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The map label for this gene is hisH [H]

Identifier: 119899635

GI number: 119899635

Start: 3683868

End: 3684506

Strand: Reverse

Name: hisH [H]

Synonym: azo3346

Alternate gene names: 119899635

Gene position: 3684506-3683868 (Counterclockwise)

Preceding gene: 119899636

Following gene: 119899634

Centisome position: 84.2

GC content: 64.48

Gene sequence:

>639_bases
ATGACCGACGTGGCCATCATCGACTACGGAATGGGCAACCTCCGTTCCGTTGCCAAGGCAATCGAGCACGTTGCGGGTGA
CCGCAGCGTTGTCATTACGTCCGACCCCGCCGTGGTGGCGGCCGCAAAGCGCGTGGTGTTCCCGGGGCAGGGCGCCATGC
CCGACTGCATGCGCGAGCTGGATGCGCGCGGGTTGCGTCCCGTGGTGCTGGAGGCTGCGGCCACCAAGCCCTTTCTCGGG
ATCTGCATCGGCCAGCAGATGCTGTTCGAGCATTCCGCGGAAGGCGATGTGCCGGGGATGTCGATCCTGCCGGGCAAGGT
CGTGCGGTTTCCGGATGAGGGCATGGTTGCGGCCGACGGCGCCCGTCTCAAGGTGCCTCACATGGGCTGGAACGAGGTGT
GGCAGAAGAAGCCGCACCTGCTGTGGGAAGGCATCCCCGACGGCGAACGCTTTTACTTTGTTCACAGCTACTTCGTCGCA
CCCGCCGATGCGGCCTTGACCGCGGCGGAAACCGACTACGGGCTGCGCTTTACCAGTGCGGTAGCCCGGGCTAATATCTT
CGCCGTTCAGTTTCACCCGGAAAAGAGCGCCCAGGCGGGTCTGCGCTTGCTCGACAATTTCATCCGTTGGGCGCCCTGA

Upstream 100 bases:

>100_bases
CGAACGCCTGGCCGGTGCCATTCCCTCCACGAAGGGTGCGCTTTAACGCGCATCCACCCGCGCCCCTTTCTTCACTCATC
TCCTCAGCGAGCGCTCCCAG

Downstream 100 bases:

>100_bases
GCGGCGGCCAGCATCTCCCTCATCTTCACTGCTTCTGTTCCCGGTATGTTGCTGATCCCCGCTATCGACCTGAAGGATGG
TCAATGTGTTCGCCTGAAGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTDVAIIDYGMGNLRSVAKAIEHVAGDRSVVITSDPAVVAAAKRVVFPGQGAMPDCMRELDARGLRPVVLEAAATKPFLG
ICIGQQMLFEHSAEGDVPGMSILPGKVVRFPDEGMVAADGARLKVPHMGWNEVWQKKPHLLWEGIPDGERFYFVHSYFVA
PADAALTAAETDYGLRFTSAVARANIFAVQFHPEKSAQAGLRLLDNFIRWAP

Sequences:

>Translated_212_residues
MTDVAIIDYGMGNLRSVAKAIEHVAGDRSVVITSDPAVVAAAKRVVFPGQGAMPDCMRELDARGLRPVVLEAAATKPFLG
ICIGQQMLFEHSAEGDVPGMSILPGKVVRFPDEGMVAADGARLKVPHMGWNEVWQKKPHLLWEGIPDGERFYFVHSYFVA
PADAALTAAETDYGLRFTSAVARANIFAVQFHPEKSAQAGLRLLDNFIRWAP
>Mature_211_residues
TDVAIIDYGMGNLRSVAKAIEHVAGDRSVVITSDPAVVAAAKRVVFPGQGAMPDCMRELDARGLRPVVLEAAATKPFLGI
CIGQQMLFEHSAEGDVPGMSILPGKVVRFPDEGMVAADGARLKVPHMGWNEVWQKKPHLLWEGIPDGERFYFVHSYFVAP
ADAALTAAETDYGLRFTSAVARANIFAVQFHPEKSAQAGLRLLDNFIRWAP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=39.4230769230769, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=33.1797235023041, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23044; Mature: 22913

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVAIIDYGMGNLRSVAKAIEHVAGDRSVVITSDPAVVAAAKRVVFPGQGAMPDCMREL
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHEEHHHEEEECCCCCCHHHHHHH
DARGLRPVVLEAAATKPFLGICIGQQMLFEHSAEGDVPGMSILPGKVVRFPDEGMVAADG
HHCCCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEECCCCCEEEECC
ARLKVPHMGWNEVWQKKPHLLWEGIPDGERFYFVHSYFVAPADAALTAAETDYGLRFTSA
CEEECCCCCHHHHHHCCCCEEECCCCCCCEEEEEEEEEECCCCHHEEECCCCCCCHHHHH
VARANIFAVQFHPEKSAQAGLRLLDNFIRWAP
HHHCEEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDVAIIDYGMGNLRSVAKAIEHVAGDRSVVITSDPAVVAAAKRVVFPGQGAMPDCMREL
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHEEHHHEEEECCCCCCHHHHHHH
DARGLRPVVLEAAATKPFLGICIGQQMLFEHSAEGDVPGMSILPGKVVRFPDEGMVAADG
HHCCCCCEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEECCCCCEEEECC
ARLKVPHMGWNEVWQKKPHLLWEGIPDGERFYFVHSYFVAPADAALTAAETDYGLRFTSA
CEEECCCCCHHHHHHCCCCEEECCCCCCCEEEEEEEEEECCCCHHEEECCCCCCCHHHHH
VARANIFAVQFHPEKSAQAGLRLLDNFIRWAP
HHHCEEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA