Click here to switch to the map view.

The map label for this gene is dinB2 [H]

Identifier: 119867898

GI number: 119867898

Start: 1973751

End: 1974818

Strand: Reverse

Name: dinB2 [H]

Synonym: Mkms_1859

Alternate gene names: 119867898

Gene position: 1974818-1973751 (Counterclockwise)

Preceding gene: 119867899

Following gene: 119867897

Centisome position: 34.42

GC content: 70.41

Gene sequence:

>1068_bases
GTGCCGCGCTGGATCCTGCACGTCGACCTCGACCAGTTCCTCGCCTCGGTCGAGCTGCGCCGACACCCGGAACTGGCGGG
GCTGCCGGTGATCGTCGGTGGCAGCGGTGACCCGACCGAACCGCGAAAGGTGGTCACCTGCGCCTCCTACCGCGCGCGCG
AATTCGGGGTGCATGCCGGGATGCCCCTGCGCGTGGCGGCGCGGCGATGCCCGGATGCGACGTTCCTGCCGTCGGACACC
GCGGCCTACGACGAGGCCTCCGAACAGGTGATGGGGTTGTTGCGCGATCTGGGTCACCCGGTCGAGGTGTGGGGCTGGGA
CGAGGCCTACCTCGGCGCCGATGTCGAGGATCCGTTCGCGCTGGCCGAGCGCATCCGCGAAGTGGTCGCCGGTACCGGTC
TGTCGTGCTCGGTGGGCATCAGCGACAACAAGCAGCGCGCCAAGGTCGCCACCGGCTTCGGCAAGCCCGCCGGGGTGTTC
ATGCTCACCGACGCCAACTGGATGGCCGTGATGGGCGACCGTCCGGTCGACGCGCTCTGGGGGGTCGGGCCCAAGACGGC
GAAGAAGCTCACCGCCCTCGGCATCACCACGGTGGCCGATCTCGCCGCCACCGATGCCGCGGTGCTGACATCGACGTTCG
GACCGACGACCGGTCTGTGGATCCTGTTGCTGGCCAAGGGCGGTGGCGACGACACGGTCAGCGCAGCGCCGTGGGTGCCG
CGCTCACGCAGCCATGTGGTCACCTTCCCGCACGATCTCACCGACCGCGCGGAGATGACCGCGGCGGTGACGCGCCTGGC
CGAGCAGACGTTGACCGAGGTCGTCGAGGCCGGCCGCGTGGTCACCCGCGTGGCGGTGACCGTACGCACGAGCACCTTCT
ACACCCGCACGAAGATCCGTAAGCTGAGCGCCCCGACCACCGAGGCCGGCGTCGTCACCGCCACCGCCCTCGACGTGCTC
GCGCTGTTCGACCTCGACCGGCCGATCCGGCTCCTCGGGGTGCGTTTGGAGTTGGCGATGCCGGAAGCCCCCAAGCCCCC
GGAACCCCTTGTCGCCGCGGACCAATAG

Upstream 100 bases:

>100_bases
CGCCGACTACATCGAGCACCGTCTCGCCGACGGCGCCACGCTCGACGAGGTGGCCGACGCGTGGGAGGTCGTGGCCGTCA
AGCTGTGTGGGCGGTGAGGC

Downstream 100 bases:

>100_bases
CCTCGCCTCATGCTCCAGACTGTGGCGATCCGCGGTTACCGCTCACTGCGCGATGTGGTGCTCCCGCTGCGCCGGCTGAG
CGTGGTCACGGGAGCCAACG

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2 [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPRWILHVDLDQFLASVELRRHPELAGLPVIVGGSGDPTEPRKVVTCASYRAREFGVHAGMPLRVAARRCPDATFLPSDT
AAYDEASEQVMGLLRDLGHPVEVWGWDEAYLGADVEDPFALAERIREVVAGTGLSCSVGISDNKQRAKVATGFGKPAGVF
MLTDANWMAVMGDRPVDALWGVGPKTAKKLTALGITTVADLAATDAAVLTSTFGPTTGLWILLLAKGGGDDTVSAAPWVP
RSRSHVVTFPHDLTDRAEMTAAVTRLAEQTLTEVVEAGRVVTRVAVTVRTSTFYTRTKIRKLSAPTTEAGVVTATALDVL
ALFDLDRPIRLLGVRLELAMPEAPKPPEPLVAADQ

Sequences:

>Translated_355_residues
MPRWILHVDLDQFLASVELRRHPELAGLPVIVGGSGDPTEPRKVVTCASYRAREFGVHAGMPLRVAARRCPDATFLPSDT
AAYDEASEQVMGLLRDLGHPVEVWGWDEAYLGADVEDPFALAERIREVVAGTGLSCSVGISDNKQRAKVATGFGKPAGVF
MLTDANWMAVMGDRPVDALWGVGPKTAKKLTALGITTVADLAATDAAVLTSTFGPTTGLWILLLAKGGGDDTVSAAPWVP
RSRSHVVTFPHDLTDRAEMTAAVTRLAEQTLTEVVEAGRVVTRVAVTVRTSTFYTRTKIRKLSAPTTEAGVVTATALDVL
ALFDLDRPIRLLGVRLELAMPEAPKPPEPLVAADQ
>Mature_354_residues
PRWILHVDLDQFLASVELRRHPELAGLPVIVGGSGDPTEPRKVVTCASYRAREFGVHAGMPLRVAARRCPDATFLPSDTA
AYDEASEQVMGLLRDLGHPVEVWGWDEAYLGADVEDPFALAERIREVVAGTGLSCSVGISDNKQRAKVATGFGKPAGVFM
LTDANWMAVMGDRPVDALWGVGPKTAKKLTALGITTVADLAATDAAVLTSTFGPTTGLWILLLAKGGGDDTVSAAPWVPR
SRSHVVTFPHDLTDRAEMTAAVTRLAEQTLTEVVEAGRVVTRVAVTVRTSTFYTRTKIRKLSAPTTEAGVVTATALDVLA
LFDLDRPIRLLGVRLELAMPEAPKPPEPLVAADQ

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=361, Percent_Identity=26.5927977839335, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI7706681, Length=362, Percent_Identity=26.5193370165746, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI7705344, Length=108, Percent_Identity=36.1111111111111, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786425, Length=354, Percent_Identity=30.7909604519774, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787432, Length=324, Percent_Identity=27.4691358024691, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537959, Length=344, Percent_Identity=29.3604651162791, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193205700, Length=423, Percent_Identity=24.822695035461, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19923006, Length=407, Percent_Identity=26.2899262899263, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37966; Mature: 37835

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRWILHVDLDQFLASVELRRHPELAGLPVIVGGSGDPTEPRKVVTCASYRAREFGVHAG
CCCEEEEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHCCCCC
MPLRVAARRCPDATFLPSDTAAYDEASEQVMGLLRDLGHPVEVWGWDEAYLGADVEDPFA
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCHHH
LAERIREVVAGTGLSCSVGISDNKQRAKVATGFGKPAGVFMLTDANWMAVMGDRPVDALW
HHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCHHHHH
GVGPKTAKKLTALGITTVADLAATDAAVLTSTFGPTTGLWILLLAKGGGDDTVSAAPWVP
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
RSRSHVVTFPHDLTDRAEMTAAVTRLAEQTLTEVVEAGRVVTRVAVTVRTSTFYTRTKIR
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCEEHHHHHH
KLSAPTTEAGVVTATALDVLALFDLDRPIRLLGVRLELAMPEAPKPPEPLVAADQ
HCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRWILHVDLDQFLASVELRRHPELAGLPVIVGGSGDPTEPRKVVTCASYRAREFGVHAG
CCEEEEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHCCCCC
MPLRVAARRCPDATFLPSDTAAYDEASEQVMGLLRDLGHPVEVWGWDEAYLGADVEDPFA
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCHHH
LAERIREVVAGTGLSCSVGISDNKQRAKVATGFGKPAGVFMLTDANWMAVMGDRPVDALW
HHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCHHHHH
GVGPKTAKKLTALGITTVADLAATDAAVLTSTFGPTTGLWILLLAKGGGDDTVSAAPWVP
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
RSRSHVVTFPHDLTDRAEMTAAVTRLAEQTLTEVVEAGRVVTRVAVTVRTSTFYTRTKIR
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCEEHHHHHH
KLSAPTTEAGVVTATALDVLALFDLDRPIRLLGVRLELAMPEAPKPPEPLVAADQ
HCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]