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The map label for this gene is metG [H]

Identifier: 119870381

GI number: 119870381

Start: 4571490

End: 4573037

Strand: Direct

Name: metG [H]

Synonym: Mkms_4352

Alternate gene names: 119870381

Gene position: 4571490-4573037 (Clockwise)

Preceding gene: 119870373

Following gene: 119870382

Centisome position: 79.68

GC content: 66.54

Gene sequence:

>1548_bases
ATGAGTCAGCCGTTCTACATCACCACCGCGATCGCCTACCCCAACGGCGATCCGCACGTCGGCCACGCCTACGAGTACAT
CGCCACCGATGCGATCGCCCGCTTCAAGCGGCTCGACGGATATGACGTGCGCTACCTGACCGGCACCGACGTGCACGGGC
AGAAGATGGCCGAGACGGCGACGGCGCTCGGTGTGCCCGTGGCCGACCTCGCACGGCGCAACTCCGATGTTTTCCAGCGG
CTGCAGGAGAAGCTCAACATCTCCTTCGACCGGTTCATCCGAACCTCGGACACCGACCACTACCGGGCGTCGGAGGACAT
CTGGCAGCGGATGAACGCCGCCGGCGACATCTATCTCGACGCCTACTCCGGCTGGTACTCGGTGCGCGACGAACGGTTCT
TCACCGAGGGTGAGACGACGCTGCGCGAAGACGGGGTTCGTATCGCCACCGAAACCGGCGCCCCGCTGACGTGGACCGAG
GAGCAGACCTACTTCTTCAAGCTGTCGGCCTACACCGACCGACTGCTCGCCCACTACGAGGCCCATCCCGAGTTCATCCA
GCCCGACGCCCGGCGCAACGAGATCGTCAGCTTCGTCTCCGGGGGGCTGCGCGATCTGTCGATCTCCCGCACCACGTTCG
ACTGGGGGGTGCCGGTCCCCGACCACCCCGACCACGTCATGTACGTGTGGGTGGACGCGCTGACCAACTACCTCACCGGG
GTGGGCTATCCGGACACCTCGTCGGAGGAGTTCCAGCGGTACTGGCCCGCCGATCTGCACATGATCGGCAAGGACATCAT
CCGGTTCCACACCGTCTACTGGCCCGCATTCCTGATGTCGGCGGGACTCGAGTTGCCGCGCAAGGTCTTCGCGCACGGCT
GGCTGCTCAACCGCGGCGAGAAGATGAGCAAGTCGATCGGCAACGTCGTCGACCCGGTGAACCTCGTCGACACCTTCGGC
CTCGACCAGGTGCGCTACTTCTTCCTGCGCGAGGTGCCGTTCGGCCAGGACGGCAGCTACAGCGAGGACGCCATCATCGG
CCGCATCAACGCCGACCTGGCCAACGAGCTGGGCAACCTGGCGCAGCGCTCGCTGTCGATGGTGGCCAAGAACCTCGGCA
GTGCGGTCCCCGAACCCGGCGAGTTCACCGCCGAGGACCTCGCGCTGCTCGACGCCGCCGACGCGCTGATGGACCGGGTC
CGCCGCCATTTCGACGAGCAGGCGATGCATCTGGCGCTGGAGGCGATCTGGTCGGTGCTCGGCGCGGCCAACCGGTACTT
CTCCGCGCAGGAGCCGTGGGTGCTGCGCAAATCGGAGGCCGTCCACGATCAGGTGCGGTTCCGCACCGTGCTCTACACGA
CACTCGAGACCGTGCGGATCGCGGCGTTGCTGAGCCAGCCCGTCATGCCGGATTCGACCGCGACACTGCTCGACCTGGTC
GGCCAACCCGCCGACCAGCGCACCTTCGCCGCCGTTTCGACCCGCCTCACCCCGGGCACCGCACTGCCGAAGCCCGTCGG
GGTGTTTCCCAGATACCAGGTGGATTAG

Upstream 100 bases:

>100_bases
GAGGTGGGTGTGCGCATCGATCAGCGGCGTCAACGGCTCGGGAGCCGGCGGTGCCGGACGGTTCGATCGACTCACGTCCC
ACACCATAAGGTGAACCCAA

Downstream 100 bases:

>100_bases
AGATACAGGTGGCCTGAGCATGAACGGACTGCACGAGAACCTGCAGGGCATCTTCGAAGAGGTGGCGCGGCGCAACCCGG
GCGAGACGGAGTTCCACCAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSQPFYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGYDVRYLTGTDVHGQKMAETATALGVPVADLARRNSDVFQR
LQEKLNISFDRFIRTSDTDHYRASEDIWQRMNAAGDIYLDAYSGWYSVRDERFFTEGETTLREDGVRIATETGAPLTWTE
EQTYFFKLSAYTDRLLAHYEAHPEFIQPDARRNEIVSFVSGGLRDLSISRTTFDWGVPVPDHPDHVMYVWVDALTNYLTG
VGYPDTSSEEFQRYWPADLHMIGKDIIRFHTVYWPAFLMSAGLELPRKVFAHGWLLNRGEKMSKSIGNVVDPVNLVDTFG
LDQVRYFFLREVPFGQDGSYSEDAIIGRINADLANELGNLAQRSLSMVAKNLGSAVPEPGEFTAEDLALLDAADALMDRV
RRHFDEQAMHLALEAIWSVLGAANRYFSAQEPWVLRKSEAVHDQVRFRTVLYTTLETVRIAALLSQPVMPDSTATLLDLV
GQPADQRTFAAVSTRLTPGTALPKPVGVFPRYQVD

Sequences:

>Translated_515_residues
MSQPFYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGYDVRYLTGTDVHGQKMAETATALGVPVADLARRNSDVFQR
LQEKLNISFDRFIRTSDTDHYRASEDIWQRMNAAGDIYLDAYSGWYSVRDERFFTEGETTLREDGVRIATETGAPLTWTE
EQTYFFKLSAYTDRLLAHYEAHPEFIQPDARRNEIVSFVSGGLRDLSISRTTFDWGVPVPDHPDHVMYVWVDALTNYLTG
VGYPDTSSEEFQRYWPADLHMIGKDIIRFHTVYWPAFLMSAGLELPRKVFAHGWLLNRGEKMSKSIGNVVDPVNLVDTFG
LDQVRYFFLREVPFGQDGSYSEDAIIGRINADLANELGNLAQRSLSMVAKNLGSAVPEPGEFTAEDLALLDAADALMDRV
RRHFDEQAMHLALEAIWSVLGAANRYFSAQEPWVLRKSEAVHDQVRFRTVLYTTLETVRIAALLSQPVMPDSTATLLDLV
GQPADQRTFAAVSTRLTPGTALPKPVGVFPRYQVD
>Mature_514_residues
SQPFYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGYDVRYLTGTDVHGQKMAETATALGVPVADLARRNSDVFQRL
QEKLNISFDRFIRTSDTDHYRASEDIWQRMNAAGDIYLDAYSGWYSVRDERFFTEGETTLREDGVRIATETGAPLTWTEE
QTYFFKLSAYTDRLLAHYEAHPEFIQPDARRNEIVSFVSGGLRDLSISRTTFDWGVPVPDHPDHVMYVWVDALTNYLTGV
GYPDTSSEEFQRYWPADLHMIGKDIIRFHTVYWPAFLMSAGLELPRKVFAHGWLLNRGEKMSKSIGNVVDPVNLVDTFGL
DQVRYFFLREVPFGQDGSYSEDAIIGRINADLANELGNLAQRSLSMVAKNLGSAVPEPGEFTAEDLALLDAADALMDRVR
RHFDEQAMHLALEAIWSVLGAANRYFSAQEPWVLRKSEAVHDQVRFRTVLYTTLETVRIAALLSQPVMPDSTATLLDLVG
QPADQRTFAAVSTRLTPGTALPKPVGVFPRYQVD

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=36.0623781676413, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=26.9230769230769, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788432, Length=510, Percent_Identity=25.4901960784314, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI212645096, Length=528, Percent_Identity=33.7121212121212, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI71992781, Length=419, Percent_Identity=32.6968973747017, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17540794, Length=517, Percent_Identity=24.9516441005803, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321610, Length=536, Percent_Identity=32.2761194029851, Blast_Score=286, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6321704, Length=526, Percent_Identity=24.1444866920152, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358483, Length=529, Percent_Identity=37.8071833648393, Blast_Score=348, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=25.5772646536412, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58150; Mature: 58019

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPFYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGYDVRYLTGTDVHGQKMAETA
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
TALGVPVADLARRNSDVFQRLQEKLNISFDRFIRTSDTDHYRASEDIWQRMNAAGDIYLD
HHHCCCHHHHHHCCHHHHHHHHHHHCCCHHHHEECCCCCCCCCHHHHHHHHCCCCCEEEE
AYSGWYSVRDERFFTEGETTLREDGVRIATETGAPLTWTEEQTYFFKLSAYTDRLLAHYE
ECCCCHHHCCCEEECCCCHHHHHCCCEEEECCCCCEEEECCCEEEEEEHHHHHHHHHHHH
AHPEFIQPDARRNEIVSFVSGGLRDLSISRTTFDWGVPVPDHPDHVMYVWVDALTNYLTG
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHC
VGYPDTSSEEFQRYWPADLHMIGKDIIRFHTVYWPAFLMSAGLELPRKVFAHGWLLNRGE
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHCCCH
KMSKSIGNVVDPVNLVDTFGLDQVRYFFLREVPFGQDGSYSEDAIIGRINADLANELGNL
HHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AQRSLSMVAKNLGSAVPEPGEFTAEDLALLDAADALMDRVRRHFDEQAMHLALEAIWSVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAANRYFSAQEPWVLRKSEAVHDQVRFRTVLYTTLETVRIAALLSQPVMPDSTATLLDLV
HHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GQPADQRTFAAVSTRLTPGTALPKPVGVFPRYQVD
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQPFYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGYDVRYLTGTDVHGQKMAETA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
TALGVPVADLARRNSDVFQRLQEKLNISFDRFIRTSDTDHYRASEDIWQRMNAAGDIYLD
HHHCCCHHHHHHCCHHHHHHHHHHHCCCHHHHEECCCCCCCCCHHHHHHHHCCCCCEEEE
AYSGWYSVRDERFFTEGETTLREDGVRIATETGAPLTWTEEQTYFFKLSAYTDRLLAHYE
ECCCCHHHCCCEEECCCCHHHHHCCCEEEECCCCCEEEECCCEEEEEEHHHHHHHHHHHH
AHPEFIQPDARRNEIVSFVSGGLRDLSISRTTFDWGVPVPDHPDHVMYVWVDALTNYLTG
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHC
VGYPDTSSEEFQRYWPADLHMIGKDIIRFHTVYWPAFLMSAGLELPRKVFAHGWLLNRGE
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHCCCH
KMSKSIGNVVDPVNLVDTFGLDQVRYFFLREVPFGQDGSYSEDAIIGRINADLANELGNL
HHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AQRSLSMVAKNLGSAVPEPGEFTAEDLALLDAADALMDRVRRHFDEQAMHLALEAIWSVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAANRYFSAQEPWVLRKSEAVHDQVRFRTVLYTTLETVRIAALLSQPVMPDSTATLLDLV
HHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GQPADQRTFAAVSTRLTPGTALPKPVGVFPRYQVD
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]