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The map label for this gene is radA [H]

Identifier: 119870856

GI number: 119870856

Start: 5056904

End: 5058304

Strand: Direct

Name: radA [H]

Synonym: Mkms_4828

Alternate gene names: 119870856

Gene position: 5056904-5058304 (Clockwise)

Preceding gene: 119870855

Following gene: 119870857

Centisome position: 88.14

GC content: 71.45

Gene sequence:

>1401_bases
GTGGCCAAGGCACGTTCTCAATACCGCTGTTCGGAGTGCCGGCACATCACCGCCAAATGGGTGGGCCGCTGCCCGGACTG
TGGCACGTGGGGAACGGTCGAGGAAGTCGCGGTCCTGGCCGCCGTGGCCGGCGCACCCGCCCGCCACGCGGTGGCGCCCG
CCTCCCCCGCTGTTCCGATCAGCAGCATCGACCCGGGCCGTACCAGACACGACGAGACCGGGATCAGCGAACTCGACCGG
GTCCTCGGCGGTGGGCTGGTACCCGGTTCGGTGACGCTGCTCGCGGGTGATCCTGGCGTCGGCAAGTCGACCCTGCTCCT
CGAGGTCGCGCACCGCTGGGCCGTGTCGGGTCGGCGTGCGCTGTACCTGACCGGTGAGGAGTCGGCCGGCCAGGTGCGCC
TGCGGGCCGAGCGCACCGGCTGTACCCACGACGAGCTGTATCTCGCCGCGGAATCCGATCTGCCCACCGCGCTGGGCCAC
ATCGACGCCGTGCAGCCGAGCCTCGTGGTCGTCGACTCCGTGCAGACCATGTCCACCAGCGAGAACGACGGCGTCACCGG
CGGCGTGACCCAGGTGCGGGCCGTCACCACCGCGCTCACCCAGTACGCCAAGGGGGCGGCGGGGCCGGGCGGCCCCGGTG
GCATCGCCATGGTGCTGGTCGGCCACGTCACCAAGGACGGGGCGATCGCCGGGCCGCGTTCGCTGGAACATCTGGTCGAC
GTGGTGCTGCATTTCGAGGGTGACCGCAACTCGACGCTGCGGATGGTGCGCGGGGTCAAGAACCGGTTCGGCGCAGCCGA
CGAGGTCGGCTGTTTCCAGTTGCACGACAACGGCATCGAATGCGTGGCCGATCCGTCCGGGCTCTTCCGCGACCAGCGCG
ACACCCCGGTGGCCGGCACCGCGGTGACGGTGGCCCTCGACGGTAAACGTCCACTCATCGGCGAGGTGCAGGCGCTGGTC
GGGCCGCCCACCGGACCGCATCCCCGCCGTGCCGTCAGCGGGATCGACGCCGCCCGCGCCGCGATGATCGCCGCCGTCCT
CGACAAACACGCCAGGCTGCAGGTGGGACCCTGCGACATCTACCTGTCGACGGTCGGCGGTATGCGGCTGACCGACCCGT
CGTCGGACCTCGCGGTCGCGATCGCAATGGCCTCCGCGCGCAAGGAACGGGCGATGCCGGCGAACTCGGTGGTGATCGGC
GAGCTCGGCCTCGCGGGTGATCTGCGGCGGGTGACAGGTATGGACCGCCGCCTCGCCGAGGCCGCCCGACTGGGGTTCAC
CACGGCCGTCGTACCGCCCGGCACGACGGGCGTACCGCCCGGCCTTCGAGTGCTGCCCGCCGAGAACATCTCGGCGGCGT
TGCGGGTCCTGGACCGCATCGCAGACAATGGCGGCCGATGA

Upstream 100 bases:

>100_bases
CGGCGGCGGCGGCTCCGAGCACTCCGGCGGACACTGACCCGTAGCGCGACACGGGCACGGCCCGAGTCGATCTGTCGGCC
CCGACCGATACCGTCGTCCC

Downstream 100 bases:

>100_bases
GCGCTCGCGCGAAGAGCAGACAGCAGCGATGAGCGCTTGCGCGAAGAGCAGACAGCAGCGATGAGCGCTTGCGCGAAGAG
CAGACAGCAGCGATGAGCGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKARSQYRCSECRHITAKWVGRCPDCGTWGTVEEVAVLAAVAGAPARHAVAPASPAVPISSIDPGRTRHDETGISELDR
VLGGGLVPGSVTLLAGDPGVGKSTLLLEVAHRWAVSGRRALYLTGEESAGQVRLRAERTGCTHDELYLAAESDLPTALGH
IDAVQPSLVVVDSVQTMSTSENDGVTGGVTQVRAVTTALTQYAKGAAGPGGPGGIAMVLVGHVTKDGAIAGPRSLEHLVD
VVLHFEGDRNSTLRMVRGVKNRFGAADEVGCFQLHDNGIECVADPSGLFRDQRDTPVAGTAVTVALDGKRPLIGEVQALV
GPPTGPHPRRAVSGIDAARAAMIAAVLDKHARLQVGPCDIYLSTVGGMRLTDPSSDLAVAIAMASARKERAMPANSVVIG
ELGLAGDLRRVTGMDRRLAEAARLGFTTAVVPPGTTGVPPGLRVLPAENISAALRVLDRIADNGGR

Sequences:

>Translated_466_residues
MAKARSQYRCSECRHITAKWVGRCPDCGTWGTVEEVAVLAAVAGAPARHAVAPASPAVPISSIDPGRTRHDETGISELDR
VLGGGLVPGSVTLLAGDPGVGKSTLLLEVAHRWAVSGRRALYLTGEESAGQVRLRAERTGCTHDELYLAAESDLPTALGH
IDAVQPSLVVVDSVQTMSTSENDGVTGGVTQVRAVTTALTQYAKGAAGPGGPGGIAMVLVGHVTKDGAIAGPRSLEHLVD
VVLHFEGDRNSTLRMVRGVKNRFGAADEVGCFQLHDNGIECVADPSGLFRDQRDTPVAGTAVTVALDGKRPLIGEVQALV
GPPTGPHPRRAVSGIDAARAAMIAAVLDKHARLQVGPCDIYLSTVGGMRLTDPSSDLAVAIAMASARKERAMPANSVVIG
ELGLAGDLRRVTGMDRRLAEAARLGFTTAVVPPGTTGVPPGLRVLPAENISAALRVLDRIADNGGR
>Mature_465_residues
AKARSQYRCSECRHITAKWVGRCPDCGTWGTVEEVAVLAAVAGAPARHAVAPASPAVPISSIDPGRTRHDETGISELDRV
LGGGLVPGSVTLLAGDPGVGKSTLLLEVAHRWAVSGRRALYLTGEESAGQVRLRAERTGCTHDELYLAAESDLPTALGHI
DAVQPSLVVVDSVQTMSTSENDGVTGGVTQVRAVTTALTQYAKGAAGPGGPGGIAMVLVGHVTKDGAIAGPRSLEHLVDV
VLHFEGDRNSTLRMVRGVKNRFGAADEVGCFQLHDNGIECVADPSGLFRDQRDTPVAGTAVTVALDGKRPLIGEVQALVG
PPTGPHPRRAVSGIDAARAAMIAAVLDKHARLQVGPCDIYLSTVGGMRLTDPSSDLAVAIAMASARKERAMPANSVVIGE
LGLAGDLRRVTGMDRRLAEAARLGFTTAVVPPGTTGVPPGLRVLPAENISAALRVLDRIADNGGR

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=469, Percent_Identity=39.0191897654584, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48234; Mature: 48102

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKARSQYRCSECRHITAKWVGRCPDCGTWGTVEEVAVLAAVAGAPARHAVAPASPAVPI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSIDPGRTRHDETGISELDRVLGGGLVPGSVTLLAGDPGVGKSTLLLEVAHRWAVSGRRA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
LYLTGEESAGQVRLRAERTGCTHDELYLAAESDLPTALGHIDAVQPSLVVVDSVQTMSTS
EEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECHHHHCCC
ENDGVTGGVTQVRAVTTALTQYAKGAAGPGGPGGIAMVLVGHVTKDGAIAGPRSLEHLVD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
VVLHFEGDRNSTLRMVRGVKNRFGAADEVGCFQLHDNGIECVADPSGLFRDQRDTPVAGT
HHHEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHCCCCCCCCCCCE
AVTVALDGKRPLIGEVQALVGPPTGPHPRRAVSGIDAARAAMIAAVLDKHARLQVGPCDI
EEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
YLSTVGGMRLTDPSSDLAVAIAMASARKERAMPANSVVIGELGLAGDLRRVTGMDRRLAE
EEECCCCEEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
AARLGFTTAVVPPGTTGVPPGLRVLPAENISAALRVLDRIADNGGR
HHHCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKARSQYRCSECRHITAKWVGRCPDCGTWGTVEEVAVLAAVAGAPARHAVAPASPAVPI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSIDPGRTRHDETGISELDRVLGGGLVPGSVTLLAGDPGVGKSTLLLEVAHRWAVSGRRA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
LYLTGEESAGQVRLRAERTGCTHDELYLAAESDLPTALGHIDAVQPSLVVVDSVQTMSTS
EEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECHHHHCCC
ENDGVTGGVTQVRAVTTALTQYAKGAAGPGGPGGIAMVLVGHVTKDGAIAGPRSLEHLVD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
VVLHFEGDRNSTLRMVRGVKNRFGAADEVGCFQLHDNGIECVADPSGLFRDQRDTPVAGT
HHHEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHCCCCCCCCCCCE
AVTVALDGKRPLIGEVQALVGPPTGPHPRRAVSGIDAARAAMIAAVLDKHARLQVGPCDI
EEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
YLSTVGGMRLTDPSSDLAVAIAMASARKERAMPANSVVIGELGLAGDLRRVTGMDRRLAE
EEECCCCEEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
AARLGFTTAVVPPGTTGVPPGLRVLPAENISAALRVLDRIADNGGR
HHHCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]