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The map label for this gene is tesA [H]

Identifier: 119775762

GI number: 119775762

Start: 3149625

End: 3150221

Strand: Reverse

Name: tesA [H]

Synonym: Sama_2630

Alternate gene names: 119775762

Gene position: 3150221-3149625 (Counterclockwise)

Preceding gene: 119775763

Following gene: 119775761

Centisome position: 73.16

GC content: 49.75

Gene sequence:

>597_bases
GTGCGTATTCTGTCAGTGTTTTTTATCCTGCTGTTTTTATCGGCCTGCGGCGGGCCGAGATTGCCTTTTCTGGGTGAAAA
TGCCCGCATTTTGGCCTTCGGTGACAGTTTAACTTTTGGTATTGGTGCCAGTGACGGCATGGACTATCCAGCCCACCTTT
CCCGTCTGTGCGACTGTGACGTGATTAACGCCGGGGTATCGGGGGAAACAACCACCGAAGGTCTGGCTCGACTGGCTGAA
TTGCTTGATGAAACCCAACCGGATCTGCTTATCTTGCTGGAAGGTGGCAATGATATCCTCAGAAATCAGGATAAAGCACA
GCTTAAGCGCAATTTGGAAGCTATGATTAGGGAAGCTCAGGGAAGGCAGGTGCCTGTGCTTTTGATTGCTGTACCAGACC
GAAAGGTGTTTTTATCCCCTTTACCCTTGTATCAGGAACTGGCTGAGCAATTTTCCCTGCCACTGCTTGAGGATACGCTA
CCGCAGCTCCTGTCACAAAGCGCCATGAAATCGGACACAGTTCATTTAAATGACGCCGGATATCAGCTTTTGGCGGAAGA
AATTTACCAGCTTGCGAGCGAGAGTGGAGCCTTTTAA

Upstream 100 bases:

>100_bases
CTGGGACTGAAATACTGATAAGCCACTGAAATTGAAAAGCCGGACTTGTGTCCGGCTTTTTTTTGGGCGATAACAGACGC
ATTAGTCCGGGGAGTAAAAA

Downstream 100 bases:

>100_bases
GGCCGCTTTCTGAAAATTGCTGAAACGGACGGAAATCAGCTTGACCATGGGCAGGATTTTATGGGTAAATTCCCCGGTAA
TAGCAGCCAGGGCGGGTTTA

Product: lipolytic enzyme, G-D-S-L

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MRILSVFFILLFLSACGGPRLPFLGENARILAFGDSLTFGIGASDGMDYPAHLSRLCDCDVINAGVSGETTTEGLARLAE
LLDETQPDLLILLEGGNDILRNQDKAQLKRNLEAMIREAQGRQVPVLLIAVPDRKVFLSPLPLYQELAEQFSLPLLEDTL
PQLLSQSAMKSDTVHLNDAGYQLLAEEIYQLASESGAF

Sequences:

>Translated_198_residues
MRILSVFFILLFLSACGGPRLPFLGENARILAFGDSLTFGIGASDGMDYPAHLSRLCDCDVINAGVSGETTTEGLARLAE
LLDETQPDLLILLEGGNDILRNQDKAQLKRNLEAMIREAQGRQVPVLLIAVPDRKVFLSPLPLYQELAEQFSLPLLEDTL
PQLLSQSAMKSDTVHLNDAGYQLLAEEIYQLASESGAF
>Mature_198_residues
MRILSVFFILLFLSACGGPRLPFLGENARILAFGDSLTFGIGASDGMDYPAHLSRLCDCDVINAGVSGETTTEGLARLAE
LLDETQPDLLILLEGGNDILRNQDKAQLKRNLEAMIREAQGRQVPVLLIAVPDRKVFLSPLPLYQELAEQFSLPLLEDTL
PQLLSQSAMKSDTVHLNDAGYQLLAEEIYQLASESGAF

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=167, Percent_Identity=29.3413173652695, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21634; Mature: 21634

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILSVFFILLFLSACGGPRLPFLGENARILAFGDSLTFGIGASDGMDYPAHLSRLCDCD
CHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHCCCH
VINAGVSGETTTEGLARLAELLDETQPDLLILLEGGNDILRNQDKAQLKRNLEAMIREAQ
HCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHC
GRQVPVLLIAVPDRKVFLSPLPLYQELAEQFSLPLLEDTLPQLLSQSAMKSDTVHLNDAG
CCCCCEEEEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCHH
YQLLAEEIYQLASESGAF
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRILSVFFILLFLSACGGPRLPFLGENARILAFGDSLTFGIGASDGMDYPAHLSRLCDCD
CHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHCCCH
VINAGVSGETTTEGLARLAELLDETQPDLLILLEGGNDILRNQDKAQLKRNLEAMIREAQ
HCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHC
GRQVPVLLIAVPDRKVFLSPLPLYQELAEQFSLPLLEDTLPQLLSQSAMKSDTVHLNDAG
CCCCCEEEEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCHH
YQLLAEEIYQLASESGAF
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]