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The map label for this gene is rnfG1 [H]

Identifier: 119896804

GI number: 119896804

Start: 548296

End: 548922

Strand: Reverse

Name: rnfG1 [H]

Synonym: azo0513

Alternate gene names: 119896804

Gene position: 548922-548296 (Counterclockwise)

Preceding gene: 119896805

Following gene: 119896803

Centisome position: 12.54

GC content: 70.18

Gene sequence:

>627_bases
ATGGACATCGCCAAGCAGTTCGACACCCTCTGGTACCAGGGCGCCTCGCTCGCCGCGGTCGCCCTGATCACCGGCGCCGC
GCTTGCAGTCGCCTACCAGAGCGCCGCTCCGCGCATCGCCGAGGCGCAGATGCGCAAGACCCAGGCCGCGCTGGCCGAAG
TGCTGCCGCAGGGCAGCTTCGACAACGACCTGATGGCCGACACCGTCACGATCAAGGACGCCGCCGGGCGCGAGGTCACG
GTCTATCGCGCGCGCAAGGCGGGCGCGGTCACCGGCGTCGTGTTCGGGATGGAGAACAAGGGCTATTCCGGCACCATCAA
GCTGGTGATGGGCGTCGATCTCGACGGCCGCCTGACCGGCGTGCGCGTCACCGGCCACACCGAAACCCCCGGGCTGGGCG
ACAAGATCGAACTTGCCAAGACGCCCTGGGTGCTCGGCTTCAACGGCAAGTCGCTGGACGATCCCGCCCCCGCGCTGTGG
GCGGTGAAGAAGGACGGCGGCGTGTTCGACCAGTTCGCCGGCGCCACCATCACCCCGCGCGCCGTGGTCGGCGCGGTCAA
GCGCGGGCTCGAACTCTACACCGGCAACCGCGAGGCGATGATCGCCGCCGGCGCCCCCCGATCCTGA

Upstream 100 bases:

>100_bases
CCGCTGATCGACCAATACACCCGGCCGCGCATCTTCGGCCGCAGCCGCGACGGTCGCGCGCTGAATCCCGCCGAATCCCG
CTGACATCAAGGCGCGCACC

Downstream 100 bases:

>100_bases
GGAGAATGTCATGAGCGAAGCCTGCGCAAGCGCGGCCGCCGACGGCGCCGCCAAGCCCGCCCCCAGCGGTCCGTCTTATG
CCAGCCTGATCAAGGACGGC

Product: electron transport complex protein RnfG

Products: NA

Alternate protein names: Nitrogen fixation protein rnfG [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MDIAKQFDTLWYQGASLAAVALITGAALAVAYQSAAPRIAEAQMRKTQAALAEVLPQGSFDNDLMADTVTIKDAAGREVT
VYRARKAGAVTGVVFGMENKGYSGTIKLVMGVDLDGRLTGVRVTGHTETPGLGDKIELAKTPWVLGFNGKSLDDPAPALW
AVKKDGGVFDQFAGATITPRAVVGAVKRGLELYTGNREAMIAAGAPRS

Sequences:

>Translated_208_residues
MDIAKQFDTLWYQGASLAAVALITGAALAVAYQSAAPRIAEAQMRKTQAALAEVLPQGSFDNDLMADTVTIKDAAGREVT
VYRARKAGAVTGVVFGMENKGYSGTIKLVMGVDLDGRLTGVRVTGHTETPGLGDKIELAKTPWVLGFNGKSLDDPAPALW
AVKKDGGVFDQFAGATITPRAVVGAVKRGLELYTGNREAMIAAGAPRS
>Mature_208_residues
MDIAKQFDTLWYQGASLAAVALITGAALAVAYQSAAPRIAEAQMRKTQAALAEVLPQGSFDNDLMADTVTIKDAAGREVT
VYRARKAGAVTGVVFGMENKGYSGTIKLVMGVDLDGRLTGVRVTGHTETPGLGDKIELAKTPWVLGFNGKSLDDPAPALW
AVKKDGGVFDQFAGATITPRAVVGAVKRGLELYTGNREAMIAAGAPRS

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4659

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfG

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rnfG family [H]

Homologues:

Organism=Escherichia coli, GI1787918, Length=185, Percent_Identity=43.2432432432432, Blast_Score=127, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022989
- InterPro:   IPR010209
- InterPro:   IPR007329 [H]

Pfam domain/function: PF04205 FMN_bind [H]

EC number: NA

Molecular weight: Translated: 21754; Mature: 21754

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAKQFDTLWYQGASLAAVALITGAALAVAYQSAAPRIAEAQMRKTQAALAEVLPQGSF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
DNDLMADTVTIKDAAGREVTVYRARKAGAVTGVVFGMENKGYSGTIKLVMGVDLDGRLTG
CCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEE
VRVTGHTETPGLGDKIELAKTPWVLGFNGKSLDDPAPALWAVKKDGGVFDQFAGATITPR
EEEECCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHCCCEECHH
AVVGAVKRGLELYTGNREAMIAAGAPRS
HHHHHHHCCEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MDIAKQFDTLWYQGASLAAVALITGAALAVAYQSAAPRIAEAQMRKTQAALAEVLPQGSF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
DNDLMADTVTIKDAAGREVTVYRARKAGAVTGVVFGMENKGYSGTIKLVMGVDLDGRLTG
CCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEE
VRVTGHTETPGLGDKIELAKTPWVLGFNGKSLDDPAPALWAVKKDGGVFDQFAGATITPR
EEEECCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHCCCEECHH
AVVGAVKRGLELYTGNREAMIAAGAPRS
HHHHHHHCCEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]