Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 119945892

GI number: 119945892

Start: 2715549

End: 2716232

Strand: Reverse

Name: phoB [H]

Synonym: Ping_2232

Alternate gene names: 119945892

Gene position: 2716232-2715549 (Counterclockwise)

Preceding gene: 119945897

Following gene: 119945891

Centisome position: 59.57

GC content: 42.54

Gene sequence:

>684_bases
ATGGCGAGCCGTATATTAGTTGTAGAAGATGAAGTCGCAATCCGCGATATGTTATGTTTTGTATTAGACCAGCATGGTTA
TGAGTGCATTGAAGCCGATACTTATCAAAGTGCATTAAATCAATTACACGAACCCTATCCCGATTTGATTTTACTGGATT
GGATGTTTCCGGGCGGTAGTGGCATAAAATTACTTAGCCACCTCAAACAAGATAATATTTTACGCTCCATCCCGGTCGTC
ATGTTGACCGCTCGAGGCGAAGAGCAAGATAAAGTCAGGGGCCTGGAAAATGGTGCGGATGATTATGTCACTAAACCCTT
TTCGCCTAAAGAGTTGATGGCGCGATTAAAAACAGTGATGCGACGTGTTAATCCAACTTCCCTCGATGATGAATTATCCT
TCAATAAGCTGAAACTCGATCCGGTTTCTCATCGTGTTTCGTTGGCCGATGAAGCATTGAATATGGGACCAACGGAGTTT
AATTTATTACATTTTTTTATGACTCATCCGGAACGCGTTTACAGTCGTGAGCAATTGCTTAATAATGTATGGGGAACCAA
TGTCTATGTTGAGGACCGTACCGTAGATGTACATATTAGACGACTGCGTAAAGCGTTAGCAGATCATGATCATTTAATCC
AAACCGTACGCGGTGCCGGTTATCGCTTTTCAGTCCGTAGCTAA

Upstream 100 bases:

>100_bases
AATGAAGGTTTGTAATATAACTGTCATAATGTAAGTCAATAATGGTTTAATCGAAGTAGTTTCAAACTAACCATTACTCA
GAACAGATAGGTAGTATCAA

Downstream 100 bases:

>100_bases
TATAACCTTAACCGTCATCTCAGGTGATGACGTATCGATTTGGAATGACTAAAGCTTCCACTTTAAGGGCCTAAACTATC
CACGGCTCACCGTGCTGATT

Product: two component transcriptional regulator, winged helix family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MASRILVVEDEVAIRDMLCFVLDQHGYECIEADTYQSALNQLHEPYPDLILLDWMFPGGSGIKLLSHLKQDNILRSIPVV
MLTARGEEQDKVRGLENGADDYVTKPFSPKELMARLKTVMRRVNPTSLDDELSFNKLKLDPVSHRVSLADEALNMGPTEF
NLLHFFMTHPERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALADHDHLIQTVRGAGYRFSVRS

Sequences:

>Translated_227_residues
MASRILVVEDEVAIRDMLCFVLDQHGYECIEADTYQSALNQLHEPYPDLILLDWMFPGGSGIKLLSHLKQDNILRSIPVV
MLTARGEEQDKVRGLENGADDYVTKPFSPKELMARLKTVMRRVNPTSLDDELSFNKLKLDPVSHRVSLADEALNMGPTEF
NLLHFFMTHPERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALADHDHLIQTVRGAGYRFSVRS
>Mature_226_residues
ASRILVVEDEVAIRDMLCFVLDQHGYECIEADTYQSALNQLHEPYPDLILLDWMFPGGSGIKLLSHLKQDNILRSIPVVM
LTARGEEQDKVRGLENGADDYVTKPFSPKELMARLKTVMRRVNPTSLDDELSFNKLKLDPVSHRVSLADEALNMGPTEFN
LLHFFMTHPERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALADHDHLIQTVRGAGYRFSVRS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=70.6140350877193, Blast_Score=350, Evalue=3e-98,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=39.2857142857143, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=37.6106194690265, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=34.061135371179, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=38.0090497737557, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1786784, Length=228, Percent_Identity=35.5263157894737, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=36.1607142857143, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.4978354978355, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.5545851528384, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=33.9449541284404, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790552, Length=218, Percent_Identity=33.4862385321101, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=32.4561403508772, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=30.7692307692308, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=27.8688524590164, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26089; Mature: 25958

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRILVVEDEVAIRDMLCFVLDQHGYECIEADTYQSALNQLHEPYPDLILLDWMFPGGS
CCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GIKLLSHLKQDNILRSIPVVMLTARGEEQDKVRGLENGADDYVTKPFSPKELMARLKTVM
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRVNPTSLDDELSFNKLKLDPVSHRVSLADEALNMGPTEFNLLHFFMTHPERVYSREQLL
HHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALADHDHLIQTVRGAGYRFSVRS
HHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
ASRILVVEDEVAIRDMLCFVLDQHGYECIEADTYQSALNQLHEPYPDLILLDWMFPGGS
CCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GIKLLSHLKQDNILRSIPVVMLTARGEEQDKVRGLENGADDYVTKPFSPKELMARLKTVM
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRVNPTSLDDELSFNKLKLDPVSHRVSLADEALNMGPTEFNLLHFFMTHPERVYSREQLL
HHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALADHDHLIQTVRGAGYRFSVRS
HHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]