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The map label for this gene is ntrB [H]

Identifier: 119776558

GI number: 119776558

Start: 4055995

End: 4057041

Strand: Direct

Name: ntrB [H]

Synonym: Sama_3426

Alternate gene names: 119776558

Gene position: 4055995-4057041 (Clockwise)

Preceding gene: 119776557

Following gene: 119776559

Centisome position: 94.19

GC content: 57.88

Gene sequence:

>1047_bases
ATGGATACTCAAAACCTGCTCAATCACCTGGTGACCGCTGTACTGGTCATAGACTCAGGTCTAAAACCCTGCTTTGCCAA
TGCTGCTGCCGAGCAGTTGCTTGGCACCGGCAGTCATCGCCTCAGCGAACAAGCCCTGACCGATATTTTTCAGTTGCAGG
AAGTACCTGCCACCATACTGGCCGATGCGGTACGTCAGGGACTGGGGCTGACGGTCAACACCGCCAGCCTGGTCACCTTC
GATGGCCAACACCACACGGTGGATCTCACCCTGATCCCACTGGAAAATGAACCCGGCCTGAGTTTGCTTGAGCTGCGTCA
GGTGGACCAGCAGCGCCGTATCCACCAACAACTGACCCAGGATGCCCAGCAGCAGGCGGCACAGTTTCTGGTGCGTAACC
TCGCCCACGAGATAAAAAATCCCCTCGGTGGCCTGCGGGGCGCGGCGCAGTTACTGTCCCGGGAGCTGGAAGATGAATCA
CTCAAGGAATTCACCACCCTGATTATCGAACAGGCCGATCGCATGCGCGCCCTGGTGGATAAGCTGCTGGGGCCACAAAA
GCCCACGGCGCATCTGCCGCACAACATCCATGAAGTCGCTGAAAAAGTGATCAAGCTGGTTGAACTGTCCACGCCTGCCA
ATATCCGTGTCATCAGGGACTACGACCCTTCCATTCCCGACATAGATTTGGACGCCGACCAGATGCAGCAGGCGCTGCTC
AACATAGTGCAAAACGCTGTGCAGGCACTGGAAAACAGCGGAGGCACCATCACGGTGCGCACCCGCACCAGCCACCAGGT
CACCATAGGCGCCCAGCGTCACAAACTGGTGCTGATGTTGTCTGTTATTGATGATGGCCCGGGGATACCGCCGGAATTGA
TGGATACCCTCTTCTACCCCATGGTAACCGGCCGCGAACAGGGCTCTGGCCTGGGGCTGTCCATCGCCCACAACATCGCC
AGGCTTCATGGTGGCCGCATCGACTGCGACTCAGCCCCCGGTCACACCCAGTTTGTGCTCACCCTGCCCATTAAAACCGG
AAAATAG

Upstream 100 bases:

>100_bases
GTTAATCAAACCCAACTCACGCCAAAGGCCACTGCTGACACTTGCGGACTGCACCATATTGGTGCACCATATTGGTGCAG
ACCCAGTCAGGAAAGGCCCG

Downstream 100 bases:

>100_bases
GACCCAAAAGGAGACTGCATGAGTATCAGTGAACAGGTTTGGATCCTCGACGACGACAGCTCTATCCGCTGGGTGCTGGA
AAAAGCCCTCAAGAGCGCCC

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDTQNLLNHLVTAVLVIDSGLKPCFANAAAEQLLGTGSHRLSEQALTDIFQLQEVPATILADAVRQGLGLTVNTASLVTF
DGQHHTVDLTLIPLENEPGLSLLELRQVDQQRRIHQQLTQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELEDES
LKEFTTLIIEQADRMRALVDKLLGPQKPTAHLPHNIHEVAEKVIKLVELSTPANIRVIRDYDPSIPDIDLDADQMQQALL
NIVQNAVQALENSGGTITVRTRTSHQVTIGAQRHKLVLMLSVIDDGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHGGRIDCDSAPGHTQFVLTLPIKTGK

Sequences:

>Translated_348_residues
MDTQNLLNHLVTAVLVIDSGLKPCFANAAAEQLLGTGSHRLSEQALTDIFQLQEVPATILADAVRQGLGLTVNTASLVTF
DGQHHTVDLTLIPLENEPGLSLLELRQVDQQRRIHQQLTQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELEDES
LKEFTTLIIEQADRMRALVDKLLGPQKPTAHLPHNIHEVAEKVIKLVELSTPANIRVIRDYDPSIPDIDLDADQMQQALL
NIVQNAVQALENSGGTITVRTRTSHQVTIGAQRHKLVLMLSVIDDGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHGGRIDCDSAPGHTQFVLTLPIKTGK
>Mature_348_residues
MDTQNLLNHLVTAVLVIDSGLKPCFANAAAEQLLGTGSHRLSEQALTDIFQLQEVPATILADAVRQGLGLTVNTASLVTF
DGQHHTVDLTLIPLENEPGLSLLELRQVDQQRRIHQQLTQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELEDES
LKEFTTLIIEQADRMRALVDKLLGPQKPTAHLPHNIHEVAEKVIKLVELSTPANIRVIRDYDPSIPDIDLDADQMQQALL
NIVQNAVQALENSGGTITVRTRTSHQVTIGAQRHKLVLMLSVIDDGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHGGRIDCDSAPGHTQFVLTLPIKTGK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=344, Percent_Identity=50.5813953488372, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788549, Length=342, Percent_Identity=26.6081871345029, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=30.3149606299213, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786600, Length=339, Percent_Identity=26.5486725663717, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790861, Length=258, Percent_Identity=28.2945736434109, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081816, Length=250, Percent_Identity=25.6, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786912, Length=273, Percent_Identity=27.1062271062271, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38006; Mature: 38006

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQNLLNHLVTAVLVIDSGLKPCFANAAAEQLLGTGSHRLSEQALTDIFQLQEVPATIL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ADAVRQGLGLTVNTASLVTFDGQHHTVDLTLIPLENEPGLSLLELRQVDQQRRIHQQLTQ
HHHHHHCCCEEEECCEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELEDESLKEFTTLIIEQADRMRALVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KLLGPQKPTAHLPHNIHEVAEKVIKLVELSTPANIRVIRDYDPSIPDIDLDADQMQQALL
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
NIVQNAVQALENSGGTITVRTRTSHQVTIGAQRHKLVLMLSVIDDGPGIPPELMDTLFYP
HHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNIARLHGGRIDCDSAPGHTQFVLTLPIKTGK
HHCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MDTQNLLNHLVTAVLVIDSGLKPCFANAAAEQLLGTGSHRLSEQALTDIFQLQEVPATIL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ADAVRQGLGLTVNTASLVTFDGQHHTVDLTLIPLENEPGLSLLELRQVDQQRRIHQQLTQ
HHHHHHCCCEEEECCEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELEDESLKEFTTLIIEQADRMRALVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KLLGPQKPTAHLPHNIHEVAEKVIKLVELSTPANIRVIRDYDPSIPDIDLDADQMQQALL
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
NIVQNAVQALENSGGTITVRTRTSHQVTIGAQRHKLVLMLSVIDDGPGIPPELMDTLFYP
HHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNIARLHGGRIDCDSAPGHTQFVLTLPIKTGK
HHCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]