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The map label for this gene is hemK [H]

Identifier: 119897273

GI number: 119897273

Start: 1055551

End: 1056387

Strand: Direct

Name: hemK [H]

Synonym: azo0982

Alternate gene names: 119897273

Gene position: 1055551-1056387 (Clockwise)

Preceding gene: 119897272

Following gene: 119897274

Centisome position: 24.12

GC content: 67.03

Gene sequence:

>837_bases
GTGAGCCTGCCGCCGGATATCGCAGGCGCTTTGAGCTGGGCGCGCGAACGGATCGAGCAGGTCGATGCGCGCGTTCTGCT
GCGATACGCGCTGGCATGCCCCGCGTCCCGCCTCGTCGCATGGCCGGAGCAACGCTTGAGTGCGGAGGAATGGGAGAACT
TCTCCCGCCTGGTGGAGCGGCGGGTGGAAGGCGAGCCGGTCGCCTACCTCATCGGCGAACGCGAGTTCTACGGGCGCAGT
TTTGCCGTGACGCCCGCTGTCCTGATCCCCCGCCCCGATACCGAGCTGCTGGTCGAGCTTGCGGTGGCGCATTTTTCCGC
GCAGCAGCGCGTACGGGTGCTGGACCTTGGCACCGGCAGTGGCGCCCTCGCCATCACGCTGGCGCTGGAACTGGATGCGG
CGGACGTGACCGCGCTGGACCGTTCTCGCGAGGCTTTATGGGTGGCGATGGCAAACGCGGCCCGGCTGGGCGCCAGTGTT
TCCTTCGTGCAGAGCGACTGGTTCGACGCGCTGGGGGAAGAGCGCTTTCAGCTAATTGTCGCAAACCCCCCCTACATCGC
GGCGGACGACGCTCATCTGGAAGAGGGCGATCTGAGATTCGAACCGCGTTCCGCGCTGGCTGCCGGCACGGCCGGGCTGG
ATGATCTGAGCGAGATTGCGGCCGCTGCCCCGGCGCATCTCGAGGCCGGCGGCTGGCTTTTCCTGGAGCACGGCTACGAT
CAGGCCATGTCGGTACGCGGTTTGCTTGCAGATGCCGGCTTCGCCGCCATTTCCTCCTGGAAGGATCTGGCAGGGATCGA
GCGGGTTACCGGGGGGCGTTGGTTGGGGCGTGACTGA

Upstream 100 bases:

>100_bases
AGCTCGACGCGGTCATGCAGGGCGAACTCGACGAACTGCTCGCGGCCCTCGTGCTGGAGCATCAGGCCGAGCAGCTCGCG
GCACTCGCCGAGGAGGGGCT

Downstream 100 bases:

>100_bases
CCGCGCCTGTCCCGTATTGACGCTTGCCCCGGGCAGGCGCTAGACTTTTCCGACTGTTTTACTCAACTTTTTCGAGAGTC
ATAAGCATGGACATCAAGGA

Product: HemK protein

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSLPPDIAGALSWARERIEQVDARVLLRYALACPASRLVAWPEQRLSAEEWENFSRLVERRVEGEPVAYLIGEREFYGRS
FAVTPAVLIPRPDTELLVELAVAHFSAQQRVRVLDLGTGSGALAITLALELDAADVTALDRSREALWVAMANAARLGASV
SFVQSDWFDALGEERFQLIVANPPYIAADDAHLEEGDLRFEPRSALAAGTAGLDDLSEIAAAAPAHLEAGGWLFLEHGYD
QAMSVRGLLADAGFAAISSWKDLAGIERVTGGRWLGRD

Sequences:

>Translated_278_residues
MSLPPDIAGALSWARERIEQVDARVLLRYALACPASRLVAWPEQRLSAEEWENFSRLVERRVEGEPVAYLIGEREFYGRS
FAVTPAVLIPRPDTELLVELAVAHFSAQQRVRVLDLGTGSGALAITLALELDAADVTALDRSREALWVAMANAARLGASV
SFVQSDWFDALGEERFQLIVANPPYIAADDAHLEEGDLRFEPRSALAAGTAGLDDLSEIAAAAPAHLEAGGWLFLEHGYD
QAMSVRGLLADAGFAAISSWKDLAGIERVTGGRWLGRD
>Mature_277_residues
SLPPDIAGALSWARERIEQVDARVLLRYALACPASRLVAWPEQRLSAEEWENFSRLVERRVEGEPVAYLIGEREFYGRSF
AVTPAVLIPRPDTELLVELAVAHFSAQQRVRVLDLGTGSGALAITLALELDAADVTALDRSREALWVAMANAARLGASVS
FVQSDWFDALGEERFQLIVANPPYIAADDAHLEEGDLRFEPRSALAAGTAGLDDLSEIAAAAPAHLEAGGWLFLEHGYDQ
AMSVRGLLADAGFAAISSWKDLAGIERVTGGRWLGRD

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=238, Percent_Identity=31.5126050420168, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787463, Length=271, Percent_Identity=41.6974169741697, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI87082085, Length=194, Percent_Identity=36.5979381443299, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582226, Length=240, Percent_Identity=27.9166666666667, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30179; Mature: 30048

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPPDIAGALSWARERIEQVDARVLLRYALACPASRLVAWPEQRLSAEEWENFSRLVER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCHHHHHHHHHHHHH
RVEGEPVAYLIGEREFYGRSFAVTPAVLIPRPDTELLVELAVAHFSAQQRVRVLDLGTGS
HCCCCCEEEEECCHHHCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCC
GALAITLALELDAADVTALDRSREALWVAMANAARLGASVSFVQSDWFDALGEERFQLIV
CEEEEEEEEEECCCHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCEEEEEE
ANPPYIAADDAHLEEGDLRFEPRSALAAGTAGLDDLSEIAAAAPAHLEAGGWLFLEHGYD
CCCCEEECCCCCCCCCCCEECCHHHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEECCCC
QAMSVRGLLADAGFAAISSWKDLAGIERVTGGRWLGRD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLPPDIAGALSWARERIEQVDARVLLRYALACPASRLVAWPEQRLSAEEWENFSRLVER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCHHHHHHHHHHHHH
RVEGEPVAYLIGEREFYGRSFAVTPAVLIPRPDTELLVELAVAHFSAQQRVRVLDLGTGS
HCCCCCEEEEECCHHHCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCC
GALAITLALELDAADVTALDRSREALWVAMANAARLGASVSFVQSDWFDALGEERFQLIV
CEEEEEEEEEECCCHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCEEEEEE
ANPPYIAADDAHLEEGDLRFEPRSALAAGTAGLDDLSEIAAAAPAHLEAGGWLFLEHGYD
CCCCEEECCCCCCCCCCCEECCHHHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEECCCC
QAMSVRGLLADAGFAAISSWKDLAGIERVTGGRWLGRD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]