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The map label for this gene is prfA

Identifier: 119897272

GI number: 119897272

Start: 1054469

End: 1055554

Strand: Direct

Name: prfA

Synonym: azo0981

Alternate gene names: 119897272

Gene position: 1054469-1055554 (Clockwise)

Preceding gene: 119897271

Following gene: 119897273

Centisome position: 24.1

GC content: 67.31

Gene sequence:

>1086_bases
ATGAAACAGAGTATCCGGGACAAACTCGAGCATCTGGCCGGCCGCCTTGCGGAGCTGGATCGCGAGCTTTCGTCCGAAGA
CGCCACGCGCGACATGAACGCCTTCCGCGACTTGTCGCGGGAGCGCGCCGAACTTGAGCCCGTCGTCGCGCTGTACGGGG
CGTATCGACAAGCGGAGCAGGACTGCGCGACGGCGCGTGAACTGCTCGACGACCCCGAGATGCAGGAACTGGGTCGCAGC
GAACTCGAAGCGGGCGAGGCCCGCATCGCCGCGCTCGACGGCGACCTGCAGCGCGCCTTGCTGCCCCGCGACCCGAACGA
CGAGCGCAACCTTTTCCTGGAAATTCGCGCCGGTACCGGCGGGGACGAAGCAGCGTTGTTCGCCGGCGACCTGCTGCGCA
TGTATTCGCGCTACGCCGAACGCCAGCGCTGGCGGGTGGAGATCGTGTCGTCCAATCCGTCCGACCTCGGCGGCTACAAG
GAGGTCATCGCCCGCATCGTCGGCGCCGGCGCCTATTCGCGCCTCAAGTTCGAATCCGGCGGGCATCGGGTGCAGCGTAT
TCCGGTTACCGAGACGCAGGGACGCATCCACACCTCGGCGTGCACCGTGGCGGTGATGCCCGAGGCGGACGAGCTTGCAG
CGGTGGAAATCAACCCCGCCGACCTTCGCATCGACACTTTCCGGGCGTCGGGCGCGGGCGGACAGCACATCAACAAGACT
GATTCCGCGGTCCGCATCACCCATCTCCCCACCGGTCTGGTCGTGGAGTGCCAGGACGACCGCTCCCAGCACCGCAACAA
GGCGCAGGCCATGGCGGTGCTGGCTGCGCGACTGAACGATGCGCAGCTGCGCGCGCGTCAGAGTGCGGAGGCGGCCACCC
GGCGCAGCCTGATCGGCAGTGGCGACCGTTCCGAGCGTATCCGCACCTACAACTTTCCCCAAGGGCGGGTCACCGACCAC
CGCATCAACCTCACCTTGTACAAGCTCGACGCGGTCATGCAGGGCGAACTCGACGAACTGCTCGCGGCCCTCGTGCTGGA
GCATCAGGCCGAGCAGCTCGCGGCACTCGCCGAGGAGGGGCTGTGA

Upstream 100 bases:

>100_bases
AAGGGGAAGGGCAGGCCGAGGCCAGCCGGGTGGTGCAGCAACTGTTCAATCTTTCGCGCCACGACTAAGGCGGTGCGCGC
CTGCAGCGCACCTTGTTCTC

Downstream 100 bases:

>100_bases
GCCTGCCGCCGGATATCGCAGGCGCTTTGAGCTGGGCGCGCGAACGGATCGAGCAGGTCGATGCGCGCGTTCTGCTGCGA
TACGCGCTGGCATGCCCCGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKQSIRDKLEHLAGRLAELDRELSSEDATRDMNAFRDLSRERAELEPVVALYGAYRQAEQDCATARELLDDPEMQELGRS
ELEAGEARIAALDGDLQRALLPRDPNDERNLFLEIRAGTGGDEAALFAGDLLRMYSRYAERQRWRVEIVSSNPSDLGGYK
EVIARIVGAGAYSRLKFESGGHRVQRIPVTETQGRIHTSACTVAVMPEADELAAVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNKAQAMAVLAARLNDAQLRARQSAEAATRRSLIGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLDAVMQGELDELLAALVLEHQAEQLAALAEEGL

Sequences:

>Translated_361_residues
MKQSIRDKLEHLAGRLAELDRELSSEDATRDMNAFRDLSRERAELEPVVALYGAYRQAEQDCATARELLDDPEMQELGRS
ELEAGEARIAALDGDLQRALLPRDPNDERNLFLEIRAGTGGDEAALFAGDLLRMYSRYAERQRWRVEIVSSNPSDLGGYK
EVIARIVGAGAYSRLKFESGGHRVQRIPVTETQGRIHTSACTVAVMPEADELAAVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNKAQAMAVLAARLNDAQLRARQSAEAATRRSLIGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLDAVMQGELDELLAALVLEHQAEQLAALAEEGL
>Mature_361_residues
MKQSIRDKLEHLAGRLAELDRELSSEDATRDMNAFRDLSRERAELEPVVALYGAYRQAEQDCATARELLDDPEMQELGRS
ELEAGEARIAALDGDLQRALLPRDPNDERNLFLEIRAGTGGDEAALFAGDLLRMYSRYAERQRWRVEIVSSNPSDLGGYK
EVIARIVGAGAYSRLKFESGGHRVQRIPVTETQGRIHTSACTVAVMPEADELAAVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNKAQAMAVLAARLNDAQLRARQSAEAATRRSLIGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLDAVMQGELDELLAALVLEHQAEQLAALAEEGL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=48.5342019543974, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI34577120, Length=339, Percent_Identity=41.2979351032448, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI166795305, Length=216, Percent_Identity=46.2962962962963, Blast_Score=187, Evalue=9e-48,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=63.5097493036212, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.9221556886228, Blast_Score=218, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=266, Percent_Identity=37.218045112782, Blast_Score=180, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=345, Percent_Identity=40, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=42.5, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_AZOSB (A1K443)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932485.1
- ProteinModelPortal:   A1K443
- SMR:   A1K443
- STRING:   A1K443
- GeneID:   4606654
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0981
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   A1K443
- ProtClustDB:   PRK00591
- BioCyc:   ASP62928:AZO0981-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39978; Mature: 39978

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSIRDKLEHLAGRLAELDRELSSEDATRDMNAFRDLSRERAELEPVVALYGAYRQAEQ
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DCATARELLDDPEMQELGRSELEAGEARIAALDGDLQRALLPRDPNDERNLFLEIRAGTG
HHHHHHHHHCCCCHHHHCHHHHCCCCEEEEEECCHHHHHCCCCCCCCCCCEEEEEECCCC
GDEAALFAGDLLRMYSRYAERQRWRVEIVSSNPSDLGGYKEVIARIVGAGAYSRLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRIPVTETQGRIHTSACTVAVMPEADELAAVEINPADLRIDTFRASGAGGQHINKT
CCEEEEECCCCCCCCEEECEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVECQDDRSQHRNKAQAMAVLAARLNDAQLRARQSAEAATRRSLIGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLDAVMQGELDELLAALVLEHQAEQLAALAEEG
CCCCCCCEEECCCCCCCCCEEEEEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
L
C
>Mature Secondary Structure
MKQSIRDKLEHLAGRLAELDRELSSEDATRDMNAFRDLSRERAELEPVVALYGAYRQAEQ
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DCATARELLDDPEMQELGRSELEAGEARIAALDGDLQRALLPRDPNDERNLFLEIRAGTG
HHHHHHHHHCCCCHHHHCHHHHCCCCEEEEEECCHHHHHCCCCCCCCCCCEEEEEECCCC
GDEAALFAGDLLRMYSRYAERQRWRVEIVSSNPSDLGGYKEVIARIVGAGAYSRLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRIPVTETQGRIHTSACTVAVMPEADELAAVEINPADLRIDTFRASGAGGQHINKT
CCEEEEECCCCCCCCEEECEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVECQDDRSQHRNKAQAMAVLAARLNDAQLRARQSAEAATRRSLIGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLDAVMQGELDELLAALVLEHQAEQLAALAEEG
CCCCCCCEEECCCCCCCCCEEEEEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA