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The map label for this gene is dacA [H]

Identifier: 119775724

GI number: 119775724

Start: 3100253

End: 3101431

Strand: Direct

Name: dacA [H]

Synonym: Sama_2592

Alternate gene names: 119775724

Gene position: 3100253-3101431 (Clockwise)

Preceding gene: 119775723

Following gene: 119775725

Centisome position: 72.0

GC content: 52.08

Gene sequence:

>1179_bases
ATGATGAAAGTAATCAAACCTAGCGCCCCAAAATCGATGTTGCTCCTGGCCATTATGTCTGCCAGCGCCTTTGCAGCACC
ACCGACCGTCGTTCCAGATGCCCCTTCCGTGGCCGCCAAAGCTTATGTGTTGATGGATTATCACACCGGCCAGATCATTG
CTGAAGAACATGCTTATGAGCAGCTGAATCCGGCCAGCCTTACCAAGATGATGACCAGCTATGTGATTGGTCATGAAATG
AAAGTGGGCAACATCTCACCGGAAGATGACGTCACCATCACCAAAAACGCCTGGTCAAAGAACTTTCCCGATTCCTCAAA
GATGTTCATCGAGGTAGGCAAAACAGTAAAAGCCGCCGATTTGAACCGTGGCATCATCATCCAGTCCGGTAACGACGCCT
GTGTGGCCATGGCTGAGCACATTGCCGGTACCGAAGATGCTTTCGTTGATATGATGAACTCCTGGGCGAAGCAACTCGGT
ATGACCGACAGCTACTTTGAAAACAGCCACGGTCTGGACTCTGACAACCACAAAACCACCGCCTATGACATGGCCATTCT
GGGTGCCGCCCTTATCCGTGACGTGCCGGAAGAGTACCGCATCTATTCCGAAAAAGAGTTCACCTTTAACGGTATCAAGC
AATACAACCGTAACGGCCTCTTATGGGACAAGAGCATGAACGTGGACGGTATCAAGACTGGCCACACCTCTGGCGCGGGT
TATAACCTGGTGGCCTCTGCCACACAGGACGACATGCGTTTGATTTCTGTGGTCATGGGCGCCAGCAGCGAGGCTTCCCG
TAAGGCCGAAAGCAAAAAGCTACTGTCCTATGGTTTCCGCTTCTTCGAAACCGTCACCCCCTACAAGGCCGGTGACAGCT
TCGTAAATCAACAAATTTGGTATGGCGATCGTGAAAGTGTCGACCTGGGCGTCGCTACCGACACTCCTCTGACCATTCGT
CGCGGCAAAGGCAAAGAACTTCAGGCCAACTTCGAACTGACCAAGCCATTGGAAGCTCCACTGGCCAAAGGCGAAGTAGT
GGGCCGCGTGTATTTCCAGTTGGATGGCAAAGACATAGCACAATTCCCGCTGGTAACACTGCAGGAAGTGAATGAAGGCA
GCTGGTTCTCCAAGCTGATGGATTACTTCAAGCAGCTCGTCGCCAGCTGGCTGAGCTAA

Upstream 100 bases:

>100_bases
TGAACATGAAGGAACCTTCACTGCAATCCCTTGTCAATTAATGTTAGACTCGACGGGTTTCATACCGTTTACTAAAACCT
CCTGAGAAAACGTGTAGTTA

Downstream 100 bases:

>100_bases
GCCGATTCCCATGAAAATGCCACCTTCGGGTGGCATTTTTTTCGCCCCCAGACCTTGATTGGGCTATAATCGCCGCCATA
TTTGAGGCCCAAGCCCACGA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MMKVIKPSAPKSMLLLAIMSASAFAAPPTVVPDAPSVAAKAYVLMDYHTGQIIAEEHAYEQLNPASLTKMMTSYVIGHEM
KVGNISPEDDVTITKNAWSKNFPDSSKMFIEVGKTVKAADLNRGIIIQSGNDACVAMAEHIAGTEDAFVDMMNSWAKQLG
MTDSYFENSHGLDSDNHKTTAYDMAILGAALIRDVPEEYRIYSEKEFTFNGIKQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVASATQDDMRLISVVMGASSEASRKAESKKLLSYGFRFFETVTPYKAGDSFVNQQIWYGDRESVDLGVATDTPLTIR
RGKGKELQANFELTKPLEAPLAKGEVVGRVYFQLDGKDIAQFPLVTLQEVNEGSWFSKLMDYFKQLVASWLS

Sequences:

>Translated_392_residues
MMKVIKPSAPKSMLLLAIMSASAFAAPPTVVPDAPSVAAKAYVLMDYHTGQIIAEEHAYEQLNPASLTKMMTSYVIGHEM
KVGNISPEDDVTITKNAWSKNFPDSSKMFIEVGKTVKAADLNRGIIIQSGNDACVAMAEHIAGTEDAFVDMMNSWAKQLG
MTDSYFENSHGLDSDNHKTTAYDMAILGAALIRDVPEEYRIYSEKEFTFNGIKQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVASATQDDMRLISVVMGASSEASRKAESKKLLSYGFRFFETVTPYKAGDSFVNQQIWYGDRESVDLGVATDTPLTIR
RGKGKELQANFELTKPLEAPLAKGEVVGRVYFQLDGKDIAQFPLVTLQEVNEGSWFSKLMDYFKQLVASWLS
>Mature_392_residues
MMKVIKPSAPKSMLLLAIMSASAFAAPPTVVPDAPSVAAKAYVLMDYHTGQIIAEEHAYEQLNPASLTKMMTSYVIGHEM
KVGNISPEDDVTITKNAWSKNFPDSSKMFIEVGKTVKAADLNRGIIIQSGNDACVAMAEHIAGTEDAFVDMMNSWAKQLG
MTDSYFENSHGLDSDNHKTTAYDMAILGAALIRDVPEEYRIYSEKEFTFNGIKQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVASATQDDMRLISVVMGASSEASRKAESKKLLSYGFRFFETVTPYKAGDSFVNQQIWYGDRESVDLGVATDTPLTIR
RGKGKELQANFELTKPLEAPLAKGEVVGRVYFQLDGKDIAQFPLVTLQEVNEGSWFSKLMDYFKQLVASWLS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=395, Percent_Identity=52.9113924050633, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787062, Length=386, Percent_Identity=53.6269430051813, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI87082022, Length=376, Percent_Identity=46.5425531914894, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI87082054, Length=259, Percent_Identity=25.0965250965251, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43237; Mature: 43237

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVIKPSAPKSMLLLAIMSASAFAAPPTVVPDAPSVAAKAYVLMDYHTGQIIAEEHAYE
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCEEEHHHHHH
QLNPASLTKMMTSYVIGHEMKVGNISPEDDVTITKNAWSKNFPDSSKMFIEVGKTVKAAD
HCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEECCEEEECC
LNRGIIIQSGNDACVAMAEHIAGTEDAFVDMMNSWAKQLGMTDSYFENSHGLDSDNHKTT
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCH
AYDMAILGAALIRDVPEEYRIYSEKEFTFNGIKQYNRNGLLWDKSMNVDGIKTGHTSGAG
HHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC
YNLVASATQDDMRLISVVMGASSEASRKAESKKLLSYGFRFFETVTPYKAGDSFVNQQIW
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHC
YGDRESVDLGVATDTPLTIRRGKGKELQANFELTKPLEAPLAKGEVVGRVYFQLDGKDIA
CCCCCCEEEEEECCCCEEEECCCCCEEEECCEECCCCCCCCCCCCEEEEEEEEECCCCHH
QFPLVTLQEVNEGSWFSKLMDYFKQLVASWLS
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKVIKPSAPKSMLLLAIMSASAFAAPPTVVPDAPSVAAKAYVLMDYHTGQIIAEEHAYE
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCEEEHHHHHH
QLNPASLTKMMTSYVIGHEMKVGNISPEDDVTITKNAWSKNFPDSSKMFIEVGKTVKAAD
HCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEECCEEEECC
LNRGIIIQSGNDACVAMAEHIAGTEDAFVDMMNSWAKQLGMTDSYFENSHGLDSDNHKTT
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCH
AYDMAILGAALIRDVPEEYRIYSEKEFTFNGIKQYNRNGLLWDKSMNVDGIKTGHTSGAG
HHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC
YNLVASATQDDMRLISVVMGASSEASRKAESKKLLSYGFRFFETVTPYKAGDSFVNQQIW
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHC
YGDRESVDLGVATDTPLTIRRGKGKELQANFELTKPLEAPLAKGEVVGRVYFQLDGKDIA
CCCCCCEEEEEECCCCEEEECCCCCEEEECCEECCCCCCCCCCCCEEEEEEEEECCCCHH
QFPLVTLQEVNEGSWFSKLMDYFKQLVASWLS
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]