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The map label for this gene is pykA [H]

Identifier: 119899132

GI number: 119899132

Start: 3120337

End: 3121779

Strand: Direct

Name: pykA [H]

Synonym: azo2842

Alternate gene names: 119899132

Gene position: 3120337-3121779 (Clockwise)

Preceding gene: 119899129

Following gene: 119899133

Centisome position: 71.31

GC content: 69.16

Gene sequence:

>1443_bases
ATGCCCCGCCACACCAAGATCGTCGCCACCCTCGGCCCGGCCTCCTCCGACCCGCAGGTACTCGAACGCATGGTCCACGC
CGGCGTGGACGTGGTGCGGATGAACTTCTCCCACGGCAAGGCGGAAGACCATGTCGCCCGCGCCGCGGCGATCCGCGAGG
CGTCGGCGCGTGCCGGACGGCCGGTCGGCATCCTCGCCGACCTGCAGGGGCCGAAGATCCGCGTCGGCAAGTTCGAGAAC
GGCCGGGTGACGCTGACCCGCGACGCCGAGTTCATCCTCGACGCCCACTGCAACCTCGGCAACGGCCAGCGCGTGGGGCT
GGACTACAAGGACCTGCCCAAGGACGTGCGCGCCGGCGACATCCTGCTGCTCGACGACGGCCGCCTCAAGCTGCAGGTGG
AGCGCGTGCTCGGCGCCGAGATCCACACCCGGGTACGGGTCGGAGGCGAGTTGTCCAACAACAAGGGCATCAACCGCCAG
GGCGGCGGCCTCACCGCGCCGGCGCTCACCGCCAAGGACATGGACGACATCAAGACCGCAGCGCAGATCGGCGTCGATTT
CCTCGCGGTGTCCTTCCCCAAGAGCGCGGCCGACATGTACATGGCCCGCCAGCTGATGCGCGCGGCCGGCGGCGACGCGC
TGCTGATCGCGAAGATCGAGCGCACCGAGGCGGTGGCGAACCTGGAGGAAATCCTCGCCGCCTCCGACGGCATCATGGTG
GCGCGCGGCGACCTCGCGGTGGAGGTGGGCGACGCCGCGGTGCCGGCGCTGCAGAAGAAGATGATCCGCGCCGCGCGCGA
CCACAACAAGCTGACGATCACCGCGACCCAGATGATGGAGTCGATGATCACCAGCCCGGTGCCGACCCGCGCCGAGGTGT
CGGACGTCGCCAACGCGGTGCTCGACGGCACCGATGCGGTGATGCTGTCGGCCGAGACCGCCGCCGGCAGCTTTCCGGTG
GAAGTCGTCGAGGCGATGAGCCGCGTCTGCCTGGAAGCCGAGAAGTCGGCCGAGGTCAGCCTCGACCGCGAGGTGCTCGA
CCGGGTGTTCACCCGCATCGACCAGTCGATCGCGATGGCAGCGATCTGGACCGCCTGGCACCTGAAGGTGAAGGCGGTCG
CCTCGCTCACCCAGACCGGCTCCACCGCGCTGTGGATGAGCCGGCTCAACTGCGGCGTGCCGATCTACGCGCTGACGCCC
GAAATCGGCGCGCGCAACCAGATGACGCTCTACCGCGAGGTCTATCCGCTGCTGATGAGCCAGACCCACTTCGACCGCGA
CGTGCTGCTGTGGGAAGCCGAGCAGGTGCTGCTCGACCAGGGCGTGGTGGAATACGGCGACCTCATCGTGCTCACCATCG
GCGAGCCGATCGGCGCCTCGGGCGGCACCAACACCCTCAAGATCGTGCGCGTGGGCGAACACAAGGCACCCGGCGTGCAG
TAA

Upstream 100 bases:

>100_bases
CAAGGATAGATCAGGCTCGCGCTAGAATCCGGGCCCGGGACGTAAGCGCACCTTTCCGCCGCCCTGCCGGTCGCGGAGCA
AAAACCAGACAGGAGACAGC

Downstream 100 bases:

>100_bases
GCGCCGCGTATGTCTCAAAGCGCAAACGCGCTTACCGCCCCGCACCGCGCCCGGATAAAATGCCGGTTTCCGTATCGACC
AACACAGGCGGCGGCCTGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPRHTKIVATLGPASSDPQVLERMVHAGVDVVRMNFSHGKAEDHVARAAAIREASARAGRPVGILADLQGPKIRVGKFEN
GRVTLTRDAEFILDAHCNLGNGQRVGLDYKDLPKDVRAGDILLLDDGRLKLQVERVLGAEIHTRVRVGGELSNNKGINRQ
GGGLTAPALTAKDMDDIKTAAQIGVDFLAVSFPKSAADMYMARQLMRAAGGDALLIAKIERTEAVANLEEILAASDGIMV
ARGDLAVEVGDAAVPALQKKMIRAARDHNKLTITATQMMESMITSPVPTRAEVSDVANAVLDGTDAVMLSAETAAGSFPV
EVVEAMSRVCLEAEKSAEVSLDREVLDRVFTRIDQSIAMAAIWTAWHLKVKAVASLTQTGSTALWMSRLNCGVPIYALTP
EIGARNQMTLYREVYPLLMSQTHFDRDVLLWEAEQVLLDQGVVEYGDLIVLTIGEPIGASGGTNTLKIVRVGEHKAPGVQ

Sequences:

>Translated_480_residues
MPRHTKIVATLGPASSDPQVLERMVHAGVDVVRMNFSHGKAEDHVARAAAIREASARAGRPVGILADLQGPKIRVGKFEN
GRVTLTRDAEFILDAHCNLGNGQRVGLDYKDLPKDVRAGDILLLDDGRLKLQVERVLGAEIHTRVRVGGELSNNKGINRQ
GGGLTAPALTAKDMDDIKTAAQIGVDFLAVSFPKSAADMYMARQLMRAAGGDALLIAKIERTEAVANLEEILAASDGIMV
ARGDLAVEVGDAAVPALQKKMIRAARDHNKLTITATQMMESMITSPVPTRAEVSDVANAVLDGTDAVMLSAETAAGSFPV
EVVEAMSRVCLEAEKSAEVSLDREVLDRVFTRIDQSIAMAAIWTAWHLKVKAVASLTQTGSTALWMSRLNCGVPIYALTP
EIGARNQMTLYREVYPLLMSQTHFDRDVLLWEAEQVLLDQGVVEYGDLIVLTIGEPIGASGGTNTLKIVRVGEHKAPGVQ
>Mature_479_residues
PRHTKIVATLGPASSDPQVLERMVHAGVDVVRMNFSHGKAEDHVARAAAIREASARAGRPVGILADLQGPKIRVGKFENG
RVTLTRDAEFILDAHCNLGNGQRVGLDYKDLPKDVRAGDILLLDDGRLKLQVERVLGAEIHTRVRVGGELSNNKGINRQG
GGLTAPALTAKDMDDIKTAAQIGVDFLAVSFPKSAADMYMARQLMRAAGGDALLIAKIERTEAVANLEEILAASDGIMVA
RGDLAVEVGDAAVPALQKKMIRAARDHNKLTITATQMMESMITSPVPTRAEVSDVANAVLDGTDAVMLSAETAAGSFPVE
VVEAMSRVCLEAEKSAEVSLDREVLDRVFTRIDQSIAMAAIWTAWHLKVKAVASLTQTGSTALWMSRLNCGVPIYALTPE
IGARNQMTLYREVYPLLMSQTHFDRDVLLWEAEQVLLDQGVVEYGDLIVLTIGEPIGASGGTNTLKIVRVGEHKAPGVQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=35.5691056910569, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=35.5691056910569, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.8635437881874, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.8635437881874, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=35.9918200408998, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=35.5400696864112, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=35.5400696864112, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.7659574468085, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.7659574468085, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.7757009345794, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=53.6534446764092, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=36.8869936034115, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=37.782340862423, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=35.4378818737271, Blast_Score=258, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=35.4378818737271, Blast_Score=258, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=35.4378818737271, Blast_Score=257, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=35.4378818737271, Blast_Score=257, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=34.1614906832298, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=34.826883910387, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=37.7281947261663, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=37.7281947261663, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=37.5903614457831, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=32.0408163265306, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24646914, Length=273, Percent_Identity=38.8278388278388, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51560; Mature: 51429

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRHTKIVATLGPASSDPQVLERMVHAGVDVVRMNFSHGKAEDHVARAAAIREASARAGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
PVGILADLQGPKIRVGKFENGRVTLTRDAEFILDAHCNLGNGQRVGLDYKDLPKDVRAGD
CEEEEEECCCCEEEEEEECCCCEEEEECCEEEEEECCCCCCCCEECCCHHHCCCCCCCCC
ILLLDDGRLKLQVERVLGAEIHTRVRVGGELSNNKGINRQGGGLTAPALTAKDMDDIKTA
EEEEECCCEEEEEEHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AQIGVDFLAVSFPKSAADMYMARQLMRAAGGDALLIAKIERTEAVANLEEILAASDGIMV
HHHCHHEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEE
ARGDLAVEVGDAAVPALQKKMIRAARDHNKLTITATQMMESMITSPVPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAETAAGSFPVEVVEAMSRVCLEAEKSAEVSLDREVLDRVFTRIDQSIAMA
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHH
AIWTAWHLKVKAVASLTQTGSTALWMSRLNCGVPIYALTPEIGARNQMTLYREVYPLLMS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
QTHFDRDVLLWEAEQVLLDQGVVEYGDLIVLTIGEPIGASGGTNTLKIVRVGEHKAPGVQ
CCCCCCEEEEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
PRHTKIVATLGPASSDPQVLERMVHAGVDVVRMNFSHGKAEDHVARAAAIREASARAGR
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
PVGILADLQGPKIRVGKFENGRVTLTRDAEFILDAHCNLGNGQRVGLDYKDLPKDVRAGD
CEEEEEECCCCEEEEEEECCCCEEEEECCEEEEEECCCCCCCCEECCCHHHCCCCCCCCC
ILLLDDGRLKLQVERVLGAEIHTRVRVGGELSNNKGINRQGGGLTAPALTAKDMDDIKTA
EEEEECCCEEEEEEHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AQIGVDFLAVSFPKSAADMYMARQLMRAAGGDALLIAKIERTEAVANLEEILAASDGIMV
HHHCHHEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEE
ARGDLAVEVGDAAVPALQKKMIRAARDHNKLTITATQMMESMITSPVPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAETAAGSFPVEVVEAMSRVCLEAEKSAEVSLDREVLDRVFTRIDQSIAMA
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHH
AIWTAWHLKVKAVASLTQTGSTALWMSRLNCGVPIYALTPEIGARNQMTLYREVYPLLMS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
QTHFDRDVLLWEAEQVLLDQGVVEYGDLIVLTIGEPIGASGGTNTLKIVRVGEHKAPGVQ
CCCCCCEEEEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]