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The map label for this gene is amiB [H]

Identifier: 119776160

GI number: 119776160

Start: 3602939

End: 3604246

Strand: Reverse

Name: amiB [H]

Synonym: Sama_3028

Alternate gene names: 119776160

Gene position: 3604246-3602939 (Counterclockwise)

Preceding gene: 119776161

Following gene: 119776159

Centisome position: 83.7

GC content: 52.98

Gene sequence:

>1308_bases
ATGAATAGAATAATAAGCAGACTTTTTTTCGTTTTAACCCTGCTGTTTTCCGTTGGCGCCTTAGCTGCGAATCAGCTGGA
TGGTGTCAGGATATGGGCCGCACCGGAATCGACCCGGGTAGTGTTTGATCTCACTGCCAAACCCAGTTACACCCATTCTA
CTCTGGTGGGCCCGGACCGACTGGTGTTGGATTTGAGCGATACCCAATCCAAGGTGGCGCTCGGCAAAATCGCCAATAAT
TCCAAGTTGGTGAAGCGGGTTCGCCTGAGCAAACCGCCTGCCAAAGGTATTCTGCGGCTCGTTATCGACCTTAACCGGCC
GGTGAATGCCAACCTGTTTTCGTTACCGCCAACGGCCCCCTATGGCAACCGTCTTGTGGTAGACCTTGAAGAAAAAACAA
GTGAAAGCAAGGCGATAGTAGCACCGCCTTCGTCTTTGCGTGACATTATCGTTGCCATTGATGCAGGACACGGCGGTGAC
GACCCGGGCTCTATCGGCCCCTCAGGTCTGTATGAGAAAAAAATCGCCTTTGAAATTGCCAAACGGGTGCAGGCCAAGAT
AGATGCCACCCCGGGTATGAAAGCGGTATTAACCCGTACCGGTGATTACTACATTCATCCGAACAAGCGCTCGGAGCTGG
CCCGCAAACACCGCGCCGATCTGCTGGTATCCATTCACGCCGATGCGTTCACGTCGCCCAATCCACGCGGTGCGTCAGTG
TGGGTGCTGTCTATGCGCCGAGCCAACAGCGAAATTGGTCGCTGGCTTGAGCAAAAAGAAAAGCACTCAGAGCTTTTGGG
GGGGGCCGGTGAAATTATTCAGAATGCCGATAACGAGCAGTATCTGGCCATGACATTGCTGGATATGTCCATGGACAGAT
CCATGGCTATCAGTCACAGCATTGCGGGCGATGTGCTCAAAGAGCTGGGCAAGGTGACAGACCTGCATAAGAACTTGCCC
GAATCAGCGAGCTTGGCAGCGCTGAAATCCCCAGATATACCCTCTATTCTGGTGGAGACCGGATTTATCTCTAACCCCAA
AGAGGAAAGCCTGCTAAAGAGCAGTTCACATCAGGAACGCCTTGCCAAAGCGATACACACGGGTGTGCTGAGGTATTTTG
AGACCAACCCGCCCAGTGACTCGCTTATCGCCAGCCGCGCCAACAGCCAGCACAAGGTCAGTCGCGGCGAGTCTTTATCT
GTGATTGCAGCCCAATATGGCATTTCTGTCATCAGTCTCAAACAGGCGAATAACCTTAAGTCCGACACCCTGCGTGTGGG
CCAAAAGCTGGTGATCCCCAAAGGATAA

Upstream 100 bases:

>100_bases
CCGATTTACACCTGCATTTGGTATATGCGGGCACTGGACGAGAGCTCAGTATCCAGGCTGGTTCTGCAGCCGGAAGAGCA
GTGATAGAAAATCTCAGCGA

Downstream 100 bases:

>100_bases
ACAAGGAATACCCCATGGCAATTCAGGTATTGCCACCTCAGTTGGCAAACCAGATTGCGGCTGGTGAGGTGGTAGAGCGC
CCAGCCTCGGTGATAAAAGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNRIISRLFFVLTLLFSVGALAANQLDGVRIWAAPESTRVVFDLTAKPSYTHSTLVGPDRLVLDLSDTQSKVALGKIANN
SKLVKRVRLSKPPAKGILRLVIDLNRPVNANLFSLPPTAPYGNRLVVDLEEKTSESKAIVAPPSSLRDIIVAIDAGHGGD
DPGSIGPSGLYEKKIAFEIAKRVQAKIDATPGMKAVLTRTGDYYIHPNKRSELARKHRADLLVSIHADAFTSPNPRGASV
WVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNADNEQYLAMTLLDMSMDRSMAISHSIAGDVLKELGKVTDLHKNLP
ESASLAALKSPDIPSILVETGFISNPKEESLLKSSSHQERLAKAIHTGVLRYFETNPPSDSLIASRANSQHKVSRGESLS
VIAAQYGISVISLKQANNLKSDTLRVGQKLVIPKG

Sequences:

>Translated_435_residues
MNRIISRLFFVLTLLFSVGALAANQLDGVRIWAAPESTRVVFDLTAKPSYTHSTLVGPDRLVLDLSDTQSKVALGKIANN
SKLVKRVRLSKPPAKGILRLVIDLNRPVNANLFSLPPTAPYGNRLVVDLEEKTSESKAIVAPPSSLRDIIVAIDAGHGGD
DPGSIGPSGLYEKKIAFEIAKRVQAKIDATPGMKAVLTRTGDYYIHPNKRSELARKHRADLLVSIHADAFTSPNPRGASV
WVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNADNEQYLAMTLLDMSMDRSMAISHSIAGDVLKELGKVTDLHKNLP
ESASLAALKSPDIPSILVETGFISNPKEESLLKSSSHQERLAKAIHTGVLRYFETNPPSDSLIASRANSQHKVSRGESLS
VIAAQYGISVISLKQANNLKSDTLRVGQKLVIPKG
>Mature_435_residues
MNRIISRLFFVLTLLFSVGALAANQLDGVRIWAAPESTRVVFDLTAKPSYTHSTLVGPDRLVLDLSDTQSKVALGKIANN
SKLVKRVRLSKPPAKGILRLVIDLNRPVNANLFSLPPTAPYGNRLVVDLEEKTSESKAIVAPPSSLRDIIVAIDAGHGGD
DPGSIGPSGLYEKKIAFEIAKRVQAKIDATPGMKAVLTRTGDYYIHPNKRSELARKHRADLLVSIHADAFTSPNPRGASV
WVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNADNEQYLAMTLLDMSMDRSMAISHSIAGDVLKELGKVTDLHKNLP
ESASLAALKSPDIPSILVETGFISNPKEESLLKSSSHQERLAKAIHTGVLRYFETNPPSDSLIASRANSQHKVSRGESLS
VIAAQYGISVISLKQANNLKSDTLRVGQKLVIPKG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=422, Percent_Identity=37.2037914691943, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082163, Length=382, Percent_Identity=35.0785340314136, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=40.4444444444444, Blast_Score=168, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 47227; Mature: 47227

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIISRLFFVLTLLFSVGALAANQLDGVRIWAAPESTRVVFDLTAKPSYTHSTLVGPDR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCEECCCCE
LVLDLSDTQSKVALGKIANNSKLVKRVRLSKPPAKGILRLVIDLNRPVNANLFSLPPTAP
EEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHEEEEECCCCCCCCEEECCCCCC
YGNRLVVDLEEKTSESKAIVAPPSSLRDIIVAIDAGHGGDDPGSIGPSGLYEKKIAFEIA
CCCEEEEEECCCCCCCCEEECCCHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KRVQAKIDATPGMKAVLTRTGDYYIHPNKRSELARKHRADLLVSIHADAFTSPNPRGASV
HHHHHHCCCCCCCCEEEEECCCEEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCEE
WVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNADNEQYLAMTLLDMSMDRSMAISHS
EEEEEHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEHHHHHCCCHHHHHHH
IAGDVLKELGKVTDLHKNLPESASLAALKSPDIPSILVETGFISNPKEESLLKSSSHQER
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCHHHHH
LAKAIHTGVLRYFETNPPSDSLIASRANSQHKVSRGESLSVIAAQYGISVISLKQANNLK
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCCCCEEEEEHHHCCEEEEEECCCCCC
SDTLRVGQKLVIPKG
HHHHHCCCEEECCCC
>Mature Secondary Structure
MNRIISRLFFVLTLLFSVGALAANQLDGVRIWAAPESTRVVFDLTAKPSYTHSTLVGPDR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCEECCCCE
LVLDLSDTQSKVALGKIANNSKLVKRVRLSKPPAKGILRLVIDLNRPVNANLFSLPPTAP
EEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHEEEEECCCCCCCCEEECCCCCC
YGNRLVVDLEEKTSESKAIVAPPSSLRDIIVAIDAGHGGDDPGSIGPSGLYEKKIAFEIA
CCCEEEEEECCCCCCCCEEECCCHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KRVQAKIDATPGMKAVLTRTGDYYIHPNKRSELARKHRADLLVSIHADAFTSPNPRGASV
HHHHHHCCCCCCCCEEEEECCCEEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCEE
WVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNADNEQYLAMTLLDMSMDRSMAISHS
EEEEEHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEHHHHHCCCHHHHHHH
IAGDVLKELGKVTDLHKNLPESASLAALKSPDIPSILVETGFISNPKEESLLKSSSHQER
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCHHHHH
LAKAIHTGVLRYFETNPPSDSLIASRANSQHKVSRGESLSVIAAQYGISVISLKQANNLK
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCCCCEEEEEHHHCCEEEEEECCCCCC
SDTLRVGQKLVIPKG
HHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]