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The map label for this gene is bcr [H]

Identifier: 119945494

GI number: 119945494

Start: 2181466

End: 2182653

Strand: Direct

Name: bcr [H]

Synonym: Ping_1796

Alternate gene names: 119945494

Gene position: 2181466-2182653 (Clockwise)

Preceding gene: 119945493

Following gene: 119945496

Centisome position: 47.84

GC content: 41.5

Gene sequence:

>1188_bases
ATGGATTCGTCCTCCACAACAAAATTAAGTTTTTTACTGATCGTTATTCTGGGTGGTATCACCTCCCTTACTCCTTTTGC
TATTGATATGTACCTGCCAGCAATGCCGAGTATTGCAAAAGAGTTTGGGGTAAGTGCAGGTGCCATTCAAATTACCTTGA
CCTCTTTTATTGCCGGATTTGCAGTTGGCCAGCTATTTCATGGTCCTTTATCAGATAGTTTTGGTCGTAAGCCTGTGTTG
CTGCTTGGTACCTTTTTCTTCGCGCTTGCCGCTGTTATTAGTGCAGCGAGCGACAGTATTGACGCGCTTATGTGGGCACG
TGCTGCGCAAGGTTTTTCAGGTGCTGCTGGTGCTGTTGTTGTTCAAGCTATTGTTAGAGATATGTTTGCCAAGGAAGATT
TTGCTCGCACTATGTCATTTATTATATTAGTGATGACTATTGCTCCCTTATTAGCGCCACTTGCTGGTGGTTATCTGGCC
GTCTGGTTTGGTTGGCGCTCTATCTTTTGGTTATTAGCCCTGATTGCATTTGTTATGATTATCGCTGCCAGCTTAGTTAT
TCCTGAAACCTTAAAAGCTGAAAATAGACAACCTTTTCGCCTGCGCACTACAATGCGCCATTTTTTTACCCTCTTTACCC
GATCCAAAGCCTTTGGATTGATTTTTACCGGGGCTTTTTCATTTGCTGGTATGTTTGCATTTTTAACGGCTGGCTCTTTC
ATATACATAGAAATATATGGTGTGAGCATCACTAATGTTGGTTATTTATTTGGCTTAAATATACTTTTAATGATGGTGAT
GACGACATTAAACGGAAAATATGTAAAAAGATTAGGATCGCATTATCTGCTTCGTTTGGGGTTAACTATTCAACTAATCG
CAAGCCTGCTCTTAGTGTTAGCGCAGTTATTAGATTTAGGTTTATGGGGAGTTGTTGTACCTGTTATGTTATTTTTCAGC
ACTATTTCAATGGTGGGCAGTAATAGTATCGCGTTATTATTAAACGAATATCCGGAAATAGCGGGTACGGTATCTTCGTT
GGCGGGCTCCCTCAAATTTGGTGCGGGTGCCTTGGTCGCTGCTGCTGTCTCTATGTTACCTAGTGGCAGTGTTTGGCCGA
TGATATTAATGATGGTGTTATGCAGTTTGCTGTCAATGACATTTTATACTTACTGTGCAAAGAGGTAA

Upstream 100 bases:

>100_bases
TAAAAGTGAAAATTCTGTGTGTCAAGTGTAAAAAAATAATAAACGAGAGCTGGTTCATGTTATTCTAACTTTTTTATTTC
CTTTTATCCCAGAGAAGATT

Downstream 100 bases:

>100_bases
AATTAAAGCCGTGAGCAATGAGTTAAAAGCCAGACTGCGATGCTCGATATTATGACATTAGTGAAAGCTGACTTTCCTTT
TGATTATCCCAATATTCATA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MDSSSTTKLSFLLIVILGGITSLTPFAIDMYLPAMPSIAKEFGVSAGAIQITLTSFIAGFAVGQLFHGPLSDSFGRKPVL
LLGTFFFALAAVISAASDSIDALMWARAAQGFSGAAGAVVVQAIVRDMFAKEDFARTMSFIILVMTIAPLLAPLAGGYLA
VWFGWRSIFWLLALIAFVMIIAASLVIPETLKAENRQPFRLRTTMRHFFTLFTRSKAFGLIFTGAFSFAGMFAFLTAGSF
IYIEIYGVSITNVGYLFGLNILLMMVMTTLNGKYVKRLGSHYLLRLGLTIQLIASLLLVLAQLLDLGLWGVVVPVMLFFS
TISMVGSNSIALLLNEYPEIAGTVSSLAGSLKFGAGALVAAAVSMLPSGSVWPMILMMVLCSLLSMTFYTYCAKR

Sequences:

>Translated_395_residues
MDSSSTTKLSFLLIVILGGITSLTPFAIDMYLPAMPSIAKEFGVSAGAIQITLTSFIAGFAVGQLFHGPLSDSFGRKPVL
LLGTFFFALAAVISAASDSIDALMWARAAQGFSGAAGAVVVQAIVRDMFAKEDFARTMSFIILVMTIAPLLAPLAGGYLA
VWFGWRSIFWLLALIAFVMIIAASLVIPETLKAENRQPFRLRTTMRHFFTLFTRSKAFGLIFTGAFSFAGMFAFLTAGSF
IYIEIYGVSITNVGYLFGLNILLMMVMTTLNGKYVKRLGSHYLLRLGLTIQLIASLLLVLAQLLDLGLWGVVVPVMLFFS
TISMVGSNSIALLLNEYPEIAGTVSSLAGSLKFGAGALVAAAVSMLPSGSVWPMILMMVLCSLLSMTFYTYCAKR
>Mature_395_residues
MDSSSTTKLSFLLIVILGGITSLTPFAIDMYLPAMPSIAKEFGVSAGAIQITLTSFIAGFAVGQLFHGPLSDSFGRKPVL
LLGTFFFALAAVISAASDSIDALMWARAAQGFSGAAGAVVVQAIVRDMFAKEDFARTMSFIILVMTIAPLLAPLAGGYLA
VWFGWRSIFWLLALIAFVMIIAASLVIPETLKAENRQPFRLRTTMRHFFTLFTRSKAFGLIFTGAFSFAGMFAFLTAGSF
IYIEIYGVSITNVGYLFGLNILLMMVMTTLNGKYVKRLGSHYLLRLGLTIQLIASLLLVLAQLLDLGLWGVVVPVMLFFS
TISMVGSNSIALLLNEYPEIAGTVSSLAGSLKFGAGALVAAAVSMLPSGSVWPMILMMVLCSLLSMTFYTYCAKR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=391, Percent_Identity=47.3145780051151, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI48994889, Length=245, Percent_Identity=36.3265306122449, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87082312, Length=345, Percent_Identity=28.4057971014493, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1790146, Length=225, Percent_Identity=27.5555555555556, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790794, Length=286, Percent_Identity=26.2237762237762, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787065, Length=162, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787880, Length=281, Percent_Identity=27.4021352313167, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081738, Length=175, Percent_Identity=32.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=171, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=167, Percent_Identity=24.5508982035928, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=157, Percent_Identity=31.2101910828025, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=174, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42505; Mature: 42505

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]