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The map label for this gene is dhbE [H]

Identifier: 119869566

GI number: 119869566

Start: 3721273

End: 3722922

Strand: Direct

Name: dhbE [H]

Synonym: Mkms_3534

Alternate gene names: 119869566

Gene position: 3721273-3722922 (Clockwise)

Preceding gene: 119869557

Following gene: 119869569

Centisome position: 64.86

GC content: 70.0

Gene sequence:

>1650_bases
ATGAGCACCGGATTCCAGCATGTGCACAGCGACCTGACCGATGGCTTCGTCCCATTCCCCGAAGACCGCGCCGAGCAGTA
CCGGCGGGCCGGGTACTGGACCGGGCGGCCCCTCGAGTCCCTTCTCCTTGACGCGGCACACCGACGGCCCGATCACCCGG
CCGTCGTCGACGTCGACGGATCCCTCACGTTCGCGGAGCTGACCGCGCGCGCCGACACCGTCGCCGCGGCGCTGGCCGGA
CTCGGGATCCGTCCTGGTGACCGGGTGCTGCTGCAACTACCCAACTCCGTGCGTTTCGCCGTCGCGTTCTTCGGTCTGCT
GCGGGCGGGCGCGGTGCCGGTGATGTGCCTGCCCGGCCACCGCACCGCCGAGCTGGGACACTTCGCCGACGTCAGCGGCG
CGGTGGCGCTGATCGTGCCCGACGAGGTCGGCGGCTTCGACTACCGCGAGATGGCCGCCCAGTTGGTGGCGGACCGGCCC
ACGCTGCGGCACGTGCTCGTCGACGGTGACCCGGGACCGTTCCTATCGTGGGCGGCACTGATCGACAGCGGCGGCGTGGC
ACCGGAGATCGGACCCGTCGACACCTCGCTGCCTGCCCTGCTGCTGGTCTCCGGCGGAACGACCGGACTGCCCAAGCTGA
TCCCCCGCACCCACGACGACTACGTCTACACCGCGGTGTCGAGCGCGCAGGCGTGCCACTTCACCCCCGACGACGTCTAC
CTGGTGGCGTTGCCGGCCGGCCACAACTTCCCGTTGGCGTGCCCCGGCATGCTGGGGGCAATGACCGTGGGGGCGACGAC
GGTGTTCACCGCCGATCCCAGCCCGGAGGAGGCGTTCGCGCTCATCGACAAACACCAGGTGACCGTCACCGGGCTGGTCA
ACGCGCTGGGCAAGCTGTGGGCGCAGGCGTGCGACTGGGAACCGGTGCTGCCGACGTCGCTGCGCCTGGTGCAGGTGGGC
GGTTCGCGGATGAGCCCGGAGGAGGCGCGGTTCATCCTCGATCGCCTGACCCCCGGGCTGTCCCAGATCTTCGGAATGGC
CGAGGGCATGCTGAATTTCACGCGGCCGGGAGATCCGCTCGACGTCGTGGTGCACACCCAGGGCAGGCCGGTCTCCCCGC
ACGACGAGATGCGGGTGGTCGACGAGTCCGGTGTCGAGGTCGCACCGGGTGAAGAGGGCGAACTGCTGGTGCGCGGTCCC
AACACACTCAACGGGTACTACCGGGCCGACGAGGCCAACGCCCGGTGCTTCAGCCCGGACGGCTTCTACCGCACCGGGGA
CCGGGTGCGGATCTTCGCCGACGGCCCGCTGGCCGGCAACGTCGAGGTGACCGGCCGGATCAAGGACGTCATCCACCGCG
GCGGTGAGACGGTTTCGGCGACCGACCTCGAGGACCATCTGCTGACCCACCCCGCGATCTATGCGGCGGCCGCCGTCGCG
CTACCCGACGACTATCTCGGTGAGAAGATCTGCGCCGCAGTGGTGTTCCGCGGCAAGCAGCTCACGCTCGCCGAACTCAA
CGCATTCCTCGACGAACGCGGGGCGTCCACTCACGCCCGGCCCGACGTGCTGGCTGCGATGCCGTCGCTGCCGCTGACCG
CGGTCGGCAAGGTCGACAAGAAGAAGCTCGTCGCCCAGCTGACGGGGTGA

Upstream 100 bases:

>100_bases
ACCGTGTTCCTCCAAGTCAACGTAGGCTGCCCTAACTTCAGGAAGGCTATCCTATATTCAGGGAGTCGAACACTCCTACC
TCGAGGGGCCTCTACGCGCG

Downstream 100 bases:

>100_bases
CCGCGCGTTACGAGCCGCGTTCGTGACCGGATGGCGGAAATCAGGGCCGGTTTCCGCCGTGACGTCACGAACGCGGACCG
TCGGCCCCCACTTATGCGGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSTGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDGSLTFAELTARADTVAAALAG
LGIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGHRTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRP
TLRHVLVDGDPGPFLSWAALIDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVY
LVALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLWAQACDWEPVLPTSLRLVQVG
GSRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPLDVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGP
NTLNGYYRADEANARCFSPDGFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVA
LPDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDKKKLVAQLTG

Sequences:

>Translated_549_residues
MSTGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDGSLTFAELTARADTVAAALAG
LGIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGHRTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRP
TLRHVLVDGDPGPFLSWAALIDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVY
LVALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLWAQACDWEPVLPTSLRLVQVG
GSRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPLDVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGP
NTLNGYYRADEANARCFSPDGFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVA
LPDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDKKKLVAQLTG
>Mature_548_residues
STGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDGSLTFAELTARADTVAAALAGL
GIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGHRTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRPT
LRHVLVDGDPGPFLSWAALIDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVYL
VALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLWAQACDWEPVLPTSLRLVQVGG
SRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPLDVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGPN
TLNGYYRADEANARCFSPDGFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVAL
PDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDKKKLVAQLTG

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=25.5434782608696, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=27.8388278388278, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=27.8388278388278, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=25.0943396226415, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI227499619, Length=572, Percent_Identity=25, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI157311624, Length=521, Percent_Identity=21.6890595009597, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157311622, Length=521, Percent_Identity=21.6890595009597, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=549, Percent_Identity=22.9508196721311, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=538, Percent_Identity=23.7918215613383, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=40, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI145693145, Length=553, Percent_Identity=30.7414104882459, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=27.5547445255474, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI221142682, Length=506, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790505, Length=523, Percent_Identity=25.8126195028681, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789201, Length=545, Percent_Identity=24.5871559633028, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=26.9303201506591, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=550, Percent_Identity=25.4545454545455, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=533, Percent_Identity=24.953095684803, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=519, Percent_Identity=24.4701348747592, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=519, Percent_Identity=24.4701348747592, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=519, Percent_Identity=24.4701348747592, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=23.3067729083665, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=503, Percent_Identity=25.2485089463221, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=529, Percent_Identity=22.8733459357278, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=550, Percent_Identity=24, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=540, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=540, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=374, Percent_Identity=24.8663101604278, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=497, Percent_Identity=23.943661971831, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=504, Percent_Identity=24.8015873015873, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24581924, Length=517, Percent_Identity=26.6924564796905, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=25.1953125, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=24.8565965583174, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24656500, Length=364, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550496, Length=546, Percent_Identity=23.992673992674, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552801, Length=546, Percent_Identity=23.992673992674, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648260, Length=364, Percent_Identity=25.2747252747253, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648253, Length=353, Percent_Identity=25.4957507082153, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648255, Length=353, Percent_Identity=25.4957507082153, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 58397; Mature: 58266

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDG
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECC
SLTFAELTARADTVAAALAGLGIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGH
CEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCC
RTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRPTLRHVLVDGDPGPFLSWAAL
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
IDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVY
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEEEECCCCEEECCCCCEE
LVALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLW
EEEEECCCCCCEECCCHHHHHHHCCEEEEECCCCHHHHHHHHHCCEEHHHHHHHHHHHHH
AQACDWEPVLPTSLRLVQVGGSRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPL
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCE
DVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGPNTLNGYYRADEANARCFSPD
EEEEECCCCCCCCCHHEEEECCCCCEECCCCCCCEEEECCCCCCCEEECCCCCCEEECCC
GFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVA
CCEECCCEEEEEECCCCCCCEEEEHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHC
LPDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDK
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHCCCCCH
KKLVAQLTG
HHHHHCCCC
>Mature Secondary Structure 
STGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDG
CCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECC
SLTFAELTARADTVAAALAGLGIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGH
CEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCC
RTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRPTLRHVLVDGDPGPFLSWAAL
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
IDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVY
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEEEECCCCEEECCCCCEE
LVALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLW
EEEEECCCCCCEECCCHHHHHHHCCEEEEECCCCHHHHHHHHHCCEEHHHHHHHHHHHHH
AQACDWEPVLPTSLRLVQVGGSRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPL
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCE
DVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGPNTLNGYYRADEANARCFSPD
EEEEECCCCCCCCCHHEEEECCCCCEECCCCCCCEEEECCCCCCCEEECCCCCCEEECCC
GFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVA
CCEECCCEEEEEECCCCCCCEEEEHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHC
LPDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDK
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHCCCCCH
KKLVAQLTG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]