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The map label for this gene is paaI [H]

Identifier: 119944430

GI number: 119944430

Start: 843761

End: 844201

Strand: Direct

Name: paaI [H]

Synonym: Ping_0658

Alternate gene names: 119944430

Gene position: 843761-844201 (Clockwise)

Preceding gene: 119944429

Following gene: 119944431

Centisome position: 18.51

GC content: 41.72

Gene sequence:

>441_bases
ATGGAGAATAGTCAAGAATTAGCATGGCGTTGTGCCAAAAAAATGTATAACAATGATGCTTGCTCTAATTTCTTAGGAAT
GAAAATCGAAGACATGGGTAAGGGATATGCGGTGCTTAATATGGTTGTCAGTAACACTATGTTAAACGGTTTTCCAAGTT
GCCATGGCGGAATGATTTTTTCCCTTGCAGATTCAGCCTTTGCCTTTGCATGTAACAGTGAGAATCAGACTGCTGTGGCT
GCGGGTTGCAATATTGAATATCTTCGCCCGGGGTTTGAGGGCGACATACTTACGGCAACGGCGCATATGAAATCCCAGGG
AAAAGTAACCGGTACCTATGATGTTGAAGTCACCAATCAACAGCAAAAATTGGTCGCTCTATTTAGAGGGAAAGCGTATC
GGTTAGGCACTCAAATATTAGGAGATAACAACAATGATTAA

Upstream 100 bases:

>100_bases
AAAAACGTCAAAGAAACATTGGAGTCTTTAACGGCTTTTTATGGTGAAGAGCGCTACCGGCCATCCCCCTTATTAAGACA
ATTATCTTACGGAGTCATTA

Downstream 100 bases:

>100_bases
TGCATATATCTGCGATGCAATAAGAACACCAATAGGTCGTTACGGCGGCGGTCTCTCACCAATACGTCCGGATGATCTGG
GCGCTTTACCCATTAAAGCC

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MENSQELAWRCAKKMYNNDACSNFLGMKIEDMGKGYAVLNMVVSNTMLNGFPSCHGGMIFSLADSAFAFACNSENQTAVA
AGCNIEYLRPGFEGDILTATAHMKSQGKVTGTYDVEVTNQQQKLVALFRGKAYRLGTQILGDNNND

Sequences:

>Translated_146_residues
MENSQELAWRCAKKMYNNDACSNFLGMKIEDMGKGYAVLNMVVSNTMLNGFPSCHGGMIFSLADSAFAFACNSENQTAVA
AGCNIEYLRPGFEGDILTATAHMKSQGKVTGTYDVEVTNQQQKLVALFRGKAYRLGTQILGDNNND
>Mature_146_residues
MENSQELAWRCAKKMYNNDACSNFLGMKIEDMGKGYAVLNMVVSNTMLNGFPSCHGGMIFSLADSAFAFACNSENQTAVA
AGCNIEYLRPGFEGDILTATAHMKSQGKVTGTYDVEVTNQQQKLVALFRGKAYRLGTQILGDNNND

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=135, Percent_Identity=59.2592592592593, Blast_Score=177, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15912; Mature: 15912

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSQELAWRCAKKMYNNDACSNFLGMKIEDMGKGYAVLNMVVSNTMLNGFPSCHGGMIF
CCCHHHHHHHHHHHHHCCCCHHHHCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
SLADSAFAFACNSENQTAVAAGCNIEYLRPGFEGDILTATAHMKSQGKVTGTYDVEVTNQ
EECCCEEEEEECCCCCEEEEECCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCC
QQKLVALFRGKAYRLGTQILGDNNND
HHHHHHHHCCCCEEECEEEECCCCCC
>Mature Secondary Structure
MENSQELAWRCAKKMYNNDACSNFLGMKIEDMGKGYAVLNMVVSNTMLNGFPSCHGGMIF
CCCHHHHHHHHHHHHHCCCCHHHHCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
SLADSAFAFACNSENQTAVAAGCNIEYLRPGFEGDILTATAHMKSQGKVTGTYDVEVTNQ
EECCCEEEEEECCCCCEEEEECCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCC
QQKLVALFRGKAYRLGTQILGDNNND
HHHHHHHHCCCCEEECEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]