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The map label for this gene is hemA

Identifier: 119897271

GI number: 119897271

Start: 1053183

End: 1054436

Strand: Direct

Name: hemA

Synonym: azo0980

Alternate gene names: 119897271

Gene position: 1053183-1054436 (Clockwise)

Preceding gene: 119897269

Following gene: 119897272

Centisome position: 24.07

GC content: 67.15

Gene sequence:

>1254_bases
ATGCAACTCTACGCCCTCGGCCTGAACCACCATACGGCCCCGCTCGCGATTCGCGAACGGGTGGCGTTTCAGCCCGACCG
GCTTGATCAGGCCCTGCAGGCGCTCACCGATTCGCGCACCGTGAGCGAAGCGGCCATCCTCTCCACCTGTAATCGCACCG
AGCTGTACTTCGCTGCCGAACAACCGCAGCGCGCGGCCGACTGGCTGGCGGGATTTCATCAGCTGCCGCTCGCCCAGGTG
TCGCCCTATCTGTATTCGTATCCGCAGCGGGACGCGGTTCGCCATGTGTTCCGGGTAGCGAGCGGGCTCGACTCCATGGT
GCTGGGCGAACCGCAGATACTCGGGCAGGTGAAAGAGGCTGCCCGGCGCGCCGAAGAGGCGGGCACGCTCGGCACGCTGC
TGCACAAGCTGTTCCAGAACACGTTCGCGGTGGCGAAGGAGGTGCGCTCGACCACCGCGATCGGCGCCAACATCGTCTCG
ATGGCGGCGGCCGCGGTGCATCTGACCGGACGTATTTTCGAGCGGGTTTCGGATCAGCACGTGCTGTTCATCGGCGCGGG
CGAAATGATCGAATTGTGTGCGGCCCACTTCGCCGGCGCGGGGCCGCGCTCGATGACGGTTGCCAACCGCACCGAAGCGC
GCGCCGAGGCGCTGGCGGCGCGGCTCGGTGCGCAAACCATGCGGCTCGACGCCATCGCCGATGCGCTGCCGCGTTTTGAC
GTGGTGGTCTCCTGTACGGCCTCGCCCTTGCCCATCGTGGGCCTGGGTATGGTCGAGCGGGCGGTGAAGGTCCGCCGCCA
CCGGCCGATCGTGATGGTCGATCTTGCCGTTCCGCGCGACGTCGAACCCGAGGTGGGGCAGCTGGACGACGTGTTTCTCT
ACACCGTCGATGATCTCGCGCAGGTGGTCGATGCGGGTATCGAATCGCGCCAGCAGGCGGTGGTGGAGGCAGAGGGCATC
ATCGACCAGCGCGTCGACGGCTTTCTGCACTGGCTGCACGCGCGTGACGCGGTGCCGACCATCCGCGCGTTGCGCGAGCA
CGCCGAAACGCTGCGCCGGGGCGAGATCGAGCGCGCGCTTCGCCAGCTTGCGAAGGGCGAAGACCCGCAGGCGGTGCTCG
ACGCGCTGTCGCACGGGCTCACCAACAAATTGATGCACGGTCCTACCCGCTTCCTGACCCAGGCCGAAGGGGAAGGGCAG
GCCGAGGCCAGCCGGGTGGTGCAGCAACTGTTCAATCTTTCGCGCCACGACTAA

Upstream 100 bases:

>100_bases
TTTTGGCTCGCCCGGCATCCCGAAATCCCTGATTTGGCGGATTTTTTTGGGGGGTGGCCGCGGTGCTATAATGCGCCCAA
CCCGCCAGCCCGCCCCTCGC

Downstream 100 bases:

>100_bases
GGCGGTGCGCGCCTGCAGCGCACCTTGTTCTCATGAAACAGAGTATCCGGGACAAACTCGAGCATCTGGCCGGCCGCCTT
GCGGAGCTGGATCGCGAGCT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQLYALGLNHHTAPLAIRERVAFQPDRLDQALQALTDSRTVSEAAILSTCNRTELYFAAEQPQRAADWLAGFHQLPLAQV
SPYLYSYPQRDAVRHVFRVASGLDSMVLGEPQILGQVKEAARRAEEAGTLGTLLHKLFQNTFAVAKEVRSTTAIGANIVS
MAAAAVHLTGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFD
VVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQVVDAGIESRQQAVVEAEGI
IDQRVDGFLHWLHARDAVPTIRALREHAETLRRGEIERALRQLAKGEDPQAVLDALSHGLTNKLMHGPTRFLTQAEGEGQ
AEASRVVQQLFNLSRHD

Sequences:

>Translated_417_residues
MQLYALGLNHHTAPLAIRERVAFQPDRLDQALQALTDSRTVSEAAILSTCNRTELYFAAEQPQRAADWLAGFHQLPLAQV
SPYLYSYPQRDAVRHVFRVASGLDSMVLGEPQILGQVKEAARRAEEAGTLGTLLHKLFQNTFAVAKEVRSTTAIGANIVS
MAAAAVHLTGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFD
VVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQVVDAGIESRQQAVVEAEGI
IDQRVDGFLHWLHARDAVPTIRALREHAETLRRGEIERALRQLAKGEDPQAVLDALSHGLTNKLMHGPTRFLTQAEGEGQ
AEASRVVQQLFNLSRHD
>Mature_417_residues
MQLYALGLNHHTAPLAIRERVAFQPDRLDQALQALTDSRTVSEAAILSTCNRTELYFAAEQPQRAADWLAGFHQLPLAQV
SPYLYSYPQRDAVRHVFRVASGLDSMVLGEPQILGQVKEAARRAEEAGTLGTLLHKLFQNTFAVAKEVRSTTAIGANIVS
MAAAAVHLTGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFD
VVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQVVDAGIESRQQAVVEAEGI
IDQRVDGFLHWLHARDAVPTIRALREHAETLRRGEIERALRQLAKGEDPQAVLDALSHGLTNKLMHGPTRFLTQAEGEGQ
AEASRVVQQLFNLSRHD

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=417, Percent_Identity=46.7625899280576, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_AZOSB (A1K442)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932484.1
- ProteinModelPortal:   A1K442
- SMR:   A1K442
- STRING:   A1K442
- GeneID:   4610012
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0980
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- PhylomeDB:   A1K442
- ProtClustDB:   PRK00045
- BioCyc:   ASP62928:AZO0980-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 45647; Mature: 45647

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 105-105 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYALGLNHHTAPLAIRERVAFQPDRLDQALQALTDSRTVSEAAILSTCNRTELYFAAE
CEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECC
QPQRAADWLAGFHQLPLAQVSPYLYSYPQRDAVRHVFRVASGLDSMVLGEPQILGQVKEA
CCHHHHHHHHHHHHCCHHHHCHHHHCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHH
ARRAEEAGTLGTLLHKLFQNTFAVAKEVRSTTAIGANIVSMAAAAVHLTGRIFERVSDQH
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFD
EEEEECCHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHEEEHHHHH
QVVDAGIESRQQAVVEAEGIIDQRVDGFLHWLHARDAVPTIRALREHAETLRRGEIERAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RQLAKGEDPQAVLDALSHGLTNKLMHGPTRFLTQAEGEGQAEASRVVQQLFNLSRHD
HHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQLYALGLNHHTAPLAIRERVAFQPDRLDQALQALTDSRTVSEAAILSTCNRTELYFAAE
CEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECC
QPQRAADWLAGFHQLPLAQVSPYLYSYPQRDAVRHVFRVASGLDSMVLGEPQILGQVKEA
CCHHHHHHHHHHHHCCHHHHCHHHHCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHH
ARRAEEAGTLGTLLHKLFQNTFAVAKEVRSTTAIGANIVSMAAAAVHLTGRIFERVSDQH
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFD
EEEEECCHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHEEEHHHHH
QVVDAGIESRQQAVVEAEGIIDQRVDGFLHWLHARDAVPTIRALREHAETLRRGEIERAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RQLAKGEDPQAVLDALSHGLTNKLMHGPTRFLTQAEGEGQAEASRVVQQLFNLSRHD
HHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA