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The map label for this gene is rnfE1 [H]

Identifier: 119896803

GI number: 119896803

Start: 547551

End: 548285

Strand: Reverse

Name: rnfE1 [H]

Synonym: azo0512

Alternate gene names: 119896803

Gene position: 548285-547551 (Counterclockwise)

Preceding gene: 119896804

Following gene: 119896802

Centisome position: 12.53

GC content: 67.21

Gene sequence:

>735_bases
ATGAGCGAAGCCTGCGCAAGCGCGGCCGCCGACGGCGCCGCCAAGCCCGCCCCCAGCGGTCCGTCTTATGCCAGCCTGAT
CAAGGACGGCTTGTGGTCCAACAACCCGGTGCTGGCGCAGATGGTCGCGCTGTGCCCCACCCTGGCGGTGACCAGCAGCG
CGACCAACGGACTCGGCATGGGACTGGCCACCACGGCCGTGCTGATCCTGTCGAACATGCTGATTTCCTCGATCCGCAAC
CTCGTCAGTCCCAATGTGCGCATTCCGGTGTTCGTCGTCGTCATCGCGACGCTGGTCAGCCTGATCGACATGAGCATGAA
CGCCTGGATGTACGAGCTGTACCAGGTGCTGGGCCTGTTCATCGCGCTGATCGTCGTCAACTGCGCCATCCTCGGCCGCG
CCGAGGCCTTCGCCTCCAAGAACACGGTATTGTCGTCTGCGGTGGATGGCCTGGGCATGGGGCTGGGCTTCACCGCCACG
CTGGTGGTGCTCGGCGGCGTCCGCGAAATCCTCGGCAGCGGCACGCTGTTCGCCGGCGCCAGCAACATGCTCGGCAGCCA
GTTCGCGTTTCTCGAACTGACGCTGATCCCCGGCTACAAGGGCTTCCTGATGATGATCCTGCCGCCCGGCGCATTCTTCG
TCATCGGTTGCCTGATCGCCGCCCACCGGGTGCTGTCGCAGCGGGCCGAACGCCGCGCGGCGGCGGCCCCCGGCGCCGAC
ACCGCCCCCGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCGTGGTCGGCGCGGTCAAGCGCGGGCTCGAACTCTACACCGGCAACCGCGAGGCGATGATCGCCGCCGGCGCCCC
CCGATCCTGAGGAGAATGTC

Downstream 100 bases:

>100_bases
TCCGCCACGAGGACCCCATCATGCGCATCGGAGTTGCCTACGCCGACGTCGCCCAGCCGGTCTGGCTGAAGATCGACGTT
CCCGACACCGCCACCGTGCA

Product: electron transport complex subunit RsxE

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSEACASAAADGAAKPAPSGPSYASLIKDGLWSNNPVLAQMVALCPTLAVTSSATNGLGMGLATTAVLILSNMLISSIRN
LVSPNVRIPVFVVVIATLVSLIDMSMNAWMYELYQVLGLFIALIVVNCAILGRAEAFASKNTVLSSAVDGLGMGLGFTAT
LVVLGGVREILGSGTLFAGASNMLGSQFAFLELTLIPGYKGFLMMILPPGAFFVIGCLIAAHRVLSQRAERRAAAAPGAD
TAPA

Sequences:

>Translated_244_residues
MSEACASAAADGAAKPAPSGPSYASLIKDGLWSNNPVLAQMVALCPTLAVTSSATNGLGMGLATTAVLILSNMLISSIRN
LVSPNVRIPVFVVVIATLVSLIDMSMNAWMYELYQVLGLFIALIVVNCAILGRAEAFASKNTVLSSAVDGLGMGLGFTAT
LVVLGGVREILGSGTLFAGASNMLGSQFAFLELTLIPGYKGFLMMILPPGAFFVIGCLIAAHRVLSQRAERRAAAAPGAD
TAPA
>Mature_243_residues
SEACASAAADGAAKPAPSGPSYASLIKDGLWSNNPVLAQMVALCPTLAVTSSATNGLGMGLATTAVLILSNMLISSIRNL
VSPNVRIPVFVVVIATLVSLIDMSMNAWMYELYQVLGLFIALIVVNCAILGRAEAFASKNTVLSSAVDGLGMGLGFTATL
VVLGGVREILGSGTLFAGASNMLGSQFAFLELTLIPGYKGFLMMILPPGAFFVIGCLIAAHRVLSQRAERRAAAAPGADT
APA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=211, Percent_Identity=48.3412322274881, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1787914, Length=142, Percent_Identity=34.5070422535211, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 25008; Mature: 24877

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEACASAAADGAAKPAPSGPSYASLIKDGLWSNNPVLAQMVALCPTLAVTSSATNGLGM
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
GLATTAVLILSNMLISSIRNLVSPNVRIPVFVVVIATLVSLIDMSMNAWMYELYQVLGLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALIVVNCAILGRAEAFASKNTVLSSAVDGLGMGLGFTATLVVLGGVREILGSGTLFAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SNMLGSQFAFLELTLIPGYKGFLMMILPPGAFFVIGCLIAAHRVLSQRAERRAAAAPGAD
HHHHCCCCHHEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TAPA
CCCC
>Mature Secondary Structure 
SEACASAAADGAAKPAPSGPSYASLIKDGLWSNNPVLAQMVALCPTLAVTSSATNGLGM
CHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
GLATTAVLILSNMLISSIRNLVSPNVRIPVFVVVIATLVSLIDMSMNAWMYELYQVLGLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALIVVNCAILGRAEAFASKNTVLSSAVDGLGMGLGFTATLVVLGGVREILGSGTLFAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SNMLGSQFAFLELTLIPGYKGFLMMILPPGAFFVIGCLIAAHRVLSQRAERRAAAAPGAD
HHHHCCCCHHEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TAPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]