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The map label for this gene is speE [H]

Identifier: 119775798

GI number: 119775798

Start: 3192162

End: 3193877

Strand: Direct

Name: speE [H]

Synonym: Sama_2666

Alternate gene names: 119775798

Gene position: 3192162-3193877 (Clockwise)

Preceding gene: 119775797

Following gene: 119775799

Centisome position: 74.13

GC content: 56.7

Gene sequence:

>1716_bases
ATGGCGCTGGCACAGGACCTGCCTCAGCCGTTGACCCCGGCCAAACACCTCGGCCGCCTGGATGATGCGCTGCTATTGGG
GATCATGGCGCTTTTGGCCGGGTGCGGCCTTATTTACGAGTATCTGCTGAGCCATTATGCCGGGCGGATCCTCGGCGCCA
TGGAAGCCGCCATTTACACCATGATTGGCCTGATGATTGTAGCCATGGGTGCAGGTGCCTTTGCTGCCCGGGCGATTCGC
AATCCCTTTGTCGGTTTTGCCTTGCTGGAACTGACGGTGGCCCTGCTTGGTGCCTCCAGCGTACTGTTTTGCGCCGCCGC
CATCGGTCTCACCCAGAGATTGCCCCAGGTGCTGGCTGACACCTTTGGTTTGCCGCCGGACCTTTTGCCAACCGGCGGAT
TTCTGGGGCAGATACAACAGCTGATGCAGTATCTGCCCTATCTGGTGGGCACCTTACTTGGGCTGTTAATCGGCATGGAG
ATCCCACTTATTGCCAGAGTTCGCCAGGCACTGTCCGATGAGCATCTGCTGCACAATGCCGGCACCATTTACGGTGCCGA
TTATCTGGGTGCAGGGGTTGGGGCCGCTATCTGGGTGGGTCTGATGCTGGCGATGGATATTCAGCTTGCCGCCGCCCTGA
CGGCCAGTGTGAATCTCTTGGCAGGTTTGGCGTTTATTCTGCGTTTCAGAACGCGTCTGACGGGCTTTACCTGGCTGCTG
CTTGGGCATATTCTGGCATCCTTTGCCCTTCTGCTGTTGGCACTTAAAGGCCCGGCACTGGAGCAGGATTTTAATAATCT
GCTCTACAAAGACCGGGTAATTTACTCGCCGGCAACCCGTTTTCAGCAACTGGTATTCACCGAGCGGCTCAGGGGCAAGG
CGCCCCCTGTGTATTCCCTGTATCTGAATGGCCGATTGCAGTTTTCCACTCAGGATGAGGCCATATATCACGGCTTTCTG
GTGTGGCCGGCAATGATGGCAGCAACCAGACACGACAGAGTACTCATTATCGGTGGCGGTGACGGCATGGCCGCCCGGGA
TGTGCTCAGATGGCAACCAGACGAAGTGGTGTTGATGGACCTGGATGAACAGCTGGTTCGCCTGTTTCAGTCACCGGCTG
AGGATATGCCACCGCGCTTGTCCAACGCACTGCTGACGCAGAATGTCGGGGCACTGCAGGACCCGAGACTCACGCTGGTG
TTTGATGATGCCTTTAATGGCGTAGACAGGCTTATCGAGGCGGGCCGCCACTTTGATGTGGTGATTGTTGACTTGCCGGA
CCCAAGTCATCCGGATCTCTCCAAACTCTATTCGGATCTCTTTTACCGCAAACTAAAAGAGCTGCTGAGCCACGATGGCG
CCATTGCGATTCAATCTACCTCCCCCTGGCATGCCACCCGAGCCTTCCTGAGTGTGGGCAACACCCTTGAGTCGGCTGGA
TTCAACATTGCCCGCTACCACCAAAACGTACCCAGCTTCGGTGAGTGGGGCTGGACGCTCGGCACCCTGTCGGGCTCGGC
CAAAGACAAGGTGATGGCGGCAGAGCCCATGCCCCATCCCTGGCTCAGCAAAGAGATGATAAGTGCCTCTTTTGCTTTTC
CACCTGGATTTGAAGAGCGGGCTCTGGCGGTGGACATCAACCGATTCGGCTCACAGGTGATCTACCAATATCATCAGGAA
GCCTGGCAGGAAGAGAGCGGTATCAATTTGTTTTAA

Upstream 100 bases:

>100_bases
CGATGAAATCGAGCGCCAACATAATGTGGGCCTCGCGTCAGTAGAGGTCGCCATCAGTATTGCGATTGCGCTTATTCTCA
CCGGACTGATGGCCTAAGCC

Downstream 100 bases:

>100_bases
CAGGGAAGGCCATCAAAACAGCTCACGTCTTAAAACAGGCTACACACGGGGCGAAAAAACGACCAAGATAACGCTTGACA
TATTATGTCCCGATGTTATC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MALAQDLPQPLTPAKHLGRLDDALLLGIMALLAGCGLIYEYLLSHYAGRILGAMEAAIYTMIGLMIVAMGAGAFAARAIR
NPFVGFALLELTVALLGASSVLFCAAAIGLTQRLPQVLADTFGLPPDLLPTGGFLGQIQQLMQYLPYLVGTLLGLLIGME
IPLIARVRQALSDEHLLHNAGTIYGADYLGAGVGAAIWVGLMLAMDIQLAAALTASVNLLAGLAFILRFRTRLTGFTWLL
LGHILASFALLLLALKGPALEQDFNNLLYKDRVIYSPATRFQQLVFTERLRGKAPPVYSLYLNGRLQFSTQDEAIYHGFL
VWPAMMAATRHDRVLIIGGGDGMAARDVLRWQPDEVVLMDLDEQLVRLFQSPAEDMPPRLSNALLTQNVGALQDPRLTLV
FDDAFNGVDRLIEAGRHFDVVIVDLPDPSHPDLSKLYSDLFYRKLKELLSHDGAIAIQSTSPWHATRAFLSVGNTLESAG
FNIARYHQNVPSFGEWGWTLGTLSGSAKDKVMAAEPMPHPWLSKEMISASFAFPPGFEERALAVDINRFGSQVIYQYHQE
AWQEESGINLF

Sequences:

>Translated_571_residues
MALAQDLPQPLTPAKHLGRLDDALLLGIMALLAGCGLIYEYLLSHYAGRILGAMEAAIYTMIGLMIVAMGAGAFAARAIR
NPFVGFALLELTVALLGASSVLFCAAAIGLTQRLPQVLADTFGLPPDLLPTGGFLGQIQQLMQYLPYLVGTLLGLLIGME
IPLIARVRQALSDEHLLHNAGTIYGADYLGAGVGAAIWVGLMLAMDIQLAAALTASVNLLAGLAFILRFRTRLTGFTWLL
LGHILASFALLLLALKGPALEQDFNNLLYKDRVIYSPATRFQQLVFTERLRGKAPPVYSLYLNGRLQFSTQDEAIYHGFL
VWPAMMAATRHDRVLIIGGGDGMAARDVLRWQPDEVVLMDLDEQLVRLFQSPAEDMPPRLSNALLTQNVGALQDPRLTLV
FDDAFNGVDRLIEAGRHFDVVIVDLPDPSHPDLSKLYSDLFYRKLKELLSHDGAIAIQSTSPWHATRAFLSVGNTLESAG
FNIARYHQNVPSFGEWGWTLGTLSGSAKDKVMAAEPMPHPWLSKEMISASFAFPPGFEERALAVDINRFGSQVIYQYHQE
AWQEESGINLF
>Mature_570_residues
ALAQDLPQPLTPAKHLGRLDDALLLGIMALLAGCGLIYEYLLSHYAGRILGAMEAAIYTMIGLMIVAMGAGAFAARAIRN
PFVGFALLELTVALLGASSVLFCAAAIGLTQRLPQVLADTFGLPPDLLPTGGFLGQIQQLMQYLPYLVGTLLGLLIGMEI
PLIARVRQALSDEHLLHNAGTIYGADYLGAGVGAAIWVGLMLAMDIQLAAALTASVNLLAGLAFILRFRTRLTGFTWLLL
GHILASFALLLLALKGPALEQDFNNLLYKDRVIYSPATRFQQLVFTERLRGKAPPVYSLYLNGRLQFSTQDEAIYHGFLV
WPAMMAATRHDRVLIIGGGDGMAARDVLRWQPDEVVLMDLDEQLVRLFQSPAEDMPPRLSNALLTQNVGALQDPRLTLVF
DDAFNGVDRLIEAGRHFDVVIVDLPDPSHPDLSKLYSDLFYRKLKELLSHDGAIAIQSTSPWHATRAFLSVGNTLESAGF
NIARYHQNVPSFGEWGWTLGTLSGSAKDKVMAAEPMPHPWLSKEMISASFAFPPGFEERALAVDINRFGSQVIYQYHQEA
WQEESGINLF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786312, Length=202, Percent_Identity=31.1881188118812, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI212645616, Length=267, Percent_Identity=29.5880149812734, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323175, Length=276, Percent_Identity=25.7246376811594, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325326, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24645443, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=27.9620853080569, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 62499; Mature: 62367

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAQDLPQPLTPAKHLGRLDDALLLGIMALLAGCGLIYEYLLSHYAGRILGAMEAAIYT
CCCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIGLMIVAMGAGAFAARAIRNPFVGFALLELTVALLGASSVLFCAAAIGLTQRLPQVLAD
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGLPPDLLPTGGFLGQIQQLMQYLPYLVGTLLGLLIGMEIPLIARVRQALSDEHLLHNA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCC
GTIYGADYLGAGVGAAIWVGLMLAMDIQLAAALTASVNLLAGLAFILRFRTRLTGFTWLL
CCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHILASFALLLLALKGPALEQDFNNLLYKDRVIYSPATRFQQLVFTERLRGKAPPVYSL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHCCCCCCEEEE
YLNGRLQFSTQDEAIYHGFLVWPAMMAATRHDRVLIIGGGDGMAARDVLRWQPDEVVLMD
EECCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEC
LDEQLVRLFQSPAEDMPPRLSNALLTQNVGALQDPRLTLVFDDAFNGVDRLIEAGRHFDV
CHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEE
VIVDLPDPSHPDLSKLYSDLFYRKLKELLSHDGAIAIQSTSPWHATRAFLSVGNTLESAG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHC
FNIARYHQNVPSFGEWGWTLGTLSGSAKDKVMAAEPMPHPWLSKEMISASFAFPPGFEER
CHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
ALAVDINRFGSQVIYQYHQEAWQEESGINLF
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALAQDLPQPLTPAKHLGRLDDALLLGIMALLAGCGLIYEYLLSHYAGRILGAMEAAIYT
CCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIGLMIVAMGAGAFAARAIRNPFVGFALLELTVALLGASSVLFCAAAIGLTQRLPQVLAD
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGLPPDLLPTGGFLGQIQQLMQYLPYLVGTLLGLLIGMEIPLIARVRQALSDEHLLHNA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCC
GTIYGADYLGAGVGAAIWVGLMLAMDIQLAAALTASVNLLAGLAFILRFRTRLTGFTWLL
CCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHILASFALLLLALKGPALEQDFNNLLYKDRVIYSPATRFQQLVFTERLRGKAPPVYSL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHCCCCCCEEEE
YLNGRLQFSTQDEAIYHGFLVWPAMMAATRHDRVLIIGGGDGMAARDVLRWQPDEVVLMD
EECCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEC
LDEQLVRLFQSPAEDMPPRLSNALLTQNVGALQDPRLTLVFDDAFNGVDRLIEAGRHFDV
CHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEE
VIVDLPDPSHPDLSKLYSDLFYRKLKELLSHDGAIAIQSTSPWHATRAFLSVGNTLESAG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHC
FNIARYHQNVPSFGEWGWTLGTLSGSAKDKVMAAEPMPHPWLSKEMISASFAFPPGFEER
CHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
ALAVDINRFGSQVIYQYHQEAWQEESGINLF
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]