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The map label for this gene is yngI [H]

Identifier: 119897986

GI number: 119897986

Start: 1863402

End: 1865054

Strand: Direct

Name: yngI [H]

Synonym: azo1695

Alternate gene names: 119897986

Gene position: 1863402-1865054 (Clockwise)

Preceding gene: 119897985

Following gene: 119897987

Centisome position: 42.58

GC content: 64.13

Gene sequence:

>1653_bases
ATGACCGAGAGGAAGCAGACGCCCTACGACGTGGGGCTGGAGAAAAACGCCGCCAACTATGTACCGCTGACCCCGCTCAC
GTTCATCGAGCGCAGCGCCTACATCTACCCCGACCGCGTCGCCGTGATCCACGGCAAGCGTCGCTACACCTGGCTGGAAA
GCTACACGCGGTCGCGCCGTCTCGCGTCGGCGCTGAAGCAACTGGGCGTGGGCAAGAACGACACGGTCGCGGTGATCCTG
AACAACACGCCGGAAATGTTCGAGTGCCATTTCGGCGTGCCTGCGTGCGGCGCCGTGCTGAACACCGTCAACACCCGGCT
CGACGCGGAAGGCGTGGCCTTCATCCTCAACCACGGCGAAGCCAAGGTGCTGATTACCGACCGCGAGTATTCGCGCATGG
TCGGCAAGGCGATCGAACTGGCGAATCGCCCGGACATGATCGTGATCGACGTGGATGATCCCGAATACACCGGTCCGGGC
GAACGCGTCGGCAAGCTCGACTACGAGGCCCTGCTGGAAACCGGCAATCCCGAATTCGAGTATGAGCAGCCGGGCGACGA
GTGGGATGCGATCTCACTCAACTACACCTCCGGCACCACCGGCAACCCCAAGGGCGTGGTCTATCACCATCGCGGTGCCT
ACCTCAACGCGATGTCCAACATCGTGTCGTGGGGCATGCCGCCGCACTCGGTGTATCTGTGGACGCTGCCGATGTTCCAT
TGCAACGGCTGGTGCTTCGCCTGGACCATGGCGGCCAATGCCGGCGTCAACGTCTGCCTGCGCCGTGTCGACCCGCGGCT
GATCTTCGACGCGATGCGCGAGCACGGCGTCACCCACTACTGCGGCGCGCCCATCGTGCATTCCATGCTCGCCAATGCGC
CCGAAGAGTGGCGCAAGGGTATCAATCACAAGGTCTCGGGCCTGGTCGCGGCGGCGCCGCCGCCGGCCGCGGTGATCGAG
GGCATGGCCAAGATCGGCATCGACATCACCCACGTCTATGGGCTGACCGAAACCTACGGTCCGGCCTCGGTGTGCGCCAA
GCACGACGAATGGCGCGATCTGCCGCTGGCCGAGCAGGTTGCCAAGAACGGTCGCCAGGGCGTGCGCTATCACGCCCAGG
AAGGCATCACGGTGCTGGACCCGACCACGATGGAACCCGTGCCCTGGGACAACGAGACCATGGGCGAGATCATGTTCCGC
GGCAACCTGGTCATGAAGGGCTACCTGAAGAACGAGAAGGCCACCGAGGAGTCCTTCCGCGGCGGCTGGTATCACACCGG
CGACCTGGCGGTGATGCAGCCGGACGGCTACGTCAAGATCAAGGACCGTTCGAAGGACGTCATCATCTCGGGCGGTGAGA
ACATCTCGTCGATCGAGGTCGAAGATGCGCTGTACAAGCACCCTGCGGTGATGGCCGCCGCGGTGGTGGCCGCGCCCGAC
GAGAAGTGGGGCGAGGTGCCGTGCGCCTTCGTCGAACTGAAGGATGGCGCGACCGTGACCGCCGAGGAGATCATTGCCCA
CTGCCGCGAGCACTTGGCGGGCTTCAAGACGCCGAAAAAGGTGATCTTCGGCGCGCTGCCCAAGACCTCGACCGGCAAGA
TCCAGAAATTCGTGCTGCGCGAGCAGGCGAAGTCCTCGTCCGCCATCGAGTAA

Upstream 100 bases:

>100_bases
TGACCATGACGAGCGGCAAGGTTTGAATACCGGAGGGCGCTGCGGGCAGTGAGACGCGGCGACCCCAAGATCTTATAAAA
CGCATAACAGCGGAGTCGAT

Downstream 100 bases:

>100_bases
TGGCGTGCATGGGACCCGCTCGGGCGGGTTCCTGTTCCGGCCGGCGCCTCCGACGGGTGCGCCGGCCCAGTTGCTGCATC
AGGGCCCGCGCGAGGCCCTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTERKQTPYDVGLEKNAANYVPLTPLTFIERSAYIYPDRVAVIHGKRRYTWLESYTRSRRLASALKQLGVGKNDTVAVIL
NNTPEMFECHFGVPACGAVLNTVNTRLDAEGVAFILNHGEAKVLITDREYSRMVGKAIELANRPDMIVIDVDDPEYTGPG
ERVGKLDYEALLETGNPEFEYEQPGDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAMSNIVSWGMPPHSVYLWTLPMFH
CNGWCFAWTMAANAGVNVCLRRVDPRLIFDAMREHGVTHYCGAPIVHSMLANAPEEWRKGINHKVSGLVAAAPPPAAVIE
GMAKIGIDITHVYGLTETYGPASVCAKHDEWRDLPLAEQVAKNGRQGVRYHAQEGITVLDPTTMEPVPWDNETMGEIMFR
GNLVMKGYLKNEKATEESFRGGWYHTGDLAVMQPDGYVKIKDRSKDVIISGGENISSIEVEDALYKHPAVMAAAVVAAPD
EKWGEVPCAFVELKDGATVTAEEIIAHCREHLAGFKTPKKVIFGALPKTSTGKIQKFVLREQAKSSSAIE

Sequences:

>Translated_550_residues
MTERKQTPYDVGLEKNAANYVPLTPLTFIERSAYIYPDRVAVIHGKRRYTWLESYTRSRRLASALKQLGVGKNDTVAVIL
NNTPEMFECHFGVPACGAVLNTVNTRLDAEGVAFILNHGEAKVLITDREYSRMVGKAIELANRPDMIVIDVDDPEYTGPG
ERVGKLDYEALLETGNPEFEYEQPGDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAMSNIVSWGMPPHSVYLWTLPMFH
CNGWCFAWTMAANAGVNVCLRRVDPRLIFDAMREHGVTHYCGAPIVHSMLANAPEEWRKGINHKVSGLVAAAPPPAAVIE
GMAKIGIDITHVYGLTETYGPASVCAKHDEWRDLPLAEQVAKNGRQGVRYHAQEGITVLDPTTMEPVPWDNETMGEIMFR
GNLVMKGYLKNEKATEESFRGGWYHTGDLAVMQPDGYVKIKDRSKDVIISGGENISSIEVEDALYKHPAVMAAAVVAAPD
EKWGEVPCAFVELKDGATVTAEEIIAHCREHLAGFKTPKKVIFGALPKTSTGKIQKFVLREQAKSSSAIE
>Mature_549_residues
TERKQTPYDVGLEKNAANYVPLTPLTFIERSAYIYPDRVAVIHGKRRYTWLESYTRSRRLASALKQLGVGKNDTVAVILN
NTPEMFECHFGVPACGAVLNTVNTRLDAEGVAFILNHGEAKVLITDREYSRMVGKAIELANRPDMIVIDVDDPEYTGPGE
RVGKLDYEALLETGNPEFEYEQPGDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAMSNIVSWGMPPHSVYLWTLPMFHC
NGWCFAWTMAANAGVNVCLRRVDPRLIFDAMREHGVTHYCGAPIVHSMLANAPEEWRKGINHKVSGLVAAAPPPAAVIEG
MAKIGIDITHVYGLTETYGPASVCAKHDEWRDLPLAEQVAKNGRQGVRYHAQEGITVLDPTTMEPVPWDNETMGEIMFRG
NLVMKGYLKNEKATEESFRGGWYHTGDLAVMQPDGYVKIKDRSKDVIISGGENISSIEVEDALYKHPAVMAAAVVAAPDE
KWGEVPCAFVELKDGATVTAEEIIAHCREHLAGFKTPKKVIFGALPKTSTGKIQKFVLREQAKSSSAIE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=29.7101449275362, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=26.5873015873016, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=26.5873015873016, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=27.7673545966229, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=26.3888888888889, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=27.5728155339806, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI38505220, Length=515, Percent_Identity=27.378640776699, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=25.4578754578755, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=25.4578754578755, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI115511026, Length=523, Percent_Identity=26.1950286806883, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28416953, Length=537, Percent_Identity=24.3947858472998, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI13375727, Length=556, Percent_Identity=24.2805755395683, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI253970489, Length=128, Percent_Identity=39.84375, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8923896, Length=128, Percent_Identity=39.84375, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=568, Percent_Identity=27.112676056338, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI221142682, Length=496, Percent_Identity=27.2177419354839, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI145693145, Length=535, Percent_Identity=28.785046728972, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786810, Length=542, Percent_Identity=26.0147601476015, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788595, Length=362, Percent_Identity=26.7955801104972, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790505, Length=129, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=28.8321167883212, Blast_Score=189, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71994694, Length=548, Percent_Identity=28.8321167883212, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71994690, Length=548, Percent_Identity=28.8321167883212, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=28.1021897810219, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=27.8210116731518, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=25.4826254826255, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=24.8106060606061, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=538, Percent_Identity=25.8364312267658, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=25.8003766478343, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=495, Percent_Identity=23.2323232323232, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=511, Percent_Identity=23.2876712328767, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982997, Length=550, Percent_Identity=26.1818181818182, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=550, Percent_Identity=26.1818181818182, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=519, Percent_Identity=23.5067437379576, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=607, Percent_Identity=23.0642504118616, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=509, Percent_Identity=25.9332023575639, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=543, Percent_Identity=27.6243093922652, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323182, Length=142, Percent_Identity=37.3239436619718, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581924, Length=553, Percent_Identity=26.5822784810127, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=26.7552182163188, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI18859661, Length=522, Percent_Identity=28.3524904214559, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24656500, Length=533, Percent_Identity=25.140712945591, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24583465, Length=471, Percent_Identity=24.6284501061571, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583463, Length=471, Percent_Identity=24.6284501061571, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356947, Length=515, Percent_Identity=22.9126213592233, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364798, Length=471, Percent_Identity=24.6284501061571, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933690, Length=536, Percent_Identity=22.5746268656716, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=21.8503937007874, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=484, Percent_Identity=23.1404958677686, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648255, Length=504, Percent_Identity=21.8253968253968, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.6452905811623, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=21.484375, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130357, Length=162, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60793; Mature: 60662

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKQTPYDVGLEKNAANYVPLTPLTFIERSAYIYPDRVAVIHGKRRYTWLESYTRSRR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCEEEEEECCCHHHHHHHHHHHHH
LASALKQLGVGKNDTVAVILNNTPEMFECHFGVPACGAVLNTVNTRLDAEGVAFILNHGE
HHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCC
AKVLITDREYSRMVGKAIELANRPDMIVIDVDDPEYTGPGERVGKLDYEALLETGNPEFE
EEEEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHCCCCCCC
YEQPGDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAMSNIVSWGMPPHSVYLWTLPMFH
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEEHH
CNGWCFAWTMAANAGVNVCLRRVDPRLIFDAMREHGVTHYCGAPIVHSMLANAPEEWRKG
CCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCEECCHHHHHHHHHCCCHHHHHC
INHKVSGLVAAAPPPAAVIEGMAKIGIDITHVYGLTETYGPASVCAKHDEWRDLPLAEQV
CCHHHCEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHH
AKNGRQGVRYHAQEGITVLDPTTMEPVPWDNETMGEIMFRGNLVMKGYLKNEKATEESFR
HHCCCCCCEEHHHCCCEEECCCCCCCCCCCCCCHHHEEEECCEEEEEEECCCCCCHHHHC
GGWYHTGDLAVMQPDGYVKIKDRSKDVIISGGENISSIEVEDALYKHPAVMAAAVVAAPD
CCCEEECCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCC
EKWGEVPCAFVELKDGATVTAEEIIAHCREHLAGFKTPKKVIFGALPKTSTGKIQKFVLR
HHCCCCCEEEEEECCCCEECHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHH
EQAKSSSAIE
HHHCCCCCCC
>Mature Secondary Structure 
TERKQTPYDVGLEKNAANYVPLTPLTFIERSAYIYPDRVAVIHGKRRYTWLESYTRSRR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCEEEEEECCCHHHHHHHHHHHHH
LASALKQLGVGKNDTVAVILNNTPEMFECHFGVPACGAVLNTVNTRLDAEGVAFILNHGE
HHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCC
AKVLITDREYSRMVGKAIELANRPDMIVIDVDDPEYTGPGERVGKLDYEALLETGNPEFE
EEEEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHCCCCCCC
YEQPGDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAMSNIVSWGMPPHSVYLWTLPMFH
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEEHH
CNGWCFAWTMAANAGVNVCLRRVDPRLIFDAMREHGVTHYCGAPIVHSMLANAPEEWRKG
CCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCEECCHHHHHHHHHCCCHHHHHC
INHKVSGLVAAAPPPAAVIEGMAKIGIDITHVYGLTETYGPASVCAKHDEWRDLPLAEQV
CCHHHCEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHH
AKNGRQGVRYHAQEGITVLDPTTMEPVPWDNETMGEIMFRGNLVMKGYLKNEKATEESFR
HHCCCCCCEEHHHCCCEEECCCCCCCCCCCCCCHHHEEEECCEEEEEEECCCCCCHHHHC
GGWYHTGDLAVMQPDGYVKIKDRSKDVIISGGENISSIEVEDALYKHPAVMAAAVVAAPD
CCCEEECCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCC
EKWGEVPCAFVELKDGATVTAEEIIAHCREHLAGFKTPKKVIFGALPKTSTGKIQKFVLR
HHCCCCCEEEEEECCCCEECHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHH
EQAKSSSAIE
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]