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The map label for this gene is pdhC [H]

Identifier: 119774847

GI number: 119774847

Start: 2090903

End: 2092486

Strand: Direct

Name: pdhC [H]

Synonym: Sama_1711

Alternate gene names: 119774847

Gene position: 2090903-2092486 (Clockwise)

Preceding gene: 119774846

Following gene: 119774848

Centisome position: 48.56

GC content: 56.76

Gene sequence:

>1584_bases
ATGATTAAAGATTTTATTTTGCCGGACATCGGTGAAGGTGTGGTCGAGTGCGAATTGGTCGAATGGCTGGTGAAAGAAGG
CGACCATATTGCTGAAGATCAGCCGATTTGTGACGTCATGACCGACAAAGCCCTGGTACAAATTCCGGCCCCGTTTGCCG
GTGTAGTCAGCAAGCTTTATTACGCCAAGGGTGAAATTGCCAAAGTGCATGCACCCTTGTATGCCGTTGAAATGGAAGGT
GAGGGCACCGATGCGTCAGCCGCTCCCGCCGAAGCCAATGCCCAAGCTGCGCACGATTCCGTACCCGTTGCCGCTCCTGT
GGCCGTTGCCGGTAAGCAGGTGGAAGATTTCTTGCTGCCCGATATTGGTGAAGGCATTGTGGAATGCGAACTGGTGGAGT
GGCTGGTAAACGAAGGCGATACGGTTGAAGAAGATCAGCCCATTGCCGACGTGATGACCGACAAGGCGTTGGTACAAATT
CCCGCCCTGAAAGCGGGCAAAATAGTCACTCTGCATTATCGCAAAGGACAACTCGCCAAGGTGCATGCGCCGCTGTATGC
CATCGAGGTGGACGCAGAGCATCCGGTAGTCCCTCCTGCCGCAGCGCCAGCAGCCGCAGCCAACCAGGCCGAGCGCGTTG
CTCCGTCTACCGCTGCTGTTAATGGAAATGGCAAGGCGCTGGCAAGCCCCGCGGTGCGCCGCATGGCCCGCAGTCTGGAT
GTGGATTTGTCGCTGGTACCCGGCAGCGGTAAGCATGGCCGTGTGTATAAAGAGGATATAGAGCAATACCTGAAGGGTGG
CGCTGCACCAGCCCCTGTTGCCCAGACTGCGGCTCCTCAAGCCGCAGTAACTCAATCTGCGCCTGTGCTGCCTGCTGCCG
ATGACAGGGTTGAGCCCATTCGCGGCGTGAAAGCGGCGATGGCGCGTCAGATGATGGACTCTGTGTCCAGCATCCCGCAC
TTTACCTACTGTGAAGAAATTGACCTGACTGAACTCGTTGCCCTGCGCGAGCGCATGAAGGCCAAGTACAGCAGTGATGA
TGTCAAGCTGACCATGATGCCGTTCTTCATGAAGTCGTTGTCGCTGGCTCTTACCGAGTTCCCCGTGGTGAATAGTCAGG
TGAATGCCGATTGCACTGAGCTGACCTACAAGGCCAGCCATAACATTGGTATGGCGGTGGACTCCAAGGTGGGTCTGCTG
GTACCTAACGTGAAGGATGTGCAGAGCAAGTCGATTCTGGATGTGGCCCGTGAAATCACCCGCCTTACCGACGCTGCCCG
CAGTGGTCGTGTCAGTCCGGCGGACCTTAAAGGCGGTACCATCTCCATCTCCAACATTGGCGCTCTGGGCGGCACTGTGG
CTACGCCTATTATCAACAAGCCTGAGGTGGCCATTGTGGCACTGGGTAAGCTGCAGACTCTGCCGCGCTTTGGCGCCGAT
GGCAGCGTTCAGGCCCGCAAGATAATGCAGGTAAGCTGGAGTGGCGATCACCGGGTGATTGATGGCGGCACCATTGCCCG
CTTCTGTAATCTGTGGAAGCAATATCTTGAGCAGCCAGAAGACATGCTGCTCGCCATGCGCTGA

Upstream 100 bases:

>100_bases
GGATACCCCGTATCCGCTTATCCACGAAAAAGAATACATGCCGGATGCGCTCAAGACATTTGAAGCCATCAAGGCCAGCG
TCAACTTCTAGGAGTCCGGT

Downstream 100 bases:

>100_bases
TAGCAAACAACTCTTCTTCAAAGGACGCCTGTGGCGTCCTTTGTTTTTTCGTATCGCTTGATTCGGCCGTACTCGACGCC
CCCGGGCGAAAACACCTTAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MIKDFILPDIGEGVVECELVEWLVKEGDHIAEDQPICDVMTDKALVQIPAPFAGVVSKLYYAKGEIAKVHAPLYAVEMEG
EGTDASAAPAEANAQAAHDSVPVAAPVAVAGKQVEDFLLPDIGEGIVECELVEWLVNEGDTVEEDQPIADVMTDKALVQI
PALKAGKIVTLHYRKGQLAKVHAPLYAIEVDAEHPVVPPAAAPAAAANQAERVAPSTAAVNGNGKALASPAVRRMARSLD
VDLSLVPGSGKHGRVYKEDIEQYLKGGAAPAPVAQTAAPQAAVTQSAPVLPAADDRVEPIRGVKAAMARQMMDSVSSIPH
FTYCEEIDLTELVALRERMKAKYSSDDVKLTMMPFFMKSLSLALTEFPVVNSQVNADCTELTYKASHNIGMAVDSKVGLL
VPNVKDVQSKSILDVAREITRLTDAARSGRVSPADLKGGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFGAD
GSVQARKIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPEDMLLAMR

Sequences:

>Translated_527_residues
MIKDFILPDIGEGVVECELVEWLVKEGDHIAEDQPICDVMTDKALVQIPAPFAGVVSKLYYAKGEIAKVHAPLYAVEMEG
EGTDASAAPAEANAQAAHDSVPVAAPVAVAGKQVEDFLLPDIGEGIVECELVEWLVNEGDTVEEDQPIADVMTDKALVQI
PALKAGKIVTLHYRKGQLAKVHAPLYAIEVDAEHPVVPPAAAPAAAANQAERVAPSTAAVNGNGKALASPAVRRMARSLD
VDLSLVPGSGKHGRVYKEDIEQYLKGGAAPAPVAQTAAPQAAVTQSAPVLPAADDRVEPIRGVKAAMARQMMDSVSSIPH
FTYCEEIDLTELVALRERMKAKYSSDDVKLTMMPFFMKSLSLALTEFPVVNSQVNADCTELTYKASHNIGMAVDSKVGLL
VPNVKDVQSKSILDVAREITRLTDAARSGRVSPADLKGGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFGAD
GSVQARKIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPEDMLLAMR
>Mature_527_residues
MIKDFILPDIGEGVVECELVEWLVKEGDHIAEDQPICDVMTDKALVQIPAPFAGVVSKLYYAKGEIAKVHAPLYAVEMEG
EGTDASAAPAEANAQAAHDSVPVAAPVAVAGKQVEDFLLPDIGEGIVECELVEWLVNEGDTVEEDQPIADVMTDKALVQI
PALKAGKIVTLHYRKGQLAKVHAPLYAIEVDAEHPVVPPAAAPAAAANQAERVAPSTAAVNGNGKALASPAVRRMARSLD
VDLSLVPGSGKHGRVYKEDIEQYLKGGAAPAPVAQTAAPQAAVTQSAPVLPAADDRVEPIRGVKAAMARQMMDSVSSIPH
FTYCEEIDLTELVALRERMKAKYSSDDVKLTMMPFFMKSLSLALTEFPVVNSQVNADCTELTYKASHNIGMAVDSKVGLL
VPNVKDVQSKSILDVAREITRLTDAARSGRVSPADLKGGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFGAD
GSVQARKIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPEDMLLAMR

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=45.5813953488372, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI31711992, Length=337, Percent_Identity=28.486646884273, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI203098816, Length=486, Percent_Identity=25.3086419753086, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI203098753, Length=449, Percent_Identity=26.0579064587973, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=31.7391304347826, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI260898739, Length=136, Percent_Identity=34.5588235294118, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786305, Length=537, Percent_Identity=33.147113594041, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=27.3170731707317, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=39.9532710280374, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=29.3269230769231, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=28.4116331096197, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=332, Percent_Identity=25.9036144578313, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=407, Percent_Identity=27.027027027027, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=23.1460674157303, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=40.8018867924528, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24582497, Length=307, Percent_Identity=26.7100977198697, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI20129315, Length=450, Percent_Identity=23.5555555555556, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645909, Length=248, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56082; Mature: 56082

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDHIAEDQPICDVMTDKALVQIPAPFAGVVSKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHH
YAKGEIAKVHAPLYAVEMEGEGTDASAAPAEANAQAAHDSVPVAAPVAVAGKQVEDFLLP
HCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHCCHHHHHHCC
DIGEGIVECELVEWLVNEGDTVEEDQPIADVMTDKALVQIPALKAGKIVTLHYRKGQLAK
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHEEECCCCCCCEEEEEECCCCEEE
VHAPLYAIEVDAEHPVVPPAAAPAAAANQAERVAPSTAAVNGNGKALASPAVRRMARSLD
EECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEECCHHHHHHHHHCC
VDLSLVPGSGKHGRVYKEDIEQYLKGGAAPAPVAQTAAPQAAVTQSAPVLPAADDRVEPI
CEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCHH
RGVKAAMARQMMDSVSSIPHFTYCEEIDLTELVALRERMKAKYSSDDVKLTMMPFFMKSL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
SLALTEFPVVNSQVNADCTELTYKASHNIGMAVDSKVGLLVPNVKDVQSKSILDVAREIT
HHHHHHCCCCCCCCCCCHHHEEEECCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHH
RLTDAARSGRVSPADLKGGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFGAD
HHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEEECHHHHCCCCCCC
GSVQARKIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPEDMLLAMR
CCCCEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCHHHEECC
>Mature Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDHIAEDQPICDVMTDKALVQIPAPFAGVVSKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHH
YAKGEIAKVHAPLYAVEMEGEGTDASAAPAEANAQAAHDSVPVAAPVAVAGKQVEDFLLP
HCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHCCHHHHHHCC
DIGEGIVECELVEWLVNEGDTVEEDQPIADVMTDKALVQIPALKAGKIVTLHYRKGQLAK
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHEEECCCCCCCEEEEEECCCCEEE
VHAPLYAIEVDAEHPVVPPAAAPAAAANQAERVAPSTAAVNGNGKALASPAVRRMARSLD
EECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEECCHHHHHHHHHCC
VDLSLVPGSGKHGRVYKEDIEQYLKGGAAPAPVAQTAAPQAAVTQSAPVLPAADDRVEPI
CEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCHH
RGVKAAMARQMMDSVSSIPHFTYCEEIDLTELVALRERMKAKYSSDDVKLTMMPFFMKSL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
SLALTEFPVVNSQVNADCTELTYKASHNIGMAVDSKVGLLVPNVKDVQSKSILDVAREIT
HHHHHHCCCCCCCCCCCHHHEEEECCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHH
RLTDAARSGRVSPADLKGGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFGAD
HHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEEECHHHHCCCCCCC
GSVQARKIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPEDMLLAMR
CCCCEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]