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The map label for this gene is ligA [H]

Identifier: 119775130

GI number: 119775130

Start: 2425326

End: 2426174

Strand: Reverse

Name: ligA [H]

Synonym: Sama_1995

Alternate gene names: 119775130

Gene position: 2426174-2425326 (Counterclockwise)

Preceding gene: 119775132

Following gene: 119775129

Centisome position: 56.34

GC content: 55.36

Gene sequence:

>849_bases
GTGCCACTTACCTCCAGTCTTGCGCCTTCGAATACGGCAACCGGCGCAAACCCGCCCAACAGCGCGGCTCCGCTTGCGGG
TTCACTGCAACTCGCATCCAGTAAGCCTTTACCCCCAGAGTCTGAGTTGCCTCGATATCTTGTAAGCGAAAAACTGGATG
GTGTCAGGGCCCGCTGGACAGGCCAAACGCTGGTCACCCGCTCTGGCAATCTAATCGCCCCGCCCGCCTGGTTTATTGCG
GCCTTGCCGGCAGATGTTCTCCTTGATGGCGAGTTATGGGCCGGCAGAGGCCGTTTTGAGTGGTTGAGTGGATTGGTACG
CTCACGCGGTCGTGATGATGATTGGTATCAGGTGCAATTAATGGTGTTTGACACACCCCAATCCGGGCTCGGATTTGCTG
AGCGACACAAGTGGTTGCAAGCTCGACTCTCCGACAAAACCGAGCCGCTCAAGCTGGTGGAACAAAGGCGCTTCCAGCAT
CTCTCTGAGCTCAATGACTATTATCAAGCCGTCATTCGAGGAGGGGGTGAAGGATTGATGCTGCATCGGGAAGATGCCCT
TTACATTGACGGTCGCAATCCACTGCTCATCAAGCTTAAGCCTCTGAATGATACCGAAGGGCAGGTGATTGGCTACCGTG
CGGGAAAAGGCCAATTTGAGGGCATGATGGGGGCGCTGCACCTGCGTCTTTTGGATGGCAGAGAGTTTTTTCTGGGCTCT
GGTTTCAGCTTTGATGAGCGCCGTCATCCTCCTGCCATTGGCGCTTGGGTCACTTTTCAATATCATGGGCTCACTGATAA
AGGGCTTCCCAAGGGCGCGCGATTTCTGCGTGTGCGTCCGATGGAATGA

Upstream 100 bases:

>100_bases
GCCCAGTATTGCCCTTAGGTTATTACAGGGTGTCAGCCGTGTTTGTTTCTTATTGTTCTGCCTGTGGCTGGCGCTGTTAC
CTTCGCATGTTCACTCGTCC

Downstream 100 bases:

>100_bases
ATTCCCGCAGATAAGAGACAAAGACACAAATGACCAGTACACCCGGTTCGATTCTATTTGTATGCATGGGCAATATCTGC
CGTTCTCCCAGCGCCGAAGC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPLTSSLAPSNTATGANPPNSAAPLAGSLQLASSKPLPPESELPRYLVSEKLDGVRARWTGQTLVTRSGNLIAPPAWFIA
ALPADVLLDGELWAGRGRFEWLSGLVRSRGRDDDWYQVQLMVFDTPQSGLGFAERHKWLQARLSDKTEPLKLVEQRRFQH
LSELNDYYQAVIRGGGEGLMLHREDALYIDGRNPLLIKLKPLNDTEGQVIGYRAGKGQFEGMMGALHLRLLDGREFFLGS
GFSFDERRHPPAIGAWVTFQYHGLTDKGLPKGARFLRVRPME

Sequences:

>Translated_282_residues
MPLTSSLAPSNTATGANPPNSAAPLAGSLQLASSKPLPPESELPRYLVSEKLDGVRARWTGQTLVTRSGNLIAPPAWFIA
ALPADVLLDGELWAGRGRFEWLSGLVRSRGRDDDWYQVQLMVFDTPQSGLGFAERHKWLQARLSDKTEPLKLVEQRRFQH
LSELNDYYQAVIRGGGEGLMLHREDALYIDGRNPLLIKLKPLNDTEGQVIGYRAGKGQFEGMMGALHLRLLDGREFFLGS
GFSFDERRHPPAIGAWVTFQYHGLTDKGLPKGARFLRVRPME
>Mature_281_residues
PLTSSLAPSNTATGANPPNSAAPLAGSLQLASSKPLPPESELPRYLVSEKLDGVRARWTGQTLVTRSGNLIAPPAWFIAA
LPADVLLDGELWAGRGRFEWLSGLVRSRGRDDDWYQVQLMVFDTPQSGLGFAERHKWLQARLSDKTEPLKLVEQRRFQHL
SELNDYYQAVIRGGGEGLMLHREDALYIDGRNPLLIKLKPLNDTEGQVIGYRAGKGQFEGMMGALHLRLLDGREFFLGSG
FSFDERRHPPAIGAWVTFQYHGLTDKGLPKGARFLRVRPME

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 31388; Mature: 31257

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTSSLAPSNTATGANPPNSAAPLAGSLQLASSKPLPPESELPRYLVSEKLDGVRARWT
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
GQTLVTRSGNLIAPPAWFIAALPADVLLDGELWAGRGRFEWLSGLVRSRGRDDDWYQVQL
CCEEEECCCCEECCHHHHHHHCCHHEEECCCEECCCCHHHHHHHHHHHCCCCCCEEEEEE
MVFDTPQSGLGFAERHKWLQARLSDKTEPLKLVEQRRFQHLSELNDYYQAVIRGGGEGLM
EEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHREDALYIDGRNPLLIKLKPLNDTEGQVIGYRAGKGQFEGMMGALHLRLLDGREFFLGS
EEECCEEEECCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHEEEEEEEECCCEEEEEC
GFSFDERRHPPAIGAWVTFQYHGLTDKGLPKGARFLRVRPME
CCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
PLTSSLAPSNTATGANPPNSAAPLAGSLQLASSKPLPPESELPRYLVSEKLDGVRARWT
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
GQTLVTRSGNLIAPPAWFIAALPADVLLDGELWAGRGRFEWLSGLVRSRGRDDDWYQVQL
CCEEEECCCCEECCHHHHHHHCCHHEEECCCEECCCCHHHHHHHHHHHCCCCCCEEEEEE
MVFDTPQSGLGFAERHKWLQARLSDKTEPLKLVEQRRFQHLSELNDYYQAVIRGGGEGLM
EEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHREDALYIDGRNPLLIKLKPLNDTEGQVIGYRAGKGQFEGMMGALHLRLLDGREFFLGS
EEECCEEEECCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHEEEEEEEECCCEEEEEC
GFSFDERRHPPAIGAWVTFQYHGLTDKGLPKGARFLRVRPME
CCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]