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The map label for this gene is dnaN [H]

Identifier: 119896294

GI number: 119896294

Start: 1502

End: 2611

Strand: Direct

Name: dnaN [H]

Synonym: azo0002

Alternate gene names: 119896294

Gene position: 1502-2611 (Clockwise)

Preceding gene: 119896293

Following gene: 119896295

Centisome position: 0.03

GC content: 65.59

Gene sequence:

>1110_bases
ATGCTTCTGCTCAACACCACCCGCGACGCGCTCCTCGCCCCGCTGCAGTCGGTAGCCGGCATTGTCGAAAAGCGCCACAC
CCTGCCGATTCTCTCCAACGTGCTGATCGAGAAGCGCGGCGACCAGCTCACCTTGCTCGCCACCGACATCGAGATCCAGA
TCCGCACCACCACCGCCGGCCACATCGGCGGCGAGGATTCGTCGATCACGGTCGGCGCGCGCAAGCTGCAGGACATCCTG
CGCGCACTGTCGGACAGCGTTGATATCGTGCTGACGCTCGAAGACAAGCGCCTCACCGTGAAGGCTGGCAAGAGCCGCTT
CCAGCTGCAGACCCTGCCTGCCGCCGACTATCCGCGCATGAACCTGCCCGACGGCGACGCGGTGCGCTTCTCGGTGCCGC
AGCGCGCCTTCAAGCGCCAGCTCGCGCAGGTCTCGTACTCGATGGCGCAGCAGGACATCCGCTACTACCTCAACGGCCTG
CTGCTGATCGCCGACGGCTCCGAACTGCGCATGGTAGCCACCGACGGCCACCGCCTCGCCTACGCCGCGGGCGACCTCGC
GGTGCCGGTGCAGGCGCGCACCGAAGCCATCCTGCCGCGCAAGACGGTGCTCGAGCTCGCCCGCCAGCTGGCCGACACCG
ACGATCCGCTGGAAATCATCCTCGCCGGCAACCAGGCGGTGTTCCGCTTCGGCTCGATCGAACTCGTCACCAAGCTGATC
GACGGCAAGTTCCCCGACTACGAGCGCGTCATCCCGCAGAACCATCCGCGCATGGTCACCTTCGACCGCCAGCCGCTGCT
CGCCTCGCTGCAACGCGCCGCCATCCTCACCAACGAGAAATTCCGCGGCGTGCGCATGGTGCTCGGCGAGGGCGCGCTCA
AGATCATCAGCTCCAACACCGAGCAGGAAGAAGCGCAGGAAGAGCTGGAGATCGACTACCACGACACCCCGCTCGACATC
GGCTTCAACGTCACCTACCTGCTCGACGTGCTCAACAACCTGTCGTCCGAGCAGGTGGAATGGCGCTTCAACGACGGCAA
CTCCAGCGCGCTCGTCACGCTGCCGGGCAACGACAAGTTCAAGTACGTCGTGATGCCGATGCGCATCTAG

Upstream 100 bases:

>100_bases
AACCACGACGTCCATGTGCTCACCCAGGTGCTGCGGGGTTAAGCGCGAATGACGACGCGGATTTGCCAGACACCGACCAA
CCACTCCCTCAGCTGACGCC

Downstream 100 bases:

>100_bases
CCCCGCTCCTCCCCCTTCAAGGGGGAGGCCAGGAGGGGGATGGGGTCAGCCGCTGCGATCCACCGCCCCATCCCCACCTC
GAAAAATGCAGTTGTAGTCT

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLLLNTTRDALLAPLQSVAGIVEKRHTLPILSNVLIEKRGDQLTLLATDIEIQIRTTTAGHIGGEDSSITVGARKLQDIL
RALSDSVDIVLTLEDKRLTVKAGKSRFQLQTLPAADYPRMNLPDGDAVRFSVPQRAFKRQLAQVSYSMAQQDIRYYLNGL
LLIADGSELRMVATDGHRLAYAAGDLAVPVQARTEAILPRKTVLELARQLADTDDPLEIILAGNQAVFRFGSIELVTKLI
DGKFPDYERVIPQNHPRMVTFDRQPLLASLQRAAILTNEKFRGVRMVLGEGALKIISSNTEQEEAQEELEIDYHDTPLDI
GFNVTYLLDVLNNLSSEQVEWRFNDGNSSALVTLPGNDKFKYVVMPMRI

Sequences:

>Translated_369_residues
MLLLNTTRDALLAPLQSVAGIVEKRHTLPILSNVLIEKRGDQLTLLATDIEIQIRTTTAGHIGGEDSSITVGARKLQDIL
RALSDSVDIVLTLEDKRLTVKAGKSRFQLQTLPAADYPRMNLPDGDAVRFSVPQRAFKRQLAQVSYSMAQQDIRYYLNGL
LLIADGSELRMVATDGHRLAYAAGDLAVPVQARTEAILPRKTVLELARQLADTDDPLEIILAGNQAVFRFGSIELVTKLI
DGKFPDYERVIPQNHPRMVTFDRQPLLASLQRAAILTNEKFRGVRMVLGEGALKIISSNTEQEEAQEELEIDYHDTPLDI
GFNVTYLLDVLNNLSSEQVEWRFNDGNSSALVTLPGNDKFKYVVMPMRI
>Mature_369_residues
MLLLNTTRDALLAPLQSVAGIVEKRHTLPILSNVLIEKRGDQLTLLATDIEIQIRTTTAGHIGGEDSSITVGARKLQDIL
RALSDSVDIVLTLEDKRLTVKAGKSRFQLQTLPAADYPRMNLPDGDAVRFSVPQRAFKRQLAQVSYSMAQQDIRYYLNGL
LLIADGSELRMVATDGHRLAYAAGDLAVPVQARTEAILPRKTVLELARQLADTDDPLEIILAGNQAVFRFGSIELVTKLI
DGKFPDYERVIPQNHPRMVTFDRQPLLASLQRAAILTNEKFRGVRMVLGEGALKIISSNTEQEEAQEELEIDYHDTPLDI
GFNVTYLLDVLNNLSSEQVEWRFNDGNSSALVTLPGNDKFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=363, Percent_Identity=44.0771349862259, Blast_Score=295, Evalue=4e-81,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41089; Mature: 41089

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLNTTRDALLAPLQSVAGIVEKRHTLPILSNVLIEKRGDQLTLLATDIEIQIRTTTAG
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECC
HIGGEDSSITVGARKLQDILRALSDSVDIVLTLEDKRLTVKAGKSRFQLQTLPAADYPRM
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEEECCCCCCCCC
NLPDGDAVRFSVPQRAFKRQLAQVSYSMAQQDIRYYLNGLLLIADGSELRMVATDGHRLA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCEEE
YAAGDLAVPVQARTEAILPRKTVLELARQLADTDDPLEIILAGNQAVFRFGSIELVTKLI
EECCCEEEECHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHH
DGKFPDYERVIPQNHPRMVTFDRQPLLASLQRAAILTNEKFRGVRMVLGEGALKIISSNT
CCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCC
EQEEAQEELEIDYHDTPLDIGFNVTYLLDVLNNLSSEQVEWRFNDGNSSALVTLPGNDKF
CHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCE
KYVVMPMRI
EEEEEEECC
>Mature Secondary Structure
MLLLNTTRDALLAPLQSVAGIVEKRHTLPILSNVLIEKRGDQLTLLATDIEIQIRTTTAG
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECC
HIGGEDSSITVGARKLQDILRALSDSVDIVLTLEDKRLTVKAGKSRFQLQTLPAADYPRM
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEEECCCCCCCCC
NLPDGDAVRFSVPQRAFKRQLAQVSYSMAQQDIRYYLNGLLLIADGSELRMVATDGHRLA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCEEE
YAAGDLAVPVQARTEAILPRKTVLELARQLADTDDPLEIILAGNQAVFRFGSIELVTKLI
EECCCEEEECHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHH
DGKFPDYERVIPQNHPRMVTFDRQPLLASLQRAAILTNEKFRGVRMVLGEGALKIISSNT
CCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCC
EQEEAQEELEIDYHDTPLDIGFNVTYLLDVLNNLSSEQVEWRFNDGNSSALVTLPGNDKF
CHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCE
KYVVMPMRI
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]