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The map label for this gene is sseA [H]

Identifier: 119867358

GI number: 119867358

Start: 1400238

End: 1401134

Strand: Reverse

Name: sseA [H]

Synonym: Mkms_1308

Alternate gene names: 119867358

Gene position: 1401134-1400238 (Counterclockwise)

Preceding gene: 119867359

Following gene: 119867357

Centisome position: 24.42

GC content: 68.0

Gene sequence:

>897_bases
GTGCCGCTGCCACCTGATCCCAGCCCGAAACTCGCCGACTACGCCCATCCGGAACGCCTCGTCACCAGCGACTGGCTGGC
CGGCAATCTCGGCCGCCCCGGCCTGGCCATCGTCGAGTCCGATGAGGACGTCCTGCTCTACGACACCGGCCACATCCCCG
GCGCGGTGAAGATCGACTGGCATGTCGACCTCAACGATCCGAACGTCCGCGACTACATCAACGGTGAGCAGTTCGCCGCG
TTGATGGACCGCAAGGGAATCAGCCGCGACGACACCGTGGTGATCTACGGCGACAAGAGCAACTGGTGGGCGGCCTACGC
GCTGTGGGTCTTCACCTTGTTCGGGCATCCCGACGTCCGCCTGCTCGACGGCGGACGCGATCTGTGGATCTCCGACGGCC
GCGACACCACCCTCGACGTACCGTCCAGGCAGACCAGCGGCTACCCCGTGGTCGAACGCGACGACGCCCCGATCCGCGCC
TTCCGCGAGGACGTCCTGGGGATCCTGGGCAAGCAGCCGCTGATCGACGTCCGCTCGCCCGCCGAGTACACCGGTGAACG
CACCCACATGCCCGACTATCCGGAGGAGGGCGCGTTGCGTGGCGGGCACATCCCGACCGCGCGGTCGATCCCATGGAGTA
AGGCGGCCCGGGACAACGGGCAGTTCCGCACCCGCGCCGAACTCGAGGAGCTCTACGGCTTCCTCGAGCCCGACGACGAA
ACCGTCGTCTACTGCCGGATCGGGGAACGCTCGAGCCACACCTGGTTCGTCCTGACCCACCTGCTCGGGCTGCCGGGTGT
GCGCAACTACGACGGCTCCTGGACCGAATGGGGTAACGCGGTGCGTGTCCCCGTCGCGGTCGGTCCCGATCCGGGCGAGG
CACCCGCGTCGTCATGA

Upstream 100 bases:

>100_bases
GTGCGCCGCATGCTGGACCGGCTCGACCTGTCGGTCCCGGCGCTCTGGACGCGCTGACCAGGGACCCGCCGCACCCGCGT
CCGGCGGTAGGCTCGTTTCC

Downstream 100 bases:

>100_bases
CCATGCCGGCCCCTCTCGCCGAGGTCGTCTCCGAGTTCCAGGAGGTGCAGGGACAGGACAAGCTACGGCTGCTCCTCGAG
TTCGCCGACGACCTGCCCGC

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPLPPDPSPKLADYAHPERLVTSDWLAGNLGRPGLAIVESDEDVLLYDTGHIPGAVKIDWHVDLNDPNVRDYINGEQFAA
LMDRKGISRDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLDGGRDLWISDGRDTTLDVPSRQTSGYPVVERDDAPIRA
FREDVLGILGKQPLIDVRSPAEYTGERTHMPDYPEEGALRGGHIPTARSIPWSKAARDNGQFRTRAELEELYGFLEPDDE
TVVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTEWGNAVRVPVAVGPDPGEAPASS

Sequences:

>Translated_298_residues
MPLPPDPSPKLADYAHPERLVTSDWLAGNLGRPGLAIVESDEDVLLYDTGHIPGAVKIDWHVDLNDPNVRDYINGEQFAA
LMDRKGISRDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLDGGRDLWISDGRDTTLDVPSRQTSGYPVVERDDAPIRA
FREDVLGILGKQPLIDVRSPAEYTGERTHMPDYPEEGALRGGHIPTARSIPWSKAARDNGQFRTRAELEELYGFLEPDDE
TVVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTEWGNAVRVPVAVGPDPGEAPASS
>Mature_297_residues
PLPPDPSPKLADYAHPERLVTSDWLAGNLGRPGLAIVESDEDVLLYDTGHIPGAVKIDWHVDLNDPNVRDYINGEQFAAL
MDRKGISRDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLDGGRDLWISDGRDTTLDVPSRQTSGYPVVERDDAPIRAF
REDVLGILGKQPLIDVRSPAEYTGERTHMPDYPEEGALRGGHIPTARSIPWSKAARDNGQFRTRAELEELYGFLEPDDET
VVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTEWGNAVRVPVAVGPDPGEAPASS

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=277, Percent_Identity=28.5198555956679, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI194473668, Length=296, Percent_Identity=29.0540540540541, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI61835204, Length=273, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194473681, Length=273, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87082121, Length=282, Percent_Identity=28.0141843971631, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081967, Length=304, Percent_Identity=26.9736842105263, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=249, Percent_Identity=28.9156626506024, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33168; Mature: 33037

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPPDPSPKLADYAHPERLVTSDWLAGNLGRPGLAIVESDEDVLLYDTGHIPGAVKIDW
CCCCCCCCCCCCCCCCCCHHECCCHHCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEE
HVDLNDPNVRDYINGEQFAALMDRKGISRDDTVVIYGDKSNWWAAYALWVFTLFGHPDVR
EEECCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEE
LLDGGRDLWISDGRDTTLDVPSRQTSGYPVVERDDAPIRAFREDVLGILGKQPLIDVRSP
EEECCCEEEEECCCCCEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
AEYTGERTHMPDYPEEGALRGGHIPTARSIPWSKAARDNGQFRTRAELEELYGFLEPDDE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHHCCCCCCCC
TVVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTEWGNAVRVPVAVGPDPGEAPASS
EEEEEEECCCCCCCEEHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PLPPDPSPKLADYAHPERLVTSDWLAGNLGRPGLAIVESDEDVLLYDTGHIPGAVKIDW
CCCCCCCCCCCCCCCCCHHECCCHHCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEE
HVDLNDPNVRDYINGEQFAALMDRKGISRDDTVVIYGDKSNWWAAYALWVFTLFGHPDVR
EEECCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEE
LLDGGRDLWISDGRDTTLDVPSRQTSGYPVVERDDAPIRAFREDVLGILGKQPLIDVRSP
EEECCCEEEEECCCCCEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
AEYTGERTHMPDYPEEGALRGGHIPTARSIPWSKAARDNGQFRTRAELEELYGFLEPDDE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHHCCCCCCCC
TVVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTEWGNAVRVPVAVGPDPGEAPASS
EEEEEEECCCCCCCEEHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]