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The map label for this gene is lipB

Identifier: 119896475

GI number: 119896475

Start: 199264

End: 199947

Strand: Direct

Name: lipB

Synonym: azo0183

Alternate gene names: 119896475

Gene position: 199264-199947 (Clockwise)

Preceding gene: 119896474

Following gene: 119896476

Centisome position: 4.55

GC content: 67.69

Gene sequence:

>684_bases
GTGACGGCTGCCGCCGTCCTCGCCGGGCACGAGGGGCCGGCCGCCGCCGTTTCCGTTCCGTTGCTGGTCAAGCGTCTCGG
CCGGGTGGACTACGCCCCGGCATGGGAGGCAATGCAGCACTTCACGGCCAGCCGTGGCGAGGACACCGCCGACGAGATCT
GGCTGCTGGAGCATCCGCCGGTCTATACGCTGGGCCAGGCCGGCCGGCCTGAACATCTGCTGCGCAACGACGCAGGCATT
CCCCTGGTGAAGATCGACCGCGGCGGCCAGATCACCTATCACGGTCCGGGCCAGCTCGTTGCCTATCTGCTGCTGGACTT
GCGCCGACGTCACCTGAAGGTGCGCGAGCTGGTGGCGTTGATGGAGCAGGCGGTCATCGACTGCCTTGCCGAGTATGGCT
TGCATGCCGAACGCAAGGACGGCGCCCCCGGCGTCTACATTGACGGCGCTAAGATCGCGGCGCTCGGCCTGCGGGTGCGC
AACGGCTGCAGCTACCACGGCCTGGCGCTCAACGTGGATGCCGACCTTGCCCCATTCGGCTGGATCAACCCCTGCGGCTA
TGAAGGCCTGCAGACGATACGGCTGAAGGATTTCGGCGTAGGCGACGACGTTGCGGCGGTGGGCGAGCGCCTGTTGCAAC
ACCTGCTGCGGCTTTTGCCGCCCGGCGTGGTACCGTCGCGCTGA

Upstream 100 bases:

>100_bases
ACTACCTCGCCGTCACCTGCACCTTCCGCGCCATCTCGCAGCAGCAGGTCGACGCGCTGTACATGGAGCTGACCTCGCAT
CCGATGGTGAAGGTGGTGCT

Downstream 100 bases:

>100_bases
GCGGTCACCCGCCACCCATACCAAAGAGAGACAGAGCATCCATGGATAACCCCGCACGCAAGCAGCGCGGCGCCGAGAAG
ACCGCGCGCATCCCGATCAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTAAAVLAGHEGPAAAVSVPLLVKRLGRVDYAPAWEAMQHFTASRGEDTADEIWLLEHPPVYTLGQAGRPEHLLRNDAGI
PLVKIDRGGQITYHGPGQLVAYLLLDLRRRHLKVRELVALMEQAVIDCLAEYGLHAERKDGAPGVYIDGAKIAALGLRVR
NGCSYHGLALNVDADLAPFGWINPCGYEGLQTIRLKDFGVGDDVAAVGERLLQHLLRLLPPGVVPSR

Sequences:

>Translated_227_residues
MTAAAVLAGHEGPAAAVSVPLLVKRLGRVDYAPAWEAMQHFTASRGEDTADEIWLLEHPPVYTLGQAGRPEHLLRNDAGI
PLVKIDRGGQITYHGPGQLVAYLLLDLRRRHLKVRELVALMEQAVIDCLAEYGLHAERKDGAPGVYIDGAKIAALGLRVR
NGCSYHGLALNVDADLAPFGWINPCGYEGLQTIRLKDFGVGDDVAAVGERLLQHLLRLLPPGVVPSR
>Mature_226_residues
TAAAVLAGHEGPAAAVSVPLLVKRLGRVDYAPAWEAMQHFTASRGEDTADEIWLLEHPPVYTLGQAGRPEHLLRNDAGIP
LVKIDRGGQITYHGPGQLVAYLLLDLRRRHLKVRELVALMEQAVIDCLAEYGLHAERKDGAPGVYIDGAKIAALGLRVRN
GCSYHGLALNVDADLAPFGWINPCGYEGLQTIRLKDFGVGDDVAAVGERLLQHLLRLLPPGVVPSR

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=217, Percent_Identity=34.5622119815668, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081767, Length=199, Percent_Identity=52.2613065326633, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323268, Length=171, Percent_Identity=29.2397660818713, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355483, Length=179, Percent_Identity=33.5195530726257, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_AZOSB (A1K1U6)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931688.1
- ProteinModelPortal:   A1K1U6
- SMR:   A1K1U6
- STRING:   A1K1U6
- GeneID:   4606250
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0183
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- PhylomeDB:   A1K1U6
- ProtClustDB:   PRK14343
- BioCyc:   ASP62928:AZO0183-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24567; Mature: 24435

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAAVLAGHEGPAAAVSVPLLVKRLGRVDYAPAWEAMQHFTASRGEDTADEIWLLEHPP
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCEEEECCCC
VYTLGQAGRPEHLLRNDAGIPLVKIDRGGQITYHGPGQLVAYLLLDLRRRHLKVRELVAL
EEECCCCCCCHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
MEQAVIDCLAEYGLHAERKDGAPGVYIDGAKIAALGLRVRNGCSYHGLALNVDADLAPFG
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHCCCCCCCCEEEECCCCCCCCC
WINPCGYEGLQTIRLKDFGVGDDVAAVGERLLQHLLRLLPPGVVPSR
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAAAVLAGHEGPAAAVSVPLLVKRLGRVDYAPAWEAMQHFTASRGEDTADEIWLLEHPP
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCEEEECCCC
VYTLGQAGRPEHLLRNDAGIPLVKIDRGGQITYHGPGQLVAYLLLDLRRRHLKVRELVAL
EEECCCCCCCHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
MEQAVIDCLAEYGLHAERKDGAPGVYIDGAKIAALGLRVRNGCSYHGLALNVDADLAPFG
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHCCCCCCCCEEEECCCCCCCCC
WINPCGYEGLQTIRLKDFGVGDDVAAVGERLLQHLLRLLPPGVVPSR
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA