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The map label for this gene is gluQ [H]

Identifier: 119871056

GI number: 119871056

Start: 5269206

End: 5270087

Strand: Direct

Name: gluQ [H]

Synonym: Mkms_5029

Alternate gene names: 119871056

Gene position: 5269206-5270087 (Clockwise)

Preceding gene: 119871054

Following gene: 119871057

Centisome position: 91.84

GC content: 71.88

Gene sequence:

>882_bases
GTGACCCGCTCCCCCGCGGGCCGGTTCGCGCCGAGCCCGTCGGCCGATCTGCACATCGGCAACCTGCGCACCGCCGTGCT
CGCCTGGCTCTTCGCCCGGTCGACCGGTCGGCGGTTCCTCATGCGCGTCGAGGACCTCGACTACCGCACCGATTCCGACA
TCGCCGCCCGCCAACTCGTCGACCTGGCCGCACTGGGCCTGACCTGGGACGGACCGGCCGAATACCAGTCCGCGCACCGC
GACCGCTACGACGGCGTCATCGACGGTCTGGTCGACGCCGGCATGGTGTACGAATGCTATTGCAGCAGAAAGGACATCGC
GCAGGCGCCGCGTGCGCCGCACGCACCGCAGGGGGCCTACCCCGGCATCTGCCGCGATCTCACCGACGCCGAACGCGACG
CGCGCCGCCGTGAGACGGGCCGTCCGCCCGCACTGCGGTTGCGCACCGACGTGATCACCTACACCGTCACCGACCTGCTG
CACGGCGCCCACACCGGCATCGTCGACGACTTCGTGGTCCGCCGCGGGGACGGTGTGCCCGCCTACAACCTGGCCGTGGT
GGTCGACGATGCGGCTGCGGGTGTCGACCAGGTGGTTCGCGGCGACGACCTGCTGTCGTCGTCGCCGCGGCAGGCGTATC
TGGCGCGGCTGCTCGGGTATCCAGAGCCGACGTATGCCCACGTGCCGCTGGTGCTCAACACCGACGGCGCCCGGCTGGCC
AAACGCGACGGTGCCGTGACCCTCGCCGAGATCGGGGCGCACACGGCGCTGCGCCGGATCGCCGATTCGCTGGGCTTCGC
CGGGGCCGACGTCGACGCGATGCTGGCGGAGTTCGACCCGGCCCGGCTGCCGCGGCACCCGTGGATATACGTGCCGGACT
GA

Upstream 100 bases:

>100_bases
GCGTCCCCCAGATGTATGTGTCCACGGCGGTCACACGTAAGCGTACGGGGTGGGACCGCCAGAATCCGAACCGACGCGCT
CAGGCGGCTAGATTGCGGTC

Downstream 100 bases:

>100_bases
TCGGAACCCTTCTGTGGCGGCGGCGGGCTTGTTAGCGTCCGCCGGTGAGGTTTCTTCGGGCGCTCCTGCTGCTGCTCGCC
GTCCTGACCGTGGCGTCCTG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTRSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLMRVEDLDYRTDSDIAARQLVDLAALGLTWDGPAEYQSAHR
DRYDGVIDGLVDAGMVYECYCSRKDIAQAPRAPHAPQGAYPGICRDLTDAERDARRRETGRPPALRLRTDVITYTVTDLL
HGAHTGIVDDFVVRRGDGVPAYNLAVVVDDAAAGVDQVVRGDDLLSSSPRQAYLARLLGYPEPTYAHVPLVLNTDGARLA
KRDGAVTLAEIGAHTALRRIADSLGFAGADVDAMLAEFDPARLPRHPWIYVPD

Sequences:

>Translated_293_residues
MTRSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLMRVEDLDYRTDSDIAARQLVDLAALGLTWDGPAEYQSAHR
DRYDGVIDGLVDAGMVYECYCSRKDIAQAPRAPHAPQGAYPGICRDLTDAERDARRRETGRPPALRLRTDVITYTVTDLL
HGAHTGIVDDFVVRRGDGVPAYNLAVVVDDAAAGVDQVVRGDDLLSSSPRQAYLARLLGYPEPTYAHVPLVLNTDGARLA
KRDGAVTLAEIGAHTALRRIADSLGFAGADVDAMLAEFDPARLPRHPWIYVPD
>Mature_292_residues
TRSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLMRVEDLDYRTDSDIAARQLVDLAALGLTWDGPAEYQSAHRD
RYDGVIDGLVDAGMVYECYCSRKDIAQAPRAPHAPQGAYPGICRDLTDAERDARRRETGRPPALRLRTDVITYTVTDLLH
GAHTGIVDDFVVRRGDGVPAYNLAVVVDDAAAGVDQVVRGDDLLSSSPRQAYLARLLGYPEPTYAHVPLVLNTDGARLAK
RDGAVTLAEIGAHTALRRIADSLGFAGADVDAMLAEFDPARLPRHPWIYVPD

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=267, Percent_Identity=33.7078651685393, Blast_Score=141, Evalue=6e-34,
Organism=Escherichia coli, GI1788743, Length=251, Percent_Identity=36.2549800796813, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI226510925, Length=278, Percent_Identity=35.9712230215827, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=32.6693227091633, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=264, Percent_Identity=31.0606060606061, Blast_Score=138, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24665321, Length=256, Percent_Identity=34.375, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 31955; Mature: 31824

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLMRVEDLDYRTDSDIAARQLV
CCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCHHHHHHHH
DLAALGLTWDGPAEYQSAHRDRYDGVIDGLVDAGMVYECYCSRKDIAQAPRAPHAPQGAY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHCCCCCCCCCCCC
PGICRDLTDAERDARRRETGRPPALRLRTDVITYTVTDLLHGAHTGIVDDFVVRRGDGVP
CHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
AYNLAVVVDDAAAGVDQVVRGDDLLSSSPRQAYLARLLGYPEPTYAHVPLVLNTDGARLA
EEEEEEEEECHHHCHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
KRDGAVTLAEIGAHTALRRIADSLGFAGADVDAMLAEFDPARLPRHPWIYVPD
HCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCCCEEEECC
>Mature Secondary Structure 
TRSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLMRVEDLDYRTDSDIAARQLV
CCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCHHHHHHHH
DLAALGLTWDGPAEYQSAHRDRYDGVIDGLVDAGMVYECYCSRKDIAQAPRAPHAPQGAY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHCCCCCCCCCCCC
PGICRDLTDAERDARRRETGRPPALRLRTDVITYTVTDLLHGAHTGIVDDFVVRRGDGVP
CHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
AYNLAVVVDDAAAGVDQVVRGDDLLSSSPRQAYLARLLGYPEPTYAHVPLVLNTDGARLA
EEEEEEEEECHHHCHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
KRDGAVTLAEIGAHTALRRIADSLGFAGADVDAMLAEFDPARLPRHPWIYVPD
HCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA