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The map label for this gene is hisF [H]

Identifier: 119899633

GI number: 119899633

Start: 3682309

End: 3683067

Strand: Reverse

Name: hisF [H]

Synonym: azo3344

Alternate gene names: 119899633

Gene position: 3683067-3682309 (Counterclockwise)

Preceding gene: 119899634

Following gene: 119899632

Centisome position: 84.16

GC content: 65.48

Gene sequence:

>759_bases
ATGCTGGCAAAGCGCATCATTCCCTGTCTCGACGTCAGCGCAGGCCGGGTCGTCAAGGGCGTCAATTTCGTCGATCTCCG
CGACGCCGGAGATCCGGTCGAGGTGGCGCGACGCTACGATGCGCAAGGGGCCGACGAGATCACCTTTCTCGACATCACGG
CCAGCTCCGACGATCGCGACATCATCCTGCACGTGGTCGAGCGGGTTGCGGAACAGGTGTTCATTCCGCTCACTGTAGGC
GGCGGCGTGCGCTCGGTGGAGGACGTCCGGCGCCTGCTCAACGCGGGGGCGGACAAAGTGAGCATCAACACCGCTGCGGT
CAACAACCCTTCCATCGTTGCCGAAGCCAGTGGCAAGGTCGGCAGCCAGTGCATCGTGGTTGCGGTCGACGCCAAGCAGA
CCGGCCCGGGTAAGTGGGAGGTGTTCACCCACGGCGGGCGCAATCCTACCGGGCTCGACGCCATCGAATGGGCGCGCAAG
GTCGAGTCGCTCGGTGCGGGTGAGATCCTGCTGACGAGCATGGACCGCGACGGTACCAAGATCGGCTTCGACCTCGGTCT
GACGCGAGCGGTGTCGGACGCCGTAGGCATTCCGGTCATCGCCAGCGGTGGTGTCGGTTCGCTCGAACACCTTGCGGATG
GCGTCAGCGAGGGGCGGGCGGACGCCGTGCTGGCTGCGAGCATCTTCCATTTCGGTCAGCACACGGTACGCGAGGCGAAG
GAGTTGATGCGGACGCGCGGGATCGAGGTGCGGCTGTGA

Upstream 100 bases:

>100_bases
TGGGGGCGATCACTGGACGTGCAATCTACGAAGGCACGCTCGACCTTTCGGCAGCCCAGTCCCGCGCAGACGCTCTCCGC
GCGCCGGGCGGAGCCGCCTG

Downstream 100 bases:

>100_bases
GTACGAGCACCAAGTGGCTCAACGAGGTGAAGTGGGACGAAAACGGCCTGGTGCCGGTGATCGCCCAGGAGGCCTCCACG
GGCGACGTGCTGATGTTCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVSAGRVVKGVNFVDLRDAGDPVEVARRYDAQGADEITFLDITASSDDRDIILHVVERVAEQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSINTAAVNNPSIVAEASGKVGSQCIVVAVDAKQTGPGKWEVFTHGGRNPTGLDAIEWARK
VESLGAGEILLTSMDRDGTKIGFDLGLTRAVSDAVGIPVIASGGVGSLEHLADGVSEGRADAVLAASIFHFGQHTVREAK
ELMRTRGIEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVSAGRVVKGVNFVDLRDAGDPVEVARRYDAQGADEITFLDITASSDDRDIILHVVERVAEQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSINTAAVNNPSIVAEASGKVGSQCIVVAVDAKQTGPGKWEVFTHGGRNPTGLDAIEWARK
VESLGAGEILLTSMDRDGTKIGFDLGLTRAVSDAVGIPVIASGGVGSLEHLADGVSEGRADAVLAASIFHFGQHTVREAK
ELMRTRGIEVRL
>Mature_252_residues
MLAKRIIPCLDVSAGRVVKGVNFVDLRDAGDPVEVARRYDAQGADEITFLDITASSDDRDIILHVVERVAEQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSINTAAVNNPSIVAEASGKVGSQCIVVAVDAKQTGPGKWEVFTHGGRNPTGLDAIEWARK
VESLGAGEILLTSMDRDGTKIGFDLGLTRAVSDAVGIPVIASGGVGSLEHLADGVSEGRADAVLAASIFHFGQHTVREAK
ELMRTRGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.8015564202335, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=30, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.624203821656, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26588; Mature: 26588

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVSAGRVVKGVNFVDLRDAGDPVEVARRYDAQGADEITFLDITASSDDRD
CCHHHCCCCCCCCCCCEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHH
IILHVVERVAEQVFIPLTVGGGVRSVEDVRRLLNAGADKVSINTAAVNNPSIVAEASGKV
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
GSQCIVVAVDAKQTGPGKWEVFTHGGRNPTGLDAIEWARKVESLGAGEILLTSMDRDGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCE
IGFDLGLTRAVSDAVGIPVIASGGVGSLEHLADGVSEGRADAVLAASIFHFGQHTVREAK
EEHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELMRTRGIEVRL
HHHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVSAGRVVKGVNFVDLRDAGDPVEVARRYDAQGADEITFLDITASSDDRD
CCHHHCCCCCCCCCCCEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHH
IILHVVERVAEQVFIPLTVGGGVRSVEDVRRLLNAGADKVSINTAAVNNPSIVAEASGKV
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
GSQCIVVAVDAKQTGPGKWEVFTHGGRNPTGLDAIEWARKVESLGAGEILLTSMDRDGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCE
IGFDLGLTRAVSDAVGIPVIASGGVGSLEHLADGVSEGRADAVLAASIFHFGQHTVREAK
EEHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELMRTRGIEVRL
HHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA