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The map label for this gene is paaF2 [C]

Identifier: 119897987

GI number: 119897987

Start: 1865171

End: 1865971

Strand: Direct

Name: paaF2 [C]

Synonym: azo1696

Alternate gene names: 119897987

Gene position: 1865171-1865971 (Clockwise)

Preceding gene: 119897986

Following gene: 119897988

Centisome position: 42.62

GC content: 64.79

Gene sequence:

>801_bases
ATGAGCGCATTGCCCCATACGGCCGATGCTCCGCTGGTCGTGCGCAGCGACCGCGAAGGCGTCACCACCCTTACCCTCAA
CCGCCCGGCGCAGTTCAACGCCTTGTCCGAGGCGATGCTGAACGCGCTGATCACCGAGATCGATGCGATTGCGCTCGATC
CTACCGTGCGCGTCGTCGTGCTCGCGGGCGAAGGCAAAGCCTTCTGCGCCGGTCACGACCTGAAAGAGATGCGGGCCAAC
CACAACCTCGCTTTCCAGCAGGCCCTGTTCCGGTTGTGCAGCAAGTTCATGCTGAAGCTCACCGAGCTGCCGCAGCCGGT
GATCGCCCGCATCCACGGCATCGCCACCGCGGCCGGGTGCCAGCTTGTTTCCATGTGCGACCTCGCGGTGGCGGCCGATG
TCGCGCGCTTCGCCGTTTCCGGCATCAATGTGGGCTTGTTCTGTGCCACACCCAGCGTCGGTCTGTCGCGCAATCTCGGT
CGCAAGGAAGCGTTCGAGATGCTGGTGACGGGCGATTTCATCGACGCCGCCGAGGCGCAGCGCCGCGGGCTGGTGAATCG
CGTCGTGCCGCTCGAACAGCTCGATGAGGAGATCGCGCGTCTGGCGGCGGCCATCTGCGCAAAATCGCCGCTCGCGATCC
GGATGGGCAAGCAGATGTTCTACAAGCAGCTGGAGATGGGTATGGAAGCGGCCTACCAGCTGGCCGGCGAAACGATGGCG
TGCAACATGATGGCGGACGACGCCGCCGAAGGCATCGATGCTTTCATCGCCAAGCGCAAGCCGGAGTGGTCGGGACGCTG
A

Upstream 100 bases:

>100_bases
CGCTCGGGCGGGTTCCTGTTCCGGCCGGCGCCTCCGACGGGTGCGCCGGCCCAGTTGCTGCATCAGGGCCCGCGCGAGGC
CCTCAATCCCAGGAGGAGAC

Downstream 100 bases:

>100_bases
GCGTGGCCTCACCCGCGGGTGTCGATCCAGCCGAGCCGGAATTCGGGCTGGTCGACACCCACATCCATCTCGACGCTGCT
GAGTTCGACGCAGACCGTGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSALPHTADAPLVVRSDREGVTTLTLNRPAQFNALSEAMLNALITEIDAIALDPTVRVVVLAGEGKAFCAGHDLKEMRAN
HNLAFQQALFRLCSKFMLKLTELPQPVIARIHGIATAAGCQLVSMCDLAVAADVARFAVSGINVGLFCATPSVGLSRNLG
RKEAFEMLVTGDFIDAAEAQRRGLVNRVVPLEQLDEEIARLAAAICAKSPLAIRMGKQMFYKQLEMGMEAAYQLAGETMA
CNMMADDAAEGIDAFIAKRKPEWSGR

Sequences:

>Translated_266_residues
MSALPHTADAPLVVRSDREGVTTLTLNRPAQFNALSEAMLNALITEIDAIALDPTVRVVVLAGEGKAFCAGHDLKEMRAN
HNLAFQQALFRLCSKFMLKLTELPQPVIARIHGIATAAGCQLVSMCDLAVAADVARFAVSGINVGLFCATPSVGLSRNLG
RKEAFEMLVTGDFIDAAEAQRRGLVNRVVPLEQLDEEIARLAAAICAKSPLAIRMGKQMFYKQLEMGMEAAYQLAGETMA
CNMMADDAAEGIDAFIAKRKPEWSGR
>Mature_265_residues
SALPHTADAPLVVRSDREGVTTLTLNRPAQFNALSEAMLNALITEIDAIALDPTVRVVVLAGEGKAFCAGHDLKEMRANH
NLAFQQALFRLCSKFMLKLTELPQPVIARIHGIATAAGCQLVSMCDLAVAADVARFAVSGINVGLFCATPSVGLSRNLGR
KEAFEMLVTGDFIDAAEAQRRGLVNRVVPLEQLDEEIARLAAAICAKSPLAIRMGKQMFYKQLEMGMEAAYQLAGETMAC
NMMADDAAEGIDAFIAKRKPEWSGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=44.7154471544715, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=35.6, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI260274832, Length=261, Percent_Identity=24.5210727969349, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI45643119, Length=261, Percent_Identity=24.5210727969349, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260275230, Length=261, Percent_Identity=24.5210727969349, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=29.4820717131474, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757966, Length=252, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538581, Length=252, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI25453481, Length=252, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538573, Length=252, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI37594469, Length=200, Percent_Identity=30.5, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757968, Length=194, Percent_Identity=24.2268041237113, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI49355787, Length=194, Percent_Identity=24.2268041237113, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=36.5384615384615, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=31.7269076305221, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=29.7709923664122, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI221142681, Length=222, Percent_Identity=33.7837837837838, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=27.6264591439689, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=26.5193370165746, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=39.9141630901288, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17554946, Length=274, Percent_Identity=28.8321167883212, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=33.7254901960784, Blast_Score=126, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17536985, Length=279, Percent_Identity=27.2401433691756, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=34.2995169082126, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.9620853080569, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=26.984126984127, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=211, Percent_Identity=27.9620853080569, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=208, Percent_Identity=24.5192307692308, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=38.4030418250951, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=32.5581395348837, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=32.5581395348837, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=29.811320754717, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19920382, Length=276, Percent_Identity=27.536231884058, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24650670, Length=240, Percent_Identity=30.4166666666667, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357171, Length=187, Percent_Identity=30.4812834224599, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28651; Mature: 28520

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPHTADAPLVVRSDREGVTTLTLNRPAQFNALSEAMLNALITEIDAIALDPTVRVVV
CCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
LAGEGKAFCAGHDLKEMRANHNLAFQQALFRLCSKFMLKLTELPQPVIARIHGIATAAGC
EECCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVSMCDLAVAADVARFAVSGINVGLFCATPSVGLSRNLGRKEAFEMLVTGDFIDAAEAQ
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHH
RRGLVNRVVPLEQLDEEIARLAAAICAKSPLAIRMGKQMFYKQLEMGMEAAYQLAGETMA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
CNMMADDAAEGIDAFIAKRKPEWSGR
EHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SALPHTADAPLVVRSDREGVTTLTLNRPAQFNALSEAMLNALITEIDAIALDPTVRVVV
CCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
LAGEGKAFCAGHDLKEMRANHNLAFQQALFRLCSKFMLKLTELPQPVIARIHGIATAAGC
EECCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVSMCDLAVAADVARFAVSGINVGLFCATPSVGLSRNLGRKEAFEMLVTGDFIDAAEAQ
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHH
RRGLVNRVVPLEQLDEEIARLAAAICAKSPLAIRMGKQMFYKQLEMGMEAAYQLAGETMA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
CNMMADDAAEGIDAFIAKRKPEWSGR
EHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA