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The map label for this gene is miaB

Identifier: 119944308

GI number: 119944308

Start: 683291

End: 684712

Strand: Reverse

Name: miaB

Synonym: Ping_0535

Alternate gene names: 119944308

Gene position: 684712-683291 (Counterclockwise)

Preceding gene: 119944310

Following gene: 119944307

Centisome position: 15.02

GC content: 43.46

Gene sequence:

>1422_bases
ATGAGCATGAAATTACACGTCAAAACTTGGGGTTGTCAAATGAATGAGTATGACTCATCAAAAATGGCCGACCTACTAAA
TTCAACAAACGGTTATATCTCAGTTGAGCATGCAGAAGATGCGGACGTGGTGTTATTAAATACTTGTTCTATCCGTGAAA
AAGCCCAGGAAAAGGTATTCCATCAGCTGGGCCGCTGGAAAAAATTAAAGGTTAACAAACCCAATCTGGTGATTGGTGTG
GGTGGTTGTGTTGCCTCCCAGGAAGGTAAAGCGATTCGCAGTCGTGCCCCTTTTGTCGATATTGTTTTTGGCCCGCAAAC
CTTACACCGTTTACCCGAAATGATAAAAGAAGTACAAGAAAACAAAGGCACTGTGGTTGATGTTAGCTTCCCGGAAATTG
AAAAATTTGACTCATTACCTGAGCCGAAAGCCGATGGTGCAACCGCCTTTGTCTCGATTATGGAAGGCTGTTCAAAATAT
TGTTCTTTCTGCGTTGTACCCTATACCCGGGGTGAAGAAGTTAGCCGCCCACTCGATGATGTGTTATTAGAAGTTGCCCA
GTTAGCTGAACAGGGTGTGCGTGAAGTAACCCTGTTAGGTCAAAATGTTAATTCTTATCTTGGTGCAACCTATGACGGTG
ATACCTGCAGTTTTGCTGAACTGTTACGTTTTGTTGCATCCATTGATGGTATTGATCGCCTGCGCTACGTCACCAGTAAT
CCGATTGATTTTAGCGATGAAATTATCGCCGTCTATGAAGATACACCGGAACTGGTCAATTTCTTACATTTACCCGTACA
AGCGGGTTCGGATAGAATACTAGCGGCAATGAAACGGGGTCATACCGTTGCCCAGTACAAAGATCAAATAGCGCGTTTAT
TACAAGTCCGCCCGGAACTCACCGTCAGCTCCGATTTTATTATTGGCTTCCCGAATGAAACCGATCACGATTTTGAGCAA
ACCATGGATCTGATTAAATATATTAATTTTGATACCAGCTATAGCTTTATTTATAGTCAGCGTCCGGGCACACCAGCGGC
TGATATGCCCGATGATGTGACTCTGGAAACTAAAAAACAGCGCCTGGCAATTCTGCAGGACAGAATTCAGCAGCAATCGC
AGGGCATTGGCCGGAAAATGGTGGGCAGCGTTCAGCGTATTTTAGTTGAAGGACCTTCACGTAAAAATATTATGGAATTA
TGCGGTCGTACTGAAAACAACCGTATTGTTAACTTTGAGGGTGACCATCGTTCCATTGGCGGCTTTGTTGATGTAGAAAT
AACGGAAGTTCATACTAACTCAATCCGTGGGAAATTCATCCGTGGAGAAGCAGAAATGAACCTGCGTGAATCAATTCGCC
CGAGTGATATTGTTAACAAACATGAAAACAAATCAGCTGTTAACAGCTATATTCCTGCCTAA

Upstream 100 bases:

>100_bases
ATTAATCTTAAAAAAGGAAAAAAACAAAGAGTAGCATGATCGACCTGATGTGCGTAAAATACGACCTCTTTTTTAGATCC
ATTGTATGAGCTAATATATT

Downstream 100 bases:

>100_bases
TTGGCTTAATCAACAAAACTAACCCTATTAATAGGTTAGTTTTGTTGATATAAAGTTTATCAAGATTAATGCTTTCAAAA
GCTCCCTCAGGGGACTCAGA

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSMKLHVKTWGCQMNEYDSSKMADLLNSTNGYISVEHAEDADVVLLNTCSIREKAQEKVFHQLGRWKKLKVNKPNLVIGV
GGCVASQEGKAIRSRAPFVDIVFGPQTLHRLPEMIKEVQENKGTVVDVSFPEIEKFDSLPEPKADGATAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVLLEVAQLAEQGVREVTLLGQNVNSYLGATYDGDTCSFAELLRFVASIDGIDRLRYVTSN
PIDFSDEIIAVYEDTPELVNFLHLPVQAGSDRILAAMKRGHTVAQYKDQIARLLQVRPELTVSSDFIIGFPNETDHDFEQ
TMDLIKYINFDTSYSFIYSQRPGTPAADMPDDVTLETKKQRLAILQDRIQQQSQGIGRKMVGSVQRILVEGPSRKNIMEL
CGRTENNRIVNFEGDHRSIGGFVDVEITEVHTNSIRGKFIRGEAEMNLRESIRPSDIVNKHENKSAVNSYIPA

Sequences:

>Translated_473_residues
MSMKLHVKTWGCQMNEYDSSKMADLLNSTNGYISVEHAEDADVVLLNTCSIREKAQEKVFHQLGRWKKLKVNKPNLVIGV
GGCVASQEGKAIRSRAPFVDIVFGPQTLHRLPEMIKEVQENKGTVVDVSFPEIEKFDSLPEPKADGATAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVLLEVAQLAEQGVREVTLLGQNVNSYLGATYDGDTCSFAELLRFVASIDGIDRLRYVTSN
PIDFSDEIIAVYEDTPELVNFLHLPVQAGSDRILAAMKRGHTVAQYKDQIARLLQVRPELTVSSDFIIGFPNETDHDFEQ
TMDLIKYINFDTSYSFIYSQRPGTPAADMPDDVTLETKKQRLAILQDRIQQQSQGIGRKMVGSVQRILVEGPSRKNIMEL
CGRTENNRIVNFEGDHRSIGGFVDVEITEVHTNSIRGKFIRGEAEMNLRESIRPSDIVNKHENKSAVNSYIPA
>Mature_472_residues
SMKLHVKTWGCQMNEYDSSKMADLLNSTNGYISVEHAEDADVVLLNTCSIREKAQEKVFHQLGRWKKLKVNKPNLVIGVG
GCVASQEGKAIRSRAPFVDIVFGPQTLHRLPEMIKEVQENKGTVVDVSFPEIEKFDSLPEPKADGATAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPLDDVLLEVAQLAEQGVREVTLLGQNVNSYLGATYDGDTCSFAELLRFVASIDGIDRLRYVTSNP
IDFSDEIIAVYEDTPELVNFLHLPVQAGSDRILAAMKRGHTVAQYKDQIARLLQVRPELTVSSDFIIGFPNETDHDFEQT
MDLIKYINFDTSYSFIYSQRPGTPAADMPDDVTLETKKQRLAILQDRIQQQSQGIGRKMVGSVQRILVEGPSRKNIMELC
GRTENNRIVNFEGDHRSIGGFVDVEITEVHTNSIRGKFIRGEAEMNLRESIRPSDIVNKHENKSAVNSYIPA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=35.2201257861635, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=35.2201257861635, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=29.7229219143577, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=68.1434599156118, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=427, Percent_Identity=29.2740046838407, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=31.8872017353579, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=366, Percent_Identity=26.2295081967213, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=34.1772151898734, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=28.0434782608696, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PSYIN (A1SSC4)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941988.1
- ProteinModelPortal:   A1SSC4
- SMR:   A1SSC4
- STRING:   A1SSC4
- GeneID:   4624847
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0535
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A1SSC4
- BioCyc:   PING357804:PING_0535-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52977; Mature: 52846

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKLHVKTWGCQMNEYDSSKMADLLNSTNGYISVEHAEDADVVLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHH
HQLGRWKKLKVNKPNLVIGVGGCVASQEGKAIRSRAPFVDIVFGPQTLHRLPEMIKEVQE
HHHCCCEEEEECCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
NKGTVVDVSFPEIEKFDSLPEPKADGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLLEVAQLAEQGVREVTLLGQNVNSYLGATYDGDTCSFAELLRFVASIDGIDRLRYVTSN
HHHHHHHHHHHCHHEEEHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCC
PIDFSDEIIAVYEDTPELVNFLHLPVQAGSDRILAAMKRGHTVAQYKDQIARLLQVRPEL
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
TVSSDFIIGFPNETDHDFEQTMDLIKYINFDTSYSFIYSQRPGTPAADMPDDVTLETKKQ
EECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEHHHHH
RLAILQDRIQQQSQGIGRKMVGSVQRILVEGPSRKNIMELCGRTENNRIVNFEGDHRSIG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCC
GFVDVEITEVHTNSIRGKFIRGEAEMNLRESIRPSDIVNKHENKSAVNSYIPA
CEEEEEEEEEECCCCCCEEEECCCCCCHHHCCCCHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SMKLHVKTWGCQMNEYDSSKMADLLNSTNGYISVEHAEDADVVLLNTCSIREKAQEKVF
CEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHH
HQLGRWKKLKVNKPNLVIGVGGCVASQEGKAIRSRAPFVDIVFGPQTLHRLPEMIKEVQE
HHHCCCEEEEECCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
NKGTVVDVSFPEIEKFDSLPEPKADGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLLEVAQLAEQGVREVTLLGQNVNSYLGATYDGDTCSFAELLRFVASIDGIDRLRYVTSN
HHHHHHHHHHHCHHEEEHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCC
PIDFSDEIIAVYEDTPELVNFLHLPVQAGSDRILAAMKRGHTVAQYKDQIARLLQVRPEL
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
TVSSDFIIGFPNETDHDFEQTMDLIKYINFDTSYSFIYSQRPGTPAADMPDDVTLETKKQ
EECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEHHHHH
RLAILQDRIQQQSQGIGRKMVGSVQRILVEGPSRKNIMELCGRTENNRIVNFEGDHRSIG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCC
GFVDVEITEVHTNSIRGKFIRGEAEMNLRESIRPSDIVNKHENKSAVNSYIPA
CEEEEEEEEEECCCCCCEEEECCCCCCHHHCCCCHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA