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The map label for this gene is ykfB [H]

Identifier: 119867443

GI number: 119867443

Start: 1496973

End: 1498076

Strand: Direct

Name: ykfB [H]

Synonym: Mkms_1393

Alternate gene names: 119867443

Gene position: 1496973-1498076 (Clockwise)

Preceding gene: 119867442

Following gene: 119867444

Centisome position: 26.09

GC content: 69.29

Gene sequence:

>1104_bases
GTGAAGATCACTCGGGTCGACGCGATCCCGTTCGCCATTCCCTACACCAAACCCCTGAAGTTCGCCTCGGGTGAGGTCAC
CGACGCCGAACACGTTCTGGTGCGCGTGCACACCGACGACGGGGTCGTCGGCGTCGCCGAGGCCCCACCGCGGCCGTTCA
CCTACGGCGAGACCCAGGCGGGCATCGTCGCGGTCATCGAGCGGATCTTCGCGCCGCAGGTGGTCGGGCTCACCCTGACC
GAACGCGAGGTCGTCGGCGCGCGCCTGAGCCGCACCGTCGGCAACCCCACCGCCAAATCCGCGGTGGACATGGCGATCTG
GGATGCCCTGGGCCGCACCCTCGGGCTTCCGGTCAGCGATCTGCTCGGCGGGTTCACCGACCGTATGCGCGTGAGCCACA
TGCTCGGATTCGACAGCCCGGCAGCGATGGTGGCCGAAGCCGAGCGCATGCGCGACACATTCGGCATCCGCACCTTCAAG
GTCAAGGTCGGCCGCCGTCCGGTCTCGCTCGACACCGCCGTCGTGCGGGCGCTGCGTGAGCACTTCGGCGACGGCGTCGA
ACTCTACGTCGACGGTAACCGCGGCTGGAGCGCGTCGGAGTCCCTACGGGCGATGACGCAGATGGCCGACCTCGACCTGC
TGTTCGCCGAGGAGTTGTGTCCCGCCGACGACGTCCTCGGCCGGCGGCGCCTCGTCGGACAGATCGACATACCGTTCATC
GCCGACGAATCCGTGCCGACCCCCGCCGACGTGACCCGCGAACTGCTCGGCGGGTCGGCCACCGCGATCAGCATCAAGAC
CGCGCGCACCGGCTTCACCCAGTCCCAGCGGGTGCACCACCTCGCCGAGGGACTCGGCGTCGAGATGGTGATCGGCAACC
AGATCGACGGCCAGATCGGCACGGCCTGCGCGGTCGCCTTCGGCTCCGCGTTCGCCGCCACCACCCGCCACGCCGGTGAG
CTGTCGAACTTCCTCGACATGAGCGACGACCTACTCACCGAGGGACTGCACATTCGCGACGGCGTCCTGCGCCGTCCCGC
GGGCAACGGCCTGGGCATCGAGGTCGACCCGGACAAACTCGCCCGCTACCGCACCGACCACTGA

Upstream 100 bases:

>100_bases
GCGGCGAGGACGACCCTCTGGTCGAGGCCGTGGTCGGCGTGCTCGCCGCCGCCTTCTCCGTCCACGCGATCGCCGATTCG
ATGCCAACCGGAAGGCCGCT

Downstream 100 bases:

>100_bases
CATCAGCAACCACCACCAAGGAGTACGACGATGACCACCTTCGACACCCCGGTTCCCACCGCCACCGCCGCGGCGTCCGG
CGCCTCGGCCACCGACCGCT

Product: mandelate racemase/muconate lactonizing protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKITRVDAIPFAIPYTKPLKFASGEVTDAEHVLVRVHTDDGVVGVAEAPPRPFTYGETQAGIVAVIERIFAPQVVGLTLT
EREVVGARLSRTVGNPTAKSAVDMAIWDALGRTLGLPVSDLLGGFTDRMRVSHMLGFDSPAAMVAEAERMRDTFGIRTFK
VKVGRRPVSLDTAVVRALREHFGDGVELYVDGNRGWSASESLRAMTQMADLDLLFAEELCPADDVLGRRRLVGQIDIPFI
ADESVPTPADVTRELLGGSATAISIKTARTGFTQSQRVHHLAEGLGVEMVIGNQIDGQIGTACAVAFGSAFAATTRHAGE
LSNFLDMSDDLLTEGLHIRDGVLRRPAGNGLGIEVDPDKLARYRTDH

Sequences:

>Translated_367_residues
MKITRVDAIPFAIPYTKPLKFASGEVTDAEHVLVRVHTDDGVVGVAEAPPRPFTYGETQAGIVAVIERIFAPQVVGLTLT
EREVVGARLSRTVGNPTAKSAVDMAIWDALGRTLGLPVSDLLGGFTDRMRVSHMLGFDSPAAMVAEAERMRDTFGIRTFK
VKVGRRPVSLDTAVVRALREHFGDGVELYVDGNRGWSASESLRAMTQMADLDLLFAEELCPADDVLGRRRLVGQIDIPFI
ADESVPTPADVTRELLGGSATAISIKTARTGFTQSQRVHHLAEGLGVEMVIGNQIDGQIGTACAVAFGSAFAATTRHAGE
LSNFLDMSDDLLTEGLHIRDGVLRRPAGNGLGIEVDPDKLARYRTDH
>Mature_367_residues
MKITRVDAIPFAIPYTKPLKFASGEVTDAEHVLVRVHTDDGVVGVAEAPPRPFTYGETQAGIVAVIERIFAPQVVGLTLT
EREVVGARLSRTVGNPTAKSAVDMAIWDALGRTLGLPVSDLLGGFTDRMRVSHMLGFDSPAAMVAEAERMRDTFGIRTFK
VKVGRRPVSLDTAVVRALREHFGDGVELYVDGNRGWSASESLRAMTQMADLDLLFAEELCPADDVLGRRRLVGQIDIPFI
ADESVPTPADVTRELLGGSATAISIKTARTGFTQSQRVHHLAEGLGVEMVIGNQIDGQIGTACAVAFGSAFAATTRHAGE
LSNFLDMSDDLLTEGLHIRDGVLRRPAGNGLGIEVDPDKLARYRTDH

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=360, Percent_Identity=24.1666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081876, Length=292, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 39499; Mature: 39499

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRVDAIPFAIPYTKPLKFASGEVTDAEHVLVRVHTDDGVVGVAEAPPRPFTYGETQA
CCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCH
GIVAVIERIFAPQVVGLTLTEREVVGARLSRTVGNPTAKSAVDMAIWDALGRTLGLPVSD
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
LLGGFTDRMRVSHMLGFDSPAAMVAEAERMRDTFGIRTFKVKVGRRPVSLDTAVVRALRE
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
HFGDGVELYVDGNRGWSASESLRAMTQMADLDLLFAEELCPADDVLGRRRLVGQIDIPFI
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
ADESVPTPADVTRELLGGSATAISIKTARTGFTQSQRVHHLAEGLGVEMVIGNQIDGQIG
ECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHH
TACAVAFGSAFAATTRHAGELSNFLDMSDDLLTEGLHIRDGVLRRPAGNGLGIEVDPDKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCCCCCCCEEECHHHH
ARYRTDH
HHCCCCC
>Mature Secondary Structure
MKITRVDAIPFAIPYTKPLKFASGEVTDAEHVLVRVHTDDGVVGVAEAPPRPFTYGETQA
CCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCH
GIVAVIERIFAPQVVGLTLTEREVVGARLSRTVGNPTAKSAVDMAIWDALGRTLGLPVSD
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
LLGGFTDRMRVSHMLGFDSPAAMVAEAERMRDTFGIRTFKVKVGRRPVSLDTAVVRALRE
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
HFGDGVELYVDGNRGWSASESLRAMTQMADLDLLFAEELCPADDVLGRRRLVGQIDIPFI
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
ADESVPTPADVTRELLGGSATAISIKTARTGFTQSQRVHHLAEGLGVEMVIGNQIDGQIG
ECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHH
TACAVAFGSAFAATTRHAGELSNFLDMSDDLLTEGLHIRDGVLRRPAGNGLGIEVDPDKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCCCCCCCEEECHHHH
ARYRTDH
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]