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The map label for this gene is holA [H]

Identifier: 119775716

GI number: 119775716

Start: 3092790

End: 3093818

Strand: Direct

Name: holA [H]

Synonym: Sama_2584

Alternate gene names: 119775716

Gene position: 3092790-3093818 (Clockwise)

Preceding gene: 119775715

Following gene: 119775717

Centisome position: 71.82

GC content: 57.73

Gene sequence:

>1029_bases
ATGCGGGTCTATCCTGAGCAGCTGGGCCGCCACCTGACACCGTTAGCCCAATGTTACTGCTTGTTCGGTGACGATGCCTG
GTTGATAGAAAACGCCCGCACTCAGTTATTGATGGCCGCAAGGCAGCAAGGGTTTGAAGAGCGCATCAGCCTCGAGCAGG
ATAACGGCTTCAACTGGAACGACTTGCAAAACGAGTGGCAAGCGTTGAGCCTCTTTGCCAGCCGCCGCATTATCGAACTC
ACCCTGCCCCAGGCCAAACCCGGCGCCGACGGCAGCGCCATGCTGACCAGCCTGATGGCGCAGCCTAATCCGGATCTGCT
GCTTATTCTGCGTGGTCCCAAACTCGCCGCCGAACAAACCAACAGCAAATGGTTTAAAGCCCTCGACAATGTCGGTATTT
ATCTTCCCTGCAACACTCCCGAAGGGCAGCAATTTTTCCGCTGGCTGGATGGCCGCATTGCCGCCAACCGATTGATGCTG
GACGGAGATGCCCGCCAATTACTGGCAACCCTGTACGAAGGGAACCTGCTGGCCGCAGAGCAGGCGGTACAATTGCTGGC
GCTGCTGGCGGCCAATCGACGTATCAGCGCCGAAGAGCTGAGCCAGTATTTCGACGACCAGTCCCGCTTTAACGTGTTTC
AGCTCAGTGATGCCCTGCTTGGAAACCAGCAGCAGCGCGTGGCCCATATACTGGCCCAGCTCAAGGCTGAAGGCACGGCC
TTGCCCATTGTGCTCTGGGCCGTGTTTCGCGAGCTTGCGAGTCTGCTGCAACTGAAAACCGCCATGGTGCAGAGAGAATC
CCTGGCGCCGATGTGGTCAAAACTGCGGATTTGGGATAAGCGCAAGCCACTGTATGAGGCCGCGCTGTCGCGACTGTCAC
TGACACAGATTGAAGCCATGCTGGCCCTCTCATCCACACTGGAGCTCAAACTTAAACAGGGCGGAGAAGAGGACTGGACA
CTGCTGACTCACCTTTGCCTGCTCTTTGATGCCAGTGCCCACAGCGCTCTGGTGGATACCCGGGGTTGA

Upstream 100 bases:

>100_bases
CACTGGCCAAAAGCCGTGAAATGGAGCTGCTGCTCAAAGAAATGCGCCAGCAAGCCGCCGACAACCTTATCCAAACCCTG
TCGTCCCTCGGGGAGCGCTG

Downstream 100 bases:

>100_bases
TATGACTAAGCAGCCTAATGCAAAGCGGCATACAGCTCTGCTCGGCGGCACCTTTGACCCGCCTCACTTTGGCCATATCA
GGCCACTGCTGGATGTGCTT

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRVYPEQLGRHLTPLAQCYCLFGDDAWLIENARTQLLMAARQQGFEERISLEQDNGFNWNDLQNEWQALSLFASRRIIEL
TLPQAKPGADGSAMLTSLMAQPNPDLLLILRGPKLAAEQTNSKWFKALDNVGIYLPCNTPEGQQFFRWLDGRIAANRLML
DGDARQLLATLYEGNLLAAEQAVQLLALLAANRRISAEELSQYFDDQSRFNVFQLSDALLGNQQQRVAHILAQLKAEGTA
LPIVLWAVFRELASLLQLKTAMVQRESLAPMWSKLRIWDKRKPLYEAALSRLSLTQIEAMLALSSTLELKLKQGGEEDWT
LLTHLCLLFDASAHSALVDTRG

Sequences:

>Translated_342_residues
MRVYPEQLGRHLTPLAQCYCLFGDDAWLIENARTQLLMAARQQGFEERISLEQDNGFNWNDLQNEWQALSLFASRRIIEL
TLPQAKPGADGSAMLTSLMAQPNPDLLLILRGPKLAAEQTNSKWFKALDNVGIYLPCNTPEGQQFFRWLDGRIAANRLML
DGDARQLLATLYEGNLLAAEQAVQLLALLAANRRISAEELSQYFDDQSRFNVFQLSDALLGNQQQRVAHILAQLKAEGTA
LPIVLWAVFRELASLLQLKTAMVQRESLAPMWSKLRIWDKRKPLYEAALSRLSLTQIEAMLALSSTLELKLKQGGEEDWT
LLTHLCLLFDASAHSALVDTRG
>Mature_342_residues
MRVYPEQLGRHLTPLAQCYCLFGDDAWLIENARTQLLMAARQQGFEERISLEQDNGFNWNDLQNEWQALSLFASRRIIEL
TLPQAKPGADGSAMLTSLMAQPNPDLLLILRGPKLAAEQTNSKWFKALDNVGIYLPCNTPEGQQFFRWLDGRIAANRLML
DGDARQLLATLYEGNLLAAEQAVQLLALLAANRRISAEELSQYFDDQSRFNVFQLSDALLGNQQQRVAHILAQLKAEGTA
LPIVLWAVFRELASLLQLKTAMVQRESLAPMWSKLRIWDKRKPLYEAALSRLSLTQIEAMLALSSTLELKLKQGGEEDWT
LLTHLCLLFDASAHSALVDTRG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=342, Percent_Identity=38.0116959064327, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38520; Mature: 38520

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYPEQLGRHLTPLAQCYCLFGDDAWLIENARTQLLMAARQQGFEERISLEQDNGFNWN
CCCCHHHHCCCCCHHHHEEEEECCCEEEHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHH
DLQNEWQALSLFASRRIIELTLPQAKPGADGSAMLTSLMAQPNPDLLLILRGPKLAAEQT
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHC
NSKWFKALDNVGIYLPCNTPEGQQFFRWLDGRIAANRLMLDGDARQLLATLYEGNLLAAE
CHHHHHHHHCCCEEEECCCCCHHHHHHHHHCHHHHCEEEECCCHHHHHHHHHCCCCHHHH
QAVQLLALLAANRRISAEELSQYFDDQSRFNVFQLSDALLGNQQQRVAHILAQLKAEGTA
HHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
LPIVLWAVFRELASLLQLKTAMVQRESLAPMWSKLRIWDKRKPLYEAALSRLSLTQIEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LALSSTLELKLKQGGEEDWTLLTHLCLLFDASAHSALVDTRG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRVYPEQLGRHLTPLAQCYCLFGDDAWLIENARTQLLMAARQQGFEERISLEQDNGFNWN
CCCCHHHHCCCCCHHHHEEEEECCCEEEHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHH
DLQNEWQALSLFASRRIIELTLPQAKPGADGSAMLTSLMAQPNPDLLLILRGPKLAAEQT
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHC
NSKWFKALDNVGIYLPCNTPEGQQFFRWLDGRIAANRLMLDGDARQLLATLYEGNLLAAE
CHHHHHHHHCCCEEEECCCCCHHHHHHHHHCHHHHCEEEECCCHHHHHHHHHCCCCHHHH
QAVQLLALLAANRRISAEELSQYFDDQSRFNVFQLSDALLGNQQQRVAHILAQLKAEGTA
HHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
LPIVLWAVFRELASLLQLKTAMVQRESLAPMWSKLRIWDKRKPLYEAALSRLSLTQIEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LALSSTLELKLKQGGEEDWTLLTHLCLLFDASAHSALVDTRG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]