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The map label for this gene is prfA

Identifier: 119775698

GI number: 119775698

Start: 3071948

End: 3073039

Strand: Reverse

Name: prfA

Synonym: Sama_2566

Alternate gene names: 119775698

Gene position: 3073039-3071948 (Counterclockwise)

Preceding gene: 119775699

Following gene: 119775697

Centisome position: 71.36

GC content: 56.87

Gene sequence:

>1092_bases
ATGAAGGAATCCGTCATCCGCAAGCTGGAAGGCTTGCTCGAGCGCAACGAAGAAGTATTGGCGCTGCTCAGCGACGCCAG
CATCATTTCCGATCAGGACCGTTTTCGCGCCCTTTCCAAAGAATACGCCCAGCTTGAAGACGTGGTAAAAGGCTTCAAGG
CTTATCAGCAGGCGGTGGCCGACCTGGAAACCGCCAAAGAGATGCTCGAAGAGGACGATCCTGAGCTGAAAGAAATGGCC
CAGGAAGAAATCAAATCCGCCAAGGCGAGCCTCGAGCGGCTCGAGGCCGAGCTGCAAATTTTGCTGCTCCCTAAAGACCC
CAACGATGAAGCCAACGCGTTTGTGGAAATCCGTGCCGGAGCCGGTGGTGATGAAGCCGCCATTTTTGCCGGTGACCTGT
TCCGCATGTACAGCCGTTATGCCGAAACCCAGCGCTGGCAGGTGGAAATCATGAGCGCCAACGAAGGCGAACATGGTGGT
TATAAAGAAGTCATTGCACGTTTCAGCGGTGAGCGCGTTTACGGCAAACTCAAATTCGAGTCCGGTGGCCATAGAGTTCA
ACGGGTGCCTGAAACTGAATCCCAGGGTCGGGTGCACACCTCGGCCTGTACCGTAGCTGTGCTGCACGAAGTGCCCGAAG
CCGAAGCGGTGGAAATCAACCCATCTGAACTGCGCATCGACACTTTCCGCGCCTCCGGTGCCGGTGGGCAGCACGTTAAC
AAAACCGATTCGGCCATCCGTATTACCCACTTGCCCACAGGCACTGTGGTGGAGTGTCAGGATGAGCGCTCGCAGCACAA
AAACAAGGCTCGTGCCATGTCAGTGCTGGTGGCCCGTTTGCAGGCTGCCGAAGACGAGAAGCGCCGCAGCGCCGAAGCCA
CCACCCGACGGAATCTGGTAGGAAGCGGCGATCGTTCTGAACGTATCCGCACCTACAACTTCCCTCAGGGGCGGGTGAGC
GATCATCGCATCAACCTGACCCTGTATCGCCTCAATGAAGTGATGGAAGGCGATTTGAACGCCCTTATCGAACCCATAGT
ACAGGAACACCAGGCCGATTTGTTGGCGGCACTGGCAGACGAGCAGGGGTAA

Upstream 100 bases:

>100_bases
CAGGCCCTGACGACCGCCAGTCGTCAGGGGGACCTCAATACTCTGGGGCAACTTCGGACCGCGCTCGGCCTGGATAAACA
CTGAGACTTTTGAGAGTTAA

Downstream 100 bases:

>100_bases
AGCTTGGCAGCCCATTCCACTATAGGTGAAGCCTTGCAATGGGGCTTCAGCACCCTGGCCTCCAGCAGCACTTCTGCCAA
TCTGGATGCCGAGGTGCTGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESVIRKLEGLLERNEEVLALLSDASIISDQDRFRALSKEYAQLEDVVKGFKAYQQAVADLETAKEMLEEDDPELKEMA
QEEIKSAKASLERLEAELQILLLPKDPNDEANAFVEIRAGAGGDEAAIFAGDLFRMYSRYAETQRWQVEIMSANEGEHGG
YKEVIARFSGERVYGKLKFESGGHRVQRVPETESQGRVHTSACTVAVLHEVPEAEAVEINPSELRIDTFRASGAGGQHVN
KTDSAIRITHLPTGTVVECQDERSQHKNKARAMSVLVARLQAAEDEKRRSAEATTRRNLVGSGDRSERIRTYNFPQGRVS
DHRINLTLYRLNEVMEGDLNALIEPIVQEHQADLLAALADEQG

Sequences:

>Translated_363_residues
MKESVIRKLEGLLERNEEVLALLSDASIISDQDRFRALSKEYAQLEDVVKGFKAYQQAVADLETAKEMLEEDDPELKEMA
QEEIKSAKASLERLEAELQILLLPKDPNDEANAFVEIRAGAGGDEAAIFAGDLFRMYSRYAETQRWQVEIMSANEGEHGG
YKEVIARFSGERVYGKLKFESGGHRVQRVPETESQGRVHTSACTVAVLHEVPEAEAVEINPSELRIDTFRASGAGGQHVN
KTDSAIRITHLPTGTVVECQDERSQHKNKARAMSVLVARLQAAEDEKRRSAEATTRRNLVGSGDRSERIRTYNFPQGRVS
DHRINLTLYRLNEVMEGDLNALIEPIVQEHQADLLAALADEQG
>Mature_363_residues
MKESVIRKLEGLLERNEEVLALLSDASIISDQDRFRALSKEYAQLEDVVKGFKAYQQAVADLETAKEMLEEDDPELKEMA
QEEIKSAKASLERLEAELQILLLPKDPNDEANAFVEIRAGAGGDEAAIFAGDLFRMYSRYAETQRWQVEIMSANEGEHGG
YKEVIARFSGERVYGKLKFESGGHRVQRVPETESQGRVHTSACTVAVLHEVPEAEAVEINPSELRIDTFRASGAGGQHVN
KTDSAIRITHLPTGTVVECQDERSQHKNKARAMSVLVARLQAAEDEKRRSAEATTRRNLVGSGDRSERIRTYNFPQGRVS
DHRINLTLYRLNEVMEGDLNALIEPIVQEHQADLLAALADEQG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=47.5694444444444, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=41.839762611276, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.1139896373057, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=73.4806629834254, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=39.5209580838323, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=291, Percent_Identity=37.1134020618557, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=314, Percent_Identity=42.6751592356688, Blast_Score=245, Evalue=6e-66,
Organism=Drosophila melanogaster, GI19921226, Length=329, Percent_Identity=41.6413373860182, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SHEAM (A1S8R2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928438.1
- ProteinModelPortal:   A1S8R2
- SMR:   A1S8R2
- STRING:   A1S8R2
- GeneID:   4604813
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2566
- NMPDR:   fig|326297.7.peg.2406
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- PhylomeDB:   A1S8R2
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40543; Mature: 40543

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESVIRKLEGLLERNEEVLALLSDASIISDQDRFRALSKEYAQLEDVVKGFKAYQQAVA
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLETAKEMLEEDDPELKEMAQEEIKSAKASLERLEAELQILLLPKDPNDEANAFVEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAETQRWQVEIMSANEGEHGGYKEVIARFSGERVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVLHEVPEAEAVEINPSELRIDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHCCCCCEEEECHHHEEEEEEECCCCCCCCCC
KTDSAIRITHLPTGTVVECQDERSQHKNKARAMSVLVARLQAAEDEKRRSAEATTRRNLV
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSGDRSERIRTYNFPQGRVSDHRINLTLYRLNEVMEGDLNALIEPIVQEHQADLLAALAD
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EQG
CCC
>Mature Secondary Structure
MKESVIRKLEGLLERNEEVLALLSDASIISDQDRFRALSKEYAQLEDVVKGFKAYQQAVA
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLETAKEMLEEDDPELKEMAQEEIKSAKASLERLEAELQILLLPKDPNDEANAFVEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAETQRWQVEIMSANEGEHGGYKEVIARFSGERVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVLHEVPEAEAVEINPSELRIDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHCCCCCEEEECHHHEEEEEEECCCCCCCCCC
KTDSAIRITHLPTGTVVECQDERSQHKNKARAMSVLVARLQAAEDEKRRSAEATTRRNLV
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSGDRSERIRTYNFPQGRVSDHRINLTLYRLNEVMEGDLNALIEPIVQEHQADLLAALAD
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA