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The map label for this gene is purF [H]

Identifier: 119897344

GI number: 119897344

Start: 1132057

End: 1133586

Strand: Direct

Name: purF [H]

Synonym: azo1053

Alternate gene names: 119897344

Gene position: 1132057-1133586 (Clockwise)

Preceding gene: 119897343

Following gene: 119897345

Centisome position: 25.87

GC content: 64.58

Gene sequence:

>1530_bases
ATGTGCGGAATTCTTGGGGTGGTTGCGACCTCCCCGGTCAATCAGTTGCTTTACGACGGGCTCCAGGTGCTGCAGCACCG
CGGACAGGATGCCGCAGGCATCGCCACGGCGGAGGGCGGTCGTTTTCACATGCACAAGGGGTCCGGTCTGGTGCGCGACG
TGTTCCGCACCCGCAACATGCGCAACCTCGAAGGCAACTGGGGTATCGCGCACGTACGCTACCCCACCGCAGGGTCGGCC
TATAACGCGGCGGAGGCCCAGCCGTTCTACGTGAATTCGCCGTTCGGACTGCTGCTCGCGCACAACGGCAACCTGACGAA
TTCCGAAGAACTCAAGCGCGAGATGTTCCTCGCCGACTTGCGCCACATCAACACCAATTCCGACTCCGAAGTGCTGCTGA
ACGTGCTCGCGCACGAGCTGCAGGCCGCCTGTAACGGCCTCAAGCTCGATGAGGACGCCGTGTTCCGCGCCGTGGCCGGA
GTGCACCGCCGCTGTCGCGGCGCCTATGCGGCGGTGGTGATGATCGCCGGCTACGGCCTGCTCGCCTTCCGCGACCCCTA
CGGCATCCGCCCGCTGGTGATCGGCCGCAACGATGTCGCCGAGGGCACCGAGTGGCTGGTGGCGTCGGAATCGGTGGCAC
TCGACGTGCTCGGCTTCACGCTGCTGCGCGACGTGGCGCCGGGCGAGGCGGTGCTGATCGACACCCAAGGCAATTTCCGC
AGCCGCCAGTGCGCGGAAAAGACCGTCGAAGCGCCGTGCATGTTCGAGTTCGTCTATCTCGCCCGCCCCGACTCCATCAT
CGACGGCATTTCGGTGTACGAATCGCGCGTCAAGATGGGCGAGTTTCTCGCCGAGAAGATGAAGCGGACGATGCCGCACG
CACAGATCGACGTGGTGATTCCGATCCCCGATTCCAGCCGCCCGTCGGCCATGGAGATGGCGCATCGACTGGGCCTGCCG
TACCGCGAAGGCTTCGTGAAGAACCGCTACATCGGCCGCACCTTCATCATGCCGGGCCAGGCCAAGCGCCGTAAGTCGGT
GCGCCAGAAGCTGAACACGATCCACCAGGAGTTCAAGGGCAAGAGCGTGCTGCTGGTGGACGACTCCATCGTGCGCGGCA
CGACCAGCCGGGAAATCATCAACATGGCGCGCGAAGCCGGCGCCACCAAGGTCTACATGGCCTCCGCTGCGCCGCCGGTG
CGCCACGCCAACGTCTATGGCATCGACATGCCGACGCGCAGCGAACTGATCGCCTCCGACCGCAGCGAAGACGAAATCTG
CCGCGAGATCGGAGCCGACGGTCTGATCTACCAGGACCTCGACGACCTCAAGGCCTCGGTGCGCGCGCTCAATCCCGCGA
TCACCTTCTTCGAGACCTCCTGTTTCGACGGTTGCTACATCACCGGCGACATCACGCCCGAGTACCTCAACGGCGTCGAG
AACCAGCGCGGCGAGGAGAAACCCGCCGGGCGTGACGATGGCGAGGAAGGGCAACTCGATCTCAATCTCATCACCCATCC
GGACCACTGA

Upstream 100 bases:

>100_bases
CGACGTTCTCGCCACCGCTCGAAACGGCGGTGATAGCCGCCAAACCGTGGCTGCCGGAAGCGGTGGCCGCACGAATCCGA
TTCAGATAGGCAGGGCTTCC

Downstream 100 bases:

>100_bases
CGCGGTGATGGACGAATTCGACTTCGATACGCTGGCGGTGCGGGCGGGCATAGAGCGTAGCCAGTTCAACGAGCACAGCG
AGGCGCTGTACCTGACCTCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHMHKGSGLVRDVFRTRNMRNLEGNWGIAHVRYPTAGSA
YNAAEAQPFYVNSPFGLLLAHNGNLTNSEELKREMFLADLRHINTNSDSEVLLNVLAHELQAACNGLKLDEDAVFRAVAG
VHRRCRGAYAAVVMIAGYGLLAFRDPYGIRPLVIGRNDVAEGTEWLVASESVALDVLGFTLLRDVAPGEAVLIDTQGNFR
SRQCAEKTVEAPCMFEFVYLARPDSIIDGISVYESRVKMGEFLAEKMKRTMPHAQIDVVIPIPDSSRPSAMEMAHRLGLP
YREGFVKNRYIGRTFIMPGQAKRRKSVRQKLNTIHQEFKGKSVLLVDDSIVRGTTSREIINMAREAGATKVYMASAAPPV
RHANVYGIDMPTRSELIASDRSEDEICREIGADGLIYQDLDDLKASVRALNPAITFFETSCFDGCYITGDITPEYLNGVE
NQRGEEKPAGRDDGEEGQLDLNLITHPDH

Sequences:

>Translated_509_residues
MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHMHKGSGLVRDVFRTRNMRNLEGNWGIAHVRYPTAGSA
YNAAEAQPFYVNSPFGLLLAHNGNLTNSEELKREMFLADLRHINTNSDSEVLLNVLAHELQAACNGLKLDEDAVFRAVAG
VHRRCRGAYAAVVMIAGYGLLAFRDPYGIRPLVIGRNDVAEGTEWLVASESVALDVLGFTLLRDVAPGEAVLIDTQGNFR
SRQCAEKTVEAPCMFEFVYLARPDSIIDGISVYESRVKMGEFLAEKMKRTMPHAQIDVVIPIPDSSRPSAMEMAHRLGLP
YREGFVKNRYIGRTFIMPGQAKRRKSVRQKLNTIHQEFKGKSVLLVDDSIVRGTTSREIINMAREAGATKVYMASAAPPV
RHANVYGIDMPTRSELIASDRSEDEICREIGADGLIYQDLDDLKASVRALNPAITFFETSCFDGCYITGDITPEYLNGVE
NQRGEEKPAGRDDGEEGQLDLNLITHPDH
>Mature_509_residues
MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHMHKGSGLVRDVFRTRNMRNLEGNWGIAHVRYPTAGSA
YNAAEAQPFYVNSPFGLLLAHNGNLTNSEELKREMFLADLRHINTNSDSEVLLNVLAHELQAACNGLKLDEDAVFRAVAG
VHRRCRGAYAAVVMIAGYGLLAFRDPYGIRPLVIGRNDVAEGTEWLVASESVALDVLGFTLLRDVAPGEAVLIDTQGNFR
SRQCAEKTVEAPCMFEFVYLARPDSIIDGISVYESRVKMGEFLAEKMKRTMPHAQIDVVIPIPDSSRPSAMEMAHRLGLP
YREGFVKNRYIGRTFIMPGQAKRRKSVRQKLNTIHQEFKGKSVLLVDDSIVRGTTSREIINMAREAGATKVYMASAAPPV
RHANVYGIDMPTRSELIASDRSEDEICREIGADGLIYQDLDDLKASVRALNPAITFFETSCFDGCYITGDITPEYLNGVE
NQRGEEKPAGRDDGEEGQLDLNLITHPDH

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.6315789473684, Blast_Score=272, Evalue=7e-73,
Organism=Escherichia coli, GI1788651, Length=504, Percent_Identity=59.1269841269841, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1790167, Length=217, Percent_Identity=32.7188940092166, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=35.1239669421488, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=517, Percent_Identity=51.063829787234, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24659598, Length=505, Percent_Identity=36.4356435643564, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24659604, Length=481, Percent_Identity=37.4220374220374, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28573187, Length=472, Percent_Identity=36.228813559322, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56126; Mature: 56126

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHMHKGSGLVRDVFRTRNM
CCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RNLEGNWGIAHVRYPTAGSAYNAAEAQPFYVNSPFGLLLAHNGNLTNSEELKREMFLADL
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVLAHELQAACNGLKLDEDAVFRAVAGVHRRCRGAYAAVVMIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPYGIRPLVIGRNDVAEGTEWLVASESVALDVLGFTLLRDVAPGEAVLIDTQGNFR
EEECCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SRQCAEKTVEAPCMFEFVYLARPDSIIDGISVYESRVKMGEFLAEKMKRTMPHAQIDVVI
HHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PIPDSSRPSAMEMAHRLGLPYREGFVKNRYIGRTFIMPGQAKRRKSVRQKLNTIHQEFKG
ECCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
KSVLLVDDSIVRGTTSREIINMAREAGATKVYMASAAPPVRHANVYGIDMPTRSELIASD
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHCC
RSEDEICREIGADGLIYQDLDDLKASVRALNPAITFFETSCFDGCYITGDITPEYLNGVE
CCHHHHHHHHCCCCCEECCHHHHHHHHHHHCCHHEEEECCCCCCEEEECCCCHHHHCCHH
NQRGEEKPAGRDDGEEGQLDLNLITHPDH
HCCCCCCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHMHKGSGLVRDVFRTRNM
CCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RNLEGNWGIAHVRYPTAGSAYNAAEAQPFYVNSPFGLLLAHNGNLTNSEELKREMFLADL
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVLAHELQAACNGLKLDEDAVFRAVAGVHRRCRGAYAAVVMIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPYGIRPLVIGRNDVAEGTEWLVASESVALDVLGFTLLRDVAPGEAVLIDTQGNFR
EEECCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SRQCAEKTVEAPCMFEFVYLARPDSIIDGISVYESRVKMGEFLAEKMKRTMPHAQIDVVI
HHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
PIPDSSRPSAMEMAHRLGLPYREGFVKNRYIGRTFIMPGQAKRRKSVRQKLNTIHQEFKG
ECCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
KSVLLVDDSIVRGTTSREIINMAREAGATKVYMASAAPPVRHANVYGIDMPTRSELIASD
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHCC
RSEDEICREIGADGLIYQDLDDLKASVRALNPAITFFETSCFDGCYITGDITPEYLNGVE
CCHHHHHHHHCCCCCEECCHHHHHHHHHHHCCHHEEEECCCCCCEEEECCCCHHHHCCHH
NQRGEEKPAGRDDGEEGQLDLNLITHPDH
HCCCCCCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]