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The map label for this gene is gyrB [H]

Identifier: 119896295

GI number: 119896295

Start: 2821

End: 5310

Strand: Direct

Name: gyrB [H]

Synonym: azo0003

Alternate gene names: 119896295

Gene position: 2821-5310 (Clockwise)

Preceding gene: 119896294

Following gene: 119896296

Centisome position: 0.06

GC content: 65.26

Gene sequence:

>2490_bases
ATGTCCGAACCGCAAAACACGCCCGCCCCCTCCGGCAACGACTACGACGAATCCAGCATCCAGCAGCTCGAAGGCCTGGA
AGCGGTGAGGAAGCGGCCGGGCATGTACATCGGCGACACCTCCGACGGCACCGGCCTGCACCACATGGTGTTCGAGGTCG
TCGACAACGCCATCGACGAGGCGCTGGCCGGCCACTGCGACGACATCGTCATCACCATCCACGCCGACAACTCCATCTCC
GTCACCGACAACGGCCGCGGCATCCCGGTCGGCGTCAAGATGGACGACAAGCACGAGCCCAAGCGCTCCGCGGCCGAAAT
CGTCATGTGCGTGCTGCACGCCGGCGGCAAGTTCAACCAGAACAGCTACAAGGTTTCGGGCGGCCTGCACGGCGTCGGCG
TGTCCTGCGTCAACGCGCTGTCGAAGTGGCTGCGCCTCACCATCCGCCGCGACGGCAAGAAGCACTTCATGGAGTTCCAC
CGCGGCGCGCCGCAGGACCGCGTGATCGACGTGGTGGACGGCGTCGAAGTGAGCCCGCTGAAGGTGCTGGGCGAAACCAG
CAAGCGCGGTACCGAGGTGCATTACCTCGCCGACGAGGAAATCTTCGGCACCGTCGAGTACCACTACGAAATCCTCGCCA
AGCGTCTGCGCGAACTGTCGTTCCTCAACAACGGCGTCAAGATCCGCCTGGTCGACCAGCGCACCGGCAAGGAAGAAGAC
TTCGCCTTCGCCGGCGGCGTGCGCGGCTTCGTCGAGTACATCAACCGCACCAAGGCGGTCCTGCATCCCAGCGTGTTCTA
CGCCGCCGGCACCACCCGCATCCCCACTGGCGCCGGCGAGGCCGAACTTTCGGTCGAAGTCGCCATGCAATGGAACGACT
CCTACGCCGAACAGGTGCTCTGCTACACCAACAACATCCCGCAGGCCGACGGCGGCACCCACCTCACCGGCCTGCGCACC
GCGATGACCCGCGTCATCAACAAGTACATCGAAGAAAACGAGATCGCCAAGAAGGCCAAGGTGGACATCACCGGCGACGA
CATGCGCGAAGGCCTCGCCTGCGTGCTGTCGGTGAAGATGCCGGACCCCAAGTTCGCCAGCCAGACCAAGATGAAACTCG
TCTCCAGCGAGGCGCGCCCGGCGGTGGAAGAAGTCGTCGCCGCCAAGCTCACTGACTTCCTGCTCGAAAACCCGATCGAC
GCCAAGACCATCTGCAACAAGATCGTCGAAGCCGCCCGCGCCCGCGACGCCGCCCGCAAGGCGCGCGAAATGACCCGCCG
CAAGGGCGTGCTCGACGGCGTCGGCCTGCCCGGCAAGCTCGCCGACTGCCAGGAAAAAGACCCCGCGCTGTCCGAGCTTT
ACCTGGTCGAGGGTGACTCCGCAGGCGGCTCCGCCAAGCAGGGCCGCGACCGCAAGTTCCAGGCGATCCTGCCGCTCAAG
GGCAAAATCCTCAACGTCGAGAAGGCCCGCTTCGACAAGCTGCTGCAGAGCCAGGAAATCGCCACCCTCATCACCGCGCT
CGGCACCAGCATCGGCAAGGACGACTACAAGCCCGAAAAGCTGCGCTACCACCGCATCATCCTGATGACCGACGCCGACG
TCGACGGCGCCCACATCCGCACCCTGCTGCTCACCTTCTTCTACCGCCAGATGCCGGAGCTGGTCGAGCGCGGCCATATC
TACATCGCCCAGCCGCCGCTCTACAAGATCAAGCACGGCAAGACCGAGCTCTACATCAAGGACGACCACGAACTCAACGG
CCACCTGCTCAAGCTCGCGCTCGACGGCGCCGTGCTGGTGCCGCGCGAAGGCGCCGACGCCATCACCGACGACGCACTCG
GCGGCCTCGCGCGCAGCTACCTGCTCGCAGAAGCGGTCATCAAGCGCCTGGCCAGCTACATCGACCCCGAAGTGCTGCAA
GCCATGCTCGCGCACGACCTCGAGATCAGCCTGGACGACGAAGCCGCAGCCCGCGCATCGGCCGACCGCATCCAGCCGCA
CCTGCCGGAAGGCCTGCGCATCTACGCCGAATACCACGAAGAAACCGAAGCCTGGCGCCTCACCATCGAGCGCCTGCACC
ACGGCAACCTCAAGTTCGGCTGGGTCGAAACCGCCTTCCTTGTCTCCGGCGACTACCGCAGCATCCGCACCGCCGCCGCC
TCCATCGCCGGCCTCATCGGCCCCGGCGCCGAAATCCGCCGCGGCGAAAAGCGCCAGACCGTCACCCGCTTCGCCGACGC
CATCACCTGGCTGCTGGCCGAAGTGGAACGCGGCCTCAGCAAGCAGCGCTACAAGGGCCTCGGCGAGATGAACCCCGAGC
AGTTGTGGGAAACCACCATGGACCCGGCAGTCCGCCGCCTGCTGCGGGTGCAGATCGACGACGCGATTGCGGCGGATGAA
ATCTTCACCACGCTGATGGGGGATAACGTGGAGCCGAGAAGGGCATTCATCGAGAGTAATGCGCTGTATGCGCAGAACAT
CGACGTGTGA

Upstream 100 bases:

>100_bases
CTCGCCGCCACGCAGGTGTCGAGCAGTGCTCGCCGAAACCCCAGCTCCGCCCCCTTGAACGGGAAGGAGTTCCGTTCTTT
TTGAATCACCCGAGATGACA

Downstream 100 bases:

>100_bases
CGGATTGGCGCAAGTAAATGCCTGAGCCAACGCATACGCCCAGACAAAAATAGATCACCGTTTCTCGCACCAGCGCGACA
AACGTTTAAAGCATCGAATA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 828

Protein sequence:

>829_residues
MSEPQNTPAPSGNDYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGHCDDIVITIHADNSIS
VTDNGRGIPVGVKMDDKHEPKRSAAEIVMCVLHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKKHFMEFH
RGAPQDRVIDVVDGVEVSPLKVLGETSKRGTEVHYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGKEED
FAFAGGVRGFVEYINRTKAVLHPSVFYAAGTTRIPTGAGEAELSVEVAMQWNDSYAEQVLCYTNNIPQADGGTHLTGLRT
AMTRVINKYIEENEIAKKAKVDITGDDMREGLACVLSVKMPDPKFASQTKMKLVSSEARPAVEEVVAAKLTDFLLENPID
AKTICNKIVEAARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLK
GKILNVEKARFDKLLQSQEIATLITALGTSIGKDDYKPEKLRYHRIILMTDADVDGAHIRTLLLTFFYRQMPELVERGHI
YIAQPPLYKIKHGKTELYIKDDHELNGHLLKLALDGAVLVPREGADAITDDALGGLARSYLLAEAVIKRLASYIDPEVLQ
AMLAHDLEISLDDEAAARASADRIQPHLPEGLRIYAEYHEETEAWRLTIERLHHGNLKFGWVETAFLVSGDYRSIRTAAA
SIAGLIGPGAEIRRGEKRQTVTRFADAITWLLAEVERGLSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIDDAIAADE
IFTTLMGDNVEPRRAFIESNALYAQNIDV

Sequences:

>Translated_829_residues
MSEPQNTPAPSGNDYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGHCDDIVITIHADNSIS
VTDNGRGIPVGVKMDDKHEPKRSAAEIVMCVLHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKKHFMEFH
RGAPQDRVIDVVDGVEVSPLKVLGETSKRGTEVHYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGKEED
FAFAGGVRGFVEYINRTKAVLHPSVFYAAGTTRIPTGAGEAELSVEVAMQWNDSYAEQVLCYTNNIPQADGGTHLTGLRT
AMTRVINKYIEENEIAKKAKVDITGDDMREGLACVLSVKMPDPKFASQTKMKLVSSEARPAVEEVVAAKLTDFLLENPID
AKTICNKIVEAARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLK
GKILNVEKARFDKLLQSQEIATLITALGTSIGKDDYKPEKLRYHRIILMTDADVDGAHIRTLLLTFFYRQMPELVERGHI
YIAQPPLYKIKHGKTELYIKDDHELNGHLLKLALDGAVLVPREGADAITDDALGGLARSYLLAEAVIKRLASYIDPEVLQ
AMLAHDLEISLDDEAAARASADRIQPHLPEGLRIYAEYHEETEAWRLTIERLHHGNLKFGWVETAFLVSGDYRSIRTAAA
SIAGLIGPGAEIRRGEKRQTVTRFADAITWLLAEVERGLSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIDDAIAADE
IFTTLMGDNVEPRRAFIESNALYAQNIDV
>Mature_828_residues
SEPQNTPAPSGNDYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGHCDDIVITIHADNSISV
TDNGRGIPVGVKMDDKHEPKRSAAEIVMCVLHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKKHFMEFHR
GAPQDRVIDVVDGVEVSPLKVLGETSKRGTEVHYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGKEEDF
AFAGGVRGFVEYINRTKAVLHPSVFYAAGTTRIPTGAGEAELSVEVAMQWNDSYAEQVLCYTNNIPQADGGTHLTGLRTA
MTRVINKYIEENEIAKKAKVDITGDDMREGLACVLSVKMPDPKFASQTKMKLVSSEARPAVEEVVAAKLTDFLLENPIDA
KTICNKIVEAARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVEKARFDKLLQSQEIATLITALGTSIGKDDYKPEKLRYHRIILMTDADVDGAHIRTLLLTFFYRQMPELVERGHIY
IAQPPLYKIKHGKTELYIKDDHELNGHLLKLALDGAVLVPREGADAITDDALGGLARSYLLAEAVIKRLASYIDPEVLQA
MLAHDLEISLDDEAAARASADRIQPHLPEGLRIYAEYHEETEAWRLTIERLHHGNLKFGWVETAFLVSGDYRSIRTAAAS
IAGLIGPGAEIRRGEKRQTVTRFADAITWLLAEVERGLSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIDDAIAADEI
FTTLMGDNVEPRRAFIESNALYAQNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=609, Percent_Identity=24.4663382594417, Blast_Score=106, Evalue=1e-22,
Organism=Homo sapiens, GI19913406, Length=599, Percent_Identity=23.5392320534224, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI48994957, Length=826, Percent_Identity=58.9588377723971, Blast_Score=926, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=581, Percent_Identity=39.2426850258176, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=604, Percent_Identity=24.0066225165563, Blast_Score=119, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=525, Percent_Identity=23.2380952380952, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=162, Percent_Identity=31.4814814814815, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=590, Percent_Identity=24.406779661017, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17136538, Length=581, Percent_Identity=25.6454388984509, Blast_Score=123, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91765; Mature: 91633

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPQNTPAPSGNDYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHCDDIVITIHADNSISVTDNGRGIPVGVKMDDKHEPKRSAAEIVMCVLHAGGKFNQ
HHHCCCCCEEEEEECCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
NSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKKHFMEFHRGAPQDRVIDVVDGVEVSPL
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEHHHCCCCCCHH
KVLGETSKRGTEVHYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGKEED
HHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
FAFAGGVRGFVEYINRTKAVLHPSVFYAAGTTRIPTGAGEAELSVEVAMQWNDSYAEQVL
CCHHCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCHHHHEEE
CYTNNIPQADGGTHLTGLRTAMTRVINKYIEENEIAKKAKVDITGDDMREGLACVLSVKM
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCEEEEEEEEC
PDPKFASQTKMKLVSSEARPAVEEVVAAKLTDFLLENPIDAKTICNKIVEAARARDAARK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AREMTRRKGVLDGVGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC
GKILNVEKARFDKLLQSQEIATLITALGTSIGKDDYKPEKLRYHRIILMTDADVDGAHIR
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHH
TLLLTFFYRQMPELVERGHIYIAQPPLYKIKHGKTELYIKDDHELNGHLLKLALDGAVLV
HHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCEEEEEECCCCCCCEEEEEEECCEEEE
PREGADAITDDALGGLARSYLLAEAVIKRLASYIDPEVLQAMLAHDLEISLDDEAAARAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCHHHHHHH
ADRIQPHLPEGLRIYAEYHEETEAWRLTIERLHHGNLKFGWVETAFLVSGDYRSIRTAAA
HHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHH
SIAGLIGPGAEIRRGEKRQTVTRFADAITWLLAEVERGLSKQRYKGLGEMNPEQLWETTM
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
DPAVRRLLRVQIDDAIAADEIFTTLMGDNVEPRRAFIESNALYAQNIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SEPQNTPAPSGNDYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHCDDIVITIHADNSISVTDNGRGIPVGVKMDDKHEPKRSAAEIVMCVLHAGGKFNQ
HHHCCCCCEEEEEECCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
NSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKKHFMEFHRGAPQDRVIDVVDGVEVSPL
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEHHHCCCCCCHH
KVLGETSKRGTEVHYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGKEED
HHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
FAFAGGVRGFVEYINRTKAVLHPSVFYAAGTTRIPTGAGEAELSVEVAMQWNDSYAEQVL
CCHHCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCHHHHEEE
CYTNNIPQADGGTHLTGLRTAMTRVINKYIEENEIAKKAKVDITGDDMREGLACVLSVKM
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCEEEEEEEEC
PDPKFASQTKMKLVSSEARPAVEEVVAAKLTDFLLENPIDAKTICNKIVEAARARDAARK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AREMTRRKGVLDGVGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC
GKILNVEKARFDKLLQSQEIATLITALGTSIGKDDYKPEKLRYHRIILMTDADVDGAHIR
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHH
TLLLTFFYRQMPELVERGHIYIAQPPLYKIKHGKTELYIKDDHELNGHLLKLALDGAVLV
HHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCEEEEEECCCCCCCEEEEEEECCEEEE
PREGADAITDDALGGLARSYLLAEAVIKRLASYIDPEVLQAMLAHDLEISLDDEAAARAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCHHHHHHH
ADRIQPHLPEGLRIYAEYHEETEAWRLTIERLHHGNLKFGWVETAFLVSGDYRSIRTAAA
HHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHH
SIAGLIGPGAEIRRGEKRQTVTRFADAITWLLAEVERGLSKQRYKGLGEMNPEQLWETTM
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
DPAVRRLLRVQIDDAIAADEIFTTLMGDNVEPRRAFIESNALYAQNIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]