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The map label for this gene is hemK [H]

Identifier: 119775697

GI number: 119775697

Start: 3071087

End: 3071944

Strand: Reverse

Name: hemK [H]

Synonym: Sama_2565

Alternate gene names: 119775697

Gene position: 3071944-3071087 (Counterclockwise)

Preceding gene: 119775698

Following gene: 119775696

Centisome position: 71.34

GC content: 56.53

Gene sequence:

>858_bases
TTGGCAGCCCATTCCACTATAGGTGAAGCCTTGCAATGGGGCTTCAGCACCCTGGCCTCCAGCAGCACTTCTGCCAATCT
GGATGCCGAGGTGCTGCTGCAGCATTGCCTCGGTAAAAATCGCACTTTTCTTTACACCTGGCCTGAGCGACCACTGACCA
GCGAGCAGTGGAAACACTTCGAGCAGCTGATAAACCGCCGTGCCAAAGGTGTGCCGGTGGCGCATATTCTCGGTGAGCGT
GAGTTTTGGTCACTGAAGTTTTTGGTGAACGAAACCACCCTTATTCCACGGCCTGACACCGAGATGTTGGTTGAGACGGC
GCTTAATCTGCCGCTGCCCGACAACGCCAGAGTGCTGGACATGGGCACGGGAACCGGCGCCATTGCATTGGCGCTGGCCA
GCGAAAGGCCCAACTGGCGTATTACAGCACTGGATAAGGTGGATGATGCCGTCGCCCTGGCCAAGGCCAATCGTGAGCAA
CTGGGGTTGTCGCAGGTGGAAATCCTGCAAAGCGATTGGTTCAGTGCTGTACGGGATGCCGATTTCGACCTGATAGTCTC
CAATCCGCCTTATATCGATGAGCACGATGAGCATCTGGCGATGGGGGATGTGCGTTTTGAGCCTTTAAGTGCGCTCACTG
CCGCCGACGAAGGTTATGCGGACCTGAACCATATCGCCATGCACGCCCGTGAGCATCTCAAGGTGGGCGGGTATTTGCTG
CTCGAGCATGGTTTTGCCCAGGCGCTGAAACTCAGGGAAACTCTGGTATCCCTTGGGTACGACAAGGTGGCTACTGTGCG
GGATTTCGGTTCCAACGATCGCTGCACATTGGGGCTTTGGCATGGCGAGGATGCCTGA

Upstream 100 bases:

>100_bases
TGAAGTGATGGAAGGCGATTTGAACGCCCTTATCGAACCCATAGTACAGGAACACCAGGCCGATTTGTTGGCGGCACTGG
CAGACGAGCAGGGGTAAAGC

Downstream 100 bases:

>100_bases
GTGCGCTTGAGGCACACTTGGTCCGGGCAGCATTGGTAAGGGCTGAAGGATTCGGGTACACTAGCGCCTTTGTTTTTCGG
GCATTTAGTTTCAGGACTCT

Product: hemK family protein

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAAHSTIGEALQWGFSTLASSSTSANLDAEVLLQHCLGKNRTFLYTWPERPLTSEQWKHFEQLINRRAKGVPVAHILGER
EFWSLKFLVNETTLIPRPDTEMLVETALNLPLPDNARVLDMGTGTGAIALALASERPNWRITALDKVDDAVALAKANREQ
LGLSQVEILQSDWFSAVRDADFDLIVSNPPYIDEHDEHLAMGDVRFEPLSALTAADEGYADLNHIAMHAREHLKVGGYLL
LEHGFAQALKLRETLVSLGYDKVATVRDFGSNDRCTLGLWHGEDA

Sequences:

>Translated_285_residues
MAAHSTIGEALQWGFSTLASSSTSANLDAEVLLQHCLGKNRTFLYTWPERPLTSEQWKHFEQLINRRAKGVPVAHILGER
EFWSLKFLVNETTLIPRPDTEMLVETALNLPLPDNARVLDMGTGTGAIALALASERPNWRITALDKVDDAVALAKANREQ
LGLSQVEILQSDWFSAVRDADFDLIVSNPPYIDEHDEHLAMGDVRFEPLSALTAADEGYADLNHIAMHAREHLKVGGYLL
LEHGFAQALKLRETLVSLGYDKVATVRDFGSNDRCTLGLWHGEDA
>Mature_284_residues
AAHSTIGEALQWGFSTLASSSTSANLDAEVLLQHCLGKNRTFLYTWPERPLTSEQWKHFEQLINRRAKGVPVAHILGERE
FWSLKFLVNETTLIPRPDTEMLVETALNLPLPDNARVLDMGTGTGAIALALASERPNWRITALDKVDDAVALAKANREQL
GLSQVEILQSDWFSAVRDADFDLIVSNPPYIDEHDEHLAMGDVRFEPLSALTAADEGYADLNHIAMHAREHLKVGGYLLL
EHGFAQALKLRETLVSLGYDKVATVRDFGSNDRCTLGLWHGEDA

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=273, Percent_Identity=32.2344322344322, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787463, Length=262, Percent_Identity=53.4351145038168, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=33.502538071066, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=177, Percent_Identity=32.7683615819209, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582226, Length=275, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31652; Mature: 31521

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAHSTIGEALQWGFSTLASSSTSANLDAEVLLQHCLGKNRTFLYTWPERPLTSEQWKHF
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
EQLINRRAKGVPVAHILGEREFWSLKFLVNETTLIPRPDTEMLVETALNLPLPDNARVLD
HHHHHHHCCCCCHHHHHCCCCEEEEEEEEECEEECCCCCHHHHHHHHHCCCCCCCCEEEE
MGTGTGAIALALASERPNWRITALDKVDDAVALAKANREQLGLSQVEILQSDWFSAVRDA
ECCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHCC
DFDLIVSNPPYIDEHDEHLAMGDVRFEPLSALTAADEGYADLNHIAMHAREHLKVGGYLL
CCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEE
LEHGFAQALKLRETLVSLGYDKVATVRDFGSNDRCTLGLWHGEDA
EHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
AAHSTIGEALQWGFSTLASSSTSANLDAEVLLQHCLGKNRTFLYTWPERPLTSEQWKHF
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
EQLINRRAKGVPVAHILGEREFWSLKFLVNETTLIPRPDTEMLVETALNLPLPDNARVLD
HHHHHHHCCCCCHHHHHCCCCEEEEEEEEECEEECCCCCHHHHHHHHHCCCCCCCCEEEE
MGTGTGAIALALASERPNWRITALDKVDDAVALAKANREQLGLSQVEILQSDWFSAVRDA
ECCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHCC
DFDLIVSNPPYIDEHDEHLAMGDVRFEPLSALTAADEGYADLNHIAMHAREHLKVGGYLL
CCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEE
LEHGFAQALKLRETLVSLGYDKVATVRDFGSNDRCTLGLWHGEDA
EHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]