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The map label for this gene is mptN [H]

Identifier: 119946109

GI number: 119946109

Start: 3008156

End: 3009118

Strand: Reverse

Name: mptN [H]

Synonym: Ping_2459

Alternate gene names: 119946109

Gene position: 3009118-3008156 (Counterclockwise)

Preceding gene: 119946111

Following gene: 119946108

Centisome position: 66.0

GC content: 35.93

Gene sequence:

>963_bases
ATGAGTAGTAATAAAAGGATAGGCTCAGAACCAACGGTTGGAATATGGTTATATAAAAACGGTGGGGGGATTGAAATACA
GCAAAAATTAATCGAGATTCTTACAAAAAAAGGCATAAGGACCATAACAGATCTCAATCTCGGACATGCTTATGCATCTG
GAGGTGAAATTATTTGTAACGGTATTAATATGGAAAACTTAAACGCTTTTTTCTCCTATAATGCAGGAGAGCAATCAACC
TTTCAATTATATATGTATCAATTACTTAATCAAACGACTTCTACCCTTAATAATTATGATGCTTTTGCTTTGACTGAAGA
TAAGTTTTTAACCGCTCATGCATTAAATCATGCCGGAATAAGAACAGCTGAATATCGCATGATTCATTGTGATGATATTT
CATTATTAAAAAAAACAGTCAGTGATTGGCAAGGCCAAGTTGTCTATAAGCCGACCGATGGCTGGGGAGGGAACGGACTC
GTTAAGATAGAAGACGAAAGTGCACTCGATGTTTTAATCCCCTTTTTAAACCGGATGGGTTTGGAAAACCTTTATTTAGA
AAAATATATTAATTATGACAAAACAGATTGGCGAGTCGATATTGTTAATGGTGAATTTGTAGGCTGTTATGGCCGTAGCG
CGCCAGAAGGTGATTGGAAAACTAATATTACCAGCGGCGGTTCTATTTTATTACGGGAACCTAAAGATGATGTTATTGAG
TTAGCTATCAAAGCGGCAAAAGTCACTGGGCTTGAAATTGCAGGTGTTGATTTATTATATGATTTAGATACCGAAGAATA
TGTGGTGCTTGAAGTGAATGGGATACCCGCATTTGCAACACCAGAGCAAGAAAAAATAGGGCTAAATTTTAATGACTTTA
AAATTAATAAAATAGCCAATATGATAGAAAGTATTATAGAGGGTGAGCAGAGTGAAAAAAGCCACACAAAAATCGTTGCC
TAA

Upstream 100 bases:

>100_bases
TACTTGATATAAATGTATAGGTGGAGCGTTTTATTTAATCATAAAAATATCTAGCATTATTTATACAACGAGATGTACTT
CGAAACGGGGCAATCAAATA

Downstream 100 bases:

>100_bases
AATTGGTTTATTATATTTAGACCATGTCTTACGGTTTTTTGATAAATCAAATTTTAAAGGTTGGCCTGATAAAATAGAAA
GCGTTGTTTACCATTGGCGA

Product: RimK domain-containing protein ATP-grasp

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSSNKRIGSEPTVGIWLYKNGGGIEIQQKLIEILTKKGIRTITDLNLGHAYASGGEIICNGINMENLNAFFSYNAGEQST
FQLYMYQLLNQTTSTLNNYDAFALTEDKFLTAHALNHAGIRTAEYRMIHCDDISLLKKTVSDWQGQVVYKPTDGWGGNGL
VKIEDESALDVLIPFLNRMGLENLYLEKYINYDKTDWRVDIVNGEFVGCYGRSAPEGDWKTNITSGGSILLREPKDDVIE
LAIKAAKVTGLEIAGVDLLYDLDTEEYVVLEVNGIPAFATPEQEKIGLNFNDFKINKIANMIESIIEGEQSEKSHTKIVA

Sequences:

>Translated_320_residues
MSSNKRIGSEPTVGIWLYKNGGGIEIQQKLIEILTKKGIRTITDLNLGHAYASGGEIICNGINMENLNAFFSYNAGEQST
FQLYMYQLLNQTTSTLNNYDAFALTEDKFLTAHALNHAGIRTAEYRMIHCDDISLLKKTVSDWQGQVVYKPTDGWGGNGL
VKIEDESALDVLIPFLNRMGLENLYLEKYINYDKTDWRVDIVNGEFVGCYGRSAPEGDWKTNITSGGSILLREPKDDVIE
LAIKAAKVTGLEIAGVDLLYDLDTEEYVVLEVNGIPAFATPEQEKIGLNFNDFKINKIANMIESIIEGEQSEKSHTKIVA
>Mature_319_residues
SSNKRIGSEPTVGIWLYKNGGGIEIQQKLIEILTKKGIRTITDLNLGHAYASGGEIICNGINMENLNAFFSYNAGEQSTF
QLYMYQLLNQTTSTLNNYDAFALTEDKFLTAHALNHAGIRTAEYRMIHCDDISLLKKTVSDWQGQVVYKPTDGWGGNGLV
KIEDESALDVLIPFLNRMGLENLYLEKYINYDKTDWRVDIVNGEFVGCYGRSAPEGDWKTNITSGGSILLREPKDDVIEL
AIKAAKVTGLEIAGVDLLYDLDTEEYVVLEVNGIPAFATPEQEKIGLNFNDFKINKIANMIESIIEGEQSEKSHTKIVA

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=200, Percent_Identity=29.5, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 35652; Mature: 35521

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNKRIGSEPTVGIWLYKNGGGIEIQQKLIEILTKKGIRTITDLNLGHAYASGGEIICN
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEE
GINMENLNAFFSYNAGEQSTFQLYMYQLLNQTTSTLNNYDAFALTEDKFLTAHALNHAGI
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHCCCCC
RTAEYRMIHCDDISLLKKTVSDWQGQVVYKPTDGWGGNGLVKIEDESALDVLIPFLNRMG
EEEEEEEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCC
LENLYLEKYINYDKTDWRVDIVNGEFVGCYGRSAPEGDWKTNITSGGSILLREPKDDVIE
CHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEECCCCEEEEECCCHHHHH
LAIKAAKVTGLEIAGVDLLYDLDTEEYVVLEVNGIPAFATPEQEKIGLNFNDFKINKIAN
HHHHHHHCCCEEEECEEEEEECCCCCEEEEEECCCCCCCCCCHHHCCCCCCCEEHHHHHH
MIESIIEGEQSEKSHTKIVA
HHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure 
SSNKRIGSEPTVGIWLYKNGGGIEIQQKLIEILTKKGIRTITDLNLGHAYASGGEIICN
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEE
GINMENLNAFFSYNAGEQSTFQLYMYQLLNQTTSTLNNYDAFALTEDKFLTAHALNHAGI
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHCCCCC
RTAEYRMIHCDDISLLKKTVSDWQGQVVYKPTDGWGGNGLVKIEDESALDVLIPFLNRMG
EEEEEEEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCC
LENLYLEKYINYDKTDWRVDIVNGEFVGCYGRSAPEGDWKTNITSGGSILLREPKDDVIE
CHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCEECCCCEEEEECCCHHHHH
LAIKAAKVTGLEIAGVDLLYDLDTEEYVVLEVNGIPAFATPEQEKIGLNFNDFKINKIAN
HHHHHHHCCCEEEECEEEEEECCCCCEEEEEECCCCCCCCCCHHHCCCCCCCEEHHHHHH
MIESIIEGEQSEKSHTKIVA
HHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]