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The map label for this gene is sir [H]

Identifier: 119869569

GI number: 119869569

Start: 3729719

End: 3731401

Strand: Direct

Name: sir [H]

Synonym: Mkms_3537

Alternate gene names: 119869569

Gene position: 3729719-3731401 (Clockwise)

Preceding gene: 119869566

Following gene: 119869570

Centisome position: 65.01

GC content: 67.02

Gene sequence:

>1683_bases
ATGACCGAGACCCCGACCCGTCCCGCCAAGCCCGTGAAGCGTCCGCGCGGCGAAGGACAGTGGGCACTGGGATACCGCGA
GCCGCTCAACGCCAACGAGCAGTCCAAGAAGGACGACAACCCGCTCAACGTGCGGGAGCGGATCGAGAACATCTACGCCC
CCGGCGGCTTCGACACCATCGACAAGGGCGACCTGCGCGGCCGCTTCCGTTGGTGGGGCCTCTACACCCAACGCAAGCCC
GGGTTCGACGGCACGTGGACCGGTGACGAGAACACCGACATGCTCGAAGACGAGTACTTCATGCTGCGGGTTCGCAGCGA
CGGCGGTGCGCTGACCGCGAAGGCGCTGCGCACCCTCGGTGAGATCTCCACCGAGTTCGCCAGGGACACCGCCGACATCT
CCGACCGGCAGAACGTCCAGTACCACTGGATCGACGTCAGGAACATGCCGGAGATCTGGCGCCGGCTCGACGAGGTGGGG
TTGCAGACCACAGAGGCGTGCGGCGACTGCCCCCGCGTCGTGCTCGGTTCGCCGCTGGCCGGTGAGTCCCTCGACGAGGT
GATCGACGGTACGCCCGCGGTCAACGAGATCGTCAAGCGCTACATCGGCAAGCCGGAGTACTCCAACCTGCCGCGCAAGT
TCAAGACCGCGATCTCCGGTCTGCAGGACGTCGTGCACGAGGTCAACGACGTCGCGTTCATCGGTGTGGTGCATCCCGAG
CACGGCCCCGGCTTCGACCTGTGGGTCGGCGGTGGGCTGTCCACCAACCCGATGCTGGGCCAGCGCGTCGGGGCCTGGGT
TCCGCTCGACGAGGTGCCCGACGTCTGGGAGGGCGTGGTCAGCGTGTTCCGCGACTACGGCTATCGGCGGTTGCGGTCGA
AGGCCCGGCTGAAGTTCCTGATCAAGGACTGGGGCGTAGAGAAATTCCGCCAGGTTCTCGAGACGGAGTACCTCAAGCGC
CCGCTCATCGATGGACCCGCGCCCGACCCCGTGACCCGTCCGATCGACCACGTCGGGGTGCAGAAGCTCAAGAACGGTCT
CAACGCCGTCGGCGTCGCCCCCATCGCCGGCCGCGTGTCGGGCACCATCCTGAGCAAGGTCGCCGACCTGGCCGAGGCGG
CGGGTTCCGACCGGATCCGGTTCACGCCGTACCAGAAGCTGATCGTGCTGGACGTGCCCGACGAGAAGCTCGAGGAGCTG
CGCGCCGGACTGGACGCGTTGGGCCTGCCCTCGACGCCGTCGCACTGGCGGCGAAACCTGATGGCGTGCACGGGAATCGA
GTTCTGCAAGCTCAGCTTCGCCGAGACCAGGAGCCGCTCGCAGGTGCTGGTGCCCGAGCTCGAGAAGCGCCTCGAGGACA
TCAACGCCCAGCTCGACGTGCCGGTGACGATCAACATCAACGGCTGCCCGAACTCGTGCGCCCGTATCCAGGTCGCCGAC
ATCGGGTTCAAGGGCCAGATGGTGGACGACGGAGACGGCCCCCAAGAGGGCTTCCAGGTCCACCTGGGCGGCAGCCTCGG
GCTGGACAGCGGTTTCGGCCGCAAGCTGCGCCAGCACAAGGTGCTCGCCACCGAACTCGGCGACTACATCGAGCGGGTGG
TTCGCAACTTCGTGAAACAACGCGAGGACGGCGAGCGTTTCGCAACGTGGGCCCTACGAGCAGACGAGGCGGACTTGCGG
TGA

Upstream 100 bases:

>100_bases
ACCCCGCACGTACAGCTGGCCGCCGGATCTGCGCCGCCGGACAGCCCCCCGGTGATTCAGCGCGGTCCCAACGCCGGCTC
CCAGCAAGGAGCCAGACCCC

Downstream 100 bases:

>100_bases
CGGATGTGATGTCGGATATCGACCTGCAGCAACTGGCGGAGCGCGGCGCGGCCGAACTCGGCCCGAACGCGTCGGCGATC
GACCTGCTGCGCTGGACCGA

Product: ferredoxin--nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTETPTRPAKPVKRPRGEGQWALGYREPLNANEQSKKDDNPLNVRERIENIYAPGGFDTIDKGDLRGRFRWWGLYTQRKP
GFDGTWTGDENTDMLEDEYFMLRVRSDGGALTAKALRTLGEISTEFARDTADISDRQNVQYHWIDVRNMPEIWRRLDEVG
LQTTEACGDCPRVVLGSPLAGESLDEVIDGTPAVNEIVKRYIGKPEYSNLPRKFKTAISGLQDVVHEVNDVAFIGVVHPE
HGPGFDLWVGGGLSTNPMLGQRVGAWVPLDEVPDVWEGVVSVFRDYGYRRLRSKARLKFLIKDWGVEKFRQVLETEYLKR
PLIDGPAPDPVTRPIDHVGVQKLKNGLNAVGVAPIAGRVSGTILSKVADLAEAAGSDRIRFTPYQKLIVLDVPDEKLEEL
RAGLDALGLPSTPSHWRRNLMACTGIEFCKLSFAETRSRSQVLVPELEKRLEDINAQLDVPVTININGCPNSCARIQVAD
IGFKGQMVDDGDGPQEGFQVHLGGSLGLDSGFGRKLRQHKVLATELGDYIERVVRNFVKQREDGERFATWALRADEADLR

Sequences:

>Translated_560_residues
MTETPTRPAKPVKRPRGEGQWALGYREPLNANEQSKKDDNPLNVRERIENIYAPGGFDTIDKGDLRGRFRWWGLYTQRKP
GFDGTWTGDENTDMLEDEYFMLRVRSDGGALTAKALRTLGEISTEFARDTADISDRQNVQYHWIDVRNMPEIWRRLDEVG
LQTTEACGDCPRVVLGSPLAGESLDEVIDGTPAVNEIVKRYIGKPEYSNLPRKFKTAISGLQDVVHEVNDVAFIGVVHPE
HGPGFDLWVGGGLSTNPMLGQRVGAWVPLDEVPDVWEGVVSVFRDYGYRRLRSKARLKFLIKDWGVEKFRQVLETEYLKR
PLIDGPAPDPVTRPIDHVGVQKLKNGLNAVGVAPIAGRVSGTILSKVADLAEAAGSDRIRFTPYQKLIVLDVPDEKLEEL
RAGLDALGLPSTPSHWRRNLMACTGIEFCKLSFAETRSRSQVLVPELEKRLEDINAQLDVPVTININGCPNSCARIQVAD
IGFKGQMVDDGDGPQEGFQVHLGGSLGLDSGFGRKLRQHKVLATELGDYIERVVRNFVKQREDGERFATWALRADEADLR
>Mature_559_residues
TETPTRPAKPVKRPRGEGQWALGYREPLNANEQSKKDDNPLNVRERIENIYAPGGFDTIDKGDLRGRFRWWGLYTQRKPG
FDGTWTGDENTDMLEDEYFMLRVRSDGGALTAKALRTLGEISTEFARDTADISDRQNVQYHWIDVRNMPEIWRRLDEVGL
QTTEACGDCPRVVLGSPLAGESLDEVIDGTPAVNEIVKRYIGKPEYSNLPRKFKTAISGLQDVVHEVNDVAFIGVVHPEH
GPGFDLWVGGGLSTNPMLGQRVGAWVPLDEVPDVWEGVVSVFRDYGYRRLRSKARLKFLIKDWGVEKFRQVLETEYLKRP
LIDGPAPDPVTRPIDHVGVQKLKNGLNAVGVAPIAGRVSGTILSKVADLAEAAGSDRIRFTPYQKLIVLDVPDEKLEELR
AGLDALGLPSTPSHWRRNLMACTGIEFCKLSFAETRSRSQVLVPELEKRLEDINAQLDVPVTININGCPNSCARIQVADI
GFKGQMVDDGDGPQEGFQVHLGGSLGLDSGFGRKLRQHKVLATELGDYIERVVRNFVKQREDGERFATWALRADEADLR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=541, Percent_Identity=25.5083179297597, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322597, Length=548, Percent_Identity=27.1897810218978, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62578; Mature: 62446

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPTRPAKPVKRPRGEGQWALGYREPLNANEQSKKDDNPLNVRERIENIYAPGGFDTI
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
DKGDLRGRFRWWGLYTQRKPGFDGTWTGDENTDMLEDEYFMLRVRSDGGALTAKALRTLG
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
EISTEFARDTADISDRQNVQYHWIDVRNMPEIWRRLDEVGLQTTEACGDCPRVVLGSPLA
HHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCHHHHCCCCC
GESLDEVIDGTPAVNEIVKRYIGKPEYSNLPRKFKTAISGLQDVVHEVNDVAFIGVVHPE
CCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCC
HGPGFDLWVGGGLSTNPMLGQRVGAWVPLDEVPDVWEGVVSVFRDYGYRRLRSKARLKFL
CCCCCEEEECCCCCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDWGVEKFRQVLETEYLKRPLIDGPAPDPVTRPIDHVGVQKLKNGLNAVGVAPIAGRVS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
GTILSKVADLAEAAGSDRIRFTPYQKLIVLDVPDEKLEELRAGLDALGLPSTPSHWRRNL
HHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHHHHCCHHHCCCCCCHHHHHHHH
MACTGIEFCKLSFAETRSRSQVLVPELEKRLEDINAQLDVPVTININGCPNSCARIQVAD
HHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEE
IGFKGQMVDDGDGPQEGFQVHLGGSLGLDSGFGRKLRQHKVLATELGDYIERVVRNFVKQ
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REDGERFATWALRADEADLR
CCCCCHHHHEEEECCCCCCC
>Mature Secondary Structure 
TETPTRPAKPVKRPRGEGQWALGYREPLNANEQSKKDDNPLNVRERIENIYAPGGFDTI
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
DKGDLRGRFRWWGLYTQRKPGFDGTWTGDENTDMLEDEYFMLRVRSDGGALTAKALRTLG
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
EISTEFARDTADISDRQNVQYHWIDVRNMPEIWRRLDEVGLQTTEACGDCPRVVLGSPLA
HHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCHHHHCCCCC
GESLDEVIDGTPAVNEIVKRYIGKPEYSNLPRKFKTAISGLQDVVHEVNDVAFIGVVHPE
CCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCC
HGPGFDLWVGGGLSTNPMLGQRVGAWVPLDEVPDVWEGVVSVFRDYGYRRLRSKARLKFL
CCCCCEEEECCCCCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDWGVEKFRQVLETEYLKRPLIDGPAPDPVTRPIDHVGVQKLKNGLNAVGVAPIAGRVS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
GTILSKVADLAEAAGSDRIRFTPYQKLIVLDVPDEKLEELRAGLDALGLPSTPSHWRRNL
HHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHHHHCCHHHCCCCCCHHHHHHHH
MACTGIEFCKLSFAETRSRSQVLVPELEKRLEDINAQLDVPVTININGCPNSCARIQVAD
HHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEE
IGFKGQMVDDGDGPQEGFQVHLGGSLGLDSGFGRKLRQHKVLATELGDYIERVVRNFVKQ
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REDGERFATWALRADEADLR
CCCCCHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]