Click here to switch to the map view.

The map label for this gene is opuD [H]

Identifier: 119945790

GI number: 119945790

Start: 2591049

End: 2592650

Strand: Reverse

Name: opuD [H]

Synonym: Ping_2126

Alternate gene names: 119945790

Gene position: 2592650-2591049 (Counterclockwise)

Preceding gene: 119945791

Following gene: 119945789

Centisome position: 56.86

GC content: 46.57

Gene sequence:

>1602_bases
ATGTCTGATTTAATCAATAGAGTGAAAGCTTCTAGCTTTGAAGCTTCGGAAATCAATACTGCACAATCTGCAAGTATAAA
TAACAGCAAAAGTATTGCCGACAAACTTGGGTTGGATAACCCTGCTTTTTGGTACAGCGGCGGATTTATCAGCCTGTTCG
TTCTGCTCGCACTATACGATGCTGAGCTGCTGTCGAGCCTGGTGAATACTGGCTTCTCATGGTCAGTTAAAGTCTTTGGC
CCATATTGGCAATTACTGCTGCTGCTGACTTTTATTATCGGTATTGCGCTGGCAGCCGGACGAACAGGCAAGGTTATTCT
TGGCGGCATAGCCAAGCCTGAAATGGATGGGTTCCGTTGGATGGCGATTATCTTTTGTACATTACTTGCAGGGGGTGGTG
TATTTTGGGCGGCAGCGGAACCCATCGCGCATTTTGTGAGTCCTCCTCCGCTTTATGGTCCTCAGCTGGATGCTCAGCAA
GGTGCGGTGAATGCGTTGTCACAATCCTTTATGCACTGGGGGTTTTTGGCTTGGGCAATCGTTGGCAGCTTAACGTCGAT
TGTGGTGATGCACTTACATTACGACAAAGGTCTGCCGCTAAAGCCGCGTATTTTACTTTACCCGGTGTTAGGCGAGCGGG
TGCTTAAAGGTCACACTGGCGCGTTGATTGATGCATGTTGTATCGTTGCGGTGGCGGCGGGTACGATAGGTCCGATCGGC
TTTTTAGGCTTGCAAGTTAGCTACGCATTAAATGCTCTTTTTGATATCCCGGATGGTTTTACCACTCAGTTAATCATTAT
TCTGTTTGCCATTGTACTATACACTTTATCAGCACTTAGTGGTTTGAACCGCGGCATGCAAATGTTAAGTCGGTATAACG
TTATTTTAGCGATGATACTGATGGTTTACATTCTGATTTTTGGGCCAACGAATTTTATCTTTAATGGTTACATTCAAGGC
GTAGGCAGCATGTTGGATAACTTTATTCCAATGGCGACTTACCGCGGAGATGAAGGTTGGTTGAGCTGGTGGACAGTCTT
CTTCTGGGGTTGGTTCCTGGGCTACGGTCCAATGATGGCTATCTTTATTGCCCGTATCTCCCGTGGCCGCAGCATTCGCC
AATTGGTTACGACCATTAGTTTGATAGCACCACTTGTGACCTGCTTCTGGTTTACCATAGTTGGAGGCTCTGGTTTGGCA
TTTGAAATTGCGAATCCGGGCAGTGTGAGTAGCGCATTTGAAGGTTTCAACTTACCTGGGGCACTGCTGGCGGTGACTCA
GCAGCTACCACTACCTATGCTTGTTTCAATCTTATTCCTGATCCTGACCACTATCTTCATCGTGACAACAGGTGACTCGA
TGACTTACACCATCAGTGTGGTTATTAGTGGTGAAACTGAACCAAATGCAATGATTCGAATCTTTTGGGGAGTTATGATG
GGGGTAACTGCTTTGATACTGATTTCCCTTGGTTCTGGTGGTATCTCTGCGCTGCAATCATTCATTGTTATCACAGCTGT
ACCCGTTTCTATTATCTTACTGCCATCACTCTGGAACGCGCCGCAAATCGCGATCCAGATGGCGAAAGATCAAGGGCTAT
AA

Upstream 100 bases:

>100_bases
GGAACAGTCTGCCCAATATGAAAGTAATAGACAGCTGTTAAATATAACTCTTGTAGTCATCATCTATATGGCAGCTGCAA
AGAACCACTGGAGTCAGAAT

Downstream 100 bases:

>100_bases
AACGCATTGGTGTCAATACATCAGTCGAAGTAATGCTCAGGGGGCGAGTTCGCTCGCCCTAACTGGAAGATCAAATACTT
TAATAACTAAAATGGTGGTG

Product: BCCT transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSDLINRVKASSFEASEINTAQSASINNSKSIADKLGLDNPAFWYSGGFISLFVLLALYDAELLSSLVNTGFSWSVKVFG
PYWQLLLLLTFIIGIALAAGRTGKVILGGIAKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSPPPLYGPQLDAQQ
GAVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERVLKGHTGALIDACCIVAVAAGTIGPIG
FLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTLSALSGLNRGMQMLSRYNVILAMILMVYILIFGPTNFIFNGYIQG
VGSMLDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLVTTISLIAPLVTCFWFTIVGGSGLA
FEIANPGSVSSAFEGFNLPGALLAVTQQLPLPMLVSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAMIRIFWGVMM
GVTALILISLGSGGISALQSFIVITAVPVSIILLPSLWNAPQIAIQMAKDQGL

Sequences:

>Translated_533_residues
MSDLINRVKASSFEASEINTAQSASINNSKSIADKLGLDNPAFWYSGGFISLFVLLALYDAELLSSLVNTGFSWSVKVFG
PYWQLLLLLTFIIGIALAAGRTGKVILGGIAKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSPPPLYGPQLDAQQ
GAVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERVLKGHTGALIDACCIVAVAAGTIGPIG
FLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTLSALSGLNRGMQMLSRYNVILAMILMVYILIFGPTNFIFNGYIQG
VGSMLDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLVTTISLIAPLVTCFWFTIVGGSGLA
FEIANPGSVSSAFEGFNLPGALLAVTQQLPLPMLVSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAMIRIFWGVMM
GVTALILISLGSGGISALQSFIVITAVPVSIILLPSLWNAPQIAIQMAKDQGL
>Mature_532_residues
SDLINRVKASSFEASEINTAQSASINNSKSIADKLGLDNPAFWYSGGFISLFVLLALYDAELLSSLVNTGFSWSVKVFGP
YWQLLLLLTFIIGIALAAGRTGKVILGGIAKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSPPPLYGPQLDAQQG
AVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERVLKGHTGALIDACCIVAVAAGTIGPIGF
LGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTLSALSGLNRGMQMLSRYNVILAMILMVYILIFGPTNFIFNGYIQGV
GSMLDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLVTTISLIAPLVTCFWFTIVGGSGLAF
EIANPGSVSSAFEGFNLPGALLAVTQQLPLPMLVSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAMIRIFWGVMMG
VTALILISLGSGGISALQSFIVITAVPVSIILLPSLWNAPQIAIQMAKDQGL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=490, Percent_Identity=29.5918367346939, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1788102, Length=436, Percent_Identity=28.2110091743119, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786224, Length=488, Percent_Identity=26.2295081967213, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57516; Mature: 57385

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLINRVKASSFEASEINTAQSASINNSKSIADKLGLDNPAFWYSGGFISLFVLLALYD
CCHHHHHHHCCCCCHHHCCCHHHCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHH
AELLSSLVNTGFSWSVKVFGPYWQLLLLLTFIIGIALAAGRTGKVILGGIAKPEMDGFRW
HHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHH
MAIIFCTLLAGGGVFWAAAEPIAHFVSPPPLYGPQLDAQQGAVNALSQSFMHWGFLAWAI
HHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERVLKGHTGALIDACCIVAVAAGTIGPIG
HHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTLSALSGLNRGMQMLSRYNVILAMIL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVYILIFGPTNFIFNGYIQGVGSMLDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IFIARISRGRSIRQLVTTISLIAPLVTCFWFTIVGGSGLAFEIANPGSVSSAFEGFNLPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCH
ALLAVTQQLPLPMLVSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAMIRIFWGVMM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHH
GVTALILISLGSGGISALQSFIVITAVPVSIILLPSLWNAPQIAIQMAKDQGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCC
>Mature Secondary Structure 
SDLINRVKASSFEASEINTAQSASINNSKSIADKLGLDNPAFWYSGGFISLFVLLALYD
CHHHHHHHCCCCCHHHCCCHHHCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHH
AELLSSLVNTGFSWSVKVFGPYWQLLLLLTFIIGIALAAGRTGKVILGGIAKPEMDGFRW
HHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHH
MAIIFCTLLAGGGVFWAAAEPIAHFVSPPPLYGPQLDAQQGAVNALSQSFMHWGFLAWAI
HHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERVLKGHTGALIDACCIVAVAAGTIGPIG
HHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTLSALSGLNRGMQMLSRYNVILAMIL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVYILIFGPTNFIFNGYIQGVGSMLDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IFIARISRGRSIRQLVTTISLIAPLVTCFWFTIVGGSGLAFEIANPGSVSSAFEGFNLPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCH
ALLAVTQQLPLPMLVSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAMIRIFWGVMM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHH
GVTALILISLGSGGISALQSFIVITAVPVSIILLPSLWNAPQIAIQMAKDQGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]