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The map label for this gene is metZ [H]

Identifier: 119897345

GI number: 119897345

Start: 1133594

End: 1134763

Strand: Direct

Name: metZ [H]

Synonym: azo1054

Alternate gene names: 119897345

Gene position: 1133594-1134763 (Clockwise)

Preceding gene: 119897344

Following gene: 119897351

Centisome position: 25.9

GC content: 67.61

Gene sequence:

>1170_bases
ATGGACGAATTCGACTTCGATACGCTGGCGGTGCGGGCGGGCATAGAGCGTAGCCAGTTCAACGAGCACAGCGAGGCGCT
GTACCTGACCTCGAGCTTCGTGTTCAAGAGCGCGGCGCAGGCGGCGGCCCGTTTTTCCGGCGAAGAAGAGGGCAATGTCT
ACGCCCGCTTCTCCAACCCCACCGTCACCGCGATGCAGACCCGGCTGGCAGCACTGGAAGGGGCGGAAGCCTGCGTGGCG
ACCGCGTCCGGCATGTCGGCGATCCTGTCGCTGGTGATGGCGACCTTGCAGGCGGGCGATCACATCGTCGCCTCCAACGG
CCTGTTCGGGGCAACCCAGCAACTGCTGGGCGGCATCATGAGCAAGTTCGGAATCGAGACCACGTTCGTGCCGGCGACCG
ACATCGCCGCATACCGCGCTGCGGTCCGTCCGCGCACGCGGTTGTTCTTCATCGAAACGCCGTCCAACCCGCTCACCGAG
GTGGTCGACATAGCGGCGGTGGCTGCGGTGGCGCACGAAGCGGGCGCGCTGCTGGCGGTGGACAACTGCTTCTGTACCCC
CGCGCTGCAGCGGCCGCTCGCACTCGGCGCGGATGTCGTCGTGCATTCGGCGACCAAGTATCTCGACGGCCAGGGCCGGG
TGCTGGGCGGCGCGGTGGCCGGTGCCCGTGCGGTGACCGACGAGGTGTTCAAGTTCCTGCGTACCGCGGGGCCGACGCTG
TCGCCGTTCAACGCCTGGGTGATCCTGAAGGGCCTGGAGACGCTGCGCGTGCGCATGGAGGCGCAGTCGGCCACTGCGCT
CGAACTCGCGCGCTGGCTGGAGCAACAGCCTGGCGTCGCCCGTGTTTACTACCCGGGCCTGCCTTCGCATCCTCAGCACG
AACTGGCTATGCGCCAGCAGAAAACCGGCGGCGCCATCCTCGCCTTCGACGTGGAGGGCGGACGCGACGCCGCGTGGCGT
GTGGTGGATGCGACGCGCATGATCTCCATCACCGCCAATCTGGGCGACACCAAGACCACCATCACCCATCCTGCTACCAC
CACCCACGGCCGCGTGTCTGCCGAAGCGCGCAAGGCGGCGGGAATTGGCGATGGTCTGTTGCGGGTGGCGGTGGGGCTGG
AGTCGATCGAGGACCTCAAGGCCGACCTCGCACGGGGTCTGGCGACCTAG

Upstream 100 bases:

>100_bases
GAGAACCAGCGCGGCGAGGAGAAACCCGCCGGGCGTGACGATGGCGAGGAAGGGCAACTCGATCTCAATCTCATCACCCA
TCCGGACCACTGACGCGGTG

Downstream 100 bases:

>100_bases
GTCCGCGGAGATTCGTCGCCCCGCACACAGCGCCGGCCGGTCCGGCGCTTTTTTTTTGTTCGGGGCGCGCCGCTGGTCAG
GCGGCGGGAGTCACAGGCTG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDEFDFDTLAVRAGIERSQFNEHSEALYLTSSFVFKSAAQAAARFSGEEEGNVYARFSNPTVTAMQTRLAALEGAEACVA
TASGMSAILSLVMATLQAGDHIVASNGLFGATQQLLGGIMSKFGIETTFVPATDIAAYRAAVRPRTRLFFIETPSNPLTE
VVDIAAVAAVAHEAGALLAVDNCFCTPALQRPLALGADVVVHSATKYLDGQGRVLGGAVAGARAVTDEVFKFLRTAGPTL
SPFNAWVILKGLETLRVRMEAQSATALELARWLEQQPGVARVYYPGLPSHPQHELAMRQQKTGGAILAFDVEGGRDAAWR
VVDATRMISITANLGDTKTTITHPATTTHGRVSAEARKAAGIGDGLLRVAVGLESIEDLKADLARGLAT

Sequences:

>Translated_389_residues
MDEFDFDTLAVRAGIERSQFNEHSEALYLTSSFVFKSAAQAAARFSGEEEGNVYARFSNPTVTAMQTRLAALEGAEACVA
TASGMSAILSLVMATLQAGDHIVASNGLFGATQQLLGGIMSKFGIETTFVPATDIAAYRAAVRPRTRLFFIETPSNPLTE
VVDIAAVAAVAHEAGALLAVDNCFCTPALQRPLALGADVVVHSATKYLDGQGRVLGGAVAGARAVTDEVFKFLRTAGPTL
SPFNAWVILKGLETLRVRMEAQSATALELARWLEQQPGVARVYYPGLPSHPQHELAMRQQKTGGAILAFDVEGGRDAAWR
VVDATRMISITANLGDTKTTITHPATTTHGRVSAEARKAAGIGDGLLRVAVGLESIEDLKADLARGLAT
>Mature_389_residues
MDEFDFDTLAVRAGIERSQFNEHSEALYLTSSFVFKSAAQAAARFSGEEEGNVYARFSNPTVTAMQTRLAALEGAEACVA
TASGMSAILSLVMATLQAGDHIVASNGLFGATQQLLGGIMSKFGIETTFVPATDIAAYRAAVRPRTRLFFIETPSNPLTE
VVDIAAVAAVAHEAGALLAVDNCFCTPALQRPLALGADVVVHSATKYLDGQGRVLGGAVAGARAVTDEVFKFLRTAGPTL
SPFNAWVILKGLETLRVRMEAQSATALELARWLEQQPGVARVYYPGLPSHPQHELAMRQQKTGGAILAFDVEGGRDAAWR
VVDATRMISITANLGDTKTTITHPATTTHGRVSAEARKAAGIGDGLLRVAVGLESIEDLKADLARGLAT

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=369, Percent_Identity=35.7723577235772, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI299473759, Length=369, Percent_Identity=32.7913279132791, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI25453487, Length=368, Percent_Identity=30.4347826086957, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=38.4816753926702, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=27.6381909547739, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=373, Percent_Identity=36.7292225201072, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17560056, Length=373, Percent_Identity=36.9973190348526, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI71980708, Length=395, Percent_Identity=32.6582278481013, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=33.5897435897436, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=32.7944572748268, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=27.5434243176179, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=315, Percent_Identity=26.984126984127, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=346, Percent_Identity=26.3005780346821, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=35.5844155844156, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 41115; Mature: 41115

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00630 IMP_2 ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEFDFDTLAVRAGIERSQFNEHSEALYLTSSFVFKSAAQAAARFSGEEEGNVYARFSNP
CCCCCCHHHHHHHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
TVTAMQTRLAALEGAEACVATASGMSAILSLVMATLQAGDHIVASNGLFGATQQLLGGIM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
SKFGIETTFVPATDIAAYRAAVRPRTRLFFIETPSNPLTEVVDIAAVAAVAHEAGALLAV
HHHCCCEEECCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEE
DNCFCTPALQRPLALGADVVVHSATKYLDGQGRVLGGAVAGARAVTDEVFKFLRTAGPTL
CCCCCCHHHHCCHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
SPFNAWVILKGLETLRVRMEAQSATALELARWLEQQPGVARVYYPGLPSHPQHELAMRQQ
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
KTGGAILAFDVEGGRDAAWRVVDATRMISITANLGDTKTTITHPATTTHGRVSAEARKAA
CCCCEEEEEECCCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHC
GIGDGLLRVAVGLESIEDLKADLARGLAT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDEFDFDTLAVRAGIERSQFNEHSEALYLTSSFVFKSAAQAAARFSGEEEGNVYARFSNP
CCCCCCHHHHHHHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
TVTAMQTRLAALEGAEACVATASGMSAILSLVMATLQAGDHIVASNGLFGATQQLLGGIM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
SKFGIETTFVPATDIAAYRAAVRPRTRLFFIETPSNPLTEVVDIAAVAAVAHEAGALLAV
HHHCCCEEECCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEE
DNCFCTPALQRPLALGADVVVHSATKYLDGQGRVLGGAVAGARAVTDEVFKFLRTAGPTL
CCCCCCHHHHCCHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
SPFNAWVILKGLETLRVRMEAQSATALELARWLEQQPGVARVYYPGLPSHPQHELAMRQQ
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
KTGGAILAFDVEGGRDAAWRVVDATRMISITANLGDTKTTITHPATTTHGRVSAEARKAA
CCCCEEEEEECCCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHC
GIGDGLLRVAVGLESIEDLKADLARGLAT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]