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The map label for this gene is paaJ [H]

Identifier: 119944431

GI number: 119944431

Start: 844194

End: 845399

Strand: Direct

Name: paaJ [H]

Synonym: Ping_0659

Alternate gene names: 119944431

Gene position: 844194-845399 (Clockwise)

Preceding gene: 119944430

Following gene: 119944432

Centisome position: 18.51

GC content: 44.2

Gene sequence:

>1206_bases
ATGATTAATGCATATATCTGCGATGCAATAAGAACACCAATAGGTCGTTACGGCGGCGGTCTCTCACCAATACGTCCGGA
TGATCTGGGCGCTTTACCCATTAAAGCCATTATGGAACGTAATCCGGATATTGATTGGCGAGAAATTGATGATGTTATTT
ATGGGTGTGCAAACCAGGCTGGAGAAGATAATCGTAATGTTGCGCGCATGAGTCTGCTGCTTGCCGGATTGCCGGAAGAA
GTATCAGGTACCACTATTAATCGCTTATGTGGCTCTGGTATGGATGCTATTTCACTTGTTGCAAGAAGTATTAAGGCAGG
TGATGCAGAGCTTATGATTGCTGGTGGTGTTGAATCAATGTCACGCGCTCCTTTTGTGATGGGTAAAGCGACGTCAGCTT
ACTCCCGCAATGCAGAAATGTACGATACAACCATTGGATGGCGCTTTATAAATAAAGAGCTGCAAAAACAGTTTGGCACG
GACTCTATGCCTGAAACAGCAGAAAATGTCGCTGCCCGATACAATATCTCCCGCGAAGATCAGGATGCTTTTGCCCTAAG
AAGTCAATACCGCACTGAACAAGCCCAGAAATCGGGAAGACTGTCAGAAGAAATTGTGCCGGTGACCATTTTAAATCGCA
AATCTGAACCAACCATTATTGATAAAGATGAGCACCCGCGTCCAGAAACAACGCTTTCTCAATTAGCTAATTTACGTACT
CCTTTTGCTGAAAATGGCAGTGTGACTGCTGGCAATTCGTCTGGTGTTAATGATGGTGCTGCTGCAATGTTAATCGCAAG
CGACAAAGCGGTTATTAAATACGGTTTATTACCAAAAGCAAAAGTGATTGCCTGTGCAACTGCCGGGGTTGAGCCATCCA
TAATGGGAATGGGTCCAGCACCTGCGGTTAAAAAAGTGCTTAAAAAAGCGGGTTTATCAATTGATGATATGGATGTAATT
GAATTAAATGAAGCTTTTGCAGCACAATCATTAGCCGTATTACGTGAATTAGGGTTGCCTGATGATGCTGATCATGTCAA
CCCAAATGGCGGTGCCATTGCATTAGGGCATCCACTGGGAATGAGCGGTACTCGACTTGTTATGGCTGCCACTAATGAGT
TAACACGACGTAAAGGAAAATATGCGCTTTGTTGCATGTGTATCGGTGTTGGTCAAGGCATTGCCATGATCATTGAACGC
GTTTAA

Upstream 100 bases:

>100_bases
ACCTATGATGTTGAAGTCACCAATCAACAGCAAAAATTGGTCGCTCTATTTAGAGGGAAAGCGTATCGGTTAGGCACTCA
AATATTAGGAGATAACAACA

Downstream 100 bases:

>100_bases
TTGCTTATTTAACTCTGTTGTTAGGGCTATAAAAACAGAGTGATATATTAAAAAGGAAAGAGAACAATGACAATTTATAA
TAAATTAGAATCAATTGAAA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MINAYICDAIRTPIGRYGGGLSPIRPDDLGALPIKAIMERNPDIDWREIDDVIYGCANQAGEDNRNVARMSLLLAGLPEE
VSGTTINRLCGSGMDAISLVARSIKAGDAELMIAGGVESMSRAPFVMGKATSAYSRNAEMYDTTIGWRFINKELQKQFGT
DSMPETAENVAARYNISREDQDAFALRSQYRTEQAQKSGRLSEEIVPVTILNRKSEPTIIDKDEHPRPETTLSQLANLRT
PFAENGSVTAGNSSGVNDGAAAMLIASDKAVIKYGLLPKAKVIACATAGVEPSIMGMGPAPAVKKVLKKAGLSIDDMDVI
ELNEAFAAQSLAVLRELGLPDDADHVNPNGGAIALGHPLGMSGTRLVMAATNELTRRKGKYALCCMCIGVGQGIAMIIER
V

Sequences:

>Translated_401_residues
MINAYICDAIRTPIGRYGGGLSPIRPDDLGALPIKAIMERNPDIDWREIDDVIYGCANQAGEDNRNVARMSLLLAGLPEE
VSGTTINRLCGSGMDAISLVARSIKAGDAELMIAGGVESMSRAPFVMGKATSAYSRNAEMYDTTIGWRFINKELQKQFGT
DSMPETAENVAARYNISREDQDAFALRSQYRTEQAQKSGRLSEEIVPVTILNRKSEPTIIDKDEHPRPETTLSQLANLRT
PFAENGSVTAGNSSGVNDGAAAMLIASDKAVIKYGLLPKAKVIACATAGVEPSIMGMGPAPAVKKVLKKAGLSIDDMDVI
ELNEAFAAQSLAVLRELGLPDDADHVNPNGGAIALGHPLGMSGTRLVMAATNELTRRKGKYALCCMCIGVGQGIAMIIER
V
>Mature_401_residues
MINAYICDAIRTPIGRYGGGLSPIRPDDLGALPIKAIMERNPDIDWREIDDVIYGCANQAGEDNRNVARMSLLLAGLPEE
VSGTTINRLCGSGMDAISLVARSIKAGDAELMIAGGVESMSRAPFVMGKATSAYSRNAEMYDTTIGWRFINKELQKQFGT
DSMPETAENVAARYNISREDQDAFALRSQYRTEQAQKSGRLSEEIVPVTILNRKSEPTIIDKDEHPRPETTLSQLANLRT
PFAENGSVTAGNSSGVNDGAAAMLIASDKAVIKYGLLPKAKVIACATAGVEPSIMGMGPAPAVKKVLKKAGLSIDDMDVI
ELNEAFAAQSLAVLRELGLPDDADHVNPNGGAIALGHPLGMSGTRLVMAATNELTRRKGKYALCCMCIGVGQGIAMIIER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=43.5443037974684, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=40.2010050251256, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=36.7758186397985, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=40.3990024937656, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=32.8671328671329, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI194353979, Length=248, Percent_Identity=31.0483870967742, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=71.3216957605985, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.1612903225806, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=44.3069306930693, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.1471321695761, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=30.3738317757009, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=42.1052631578947, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25147385, Length=401, Percent_Identity=34.4139650872818, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=34.75, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=439, Percent_Identity=31.2072892938497, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=406, Percent_Identity=28.0788177339901, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=37.3464373464373, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322031, Length=398, Percent_Identity=35.678391959799, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.6384039900249, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=41.5841584158416, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24640423, Length=400, Percent_Identity=35.5, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=31.9347319347319, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42815; Mature: 42815

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINAYICDAIRTPIGRYGGGLSPIRPDDLGALPIKAIMERNPDIDWREIDDVIYGCANQA
CCCCEEHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSLLLAGLPEEVSGTTINRLCGSGMDAISLVARSIKAGDAELMIAGGVESM
CCCCCHHHHHHHHHHCCCCHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPFVMGKATSAYSRNAEMYDTTIGWRFINKELQKQFGTDSMPETAENVAARYNISRED
HCCCEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQYRTEQAQKSGRLSEEIVPVTILNRKSEPTIIDKDEHPRPETTLSQLANLRT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHCC
PFAENGSVTAGNSSGVNDGAAAMLIASDKAVIKYGLLPKAKVIACATAGVEPSIMGMGPA
CCCCCCCEECCCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCCCCCEECCCCC
PAVKKVLKKAGLSIDDMDVIELNEAFAAQSLAVLRELGLPDDADHVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGTRLVMAATNELTRRKGKYALCCMCIGVGQGIAMIIERV
CCCCEEEEEEHHHHHHHCCCEEEEEEEHHHCCCHHHHHHCC
>Mature Secondary Structure
MINAYICDAIRTPIGRYGGGLSPIRPDDLGALPIKAIMERNPDIDWREIDDVIYGCANQA
CCCCEEHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSLLLAGLPEEVSGTTINRLCGSGMDAISLVARSIKAGDAELMIAGGVESM
CCCCCHHHHHHHHHHCCCCHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPFVMGKATSAYSRNAEMYDTTIGWRFINKELQKQFGTDSMPETAENVAARYNISRED
HCCCEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQYRTEQAQKSGRLSEEIVPVTILNRKSEPTIIDKDEHPRPETTLSQLANLRT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHCC
PFAENGSVTAGNSSGVNDGAAAMLIASDKAVIKYGLLPKAKVIACATAGVEPSIMGMGPA
CCCCCCCEECCCCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCCCCCEECCCCC
PAVKKVLKKAGLSIDDMDVIELNEAFAAQSLAVLRELGLPDDADHVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGTRLVMAATNELTRRKGKYALCCMCIGVGQGIAMIIERV
CCCCEEEEEEHHHHHHHCCCEEEEEEEHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]