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The map label for this gene is rep [H]

Identifier: 119776398

GI number: 119776398

Start: 3877655

End: 3879667

Strand: Reverse

Name: rep [H]

Synonym: Sama_3266

Alternate gene names: 119776398

Gene position: 3879667-3877655 (Counterclockwise)

Preceding gene: 119776404

Following gene: 119776385

Centisome position: 90.1

GC content: 54.5

Gene sequence:

>2013_bases
ATGAAGCTGAATCCCGCGCAAAATGATGCAGTCCATTATGTTTCCGGCCCCTGTCTGGTGCTGGCGGGTGCGGGCAGCGG
TAAAACGCGGGTGATCATCAACAAAATTGCCTATCTGGTGCAGAAGTGCGGCTATCAGGCTCGCAATATTGCCGCCGTGA
CCTTCACCAACAAGGCGGCGCGGGAGATGAAAGAGCGGGTAGCCCAATCCATGGGCCGCAAAGAGGCGCGGGGGCTGTGG
ATTTCTACCTTCCACACTCTGGGGCTGGAAATTCTCAAGCGTGAACACAAGGCTGTAGGGCTGAAGGCCGGGTTTTCGCT
GTTTGATGATCAGGACACCCTGGCGCTTTTAAAGGAACTGACCGAAAAAGAGCTTCAGGAAGACAAGGATCTGCTCAAGC
TGCTGGCGACCTCAATTTCCAACTGGAAGGGGGCGCTGGTGATCCCGGATCATGCTCGCAAGATAGCCAAAGATGAGCGG
GAGCAGTTGTTTGCGCTCCTGTACCAGCGTTATGCCCAGCACATGAAGGCATACAATGCCCTCGATTTTGATGACCTTAT
TCTGCTGCCGACATTGCTGCTGAGGCATAACGAAGAAGTGCGCAGGCGCTGGCAGACCCGTATTCAGTACCTGCTGGTGG
ACGAATATCAAGACACCAACGGCAGTCAGTATGAGCTGGTAAAACTGTTGGTGGGCGAACGTGCCCGGTTTACTGTGGTG
GGGGACGACGACCAGTCAATCTATTCCTGGCGCGGTGCCAAGCCCCAAAACCTGGTGCTCCTTGGCAAGGATTTTCCGAG
CCTTAAGCTTATCAAGCTGGAGCAGAACTACCGCTCCAGTCAGCGCATTTTGCGCGCGGCCAACATCCTTATTGCCAACA
ATCCCCATGTCTACGACAAGTCACTGTTTTCTGAGCTTGCCTACGGCGAGCCGATGCGGGTTATCACGGCGCTGAATGAA
GAGCAGGAAGCTGAGCGGGTGGTGGCTGAAATTATTCGCCATCGCTTCGTAGGGAAAACCAGTTATGGCGACTACGCCAT
TCTCTATCGCGGCAACCATCAATCGCGTCTGATAGAGAGAGCGCTGATGACCAACCGCATTCCCTACAAACTCAGTGGTG
GCACGTCCTTTTTTGCCCGTTCTGAAATCAAGGACATCATGGCCTATCTGCGGCTGGTGGTGAATCCAGATGATGACAAT
GCGTTTTTACGGATAGTCAACCTGCCCAAACGCGGCATCGGCCCGGCAACTCTGGAGCGCTTGGGTAACTTTGCCAACAG
CAAACATATTTCCATGTTTGCGGCTATTTTTGAGCCTGAGCTGGAGCTCCATCTGGGTAACAGTGCCCTGTCGGCGCTGG
AGCAATTTGGCCGCTTTATCGTTGAAACCGGAGACATTGCTGAGCGCGGTGAAGGCGTGGATGCGATCAAGCAGCTCATC
CGCAACATCAATTACGAGGACTTCCTCTACGAGACCAGCCCCAGCGCCAAAGCGGCAGAAATGCGGATGAAGAATATCTC
CGAGCTGTATCGCTGGGTCACTGAGATGCTCAATGGGGACGATCTGGACGAGCCAATGACGCTGCCGGAGGTGGTGGGCC
GTTTGACCCTCAGAGACATGATGGAGCGTAACAGCGAAGACGAAGCCGGCGATCAGGTACAGTTGATGACCCTCCATGCC
TCTAAAGGATTGGAGTTTCCCTACGTGTTTATGATTGGCATGGAAGAGCGTATTCTGCCGCACCAGACCAGCATAGATGA
AGACAACGTGGAAGAGGAACGGCGGCTTGCCTATGTGGGGGTGACCCGTGCGCAGCGGGAGCTTTGGTTTACCCTGTGCC
GTGAGCGGCGTCAGTTCGGCGAAACCATGCGCTGCGAGCCCAGCCGTTTTCTGGACGAGCTACCCCAGGATGACTTGATT
TGGGAGAGTCGCAAGCAGCCCCAAACAGAGCAACAACGGCAGGAAACGGGCAAAGCCAATATCGCCAATATCAGGGCCAT
GTTTAAAAAATAA

Upstream 100 bases:

>100_bases
GACAAAACATTTGGCAAGCGCTGCAGGGTCGCCTGTACGCTTTGGGATCTGCTATGATCGCCAGTCCTCTGTTTGGTTAA
CCCGGTTGAATATCGCCCCC

Downstream 100 bases:

>100_bases
TGACTCAGGATATCAGGCGCAGACGCTGCTCTGCGCCTGATGTAGTGGCTGCAATTGTTGAATGTGATCGATACCTGCCT
GCACCAAACTCTGGCGGGTG

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYQARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEILKREHKAVGLKAGFSLFDDQDTLALLKELTEKELQEDKDLLKLLATSISNWKGALVIPDHARKIAKDER
EQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRHNEEVRRRWQTRIQYLLVDEYQDTNGSQYELVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPSLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVITALNE
EQEAERVVAEIIRHRFVGKTSYGDYAILYRGNHQSRLIERALMTNRIPYKLSGGTSFFARSEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANSKHISMFAAIFEPELELHLGNSALSALEQFGRFIVETGDIAERGEGVDAIKQLI
RNINYEDFLYETSPSAKAAEMRMKNISELYRWVTEMLNGDDLDEPMTLPEVVGRLTLRDMMERNSEDEAGDQVQLMTLHA
SKGLEFPYVFMIGMEERILPHQTSIDEDNVEEERRLAYVGVTRAQRELWFTLCRERRQFGETMRCEPSRFLDELPQDDLI
WESRKQPQTEQQRQETGKANIANIRAMFKK

Sequences:

>Translated_670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYQARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEILKREHKAVGLKAGFSLFDDQDTLALLKELTEKELQEDKDLLKLLATSISNWKGALVIPDHARKIAKDER
EQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRHNEEVRRRWQTRIQYLLVDEYQDTNGSQYELVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPSLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVITALNE
EQEAERVVAEIIRHRFVGKTSYGDYAILYRGNHQSRLIERALMTNRIPYKLSGGTSFFARSEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANSKHISMFAAIFEPELELHLGNSALSALEQFGRFIVETGDIAERGEGVDAIKQLI
RNINYEDFLYETSPSAKAAEMRMKNISELYRWVTEMLNGDDLDEPMTLPEVVGRLTLRDMMERNSEDEAGDQVQLMTLHA
SKGLEFPYVFMIGMEERILPHQTSIDEDNVEEERRLAYVGVTRAQRELWFTLCRERRQFGETMRCEPSRFLDELPQDDLI
WESRKQPQTEQQRQETGKANIANIRAMFKK
>Mature_670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYQARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEILKREHKAVGLKAGFSLFDDQDTLALLKELTEKELQEDKDLLKLLATSISNWKGALVIPDHARKIAKDER
EQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRHNEEVRRRWQTRIQYLLVDEYQDTNGSQYELVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPSLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVITALNE
EQEAERVVAEIIRHRFVGKTSYGDYAILYRGNHQSRLIERALMTNRIPYKLSGGTSFFARSEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANSKHISMFAAIFEPELELHLGNSALSALEQFGRFIVETGDIAERGEGVDAIKQLI
RNINYEDFLYETSPSAKAAEMRMKNISELYRWVTEMLNGDDLDEPMTLPEVVGRLTLRDMMERNSEDEAGDQVQLMTLHA
SKGLEFPYVFMIGMEERILPHQTSIDEDNVEEERRLAYVGVTRAQRELWFTLCRERRQFGETMRCEPSRFLDELPQDDLI
WESRKQPQTEQQRQETGKANIANIRAMFKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=67.9640718562874, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=641, Percent_Identity=39.1575663026521, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1787196, Length=364, Percent_Identity=28.2967032967033, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=711, Percent_Identity=28.1293952180028, Blast_Score=219, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76961; Mature: 76961

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYQARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEILKREHKAVGLKAGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHH
TEKELQEDKDLLKLLATSISNWKGALVIPDHARKIAKDEREQLFALLYQRYAQHMKAYNA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRHNEEVRRRWQTRIQYLLVDEYQDTNGSQYELVKLLVGERARFTVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPSLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCCHHH
SLFSELAYGEPMRVITALNEEQEAERVVAEIIRHRFVGKTSYGDYAILYRGNHQSRLIER
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALMTNRIPYKLSGGTSFFARSEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANSKHISMFAAIFEPELELHLGNSALSALEQFGRFIVETGDIAERGEGVDAIKQLI
HHHHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHEECCCHHHCCCCHHHHHHHH
RNINYEDFLYETSPSAKAAEMRMKNISELYRWVTEMLNGDDLDEPMTLPEVVGRLTLRDM
HHCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEAGDQVQLMTLHASKGLEFPYVFMIGMEERILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHH
VTRAQRELWFTLCRERRQFGETMRCEPSRFLDELPQDDLIWESRKQPQTEQQRQETGKAN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCH
IANIRAMFKK
HHHHHHHHCC
>Mature Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYQARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEILKREHKAVGLKAGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHH
TEKELQEDKDLLKLLATSISNWKGALVIPDHARKIAKDEREQLFALLYQRYAQHMKAYNA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRHNEEVRRRWQTRIQYLLVDEYQDTNGSQYELVKLLVGERARFTVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPSLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCCHHH
SLFSELAYGEPMRVITALNEEQEAERVVAEIIRHRFVGKTSYGDYAILYRGNHQSRLIER
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALMTNRIPYKLSGGTSFFARSEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANSKHISMFAAIFEPELELHLGNSALSALEQFGRFIVETGDIAERGEGVDAIKQLI
HHHHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHEECCCHHHCCCCHHHHHHHH
RNINYEDFLYETSPSAKAAEMRMKNISELYRWVTEMLNGDDLDEPMTLPEVVGRLTLRDM
HHCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEAGDQVQLMTLHASKGLEFPYVFMIGMEERILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHH
VTRAQRELWFTLCRERRQFGETMRCEPSRFLDELPQDDLIWESRKQPQTEQQRQETGKAN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCH
IANIRAMFKK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]