGNKLKQVERCLVSDLTTNAVKRSLQQLRSNQEFIALSTSALARTRADWLYGLNLTRVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEEE
TLQAQKSGYQGVISVGRVQTPLLGLVARRDKEIEVFVSKPFYEVEAHLTTLKPENVAAFT
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHEECCCCCCEEEE
AKWKPSEACEKYQDHEGRVLVKKLAENVAQRISQQHAVVKSFTAKNKKQVAPLPYNLSAL
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
QIDAAKRFSYTALEVLDICQALYEKYKLITYPRSDSRYLPKNHLSQAGSVTRAIANNQPA
EECHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHH
LKKAVSNADISLQSRAWNDKKVDAHHAIIPTDKHQSLTSLDTASQNIYALIARQYLCQFY
HHHHHCCCCCEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADYHYGECVAEIEINNGLFISKAKAKISDGWKVLFDKQEKDSYLPALSKGQKLFCDKGEV
HCCCCCCEEEEEEECCCEEEEECCHHHCCCEEEEECCCCCCCCCCHHHCCCEEECCCCCC
IEKNTQPPAYFTDATLLAAMSNIARYVKDGELKAVLKETDGLGTEATRAGIIELLFKRLF
CCCCCCCCCEECHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LQRTGKLIKATETGRKLIESLPEMLTLPDMTAQWEMELNAISQKEQSYQGFVVPLQNQLK
HHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHH
SIIEKAIADGPRALANLPSSGNVFKKTYRKKSTKKGTFKKKGS
HHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLYIAEKPSLARAIADALPKPHRKADGCIYVGNGDVVSWCIGHLLTQVDPEAYDPEYKK
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHC
WKFEHLPIIPENWKLKSTPRTSKQLSILKKLIKQADNLVHVGDPDREGQLLVDEVIHFSG
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCC
VKGNKLKQVERCLVSDLTTNAVKRSLQQLRSNQEFIALSTSALARTRADWLYGLNLTRVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEEE
TLQAQKSGYQGVISVGRVQTPLLGLVARRDKEIEVFVSKPFYEVEAHLTTLKPENVAAFT
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHEECCCCCCEEEE
AKWKPSEACEKYQDHEGRVLVKKLAENVAQRISQQHAVVKSFTAKNKKQVAPLPYNLSAL
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
QIDAAKRFSYTALEVLDICQALYEKYKLITYPRSDSRYLPKNHLSQAGSVTRAIANNQPA
EECHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHH
LKKAVSNADISLQSRAWNDKKVDAHHAIIPTDKHQSLTSLDTASQNIYALIARQYLCQFY
HHHHHCCCCCEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADYHYGECVAEIEINNGLFISKAKAKISDGWKVLFDKQEKDSYLPALSKGQKLFCDKGEV
HCCCCCCEEEEEEECCCEEEEECCHHHCCCEEEEECCCCCCCCCCHHHCCCEEECCCCCC
IEKNTQPPAYFTDATLLAAMSNIARYVKDGELKAVLKETDGLGTEATRAGIIELLFKRLF
CCCCCCCCCEECHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LQRTGKLIKATETGRKLIESLPEMLTLPDMTAQWEMELNAISQKEQSYQGFVVPLQNQLK
HHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHH
SIIEKAIADGPRALANLPSSGNVFKKTYRKKSTKKGTFKKKGS
HHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]