| Definition | Shewanella amazonensis SB2B chromosome, complete genome. |
|---|---|
| Accession | NC_008700 |
| Length | 4,306,142 |
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The map label for this gene is metE [H]
Identifier: 119775759
GI number: 119775759
Start: 3145316
End: 3147586
Strand: Reverse
Name: metE [H]
Synonym: Sama_2627
Alternate gene names: 119775759
Gene position: 3147586-3145316 (Counterclockwise)
Preceding gene: 119775761
Following gene: 119775758
Centisome position: 73.1
GC content: 57.38
Gene sequence:
>2271_bases ATGCAATTAAATAGTCTTGGCTTTCCACGGATAGGACGTCGCCGTGAGTTGAAGTTCGCCCTCGAGCAATACTGGCGTGG AGAGTTGTCCCGGAATGAGCTGGTGGCGCAGGCCAAGGCACTGCGGCAGACTCACTGGCAGTGGCAGGCTGATGCCGGCG TGGAACGTTTGCCGGTGGGGGACTTTGCATACTACGATCAGGTGCTGACTCTGGCGGCCACCCTGAATGTCATTCCCGAG CGCCACCGCGATGGCGACAAGGTCGACCTGGATACCCTGTTCCGGGTTGCCCGAGGCCGTGCGCCAAGCGGCAAAGCGGC CGCGGCGGCAGAAATGACCAAGTATTTCAACACCAACTATCACTACATAGTGCCCGAACTTCACCCCAACCAAACCTTCT CCATCGCCTGGACACAGCTGTTTGATGAGGTCGAAGAAGTGAAAGCGCTGGGCTATGACCCCAAACCCTGCATTCTGGGC CCCGTGTCTTTCCTGTATTTGTCGAAGGCCTACGGCAGTGAGTTTGACAAATTAAGCTTGCTGCCCCAGCTGCTGGTTAC CTATGCTGAGCTGCTCGCCCGCTTTGCCGCCCAGGGCGTGAGCTGGGTACAAATCGAAGAGCCTGTTCTTGCGCTGGATC TGGATGAAAGCTGGCAGGCAGCCTTTGCCACCGCATACCAGGCTTTTACCTGTGTGTCGGTTAAACTGCTGCTGACCACT TACTACGGCTCTGTGGCACACCACGCCGACATTATTCAGACCCTGCCGGTGGCGGGTTTGCATCTGGATCTGGTTTCTGC CCCGGAGCAGCTCGAGGTTTTCCTTCCCAGGCTTGGGCAGCAGCAGGTACTGAGTCTTGGCCTTATCAATGGCCGCAATG TCTGGGCTGAAGATCTGGATTTGATTGCCGAGCGCATTGCACCTGTTGCCAGAGAACTGGGCGATCGCCTGTGGCTTGCC ACCTCCTGCTCGCTGCTGCACACACCGGTGGACCTCGATGTGGAAACCGCACTTAAGCCTGAGCTCAAGCGCCAGCTCGC TTTTGCCAGGCAAAAGCTGCTGGAGCTGAGAAGCCTCAATACCCTGCTAACCGTGCCCAATGGCGCAGACGCCGCTGCCA TTGCCGCCGAGTGCGTGGCCCGCCGCACTGCGCGTGCTCAGGCCGCCGATGCTGCAGTTGCCGCCAGACTGGATGCACTG ACCAAAGCAGATTTTGAACGTCAAAGCGCGTTTCCAGAGCGTCAGCAACAGCAGCAACAGCGACTTAAGTTGCCTCTGCT GCCAACCACTACCATAGGCTCCTTCCCGCAGACGCCGGCAATTCGTGGCCTGCGTAACCGCTTTCGAAAAGGTGAAATCA GCGCCCTTGAGTACGACAACCAGCTCAGACAGGTGTGCCGCAATACCATTGACCGCCAACTGAAACTCGGTATCGATGTG CTGGTACACGGTGAGGCCGAGCGTAACGATATGGTGGAATACTTTGGCGAGCAGCTCGAGGGTGTCGGCTTTACCAAACA TGGCTGGGTGCAGAGTTATGGCAGTCGCTGTGTGAAACCGCCTTTGATTTACGGTGATGTGTCGCGTCCCAAACCTATGA CACTGGACTGGGCTGAATATGCCCAGAGCCTCACCGATAAGCCGGTGAAAGGCATGTTGACGGGGCCTGTGACCATTCTG CACTGGTCTTTTGCCAGGGAAGATATCAGCCGCGACACGCTTTGTAAGCAGATTGCGCTGGCCATACGGGATGAAGTCGC TGATCTTGAAAGGGTAGGTATCGCCATTATCCAGATTGACGAGCCGGCCTTCCGTGAAGGTTTGCCGCTGCGCCGTTGTG ACTGGAAGGCCTATCTCGACTGGGCGGTGGACTCCTTTAAGCTGAGCGCTGCCTGCGTGCGGGATAACACCCAAATTCAC ACCCACATGTGCTATAGCGAATTTAACGACACCATAGCTGCTATCGCCGCCATGGATGCCGATGTTATCACCATCGAGAC CAGTCGCTCGGCCATGGAGCTGCTGCGAGCCTTTGAAGACTTTGAGTATCCTGCTGAAATTGGCCCGGGTGTGTACGATA TCCACTCGCCCAACACGCCAGATGTTGGGGCCATGGTGACGCTGATGGAAAGGGCAGCCAGGCGTATTCCGCTCAGGCAG CTGTGGGTCAATCCGGACTGCGGCCTGAAGACCCGTACCTGGGATGAGGTAGAACCAGCCCTTCGTAACATGGTTGACGC CACCCGTGAGCTGCGGCGTCGTCTGGGCTAA
Upstream 100 bases:
>100_bases TACCACAATAAACTTCTAGACGTCCAGACGCCTATTAAAAATTGTTTAACAGGTGTTCCTCTTTGAAATGTGCTCATATT AAACCAATAAGGTATACAAT
Downstream 100 bases:
>100_bases GTCTTACGGCTGAAATCAGAGACTATACTGAGGTAACAGTATCGGGGTGGCTGTCTAAGGCTGCATCCCGTCTTAAGGAG GCTTGCCATGAATCATGTCT
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 756; Mature: 756
Protein sequence:
>756_residues MQLNSLGFPRIGRRRELKFALEQYWRGELSRNELVAQAKALRQTHWQWQADAGVERLPVGDFAYYDQVLTLAATLNVIPE RHRDGDKVDLDTLFRVARGRAPSGKAAAAAEMTKYFNTNYHYIVPELHPNQTFSIAWTQLFDEVEEVKALGYDPKPCILG PVSFLYLSKAYGSEFDKLSLLPQLLVTYAELLARFAAQGVSWVQIEEPVLALDLDESWQAAFATAYQAFTCVSVKLLLTT YYGSVAHHADIIQTLPVAGLHLDLVSAPEQLEVFLPRLGQQQVLSLGLINGRNVWAEDLDLIAERIAPVARELGDRLWLA TSCSLLHTPVDLDVETALKPELKRQLAFARQKLLELRSLNTLLTVPNGADAAAIAAECVARRTARAQAADAAVAARLDAL TKADFERQSAFPERQQQQQQRLKLPLLPTTTIGSFPQTPAIRGLRNRFRKGEISALEYDNQLRQVCRNTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLEGVGFTKHGWVQSYGSRCVKPPLIYGDVSRPKPMTLDWAEYAQSLTDKPVKGMLTGPVTIL HWSFAREDISRDTLCKQIALAIRDEVADLERVGIAIIQIDEPAFREGLPLRRCDWKAYLDWAVDSFKLSAACVRDNTQIH THMCYSEFNDTIAAIAAMDADVITIETSRSAMELLRAFEDFEYPAEIGPGVYDIHSPNTPDVGAMVTLMERAARRIPLRQ LWVNPDCGLKTRTWDEVEPALRNMVDATRELRRRLG
Sequences:
>Translated_756_residues MQLNSLGFPRIGRRRELKFALEQYWRGELSRNELVAQAKALRQTHWQWQADAGVERLPVGDFAYYDQVLTLAATLNVIPE RHRDGDKVDLDTLFRVARGRAPSGKAAAAAEMTKYFNTNYHYIVPELHPNQTFSIAWTQLFDEVEEVKALGYDPKPCILG PVSFLYLSKAYGSEFDKLSLLPQLLVTYAELLARFAAQGVSWVQIEEPVLALDLDESWQAAFATAYQAFTCVSVKLLLTT YYGSVAHHADIIQTLPVAGLHLDLVSAPEQLEVFLPRLGQQQVLSLGLINGRNVWAEDLDLIAERIAPVARELGDRLWLA TSCSLLHTPVDLDVETALKPELKRQLAFARQKLLELRSLNTLLTVPNGADAAAIAAECVARRTARAQAADAAVAARLDAL TKADFERQSAFPERQQQQQQRLKLPLLPTTTIGSFPQTPAIRGLRNRFRKGEISALEYDNQLRQVCRNTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLEGVGFTKHGWVQSYGSRCVKPPLIYGDVSRPKPMTLDWAEYAQSLTDKPVKGMLTGPVTIL HWSFAREDISRDTLCKQIALAIRDEVADLERVGIAIIQIDEPAFREGLPLRRCDWKAYLDWAVDSFKLSAACVRDNTQIH THMCYSEFNDTIAAIAAMDADVITIETSRSAMELLRAFEDFEYPAEIGPGVYDIHSPNTPDVGAMVTLMERAARRIPLRQ LWVNPDCGLKTRTWDEVEPALRNMVDATRELRRRLG >Mature_756_residues MQLNSLGFPRIGRRRELKFALEQYWRGELSRNELVAQAKALRQTHWQWQADAGVERLPVGDFAYYDQVLTLAATLNVIPE RHRDGDKVDLDTLFRVARGRAPSGKAAAAAEMTKYFNTNYHYIVPELHPNQTFSIAWTQLFDEVEEVKALGYDPKPCILG PVSFLYLSKAYGSEFDKLSLLPQLLVTYAELLARFAAQGVSWVQIEEPVLALDLDESWQAAFATAYQAFTCVSVKLLLTT YYGSVAHHADIIQTLPVAGLHLDLVSAPEQLEVFLPRLGQQQVLSLGLINGRNVWAEDLDLIAERIAPVARELGDRLWLA TSCSLLHTPVDLDVETALKPELKRQLAFARQKLLELRSLNTLLTVPNGADAAAIAAECVARRTARAQAADAAVAARLDAL TKADFERQSAFPERQQQQQQRLKLPLLPTTTIGSFPQTPAIRGLRNRFRKGEISALEYDNQLRQVCRNTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLEGVGFTKHGWVQSYGSRCVKPPLIYGDVSRPKPMTLDWAEYAQSLTDKPVKGMLTGPVTIL HWSFAREDISRDTLCKQIALAIRDEVADLERVGIAIIQIDEPAFREGLPLRRCDWKAYLDWAVDSFKLSAACVRDNTQIH THMCYSEFNDTIAAIAAMDADVITIETSRSAMELLRAFEDFEYPAEIGPGVYDIHSPNTPDVGAMVTLMERAARRIPLRQ LWVNPDCGLKTRTWDEVEPALRNMVDATRELRRRLG
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=57.7127659574468, Blast_Score=867, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=767, Percent_Identity=45.2411994784876, Blast_Score=656, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 85007; Mature: 85007
Theoretical pI: Translated: 5.83; Mature: 5.83
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLNSLGFPRIGRRRELKFALEQYWRGELSRNELVAQAKALRQTHWQWQADAGVERLPVG CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCC DFAYYDQVLTLAATLNVIPERHRDGDKVDLDTLFRVARGRAPSGKAAAAAEMTKYFNTNY HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC HYIVPELHPNQTFSIAWTQLFDEVEEVKALGYDPKPCILGPVSFLYLSKAYGSEFDKLSL EEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHCCCHHHHHH LPQLLVTYAELLARFAAQGVSWVQIEEPVLALDLDESWQAAFATAYQAFTCVSVKLLLTT HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH YYGSVAHHADIIQTLPVAGLHLDLVSAPEQLEVFLPRLGQQQVLSLGLINGRNVWAEDLD HHHHHHHHHHHHHHCCCCCEEEEHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHH LIAERIAPVARELGDRLWLATSCSLLHTPVDLDVETALKPELKRQLAFARQKLLELRSLN HHHHHHHHHHHHHHCCEEHEECHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCC TLLTVPNGADAAAIAAECVARRTARAQAADAAVAARLDALTKADFERQSAFPERQQQQQQ CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH RLKLPLLPTTTIGSFPQTPAIRGLRNRFRKGEISALEYDNQLRQVCRNTIDRQLKLGIDV HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE LVHGEAERNDMVEYFGEQLEGVGFTKHGWVQSYGSRCVKPPLIYGDVSRPKPMTLDWAEY EEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCEECHHHH AQSLTDKPVKGMLTGPVTILHWSFAREDISRDTLCKQIALAIRDEVADLERVGIAIIQID HHHHCCCCCCCEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC EPAFREGLPLRRCDWKAYLDWAVDSFKLSAACVRDNTQIHTHMCYSEFNDTIAAIAAMDA CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC DVITIETSRSAMELLRAFEDFEYPAEIGPGVYDIHSPNTPDVGAMVTLMERAARRIPLRQ CEEEEECCHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHH LWVNPDCGLKTRTWDEVEPALRNMVDATRELRRRLG EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MQLNSLGFPRIGRRRELKFALEQYWRGELSRNELVAQAKALRQTHWQWQADAGVERLPVG CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCC DFAYYDQVLTLAATLNVIPERHRDGDKVDLDTLFRVARGRAPSGKAAAAAEMTKYFNTNY HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC HYIVPELHPNQTFSIAWTQLFDEVEEVKALGYDPKPCILGPVSFLYLSKAYGSEFDKLSL EEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHCCCHHHHHH LPQLLVTYAELLARFAAQGVSWVQIEEPVLALDLDESWQAAFATAYQAFTCVSVKLLLTT HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH YYGSVAHHADIIQTLPVAGLHLDLVSAPEQLEVFLPRLGQQQVLSLGLINGRNVWAEDLD HHHHHHHHHHHHHHCCCCCEEEEHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHH LIAERIAPVARELGDRLWLATSCSLLHTPVDLDVETALKPELKRQLAFARQKLLELRSLN HHHHHHHHHHHHHHCCEEHEECHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCC TLLTVPNGADAAAIAAECVARRTARAQAADAAVAARLDALTKADFERQSAFPERQQQQQQ CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH RLKLPLLPTTTIGSFPQTPAIRGLRNRFRKGEISALEYDNQLRQVCRNTIDRQLKLGIDV HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE LVHGEAERNDMVEYFGEQLEGVGFTKHGWVQSYGSRCVKPPLIYGDVSRPKPMTLDWAEY EEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCEECHHHH AQSLTDKPVKGMLTGPVTILHWSFAREDISRDTLCKQIALAIRDEVADLERVGIAIIQID HHHHCCCCCCCEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC EPAFREGLPLRRCDWKAYLDWAVDSFKLSAACVRDNTQIHTHMCYSEFNDTIAAIAAMDA CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC DVITIETSRSAMELLRAFEDFEYPAEIGPGVYDIHSPNTPDVGAMVTLMERAARRIPLRQ CEEEEECCHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHH LWVNPDCGLKTRTWDEVEPALRNMVDATRELRRRLG EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12368813 [H]