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The map label for this gene is glnG [H]

Identifier: 119776559

GI number: 119776559

Start: 4057060

End: 4058472

Strand: Direct

Name: glnG [H]

Synonym: Sama_3427

Alternate gene names: 119776559

Gene position: 4057060-4058472 (Clockwise)

Preceding gene: 119776558

Following gene: 119776564

Centisome position: 94.22

GC content: 60.08

Gene sequence:

>1413_bases
ATGAGTATCAGTGAACAGGTTTGGATCCTCGACGACGACAGCTCTATCCGCTGGGTGCTGGAAAAAGCCCTCAAGAGCGC
CCGCATCACCAGCGCCAGCTTCGCCGCAGCAGAGTCTCTGTGGCAGGCATTGGAGCTGAGCCAGCCGCTGGTGATAGTGT
CGGACATCCGCATGCCCGGCACCGATGGCTTAACGCTGCTGGAGCGTATTGGCCTGCATTACCCCCATATTCCGGTCATC
ATAATGACGGCGCACTCGGACCTCGACAGCGCCGTGGCCGCTTACCAGTCCGGCGCCTTTGAATACTTACCCAAGCCCTT
CGACATCGACGAAGCCATCGCCCTGGTGGAGCGGGCACTGACCCATGCCAGTGAACAGGCGCCCCAGGCCAATACCAGTG
AACCGGTCAAGGCACCGGAAATCATCGGTGAGGCCCCTGCCATGCAGGAGGTGTTTCGCGCTATCGGCCGTCTGTCACGC
TCATCCATCAGCGTACTGATCAACGGCCAATCCGGTACGGGCAAGGAATTGGTGGCAGGGGCGCTGCACAAACACAGTCC
CCGCAAGGACAAGCCCTTTATCGCGCTGAACATGGCAGCCATTCCCAAGGACCTGATTGAATCTGAGCTGTTTGGCCACG
AGAAAGGTGCCTTTACCGGCGCTGCCGGTGTGCGTCAGGGCCGCTTCGAGCAGGCCAACGGTGGCACCCTGTTTTTGGAC
GAGATCGGCGACATGCCCCTGGATGTGCAAACCCGGCTGCTGCGGGTGCTGGCCGATGGTCAGTTTTACCGTGTTGGTGG
CCACTCACCGGTGCAGGTGGACGTACGCATTATTGCCGCCACCCACCAGGATCTGGAGCGGTTGGTGCAACAGGGGCAAT
TCCGGGAAGACCTGTTTCACCGCCTCAATGTGATCAGGGTGCATCTGCCACCACTCAGGGAGCGCCGTGAAGATATTCCG
CAACTGACCCGCCACTTTCTGGCCGCCGCCGCCCGGGAGATTGGCGTTGAAGCCAAGATACTTGCCAAAGAAACCGCCGC
CAAACTGCAGCAGCTACCCTGGCCCGGCAACGTGCGCCAGTTGGAGAATACCTGCCGCTGGCTCACAGTAATGGCATCGG
GGCAGGAGATACTGCCACAGGACCTGCCACCGGAGCTTTTTAAAGATCCGGTCGTCCATCATGCCGGCGCCGTGGTGGCC
GGAGACTGGGAGAGTGCCCTGAGGGATATAGTCGATAGACGCCTCGGTGAAGGGGAGTCGGATCTGCTGGCTGACATGCA
GCCCACCTTTGAGCGCATCATGCTCGAAGTCGCGCTCAAACATACCCAGGGCCATAAACAGGAAGCCGCACGACGTCTCG
GCTGGGGCCGTAACACCCTTACCCGCAAACTCAAAGAGCTTGAGATGGATTAG

Upstream 100 bases:

>100_bases
TCATGGTGGCCGCATCGACTGCGACTCAGCCCCCGGTCACACCCAGTTTGTGCTCACCCTGCCCATTAAAACCGGAAAAT
AGGACCCAAAAGGAGACTGC

Downstream 100 bases:

>100_bases
CACCTTTTGAGACACAAAGAAAAGGGACGCAGCATGCGTCCCTTTTCGTTAAACAATCGGCTGACTCAGAAGCGCCAGTG
CAGGCCCACGCCATAATGGT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSISEQVWILDDDSSIRWVLEKALKSARITSASFAAAESLWQALELSQPLVIVSDIRMPGTDGLTLLERIGLHYPHIPVI
IMTAHSDLDSAVAAYQSGAFEYLPKPFDIDEAIALVERALTHASEQAPQANTSEPVKAPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAAGVRQGRFEQANGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLERLVQQGQFREDLFHRLNVIRVHLPPLRERREDIP
QLTRHFLAAAAREIGVEAKILAKETAAKLQQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELFKDPVVHHAGAVVA
GDWESALRDIVDRRLGEGESDLLADMQPTFERIMLEVALKHTQGHKQEAARRLGWGRNTLTRKLKELEMD

Sequences:

>Translated_470_residues
MSISEQVWILDDDSSIRWVLEKALKSARITSASFAAAESLWQALELSQPLVIVSDIRMPGTDGLTLLERIGLHYPHIPVI
IMTAHSDLDSAVAAYQSGAFEYLPKPFDIDEAIALVERALTHASEQAPQANTSEPVKAPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAAGVRQGRFEQANGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLERLVQQGQFREDLFHRLNVIRVHLPPLRERREDIP
QLTRHFLAAAAREIGVEAKILAKETAAKLQQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELFKDPVVHHAGAVVA
GDWESALRDIVDRRLGEGESDLLADMQPTFERIMLEVALKHTQGHKQEAARRLGWGRNTLTRKLKELEMD
>Mature_469_residues
SISEQVWILDDDSSIRWVLEKALKSARITSASFAAAESLWQALELSQPLVIVSDIRMPGTDGLTLLERIGLHYPHIPVII
MTAHSDLDSAVAAYQSGAFEYLPKPFDIDEAIALVERALTHASEQAPQANTSEPVKAPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAAGVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLERLVQQGQFREDLFHRLNVIRVHLPPLRERREDIPQ
LTRHFLAAAAREIGVEAKILAKETAAKLQQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELFKDPVVHHAGAVVAG
DWESALRDIVDRRLGEGESDLLADMQPTFERIMLEVALKHTQGHKQEAARRLGWGRNTLTRKLKELEMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=71.1206896551724, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=474, Percent_Identity=40.7172995780591, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=40.9871244635193, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1788905, Length=423, Percent_Identity=39.9527186761229, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=43.2748538011696, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789087, Length=365, Percent_Identity=40.8219178082192, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=49.1596638655462, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786524, Length=372, Percent_Identity=40.3225806451613, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=41.6413373860182, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87082152, Length=253, Percent_Identity=46.2450592885375, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=225, Percent_Identity=48, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=360, Percent_Identity=31.3888888888889, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=38.4279475982533, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=28.3582089552239, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52081; Mature: 51950

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISEQVWILDDDSSIRWVLEKALKSARITSASFAAAESLWQALELSQPLVIVSDIRMPG
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TDGLTLLERIGLHYPHIPVIIMTAHSDLDSAVAAYQSGAFEYLPKPFDIDEAIALVERAL
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
THASEQAPQANTSEPVKAPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAG
HHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
ALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAAGVRQGRFEQANGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
EIGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLERLVQQGQFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHHHHH
RLNVIRVHLPPLRERREDIPQLTRHFLAAAAREIGVEAKILAKETAAKLQQLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
LENTCRWLTVMASGQEILPQDLPPELFKDPVVHHAGAVVAGDWESALRDIVDRRLGEGES
HHHHHHEEEEEECCCHHCCCCCCHHHHHCCHHHHCCCEEECCHHHHHHHHHHHHCCCCHH
DLLADMQPTFERIMLEVALKHTQGHKQEAARRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SISEQVWILDDDSSIRWVLEKALKSARITSASFAAAESLWQALELSQPLVIVSDIRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TDGLTLLERIGLHYPHIPVIIMTAHSDLDSAVAAYQSGAFEYLPKPFDIDEAIALVERAL
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
THASEQAPQANTSEPVKAPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAG
HHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
ALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAAGVRQGRFEQANGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
EIGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLERLVQQGQFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHHHHH
RLNVIRVHLPPLRERREDIPQLTRHFLAAAAREIGVEAKILAKETAAKLQQLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
LENTCRWLTVMASGQEILPQDLPPELFKDPVVHHAGAVVAGDWESALRDIVDRRLGEGES
HHHHHHEEEEEECCCHHCCCCCCHHHHHCCHHHHCCCEEECCHHHHHHHHHHHHCCCCHH
DLLADMQPTFERIMLEVALKHTQGHKQEAARRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]