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The map label for this gene is rpoS [H]

Identifier: 119897381

GI number: 119897381

Start: 1172633

End: 1173568

Strand: Direct

Name: rpoS [H]

Synonym: azo1090

Alternate gene names: 119897381

Gene position: 1172633-1173568 (Clockwise)

Preceding gene: 119897380

Following gene: 119897386

Centisome position: 26.8

GC content: 61.86

Gene sequence:

>936_bases
ATGGAAGATCCGGTTAGCTCTGACGAACTCGAAAGTCATGAACCGGATCTCCCGCTGGAGGTCGAGGTGTTTGCGGAGCC
GCATGCACCGGTATTCGAGAACGACTTTCTCAGCGACGTCACCCAGATCTACCTGAACGAAATCGGCGCCAACCCGCTGC
TGTCGGCAGAAGAGGAGGCCGCGCTGGCGCGCCGGGTGCGCGCGGGCGATTTCGATGCGCGTCAGACGATGATCGAGCGC
AACCTCCGCCTCGTCGTCAACATCGCCAAGCACTACCTTAATCGCGGCATCCCGCTGCTTGATCTGGTCGAGGAAGGCAA
CCTCGGACTGATGCATGCGCTGGAGAAGTTCGACCCCGAGCGCGGATTCCGTTTTTCCACCTACGCAACCTGGTGGATTC
GCCAGAACATCGAGCGCGCGATCATGAACCAGTCGCGCACGATCCGCTTGCCTGTCCATGTGGTCAAGGAGTTGAACCAG
GTGTTGCGTGCGCAACGCGTGGTCGAGGCGGCTTCGAACGGCGACTTCAGTCTCGACGAGATCGCGCGCCGGCTCGACAA
ACCGATCGAAGAGGTTCGCGCCATCCTCGCGTTGAGCGAACACACCGCCTCGCTCGATGCACCGCTCGACATCGACCCAA
CGCTGTCAATCGGCGAGTCGCTCGCGGATGAGCATGCCGAGACGCCCGAGTCGCAGATCCACAACGCTGAAATCGAAGGC
TTGATCCGGGCCTGGATCGACATGCTCAATGACAAGCAGCGTTTGGTGATCCGCCACCGCTACGGCATTGGCGAGTGCGA
GTTGATGACGCTCGAGGAACTCGCCGCGCGGCTCGAACTGACCCGCGAACGGGTCCGCCAGATCCAGCTCGAGGCGCTGG
GGCAACTGCGCCGCATACTCAAGCGGCGCGGCATTTCGCGCGACGTGCTGTTCTAG

Upstream 100 bases:

>100_bases
AGTACCGATGCAGACCGGCCCAAGCTGCATTTCGAGATTCGCAAGCAGGGGCGGCCGGTGGATCCGATGAAGTTCCTGTC
TGCACGCTGAGAGGAGGCGT

Downstream 100 bases:

>100_bases
CCGCGCTGCAGCGCGGTGCGCTGCAAGCGCTCCCGGCGCAGGGTTTCAGCCAGTTCGAACACCGACATCGCATAGAAACT
GCTGCGGTTGTAGCGGGTGA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MEDPVSSDELESHEPDLPLEVEVFAEPHAPVFENDFLSDVTQIYLNEIGANPLLSAEEEAALARRVRAGDFDARQTMIER
NLRLVVNIAKHYLNRGIPLLDLVEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQ
VLRAQRVVEAASNGDFSLDEIARRLDKPIEEVRAILALSEHTASLDAPLDIDPTLSIGESLADEHAETPESQIHNAEIEG
LIRAWIDMLNDKQRLVIRHRYGIGECELMTLEELAARLELTRERVRQIQLEALGQLRRILKRRGISRDVLF

Sequences:

>Translated_311_residues
MEDPVSSDELESHEPDLPLEVEVFAEPHAPVFENDFLSDVTQIYLNEIGANPLLSAEEEAALARRVRAGDFDARQTMIER
NLRLVVNIAKHYLNRGIPLLDLVEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQ
VLRAQRVVEAASNGDFSLDEIARRLDKPIEEVRAILALSEHTASLDAPLDIDPTLSIGESLADEHAETPESQIHNAEIEG
LIRAWIDMLNDKQRLVIRHRYGIGECELMTLEELAARLELTRERVRQIQLEALGQLRRILKRRGISRDVLF
>Mature_311_residues
MEDPVSSDELESHEPDLPLEVEVFAEPHAPVFENDFLSDVTQIYLNEIGANPLLSAEEEAALARRVRAGDFDARQTMIER
NLRLVVNIAKHYLNRGIPLLDLVEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQ
VLRAQRVVEAASNGDFSLDEIARRLDKPIEEVRAILALSEHTASLDAPLDIDPTLSIGESLADEHAETPESQIHNAEIEG
LIRAWIDMLNDKQRLVIRHRYGIGECELMTLEELAARLELTRERVRQIQLEALGQLRRILKRRGISRDVLF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=54.2124542124542, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI1789448, Length=259, Percent_Identity=38.996138996139, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=30.4347826086957, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35584; Mature: 35584

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDPVSSDELESHEPDLPLEVEVFAEPHAPVFENDFLSDVTQIYLNEIGANPLLSAEEEA
CCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALARRVRAGDFDARQTMIERNLRLVVNIAKHYLNRGIPLLDLVEEGNLGLMHALEKFDPE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCC
RGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQVLRAQRVVEAASNGDFSLDE
CCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IARRLDKPIEEVRAILALSEHTASLDAPLDIDPTLSIGESLADEHAETPESQIHNAEIEG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
LIRAWIDMLNDKQRLVIRHRYGIGECELMTLEELAARLELTRERVRQIQLEALGQLRRIL
HHHHHHHHHCCCHHEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRRGISRDVLF
HHCCCCCCCCC
>Mature Secondary Structure
MEDPVSSDELESHEPDLPLEVEVFAEPHAPVFENDFLSDVTQIYLNEIGANPLLSAEEEA
CCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALARRVRAGDFDARQTMIERNLRLVVNIAKHYLNRGIPLLDLVEEGNLGLMHALEKFDPE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCC
RGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQVLRAQRVVEAASNGDFSLDE
CCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IARRLDKPIEEVRAILALSEHTASLDAPLDIDPTLSIGESLADEHAETPESQIHNAEIEG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
LIRAWIDMLNDKQRLVIRHRYGIGECELMTLEELAARLELTRERVRQIQLEALGQLRRIL
HHHHHHHHHCCCHHEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRRGISRDVLF
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11447163; 12704232 [H]