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The map label for this gene is hisA

Identifier: 119899634

GI number: 119899634

Start: 3683067

End: 3683822

Strand: Reverse

Name: hisA

Synonym: azo3345

Alternate gene names: 119899634

Gene position: 3683822-3683067 (Counterclockwise)

Preceding gene: 119899635

Following gene: 119899633

Centisome position: 84.18

GC content: 63.62

Gene sequence:

>756_bases
ATGTTGCTGATCCCCGCTATCGACCTGAAGGATGGTCAATGTGTTCGCCTGAAGCAGGGCGAAATGGACGACGCCACGGT
GTTCTCCGAAGATCCTGCCGCGATGGCGCGGCACTGGATCAAGCAGGGGGCCCGCCGGCTGCATCTCGTCGATCTCAACG
GCGCGTTCGCTGGCAAGCCCAAGAACGGCGGCGCCATTCGCGCGATCACCGACGAAGTGGGTGACGACATCCCCGTTCAG
CTCGGCGGCGGCATTCGTGACCTCGATACCATCGAACACTATCTCGACAACGGCATCTCCTACGTCATCATCGGCACCGC
TGCGGTGAAGAACCCGGGTTTCCTGCACGATGCGTGCGGGGCGTTTCCGGGCCACATCATCGTCGGTCTTGATGCGAAGG
ACGGGAAGGTCGCGGTAGACGGCTGGTCCAAGATGACCGGCCACGATGTCGTCGATCTCGCCAAGAAGTTCGAGGACTAC
GGGGTCGAAGCCGTGATCTACACCGACATCGGCCGTGACGGCATGCTGTCGGGCGTCAATATCGAAGCCACGGTCAGGCT
TGCCCGGGCGCTCCGCATCCCGGTCATTGCCAGCGGTGGCATCGCTTCGCTCACCGACATCGAAGCCCTGTGCGCCGTCG
AGGACGAAGGCATCATGGGGGCGATCACTGGACGTGCAATCTACGAAGGCACGCTCGACCTTTCGGCAGCCCAGTCCCGC
GCAGACGCTCTCCGCGCGCCGGGCGGAGCCGCCTGA

Upstream 100 bases:

>100_bases
GAGCGCCCAGGCGGGTCTGCGCTTGCTCGACAATTTCATCCGTTGGGCGCCCTGAGCGGCGGCCAGCATCTCCCTCATCT
TCACTGCTTCTGTTCCCGGT

Downstream 100 bases:

>100_bases
TGCTGGCAAAGCGCATCATTCCCTGTCTCGACGTCAGCGCAGGCCGGGTCGTCAAGGGCGTCAATTTCGTCGATCTCCGC
GACGCCGGAGATCCGGTCGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLLIPAIDLKDGQCVRLKQGEMDDATVFSEDPAAMARHWIKQGARRLHLVDLNGAFAGKPKNGGAIRAITDEVGDDIPVQ
LGGGIRDLDTIEHYLDNGISYVIIGTAAVKNPGFLHDACGAFPGHIIVGLDAKDGKVAVDGWSKMTGHDVVDLAKKFEDY
GVEAVIYTDIGRDGMLSGVNIEATVRLARALRIPVIASGGIASLTDIEALCAVEDEGIMGAITGRAIYEGTLDLSAAQSR
ADALRAPGGAA

Sequences:

>Translated_251_residues
MLLIPAIDLKDGQCVRLKQGEMDDATVFSEDPAAMARHWIKQGARRLHLVDLNGAFAGKPKNGGAIRAITDEVGDDIPVQ
LGGGIRDLDTIEHYLDNGISYVIIGTAAVKNPGFLHDACGAFPGHIIVGLDAKDGKVAVDGWSKMTGHDVVDLAKKFEDY
GVEAVIYTDIGRDGMLSGVNIEATVRLARALRIPVIASGGIASLTDIEALCAVEDEGIMGAITGRAIYEGTLDLSAAQSR
ADALRAPGGAA
>Mature_251_residues
MLLIPAIDLKDGQCVRLKQGEMDDATVFSEDPAAMARHWIKQGARRLHLVDLNGAFAGKPKNGGAIRAITDEVGDDIPVQ
LGGGIRDLDTIEHYLDNGISYVIIGTAAVKNPGFLHDACGAFPGHIIVGLDAKDGKVAVDGWSKMTGHDVVDLAKKFEDY
GVEAVIYTDIGRDGMLSGVNIEATVRLARALRIPVIASGGIASLTDIEALCAVEDEGIMGAITGRAIYEGTLDLSAAQSR
ADALRAPGGAA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=32.9218106995885, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=22.2222222222222, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_AZOSB (A1KAV5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934847.1
- ProteinModelPortal:   A1KAV5
- SMR:   A1KAV5
- STRING:   A1KAV5
- GeneID:   4609005
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3345
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   A1KAV5
- ProtClustDB:   PRK00748
- BioCyc:   ASP62928:AZO3345-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26301; Mature: 26301

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIPAIDLKDGQCVRLKQGEMDDATVFSEDPAAMARHWIKQGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCC
KNGGAIRAITDEVGDDIPVQLGGGIRDLDTIEHYLDNGISYVIIGTAAVKNPGFLHDACG
CCCCEEEEEHHHHCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHC
AFPGHIIVGLDAKDGKVAVDGWSKMTGHDVVDLAKKFEDYGVEAVIYTDIGRDGMLSGVN
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
IEATVRLARALRIPVIASGGIASLTDIEALCAVEDEGIMGAITGRAIYEGTLDLSAAQSR
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHEEECCCCEEEEEECCEEEECCCCHHHHHHH
ADALRAPGGAA
HHHHCCCCCCC
>Mature Secondary Structure
MLLIPAIDLKDGQCVRLKQGEMDDATVFSEDPAAMARHWIKQGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCC
KNGGAIRAITDEVGDDIPVQLGGGIRDLDTIEHYLDNGISYVIIGTAAVKNPGFLHDACG
CCCCEEEEEHHHHCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHC
AFPGHIIVGLDAKDGKVAVDGWSKMTGHDVVDLAKKFEDYGVEAVIYTDIGRDGMLSGVN
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
IEATVRLARALRIPVIASGGIASLTDIEALCAVEDEGIMGAITGRAIYEGTLDLSAAQSR
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHEEECCCCEEEEEECCEEEECCCCHHHHHHH
ADALRAPGGAA
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA