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The map label for this gene is 119870373

Identifier: 119870373

GI number: 119870373

Start: 4562219

End: 4563640

Strand: Direct

Name: 119870373

Synonym: Mkms_4344

Alternate gene names: NA

Gene position: 4562219-4563640 (Clockwise)

Preceding gene: 119870372

Following gene: 119870381

Centisome position: 79.52

GC content: 69.83

Gene sequence:

>1422_bases
ATGGACCTTCTGGATCCGCTCGACGCGGCGATGATGACCGGAGAGGTGATCGCCAGCCCCCTGCACGTCTCCGCGGTGCT
GATCCTGGAACCGCCCGCCGATGCCGGGCCGGGATACCTCGACGAGGTCTACCAGCGGGCGCGCACTCCCTCGGTCGCCG
TGGACCGGCGGCTTCGTCGCTATCCCCACGTCGGCACCGACACCGGTGGATTGTGGACCTGGCGCGAGACCGACACCGTT
GACCTGGGCCGGCATGTCACGCGCCGGACACTGCCCGCCGGTTCAGGCCGAAGCGCACTGTGGCACCTGGTGGGCCGGCT
GCACGCCGAACGCCTCGACCGATCCGGCCCGATGTGGTCCGGGTGTCTGATCGACGGCCTCGACGACGGGCGCTTCGCCT
GGTTCGTGAAGGTGCACCACACCGTCATGGACGGGGTCGCGGGCCTTCAGCTGATCATCGACGGTTTGAGCGCCGACCCG
GACCGCCGCGACATGCCGCCGTTCTACGCCGCCTCCGGCGCGGCGGCCGGCCGGTCGAACCCTGTTCGTTCCCCCGGCCC
CTTGGGGCAGCTGCGGTCGATCGTCGGCGCCAGCGCGTCGAGCGTGGAGCTGACGGGACGTCTCGTCGGTGGCGAGCTCT
CCGATCTGGTGCACTACGTCACCGGCGACACCACCGTGGCGCCGTTGACTGCTCCCTACACGCGGCTCAACCACCGGCTC
GGTGACGAACGCGCCGTCGCGGCGGCCACCGTGGCCAAGACCCGCATCAAGGCCGTCCAGTCCCGGGCGGGAGTGACCGG
CAATGACGTCGTCACCGCCGTTGTAGCCGGGGTCTTGAGAGGCTGGCTGATGGATCACGATGAGCTGCCAGGCCGCTCAC
TCGTGGCGCTCTGCCCGATCACCGTGCGGGGCCGCGAACACGGAACCCCCGAGAGCGGCAACGCGTTCGGCGGATGGCTG
TGTCCGCTGGGCACCGACATGGACGATCCCGCCGCCCGACTGGATCTGATCCACCGGTCGATGGCCGAGGGTAAACATCA
GGTCGGGAAGCGGGGCGCCGGCACCTCCATGCTCCTGCTGGCGCTGAGCATCGCACCGACCCTGCTGACGCCGCTGCTGC
CGCTCGTGCCGCGGCTGCGTACCGGGTACAACCTGCCGATCTCCAACGTCCCGGGGCCGCGCACCGAAATGTATTGGAAC
GGAGCCCATGTCGAAGAGATCTACCCGGTGTCGGCGGTGTACGGCGGGCAGGCGCTCAACGTCACGGTGTGCTCCTACGC
GGACCGGGTCTGCTTCGGCTGGGTGGCCGGGGCCGAGGTGATGCCCGACATCGCCACGGTGACAGAACGTACCGAGCGGT
CACTGGCCGAACTGGAAGCAGCGGTTGGCGCGCGCGGCTACCCCCCCGTGCGCCTCGCGTAA

Upstream 100 bases:

>100_bases
ACCGGGTTCTTCCAGGCGCGGCCCGTGCAGCGGTTCGTCGAGGCCGCCCTCGTCCAGTCGGGCCTCGTGGACGGAGCTGC
ACGGTCTCGGGAGAGGTGAC

Downstream 100 bases:

>100_bases
GCGCGCAGGGCGAACGGGGCGAACACCGCCGTCAGCACCAGGGACCACAGCACCGTCGCCAGCACCGGGTGGTGCAGCGG
CAACTGCGCATCCGGTGCCG

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3740c/MT3848 [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDLLDPLDAAMMTGEVIASPLHVSAVLILEPPADAGPGYLDEVYQRARTPSVAVDRRLRRYPHVGTDTGGLWTWRETDTV
DLGRHVTRRTLPAGSGRSALWHLVGRLHAERLDRSGPMWSGCLIDGLDDGRFAWFVKVHHTVMDGVAGLQLIIDGLSADP
DRRDMPPFYAASGAAAGRSNPVRSPGPLGQLRSIVGASASSVELTGRLVGGELSDLVHYVTGDTTVAPLTAPYTRLNHRL
GDERAVAAATVAKTRIKAVQSRAGVTGNDVVTAVVAGVLRGWLMDHDELPGRSLVALCPITVRGREHGTPESGNAFGGWL
CPLGTDMDDPAARLDLIHRSMAEGKHQVGKRGAGTSMLLLALSIAPTLLTPLLPLVPRLRTGYNLPISNVPGPRTEMYWN
GAHVEEIYPVSAVYGGQALNVTVCSYADRVCFGWVAGAEVMPDIATVTERTERSLAELEAAVGARGYPPVRLA

Sequences:

>Translated_473_residues
MDLLDPLDAAMMTGEVIASPLHVSAVLILEPPADAGPGYLDEVYQRARTPSVAVDRRLRRYPHVGTDTGGLWTWRETDTV
DLGRHVTRRTLPAGSGRSALWHLVGRLHAERLDRSGPMWSGCLIDGLDDGRFAWFVKVHHTVMDGVAGLQLIIDGLSADP
DRRDMPPFYAASGAAAGRSNPVRSPGPLGQLRSIVGASASSVELTGRLVGGELSDLVHYVTGDTTVAPLTAPYTRLNHRL
GDERAVAAATVAKTRIKAVQSRAGVTGNDVVTAVVAGVLRGWLMDHDELPGRSLVALCPITVRGREHGTPESGNAFGGWL
CPLGTDMDDPAARLDLIHRSMAEGKHQVGKRGAGTSMLLLALSIAPTLLTPLLPLVPRLRTGYNLPISNVPGPRTEMYWN
GAHVEEIYPVSAVYGGQALNVTVCSYADRVCFGWVAGAEVMPDIATVTERTERSLAELEAAVGARGYPPVRLA
>Mature_473_residues
MDLLDPLDAAMMTGEVIASPLHVSAVLILEPPADAGPGYLDEVYQRARTPSVAVDRRLRRYPHVGTDTGGLWTWRETDTV
DLGRHVTRRTLPAGSGRSALWHLVGRLHAERLDRSGPMWSGCLIDGLDDGRFAWFVKVHHTVMDGVAGLQLIIDGLSADP
DRRDMPPFYAASGAAAGRSNPVRSPGPLGQLRSIVGASASSVELTGRLVGGELSDLVHYVTGDTTVAPLTAPYTRLNHRL
GDERAVAAATVAKTRIKAVQSRAGVTGNDVVTAVVAGVLRGWLMDHDELPGRSLVALCPITVRGREHGTPESGNAFGGWL
CPLGTDMDDPAARLDLIHRSMAEGKHQVGKRGAGTSMLLLALSIAPTLLTPLLPLVPRLRTGYNLPISNVPGPRTEMYWN
GAHVEEIYPVSAVYGGQALNVTVCSYADRVCFGWVAGAEVMPDIATVTERTERSLAELEAAVGARGYPPVRLA

Specific function: Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 50498; Mature: 50498

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00052 RIBOSOMAL_S7

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLDPLDAAMMTGEVIASPLHVSAVLILEPPADAGPGYLDEVYQRARTPSVAVDRRLRR
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
YPHVGTDTGGLWTWRETDTVDLGRHVTRRTLPAGSGRSALWHLVGRLHAERLDRSGPMWS
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GCLIDGLDDGRFAWFVKVHHTVMDGVAGLQLIIDGLSADPDRRDMPPFYAASGAAAGRSN
CEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PVRSPGPLGQLRSIVGASASSVELTGRLVGGELSDLVHYVTGDTTVAPLTAPYTRLNHRL
CCCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHHHC
GDERAVAAATVAKTRIKAVQSRAGVTGNDVVTAVVAGVLRGWLMDHDELPGRSLVALCPI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCE
TVRGREHGTPESGNAFGGWLCPLGTDMDDPAARLDLIHRSMAEGKHQVGKRGAGTSMLLL
EEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALSIAPTLLTPLLPLVPRLRTGYNLPISNVPGPRTEMYWNGAHVEEIYPVSAVYGGQALN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHCCHHHEECCCEEE
VTVCSYADRVCFGWVAGAEVMPDIATVTERTERSLAELEAAVGARGYPPVRLA
EEHHHHHCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDLLDPLDAAMMTGEVIASPLHVSAVLILEPPADAGPGYLDEVYQRARTPSVAVDRRLRR
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
YPHVGTDTGGLWTWRETDTVDLGRHVTRRTLPAGSGRSALWHLVGRLHAERLDRSGPMWS
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GCLIDGLDDGRFAWFVKVHHTVMDGVAGLQLIIDGLSADPDRRDMPPFYAASGAAAGRSN
CEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PVRSPGPLGQLRSIVGASASSVELTGRLVGGELSDLVHYVTGDTTVAPLTAPYTRLNHRL
CCCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHHHC
GDERAVAAATVAKTRIKAVQSRAGVTGNDVVTAVVAGVLRGWLMDHDELPGRSLVALCPI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCE
TVRGREHGTPESGNAFGGWLCPLGTDMDDPAARLDLIHRSMAEGKHQVGKRGAGTSMLLL
EEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALSIAPTLLTPLLPLVPRLRTGYNLPISNVPGPRTEMYWNGAHVEEIYPVSAVYGGQALN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHCCHHHEECCCEEE
VTVCSYADRVCFGWVAGAEVMPDIATVTERTERSLAELEAAVGARGYPPVRLA
EEHHHHHCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]