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The map label for this gene is rsbU [H]

Identifier: 119870004

GI number: 119870004

Start: 4189064

End: 4191049

Strand: Direct

Name: rsbU [H]

Synonym: Mkms_3974

Alternate gene names: 119870004

Gene position: 4189064-4191049 (Clockwise)

Preceding gene: 119869996

Following gene: 119870005

Centisome position: 73.02

GC content: 70.75

Gene sequence:

>1986_bases
GTGGATCCTGATCAGGGGGCGCTGGGAGATCACCGGGTTCGCTCACGAAAACCGGCCGTGGTGTTCGTCGTCGGGTGTCT
GTTCGCCGTCCTCGTCGTCGGGTGGCTATCGCTGGTGACCCAGCCGACGGCGCTGGCCAGCACGGCGTGGTGGCCCGTCG
CCGGGATCGCGGTCGGGTTGGGCATCCGCTTCCCGCGCCGTCAGGTGTGGGCGCCGGCCGCCGCCGTCGCCGCGATCACC
CTTCCGCTCCTGCTCTGGGCGGGACGGCCCGCTGCGCTCGCGACCGCGCTCGCATTCGCCGTCGCCCTCGAGATGGTGAT
CGCCACCCTCATCCTCAGGGGGCGCCACGATCGCCTGCCGAGCCTGTCGGAACCGCGCGACCTCGGCCGGTTACTGGTGG
CCGTCGCCTCGGCGGCGATCGTCTACGACGTGGTCGGCGCCGGCGCCACCTACCTGCTCGCCGATTCCACCGAGGCGTGG
ATCCGGTTCGTCACGTCCGCGCCGAAGCACGCGGCCGGGATGCTGCTGCTGGTCCCGCTGTTCATGCACCTGCCGCGCCG
CCCCCGGCCTGCGGGTCCGGTCGAGACCGTCGCGCAGGTCGTGACGACCCTGGGCCTGGTCACCTTCGTGTTCGCGTTCA
ACCCCGGAATGCCGCTGTCCTTCCTCCCCTTCATGCCACTGGTGTGGGTGGCGATCCGACTGACGACCCGGGAACTGATC
CTGCTGATGCTGGCGATCGCCGTCATCGCCTCGGCCGGCAGCGCGTACGGCACCGGGCCGTTCGCGTTCAACCTCCTCGC
ACCGGAGGTGGGCAACCTCGTCCTGCAGGTCTTCGAGCTGTCGATGGTGGTCGTCTTCCTCTCGCTCTCGCTCGCGGTCG
GCCACGAGCGGACCACCGCACGGCGCCTCAACGAGAGCGAGGAGTTGTTCCGCCGGATCTTCGAAACGTCGGTGGCCGGG
ATGCTGATCGCCACCCGTGCCGCCACGGGATGGAAGGTGTTGCGCGCCAACGACTCCGCGGTGGCCATCATCCCCGGTCT
CGCCGACGCGTCGGCCGAGCTCACCGATCTGTTGGGCGAGGAGGCCACCGCCGCGCTCTCGGCGGAAGCCGACGCGCTCA
CCGAGGGCAACGCGCGCCTGACGCTGACCACCGGCACCGAGCGGATCCTCAACGTCAGCATCTCCCCGATCAGCGTCGAC
GGGGACAGCAGGACCCTCGCGCTGCAGTTCTACGACATCACCGAGGCGATGCGCGCCCGCAGGCTGGAACAGGAGGAACT
CGAGCGCGCCGCCGAAGTGCAACGTGCCCTCCTGCCCGGGACGCTCCCACCCACCCCCGGGTGGACTTCCGGTGCGGCTT
CGGTGCCGGCCAGACAGGTCGGCGGGGACTTCTACGACATCCGGGTCCAGGTCCCGCACGTGGTCCTCAGCCTCGGCGAC
GTCATGGGTAAGGGCATGGGCGCGGGAATGTTGGCCGCCGCGACCAGAGCCGCGCTGCGCGCCACCGACCCCGAGCTCAG
TCCATCGGCCGCCGTGAGCCACATGGCCGGGGTCGTCGATCACGACCTGCAACGCACCAGCGCCTTCATCACGCTGACCT
ACGTCCTCGTCGACCTCGTCACCGGCGACTTCCGCGTCGCCGACGCCGGGCACGGACTGCACTTCGTCGTCCGGACCGGA
TCGGGTCTGGTGGAGCGCACCGCCTCCAGCGATATGCCGGTGGGGCTCGACAGCGGCTGGGGCGAGAAGCGCGGAGCGCT
CCAGCCCGGCGACGCGATCCTCCTCGTCAGCGACGGCGTGATGGACCTGTGGGGCGGCTCCGTCGAAGAGCTGTCGGATG
CCGTGGCACAGTGCGCCCGGCAGCACGGCACGAGCCCGCAGGCGTTGGTCGACGCCCTGTGCGCGCGGGCGAACGGTGAT
CTGGACCGCGATGATGTGACGGCCGTCGTCCTGCGGCGGGAACCGGTGGACGTGGCGGCACGCTGA

Upstream 100 bases:

>100_bases
GACGAATTCCGGTGAGTTAACTACGCCAGGACGCGCCCGCACGTCGACCCGGTCGCCCACTCGACCGCATTCGCGTTGAT
CAGCGTGTAAAAGTGAGCGG

Downstream 100 bases:

>100_bases
CCGCGAACTTCGAACCATCCTGCCGTGAGTTTGCTGAACACCGACACAGTTGCGTTTGTTCGCAAAGATCGGTGCCCACC
CGAAAGGGAGGCATTTGCCC

Product: stage II sporulation E family protein

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MDPDQGALGDHRVRSRKPAVVFVVGCLFAVLVVGWLSLVTQPTALASTAWWPVAGIAVGLGIRFPRRQVWAPAAAVAAIT
LPLLLWAGRPAALATALAFAVALEMVIATLILRGRHDRLPSLSEPRDLGRLLVAVASAAIVYDVVGAGATYLLADSTEAW
IRFVTSAPKHAAGMLLLVPLFMHLPRRPRPAGPVETVAQVVTTLGLVTFVFAFNPGMPLSFLPFMPLVWVAIRLTTRELI
LLMLAIAVIASAGSAYGTGPFAFNLLAPEVGNLVLQVFELSMVVVFLSLSLAVGHERTTARRLNESEELFRRIFETSVAG
MLIATRAATGWKVLRANDSAVAIIPGLADASAELTDLLGEEATAALSAEADALTEGNARLTLTTGTERILNVSISPISVD
GDSRTLALQFYDITEAMRARRLEQEELERAAEVQRALLPGTLPPTPGWTSGAASVPARQVGGDFYDIRVQVPHVVLSLGD
VMGKGMGAGMLAAATRAALRATDPELSPSAAVSHMAGVVDHDLQRTSAFITLTYVLVDLVTGDFRVADAGHGLHFVVRTG
SGLVERTASSDMPVGLDSGWGEKRGALQPGDAILLVSDGVMDLWGGSVEELSDAVAQCARQHGTSPQALVDALCARANGD
LDRDDVTAVVLRREPVDVAAR

Sequences:

>Translated_661_residues
MDPDQGALGDHRVRSRKPAVVFVVGCLFAVLVVGWLSLVTQPTALASTAWWPVAGIAVGLGIRFPRRQVWAPAAAVAAIT
LPLLLWAGRPAALATALAFAVALEMVIATLILRGRHDRLPSLSEPRDLGRLLVAVASAAIVYDVVGAGATYLLADSTEAW
IRFVTSAPKHAAGMLLLVPLFMHLPRRPRPAGPVETVAQVVTTLGLVTFVFAFNPGMPLSFLPFMPLVWVAIRLTTRELI
LLMLAIAVIASAGSAYGTGPFAFNLLAPEVGNLVLQVFELSMVVVFLSLSLAVGHERTTARRLNESEELFRRIFETSVAG
MLIATRAATGWKVLRANDSAVAIIPGLADASAELTDLLGEEATAALSAEADALTEGNARLTLTTGTERILNVSISPISVD
GDSRTLALQFYDITEAMRARRLEQEELERAAEVQRALLPGTLPPTPGWTSGAASVPARQVGGDFYDIRVQVPHVVLSLGD
VMGKGMGAGMLAAATRAALRATDPELSPSAAVSHMAGVVDHDLQRTSAFITLTYVLVDLVTGDFRVADAGHGLHFVVRTG
SGLVERTASSDMPVGLDSGWGEKRGALQPGDAILLVSDGVMDLWGGSVEELSDAVAQCARQHGTSPQALVDALCARANGD
LDRDDVTAVVLRREPVDVAAR
>Mature_661_residues
MDPDQGALGDHRVRSRKPAVVFVVGCLFAVLVVGWLSLVTQPTALASTAWWPVAGIAVGLGIRFPRRQVWAPAAAVAAIT
LPLLLWAGRPAALATALAFAVALEMVIATLILRGRHDRLPSLSEPRDLGRLLVAVASAAIVYDVVGAGATYLLADSTEAW
IRFVTSAPKHAAGMLLLVPLFMHLPRRPRPAGPVETVAQVVTTLGLVTFVFAFNPGMPLSFLPFMPLVWVAIRLTTRELI
LLMLAIAVIASAGSAYGTGPFAFNLLAPEVGNLVLQVFELSMVVVFLSLSLAVGHERTTARRLNESEELFRRIFETSVAG
MLIATRAATGWKVLRANDSAVAIIPGLADASAELTDLLGEEATAALSAEADALTEGNARLTLTTGTERILNVSISPISVD
GDSRTLALQFYDITEAMRARRLEQEELERAAEVQRALLPGTLPPTPGWTSGAASVPARQVGGDFYDIRVQVPHVVLSLGD
VMGKGMGAGMLAAATRAALRATDPELSPSAAVSHMAGVVDHDLQRTSAFITLTYVLVDLVTGDFRVADAGHGLHFVVRTG
SGLVERTASSDMPVGLDSGWGEKRGALQPGDAILLVSDGVMDLWGGSVEELSDAVAQCARQHGTSPQALVDALCARANGD
LDRDDVTAVVLRREPVDVAAR

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 69818; Mature: 69818

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPDQGALGDHRVRSRKPAVVFVVGCLFAVLVVGWLSLVTQPTALASTAWWPVAGIAVGL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
GIRFPRRQVWAPAAAVAAITLPLLLWAGRPAALATALAFAVALEMVIATLILRGRHDRLP
CCCCCCHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SLSEPRDLGRLLVAVASAAIVYDVVGAGATYLLADSTEAWIRFVTSAPKHAAGMLLLVPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHH
FMHLPRRPRPAGPVETVAQVVTTLGLVTFVFAFNPGMPLSFLPFMPLVWVAIRLTTRELI
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLMLAIAVIASAGSAYGTGPFAFNLLAPEVGNLVLQVFELSMVVVFLSLSLAVGHERTTA
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRLNESEELFRRIFETSVAGMLIATRAATGWKVLRANDSAVAIIPGLADASAELTDLLGE
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHH
EATAALSAEADALTEGNARLTLTTGTERILNVSISPISVDGDSRTLALQFYDITEAMRAR
HHHHHHHCCCHHHCCCCCEEEEEECCCEEEEEEECEEEECCCCCEEEEEEHHHHHHHHHH
RLEQEELERAAEVQRALLPGTLPPTPGWTSGAASVPARQVGGDFYDIRVQVPHVVLSLGD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEHHHHHHHHHH
VMGKGMGAGMLAAATRAALRATDPELSPSAAVSHMAGVVDHDLQRTSAFITLTYVLVDLV
HHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGDFRVADAGHGLHFVVRTGSGLVERTASSDMPVGLDSGWGEKRGALQPGDAILLVSDGV
CCCEEEEECCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
MDLWGGSVEELSDAVAQCARQHGTSPQALVDALCARANGDLDRDDVTAVVLRREPVDVAA
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
R
C
>Mature Secondary Structure
MDPDQGALGDHRVRSRKPAVVFVVGCLFAVLVVGWLSLVTQPTALASTAWWPVAGIAVGL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
GIRFPRRQVWAPAAAVAAITLPLLLWAGRPAALATALAFAVALEMVIATLILRGRHDRLP
CCCCCCHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SLSEPRDLGRLLVAVASAAIVYDVVGAGATYLLADSTEAWIRFVTSAPKHAAGMLLLVPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHH
FMHLPRRPRPAGPVETVAQVVTTLGLVTFVFAFNPGMPLSFLPFMPLVWVAIRLTTRELI
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLMLAIAVIASAGSAYGTGPFAFNLLAPEVGNLVLQVFELSMVVVFLSLSLAVGHERTTA
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRLNESEELFRRIFETSVAGMLIATRAATGWKVLRANDSAVAIIPGLADASAELTDLLGE
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHH
EATAALSAEADALTEGNARLTLTTGTERILNVSISPISVDGDSRTLALQFYDITEAMRAR
HHHHHHHCCCHHHCCCCCEEEEEECCCEEEEEEECEEEECCCCCEEEEEEHHHHHHHHHH
RLEQEELERAAEVQRALLPGTLPPTPGWTSGAASVPARQVGGDFYDIRVQVPHVVLSLGD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEHHHHHHHHHH
VMGKGMGAGMLAAATRAALRATDPELSPSAAVSHMAGVVDHDLQRTSAFITLTYVLVDLV
HHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGDFRVADAGHGLHFVVRTGSGLVERTASSDMPVGLDSGWGEKRGALQPGDAILLVSDGV
CCCEEEEECCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
MDLWGGSVEELSDAVAQCARQHGTSPQALVDALCARANGDLDRDDVTAVVLRREPVDVAA
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]