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The map label for this gene is lipA [H]

Identifier: 119896476

GI number: 119896476

Start: 199989

End: 200936

Strand: Direct

Name: lipA [H]

Synonym: azo0184

Alternate gene names: 119896476

Gene position: 199989-200936 (Clockwise)

Preceding gene: 119896475

Following gene: 119896477

Centisome position: 4.57

GC content: 64.98

Gene sequence:

>948_bases
ATGGATAACCCCGCACGCAAGCAGCGCGGCGCCGAGAAGACCGCGCGCATCCCGATCAAGATCGTTCCTGCCGAACGGCT
GAAGAAGCCGGACTGGATCCGGATCCGCCTCGGTGCGGGCGCCGAGGCCGAGCGTTTCAACGAAATCAAGCAGACCCTGC
GCGAGCACAAGCTGCATACGGTGTGCGAAGAAGCCTCCTGCCCCAACATCCACGAGTGTTTCGGCAAGGGAACGGCCACC
TTCATGATCATGGGCGACATCTGCACCCGGCGCTGCCCGTTCTGCGATGTCGGGCATGGCCGTCCGGAGCCGCTCAATCC
CAACGAACCGACGGATCTAGCCCGCACGATCGCGGCGATGCGGCTCAACTATGTCGTGATCACCTCGGTCGACCGGGATG
ATCTGCGCGACGGCGGTGCCCAGCACTTCGTCGACTGCATCCGCGAAACCCGCGCCGCCTCGCCGTCCACCACGATCGAG
GTGCTGGTGCCCGATTTCCGCGGCCGCATGGAGATCGCGCTGGAGATCTTCAACCAGGCGCCGCCCGACGTGATGAACCA
CAACATGGAAACGGTGCCGCGGCTGTACAAACAGGCGCGCCCGGGGGCCGACTATGCCTATTCCCTGCGCCTGCTGAAGG
AATTCAAGGCGGGTCATCCCGACGTGCTCACCAAGTCCGGCCTCATGGTGGGGCTGGGGGAAACCGATGACGAAATCCTC
GACGTGATGCGCGACCTGCGCGCCCACGACGTGGACATGCTGACCATCGGCCAGTACCTGCAACCGTCCGGCGGTCACCT
GCCGGTGCTGCGCTACGTCCATCCCGATACCTTCAAGATGTTCGAAACCGAGGCGCTGCGGATGGGTTTCCGCAACGCGG
CCTGCGGTCCGATGGTGCGCTCGTCCTACTGGGCGGATCAGCAGGCCCACGGCGCGGGCGTGGTGTAG

Upstream 100 bases:

>100_bases
AGCGCCTGTTGCAACACCTGCTGCGGCTTTTGCCGCCCGGCGTGGTACCGTCGCGCTGAGCGGTCACCCGCCACCCATAC
CAAAGAGAGACAGAGCATCC

Downstream 100 bases:

>100_bases
CCGCAGCGCGCCGTGCCCCCGTTGCCGCCGTACGACGGGTGCGGCGGCGTTCTGCTCCCGGAGCCATCATGAAATTCCTG
CAGAAAACCCTGATCCTGTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MDNPARKQRGAEKTARIPIKIVPAERLKKPDWIRIRLGAGAEAERFNEIKQTLREHKLHTVCEEASCPNIHECFGKGTAT
FMIMGDICTRRCPFCDVGHGRPEPLNPNEPTDLARTIAAMRLNYVVITSVDRDDLRDGGAQHFVDCIRETRAASPSTTIE
VLVPDFRGRMEIALEIFNQAPPDVMNHNMETVPRLYKQARPGADYAYSLRLLKEFKAGHPDVLTKSGLMVGLGETDDEIL
DVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALRMGFRNAACGPMVRSSYWADQQAHGAGVV

Sequences:

>Translated_315_residues
MDNPARKQRGAEKTARIPIKIVPAERLKKPDWIRIRLGAGAEAERFNEIKQTLREHKLHTVCEEASCPNIHECFGKGTAT
FMIMGDICTRRCPFCDVGHGRPEPLNPNEPTDLARTIAAMRLNYVVITSVDRDDLRDGGAQHFVDCIRETRAASPSTTIE
VLVPDFRGRMEIALEIFNQAPPDVMNHNMETVPRLYKQARPGADYAYSLRLLKEFKAGHPDVLTKSGLMVGLGETDDEIL
DVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALRMGFRNAACGPMVRSSYWADQQAHGAGVV
>Mature_315_residues
MDNPARKQRGAEKTARIPIKIVPAERLKKPDWIRIRLGAGAEAERFNEIKQTLREHKLHTVCEEASCPNIHECFGKGTAT
FMIMGDICTRRCPFCDVGHGRPEPLNPNEPTDLARTIAAMRLNYVVITSVDRDDLRDGGAQHFVDCIRETRAASPSTTIE
VLVPDFRGRMEIALEIFNQAPPDVMNHNMETVPRLYKQARPGADYAYSLRLLKEFKAGHPDVLTKSGLMVGLGETDDEIL
DVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALRMGFRNAACGPMVRSSYWADQQAHGAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=46.2837837837838, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI37577164, Length=258, Percent_Identity=46.8992248062016, Blast_Score=231, Evalue=7e-61,
Organism=Escherichia coli, GI1786846, Length=311, Percent_Identity=64.3086816720257, Blast_Score=409, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=43.5087719298246, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=290, Percent_Identity=43.1034482758621, Blast_Score=238, Evalue=9e-64,
Organism=Drosophila melanogaster, GI221513272, Length=313, Percent_Identity=45.0479233226837, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35336; Mature: 35336

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNPARKQRGAEKTARIPIKIVPAERLKKPDWIRIRLGAGAEAERFNEIKQTLREHKLHT
CCCHHHHHCCCCHHHCCCEEEECHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VCEEASCPNIHECFGKGTATFMIMGDICTRRCPFCDVGHGRPEPLNPNEPTDLARTIAAM
HHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLNYVVITSVDRDDLRDGGAQHFVDCIRETRAASPSTTIEVLVPDFRGRMEIALEIFNQA
HHCEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCC
PPDVMNHNMETVPRLYKQARPGADYAYSLRLLKEFKAGHPDVLTKSGLMVGLGETDDEIL
CHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEECCCCHHHHH
DVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALRMGFRNAACGPMVR
HHHHHHHHCCCCCEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHH
SSYWADQQAHGAGVV
HHHCCCCCCCCCCCC
>Mature Secondary Structure
MDNPARKQRGAEKTARIPIKIVPAERLKKPDWIRIRLGAGAEAERFNEIKQTLREHKLHT
CCCHHHHHCCCCHHHCCCEEEECHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VCEEASCPNIHECFGKGTATFMIMGDICTRRCPFCDVGHGRPEPLNPNEPTDLARTIAAM
HHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLNYVVITSVDRDDLRDGGAQHFVDCIRETRAASPSTTIEVLVPDFRGRMEIALEIFNQA
HHCEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCC
PPDVMNHNMETVPRLYKQARPGADYAYSLRLLKEFKAGHPDVLTKSGLMVGLGETDDEIL
CHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEECCCCHHHHH
DVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALRMGFRNAACGPMVR
HHHHHHHHCCCCCEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHH
SSYWADQQAHGAGVV
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA